Integrated multi-trophic aquaculture (IMTA) has been proposed as a concept that combines the cultivation of fed aquaculture species (e.g., finfish/shrimp) with extractive aquaculture species (e.g., shellfish/seaweed)....Integrated multi-trophic aquaculture (IMTA) has been proposed as a concept that combines the cultivation of fed aquaculture species (e.g., finfish/shrimp) with extractive aquaculture species (e.g., shellfish/seaweed). In seaweed-based integrated aquaculture, seaweeds have the capacity to reduce the environmental impact of nitrogen-rich effluents on coastal ecosystems. Thus, selection of optimal species for such aquaculture is of great importance. The present study aimed to develop a seaweed species-selection index for selecting suitable species in seaweed-based integrated aquaculture system. The index was synthesized using available literature-based information, reference data, and physiological seaweed experiments to identify and prioritize the desired species. Undaria pinnatifida, Porphyra yezoensis and Ulva compressa scored the highest according to a seaweed-based integrated aquaculture suitability index (SASI). Seaweed species with the highest scores were adjudged to fit the integrated aquaculture systems. Despite the application of this model limited by local aquaculture environment, it is considered to be a useful tool for selecting seaweed species in IMTA.展开更多
Protoplast isolation was relevant for gene manipulation in U lva, and universal protocols have been proposed based on evaluation for various wildly collected species. However, only clonal laboratory cultures were prac...Protoplast isolation was relevant for gene manipulation in U lva, and universal protocols have been proposed based on evaluation for various wildly collected species. However, only clonal laboratory cultures were practical for genetic transformation, and whether applicability of such universal protocol existed for these artificial cultures has never been investigated. In this research, samples in different physiological states or developmental stages were tested in U. prolifera. The results proved that the protoplast yields were strongly dependent on the characteristics of samples. Neither F_v/F_m value nor chlorophyll content exhibited an ideal correlation with the protoplast yields. Alternatively, specific growth rate, coupled with developmental stage, could serve as an ef fective combined index to determine the right time for protoplast isolation. According to this instruction, here we reported the highest yields of protoplast((31.5±1.9)×10~6 cells/g f. wt.) in U. prolifera, following comparison between protocols, and further optimizations on enzyme content, incubation period, starting biomass and pretreatment. This specified protocol for artificially cultured clonal samples could meet the need for protoplast-mediated genetic transformation in U. prolifera.展开更多
Mesocosm hatcheries using semi-intensive culture methodologies are characterized by large tanks （40 m3） for fish larvae production at low densities （2-8 larvae/L）, with live prey production within the tank supplem...Mesocosm hatcheries using semi-intensive culture methodologies are characterized by large tanks （40 m3） for fish larvae production at low densities （2-8 larvae/L）, with live prey production within the tank supplemented by exogenous sources of feed. Due to the high biological quality of larvae produced and low level of technological input, these methodologies are regarded as an interesting option to meet the increasing fry demand from the aquaculture industry. This study aimed to describe the experience at Centro de Maricultura da Calheta, Madeira Island, Portugal, with the establishment of a mesocosm hatchery for the production of marine fish larvae. Production trials were initiated with gilthead seabream Sparus aurata. This larval species presented faster growth in total body length （TL） per day （TL = 3.7516e~ ~293day, R2 = 0.9404）, higher survival rate （SR = 31.9%） and comparable swimbladder inflation rates （SB = 90%） to those of larvae reared with intensive methodologies. High larval performance was obtained with two candidate species for aquaculture： the red porgy Pagruspagrus （TL = 3.212e~~39day, R2 = 0.995; SR = 15.3%; SB = 95.7%） and the white seabream Diplodus sargus （TL = 3.6355e~~413day, R2= 0.9824; SR = 25%; SB --- 100%）. Testing ofa 110 m3 mesocosm rearing tank with white seabream larvae revealed limitations associated to larger tanks namely, the amount and quality of live feed required daily. First trials with striped jack （Pseudocararoc dentex） larvae resulted in low larval survival （0.04%）, but the use of a white tank avoided culture management constraints related to positive phototropism. The present results suggest that a mesocosm using semi-intensive methodologies may contribute to species diversification and consequently, to the sustainable development of aquaculture.展开更多
The purpose of this paper falls within the theoretical framework of green marketing. It investigates the relationships that exist between the motives relating to aquacultural products marketed under the private label ...The purpose of this paper falls within the theoretical framework of green marketing. It investigates the relationships that exist between the motives relating to aquacultural products marketed under the private label of a traditional retailer （Coop）, the attitude towards their purchase, the perception of their quality, their reliability and the willingness to pay for them. Particular importance is given to the green motives, those that lead to a preference for a product made with specific renewable energies such as biogas. The structural equation models （SEM） technique is used to develop propositions representing a theoretical approach to the integration of green marketing, in the context of the process for purchasing Coop brand fish. With regard to distinguishing between the motives, we referred to the approach that sets out three different types of motive： clean, green and ethical. The resulting managerial implications make it possible to assess how much the different types of motive influence the choices of the consumers. Managers will benefit from the results of this paper by understanding better the benefits for consumers of aquacultural products from renewable energy such as biogas. The originality of the paper lies in its focusing on green purchase behaviour in a poorly investigated sector such as that of fish breeding. The paper contributes to an understanding of the factors that influence environmentally responsible purchase behaviour and their exact relationships, that are still unclear in literature. The fact that environmental issues and concerns are constantly changing implies that ongoing research into their influence on consumer behaviour is essential展开更多
The present study was designed to test the intra-specific crossing of two Egyptian populations from African catfish （Clarias gariepinus）, EI-Manzalah catfish （MCF） characterized by tolerates for poor water quality...The present study was designed to test the intra-specific crossing of two Egyptian populations from African catfish （Clarias gariepinus）, EI-Manzalah catfish （MCF） characterized by tolerates for poor water quality and easily farmed in captivity and EI-Qanater catfish （QCF） characterized by rapid growth and high quality flesh, for hybrid vigor, production and food processing to create new high quality products. Crosses of these populations were carried out in EI-Gamalia hatchery, EI-Manzalah, Egypt, during spring of 2008 between MCF~~ QCF~. African catfish hybrid, MCF and QCF fingerlings with a mean weight of 24.02 ＋ 1.38 g were stocked at a density of 50-fish per m3 in cages （112 m3 each） suspended in lake El-Manzalah. Fish were fed a formulated diet containing 31.20% crude protein and cultured for 214 days. The growth, survival and production data confirmed that catfish hybrid has been successful and showed a strong heterotic effect. Hybrid catfish had a significantly highest production, 47.5 kg per m3 and best feed conversion ratio （1.33） which demonstrates that cages are a viable alternative to ponds for the commercial production of African catfish and its hybrids. Recently, the African catfish market has required more processed products. The results indicated that： （1） there are slightly variations in African catfish population＇s products quality; and （2） the hot-smoked and fried-coated fish fillets products are more safe foods and acceptable to consumers due to their physic-chemical properties and microbial load acceptable results.展开更多
Marine invertebrates and fish are well known for their remarkable genetic diversity, which is commonly explained by large population size and the characteristic dispersive nature of their early, planktonic life histor...Marine invertebrates and fish are well known for their remarkable genetic diversity, which is commonly explained by large population size and the characteristic dispersive nature of their early, planktonic life history. Other potential sources of diversity in marine animals, such as a higher mutation rate, have been much less considered, though evidence for a high genetic load in marine bivalves has been accumulating for nearly half a century. In this review, I examine evidence for a higher genetic load in marine animals from studies of molecular marker segregation and linkage over the last 40 years, and survey recent work examining mutational load with molecular evolution approaches. Overall, marine animals appear to have higher genetic load than terrestrial animals （higher dn/ds ratios, inbreeding load, and segregation dis＇tortion）, though results are mixed for marine fish and data are lacking for many marine animal groups. Bivalves （oysters） have the highest loads observed among marine animals, comparable only to long-lived plants; however, more data is needed from other bivalves and more marine invertebrate taxa generally. For oysters, a higher load may be related to a chronically lower effective population size that, in concert with a higher mutational rate, elevate the number of deleterious mutations observed. I suggest that future studies use high-throughput sequencing approaches to examine （1） polymorphism in genomescale datasets across a wider range of marine animals at the population level and （2） intergenerational mutational changes between parents and offspring in crosses of aquaculture species to quantify mutation rates.展开更多
基金supported by the ‘Greenhouse Gas Emissions Reduction Using Seaweeds’ programthe Jeju Sea Grant College Program funded by the Korean Ministry of Land, Transport and Maritime Affairs, Republic of Korea
文摘Integrated multi-trophic aquaculture (IMTA) has been proposed as a concept that combines the cultivation of fed aquaculture species (e.g., finfish/shrimp) with extractive aquaculture species (e.g., shellfish/seaweed). In seaweed-based integrated aquaculture, seaweeds have the capacity to reduce the environmental impact of nitrogen-rich effluents on coastal ecosystems. Thus, selection of optimal species for such aquaculture is of great importance. The present study aimed to develop a seaweed species-selection index for selecting suitable species in seaweed-based integrated aquaculture system. The index was synthesized using available literature-based information, reference data, and physiological seaweed experiments to identify and prioritize the desired species. Undaria pinnatifida, Porphyra yezoensis and Ulva compressa scored the highest according to a seaweed-based integrated aquaculture suitability index (SASI). Seaweed species with the highest scores were adjudged to fit the integrated aquaculture systems. Despite the application of this model limited by local aquaculture environment, it is considered to be a useful tool for selecting seaweed species in IMTA.
基金Supported by the National Natural Science Foundation of China(No.41776153)the Scientific and Technological Innovation Project financially supported by Qingdao National Laboratory for Marine Science and Technology(No.2016ASKJ02-1)+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA11020304)the Postdoctoral Application Research Program funded by Qingdao(No.2016189)
文摘Protoplast isolation was relevant for gene manipulation in U lva, and universal protocols have been proposed based on evaluation for various wildly collected species. However, only clonal laboratory cultures were practical for genetic transformation, and whether applicability of such universal protocol existed for these artificial cultures has never been investigated. In this research, samples in different physiological states or developmental stages were tested in U. prolifera. The results proved that the protoplast yields were strongly dependent on the characteristics of samples. Neither F_v/F_m value nor chlorophyll content exhibited an ideal correlation with the protoplast yields. Alternatively, specific growth rate, coupled with developmental stage, could serve as an ef fective combined index to determine the right time for protoplast isolation. According to this instruction, here we reported the highest yields of protoplast((31.5±1.9)×10~6 cells/g f. wt.) in U. prolifera, following comparison between protocols, and further optimizations on enzyme content, incubation period, starting biomass and pretreatment. This specified protocol for artificially cultured clonal samples could meet the need for protoplast-mediated genetic transformation in U. prolifera.
文摘Mesocosm hatcheries using semi-intensive culture methodologies are characterized by large tanks （40 m3） for fish larvae production at low densities （2-8 larvae/L）, with live prey production within the tank supplemented by exogenous sources of feed. Due to the high biological quality of larvae produced and low level of technological input, these methodologies are regarded as an interesting option to meet the increasing fry demand from the aquaculture industry. This study aimed to describe the experience at Centro de Maricultura da Calheta, Madeira Island, Portugal, with the establishment of a mesocosm hatchery for the production of marine fish larvae. Production trials were initiated with gilthead seabream Sparus aurata. This larval species presented faster growth in total body length （TL） per day （TL = 3.7516e~ ~293day, R2 = 0.9404）, higher survival rate （SR = 31.9%） and comparable swimbladder inflation rates （SB = 90%） to those of larvae reared with intensive methodologies. High larval performance was obtained with two candidate species for aquaculture： the red porgy Pagruspagrus （TL = 3.212e~~39day, R2 = 0.995; SR = 15.3%; SB = 95.7%） and the white seabream Diplodus sargus （TL = 3.6355e~~413day, R2= 0.9824; SR = 25%; SB --- 100%）. Testing ofa 110 m3 mesocosm rearing tank with white seabream larvae revealed limitations associated to larger tanks namely, the amount and quality of live feed required daily. First trials with striped jack （Pseudocararoc dentex） larvae resulted in low larval survival （0.04%）, but the use of a white tank avoided culture management constraints related to positive phototropism. The present results suggest that a mesocosm using semi-intensive methodologies may contribute to species diversification and consequently, to the sustainable development of aquaculture.
文摘The purpose of this paper falls within the theoretical framework of green marketing. It investigates the relationships that exist between the motives relating to aquacultural products marketed under the private label of a traditional retailer （Coop）, the attitude towards their purchase, the perception of their quality, their reliability and the willingness to pay for them. Particular importance is given to the green motives, those that lead to a preference for a product made with specific renewable energies such as biogas. The structural equation models （SEM） technique is used to develop propositions representing a theoretical approach to the integration of green marketing, in the context of the process for purchasing Coop brand fish. With regard to distinguishing between the motives, we referred to the approach that sets out three different types of motive： clean, green and ethical. The resulting managerial implications make it possible to assess how much the different types of motive influence the choices of the consumers. Managers will benefit from the results of this paper by understanding better the benefits for consumers of aquacultural products from renewable energy such as biogas. The originality of the paper lies in its focusing on green purchase behaviour in a poorly investigated sector such as that of fish breeding. The paper contributes to an understanding of the factors that influence environmentally responsible purchase behaviour and their exact relationships, that are still unclear in literature. The fact that environmental issues and concerns are constantly changing implies that ongoing research into their influence on consumer behaviour is essential
文摘The present study was designed to test the intra-specific crossing of two Egyptian populations from African catfish （Clarias gariepinus）, EI-Manzalah catfish （MCF） characterized by tolerates for poor water quality and easily farmed in captivity and EI-Qanater catfish （QCF） characterized by rapid growth and high quality flesh, for hybrid vigor, production and food processing to create new high quality products. Crosses of these populations were carried out in EI-Gamalia hatchery, EI-Manzalah, Egypt, during spring of 2008 between MCF~~ QCF~. African catfish hybrid, MCF and QCF fingerlings with a mean weight of 24.02 ＋ 1.38 g were stocked at a density of 50-fish per m3 in cages （112 m3 each） suspended in lake El-Manzalah. Fish were fed a formulated diet containing 31.20% crude protein and cultured for 214 days. The growth, survival and production data confirmed that catfish hybrid has been successful and showed a strong heterotic effect. Hybrid catfish had a significantly highest production, 47.5 kg per m3 and best feed conversion ratio （1.33） which demonstrates that cages are a viable alternative to ponds for the commercial production of African catfish and its hybrids. Recently, the African catfish market has required more processed products. The results indicated that： （1） there are slightly variations in African catfish population＇s products quality; and （2） the hot-smoked and fried-coated fish fillets products are more safe foods and acceptable to consumers due to their physic-chemical properties and microbial load acceptable results.
文摘Marine invertebrates and fish are well known for their remarkable genetic diversity, which is commonly explained by large population size and the characteristic dispersive nature of their early, planktonic life history. Other potential sources of diversity in marine animals, such as a higher mutation rate, have been much less considered, though evidence for a high genetic load in marine bivalves has been accumulating for nearly half a century. In this review, I examine evidence for a higher genetic load in marine animals from studies of molecular marker segregation and linkage over the last 40 years, and survey recent work examining mutational load with molecular evolution approaches. Overall, marine animals appear to have higher genetic load than terrestrial animals （higher dn/ds ratios, inbreeding load, and segregation dis＇tortion）, though results are mixed for marine fish and data are lacking for many marine animal groups. Bivalves （oysters） have the highest loads observed among marine animals, comparable only to long-lived plants; however, more data is needed from other bivalves and more marine invertebrate taxa generally. For oysters, a higher load may be related to a chronically lower effective population size that, in concert with a higher mutational rate, elevate the number of deleterious mutations observed. I suggest that future studies use high-throughput sequencing approaches to examine （1） polymorphism in genomescale datasets across a wider range of marine animals at the population level and （2） intergenerational mutational changes between parents and offspring in crosses of aquaculture species to quantify mutation rates.
