Common aquaculture practices subject fish to a variety of acute and chronic stressors. Such stressors are inherent in aquaculture production but can adversely affect survival, growth, immune response, reproductive cap...Common aquaculture practices subject fish to a variety of acute and chronic stressors. Such stressors are inherent in aquaculture production but can adversely affect survival, growth, immune response, reproductive capacity, and behavior. Understanding the biological mechanisms underlying stress responses helps with methods to alleviate the negative effects through better aquaculture practices, resulting in improved animal welfare and production efficiency. In the present study, transcriptome sequencing of liver and kidney was performed in silver pomfret( Pampus argenteus) subjected to handling stress versus controls. A total of 162.19 million clean reads were assembled to 30 339 unigenes. The quality of the assembly was high, with an N50 length of 2 472 bases. For function classification and pathway assignment, the unigenes were categorized into three GO(gene ontology) categories, twenty-six clusters of egg NOG(evolutionary genealogy of genes: non-supervised orthologous groups) function categories, and thirty-eight KEGG(Kyoto Encyclopedia of Genes and Genomes) pathways. Stress affected different functional groups of genes in the tissues studied. Differentially expressed genes were mainly involved in metabolic pathways(carbohydrate metabolism, lipid metabolism, amino-acid metabolism, uptake of cofactors and vitamins, and biosynthesis of other secondary metabolites), environmental information processing(signaling molecules and their interactions), organismal systems(endocrine system, digestive system), and disease(immune, neurodegenerative, endocrine and metabolic diseases). This is the first reported analysis of genome-wide transcriptome in P. argenteus, and the findings expand our understanding of the silver pomfret genome and gene expression in association with stress. The results will be useful to future analyses of functional genes and studies of healthy artificial breeding in P. argenteus and other related fish species.展开更多
Wild and farmed fish generally dif fer in their nutritional composition. In this study, adult wild and farmed broadhead catfish( Clarias macrocephalus Günther, 1864) were collected and were assessed for various c...Wild and farmed fish generally dif fer in their nutritional composition. In this study, adult wild and farmed broadhead catfish( Clarias macrocephalus Günther, 1864) were collected and were assessed for various characteristics, namely morphometrics, digestive indexes, and flesh quality. The morphometrics(standard length, body depth, eye width, fin height and tentacle length) and the digestive indexes(intestosomatic index, digestosomatic index, perivisceral fat index and activities of pepsin and lipase) diff ered significantly between the groups(P <0.05) and can be used to distinguish wild fish from farmed fish. In terms of protein synthesis capacity and color, the flesh quality was similar between the groups. However, radical scavenging activities and reducing power were significantly higher in the wild fish than in the farmraised group. The thermal transition characteristics of sarcoplasmic proteins, as well as myosin denaturation enthalpy and fatty acid profiles(C18:2 n6, C20:0, C22:1 n9, C24:0, Σpolyunsaturated fatty acids, and Σn–6) also exhibited potential to enable calls about the fish origin. The proximate chemical composition of whole body did not diff er between the two fish populations. Our findings suggest bioindicators, in terms of morphometrics, digestive indexes and flesh quality, that can be used to identify the origin of fish for forensic purposes, of for conservation biology of this near threatened species. The new nutritional information may be of interest to marketing, consumers, and has a connection to nutritional eff ects on human health.展开更多
基金Supported by the Natural Science Foundation of Shanghai City(No.12ZR1455000)the Central Nonprofit Basic Scientific Research Project for the Scientific Research Institutes of China(No.East 2014Y01)
文摘Common aquaculture practices subject fish to a variety of acute and chronic stressors. Such stressors are inherent in aquaculture production but can adversely affect survival, growth, immune response, reproductive capacity, and behavior. Understanding the biological mechanisms underlying stress responses helps with methods to alleviate the negative effects through better aquaculture practices, resulting in improved animal welfare and production efficiency. In the present study, transcriptome sequencing of liver and kidney was performed in silver pomfret( Pampus argenteus) subjected to handling stress versus controls. A total of 162.19 million clean reads were assembled to 30 339 unigenes. The quality of the assembly was high, with an N50 length of 2 472 bases. For function classification and pathway assignment, the unigenes were categorized into three GO(gene ontology) categories, twenty-six clusters of egg NOG(evolutionary genealogy of genes: non-supervised orthologous groups) function categories, and thirty-eight KEGG(Kyoto Encyclopedia of Genes and Genomes) pathways. Stress affected different functional groups of genes in the tissues studied. Differentially expressed genes were mainly involved in metabolic pathways(carbohydrate metabolism, lipid metabolism, amino-acid metabolism, uptake of cofactors and vitamins, and biosynthesis of other secondary metabolites), environmental information processing(signaling molecules and their interactions), organismal systems(endocrine system, digestive system), and disease(immune, neurodegenerative, endocrine and metabolic diseases). This is the first reported analysis of genome-wide transcriptome in P. argenteus, and the findings expand our understanding of the silver pomfret genome and gene expression in association with stress. The results will be useful to future analyses of functional genes and studies of healthy artificial breeding in P. argenteus and other related fish species.
基金Supported by the Research Fund from the Faculty of Science(No.1-2557-02-005)the Graduate School Research Support Funding for Thesis of the Prince of Songkla University
文摘Wild and farmed fish generally dif fer in their nutritional composition. In this study, adult wild and farmed broadhead catfish( Clarias macrocephalus Günther, 1864) were collected and were assessed for various characteristics, namely morphometrics, digestive indexes, and flesh quality. The morphometrics(standard length, body depth, eye width, fin height and tentacle length) and the digestive indexes(intestosomatic index, digestosomatic index, perivisceral fat index and activities of pepsin and lipase) diff ered significantly between the groups(P <0.05) and can be used to distinguish wild fish from farmed fish. In terms of protein synthesis capacity and color, the flesh quality was similar between the groups. However, radical scavenging activities and reducing power were significantly higher in the wild fish than in the farmraised group. The thermal transition characteristics of sarcoplasmic proteins, as well as myosin denaturation enthalpy and fatty acid profiles(C18:2 n6, C20:0, C22:1 n9, C24:0, Σpolyunsaturated fatty acids, and Σn–6) also exhibited potential to enable calls about the fish origin. The proximate chemical composition of whole body did not diff er between the two fish populations. Our findings suggest bioindicators, in terms of morphometrics, digestive indexes and flesh quality, that can be used to identify the origin of fish for forensic purposes, of for conservation biology of this near threatened species. The new nutritional information may be of interest to marketing, consumers, and has a connection to nutritional eff ects on human health.
