Environmental endocrine disrupting chemicals （EDCs）, commonly found in the environment, come from industry and agriculture, including pesticides, fungicides, insecticides, herbicides, and other chemicals. Nowadays, ...Environmental endocrine disrupting chemicals （EDCs）, commonly found in the environment, come from industry and agriculture, including pesticides, fungicides, insecticides, herbicides, and other chemicals. Nowadays, more and more EDCs were released into the environment. EDCs go into water body via atmosphere sedi-mentation, surface runoff, soil eluviation, etc., so water body becomes the main place for existing. In order to attract scientific and public attention worldwide and to prevent EDCs pol ution, in this study we reviewed the classification of EDCs and their concentrations in natural water bodies, drinking water sources and water plants, and the reproductive toxicity of EDCs to fish were reviewed. EDCs could disturb the endocrine system and make reproductive organs and reproduction abnor-mal, resulting in fertility descending, reproduction function damage, community quan-tity decrease and even species extinction. In addition, EDCs could disrupt the homeostasis maintained by hormones, which would result in defects of neural de-velopment and abnormalities of the endocrine and reproductive systems. The exact molecular mechanisms have not been completely reported, but researches have suggested that multiple mechanisms were involved in the action of EDCs. Although there have been researches on the biohazard of EDCs, there stil exist problems of weakness in fundamental researches, difficulties in recognizing and identifying EDCs and high cost, which restraint the knowledge on them.展开更多
[Objective] The aim was to provide molecular basis for the identification of species in the moss family Bryaceae by the construction of inter-simple sequence repeats （ISSR） fingerprinting. [Method] In order to seek ...[Objective] The aim was to provide molecular basis for the identification of species in the moss family Bryaceae by the construction of inter-simple sequence repeats （ISSR） fingerprinting. [Method] In order to seek standardizing PCR reaction set-up, an orthogonal design was used to optimize ISSR-PCR amplification system of Bryaceae in five factors （Mg2＋, dNTPs, primer, DNA template, Taq DNA polymerase） at four levels respectively. [Result] A suitable ISSR reaction system was obtained, namely： 20 μl reaction system containing 5 ng of DNA template, 0.2 μmol/L primer, 2.25 mmol/L MgCl2, 0.6 U of Taq DNA polymerase, 0.4 mmol/L dNTPs. Proper annealing temperature was found at 48-50 ℃.The above system and six ISSR-PCR primers were used for the PCR amplification of 14 samples from Bryaceae and the related species in Mniaceae. A total of 86 bands were amplified, all showed polymorphism. NJ cluster analysis showed a star-shaped cladogram. [Conclusion] The results manifested that ISSR fingerprinting could provide the appropriate degree of polymorphism at low taxonomic level, so it would be a useful tool to provide additional evidence for resolving taxonomic relationships at the species level of Bryaceae.展开更多
Interconnected river system networks is a national water conservancy strategy in China and focus of research. Here we discuss the classification system, material and energy exchange between rivers and lakes, various d...Interconnected river system networks is a national water conservancy strategy in China and focus of research. Here we discuss the classification system, material and energy exchange between rivers and lakes, various dynamic flows and ecological functions of river-lake interconnected relationships. We then propose a novel method for the health assessment of river systems based on interconnected water system networks. In a healthy river system there is ＂material and energy exchange＂ and it is the first and foremost relationship of material and energy exchange between rivers and lakes. There are unobstructed various ＂flows＂ between rivers and lakes including material flows （water, dissolved substances, sediments, organisms and contaminants）, energy flows （water levels, flow and flow velocity）, information flows （information generated with water flows, organisms and human activities） and value flows （shipping, power generation, drinking and irrigation）. Under the influences of na- ture and human activity, various flows are connected by river-lake interconnection to carry material and energy exchange between rivers and lakes to achieve river-lake interactions. The material and energy exchange between rivers and lakes become one of the approaches and the direct driving forces of changes in river-lake interconnected relationships. The benignant changes in river-lake interconnected relationship tend to be in relatively steady state and in ideal dynamic balance.展开更多
Recently, environmental pressures along coasts have increased substantially. Classification of estuaries according to their sus- ceptibility to eutrophication nutrient load is a useful method to determine priority man...Recently, environmental pressures along coasts have increased substantially. Classification of estuaries according to their sus- ceptibility to eutrophication nutrient load is a useful method to determine priority management objects and to enforce control measures. Using historical monitoring data from 2007 to 2012, from 65 estuaries, including 101 estuarine monitoring sections and 260 coastal monitoring stations, a nutrient-driven phytoplankton dynamic model was developed based on the relationship among phytoplankton biomass, Total Nitrogen （TN） load and physical features of estuaries. The ecological filter effect of es- tuaries was quantified by introducing conversion efficiency parameter values into the model. Markov Chain Monte Carlo algo- rithm of Bayesian inference was then employed to estimate parameters in the mode/. The developed model fitted well to the observed chlorophyll, primary production, grazing, and sinking rates. The analysis suggests that an estuary with Q/V （the ratio of river flow to estuarine volume） greater than 2.0 per year and e （conversion efficiency ratio） less than 1.0 g C/g N can be classified as less susceptible to TN load, Q/V between 0.7 to 2.0 per year and e between 1.0 to 3.0 g C/g N as moderately sus- ceptible, and e greater than 3.0 g C/g N as very susceptible. The estuaries with Q/V less than 0.7 per year vary greatly in their susceptibility. The estuaries with high and moderate susceptibility accounted for 67% of all the analyzed estuaries. They have relatively high eutrophication risks and should be the focus of environmental supervision and pollution prevention.展开更多
基金Supported by the China Agriculture Research System(CARS49)~~
文摘Environmental endocrine disrupting chemicals （EDCs）, commonly found in the environment, come from industry and agriculture, including pesticides, fungicides, insecticides, herbicides, and other chemicals. Nowadays, more and more EDCs were released into the environment. EDCs go into water body via atmosphere sedi-mentation, surface runoff, soil eluviation, etc., so water body becomes the main place for existing. In order to attract scientific and public attention worldwide and to prevent EDCs pol ution, in this study we reviewed the classification of EDCs and their concentrations in natural water bodies, drinking water sources and water plants, and the reproductive toxicity of EDCs to fish were reviewed. EDCs could disturb the endocrine system and make reproductive organs and reproduction abnor-mal, resulting in fertility descending, reproduction function damage, community quan-tity decrease and even species extinction. In addition, EDCs could disrupt the homeostasis maintained by hormones, which would result in defects of neural de-velopment and abnormalities of the endocrine and reproductive systems. The exact molecular mechanisms have not been completely reported, but researches have suggested that multiple mechanisms were involved in the action of EDCs. Although there have been researches on the biohazard of EDCs, there stil exist problems of weakness in fundamental researches, difficulties in recognizing and identifying EDCs and high cost, which restraint the knowledge on them.
基金Supported by Natural Science Foundation of Hebei Province(C2006000147)Zhengzhou Science and Technology Program(10PTGN449-6)~~
文摘[Objective] The aim was to provide molecular basis for the identification of species in the moss family Bryaceae by the construction of inter-simple sequence repeats （ISSR） fingerprinting. [Method] In order to seek standardizing PCR reaction set-up, an orthogonal design was used to optimize ISSR-PCR amplification system of Bryaceae in five factors （Mg2＋, dNTPs, primer, DNA template, Taq DNA polymerase） at four levels respectively. [Result] A suitable ISSR reaction system was obtained, namely： 20 μl reaction system containing 5 ng of DNA template, 0.2 μmol/L primer, 2.25 mmol/L MgCl2, 0.6 U of Taq DNA polymerase, 0.4 mmol/L dNTPs. Proper annealing temperature was found at 48-50 ℃.The above system and six ISSR-PCR primers were used for the PCR amplification of 14 samples from Bryaceae and the related species in Mniaceae. A total of 86 bands were amplified, all showed polymorphism. NJ cluster analysis showed a star-shaped cladogram. [Conclusion] The results manifested that ISSR fingerprinting could provide the appropriate degree of polymorphism at low taxonomic level, so it would be a useful tool to provide additional evidence for resolving taxonomic relationships at the species level of Bryaceae.
基金National Natural Science Foundation of China(41361003)Science and Technology Project in Jiangxi Province Department of Education(GJJ14733)
文摘Interconnected river system networks is a national water conservancy strategy in China and focus of research. Here we discuss the classification system, material and energy exchange between rivers and lakes, various dynamic flows and ecological functions of river-lake interconnected relationships. We then propose a novel method for the health assessment of river systems based on interconnected water system networks. In a healthy river system there is ＂material and energy exchange＂ and it is the first and foremost relationship of material and energy exchange between rivers and lakes. There are unobstructed various ＂flows＂ between rivers and lakes including material flows （water, dissolved substances, sediments, organisms and contaminants）, energy flows （water levels, flow and flow velocity）, information flows （information generated with water flows, organisms and human activities） and value flows （shipping, power generation, drinking and irrigation）. Under the influences of na- ture and human activity, various flows are connected by river-lake interconnection to carry material and energy exchange between rivers and lakes to achieve river-lake interactions. The material and energy exchange between rivers and lakes become one of the approaches and the direct driving forces of changes in river-lake interconnected relationships. The benignant changes in river-lake interconnected relationship tend to be in relatively steady state and in ideal dynamic balance.
基金supported by Environmental Protection Public Welfare Project of China(Grant No.201309008)
文摘Recently, environmental pressures along coasts have increased substantially. Classification of estuaries according to their sus- ceptibility to eutrophication nutrient load is a useful method to determine priority management objects and to enforce control measures. Using historical monitoring data from 2007 to 2012, from 65 estuaries, including 101 estuarine monitoring sections and 260 coastal monitoring stations, a nutrient-driven phytoplankton dynamic model was developed based on the relationship among phytoplankton biomass, Total Nitrogen （TN） load and physical features of estuaries. The ecological filter effect of es- tuaries was quantified by introducing conversion efficiency parameter values into the model. Markov Chain Monte Carlo algo- rithm of Bayesian inference was then employed to estimate parameters in the mode/. The developed model fitted well to the observed chlorophyll, primary production, grazing, and sinking rates. The analysis suggests that an estuary with Q/V （the ratio of river flow to estuarine volume） greater than 2.0 per year and e （conversion efficiency ratio） less than 1.0 g C/g N can be classified as less susceptible to TN load, Q/V between 0.7 to 2.0 per year and e between 1.0 to 3.0 g C/g N as moderately sus- ceptible, and e greater than 3.0 g C/g N as very susceptible. The estuaries with Q/V less than 0.7 per year vary greatly in their susceptibility. The estuaries with high and moderate susceptibility accounted for 67% of all the analyzed estuaries. They have relatively high eutrophication risks and should be the focus of environmental supervision and pollution prevention.
