By investigating the shear effect on submerged cultivation of a traditional Chinese medicinal herb Ganoderma lucidum, a relatively high cell concentration of 13.8 g·L^-1 by dry mass was obtained in bioreactor at ...By investigating the shear effect on submerged cultivation of a traditional Chinese medicinal herb Ganoderma lucidum, a relatively high cell concentration of 13.8 g·L^-1 by dry mass was obtained in bioreactor at an impeller tip speed (ITS) of 0.51m·s^-1. At an ITS of 0.51,1.02 and 1.53m·s^-1, a maximal production titer of intracellular polysaccharide was 2.64, 2.20 and 2.28g·L^-1 and that of ganoderic acid was 306, 299 and 273g·L^-1, respectively. Under these ITSs, the maximal mean projected area of dispersed hypheue was 3.70, 2.54 and 2.13 × 10^4μm^2, and that of pellets was 0.91, 0.67 and 0.55 mm^2, respectively. The information obtained is useful for efficient submerged cultivation of mushrooms on a large scale.展开更多
基金Supported by the National Natural Science Foundation of China (No. 20076011, No. 20236040 and No. 20225619).
文摘By investigating the shear effect on submerged cultivation of a traditional Chinese medicinal herb Ganoderma lucidum, a relatively high cell concentration of 13.8 g·L^-1 by dry mass was obtained in bioreactor at an impeller tip speed (ITS) of 0.51m·s^-1. At an ITS of 0.51,1.02 and 1.53m·s^-1, a maximal production titer of intracellular polysaccharide was 2.64, 2.20 and 2.28g·L^-1 and that of ganoderic acid was 306, 299 and 273g·L^-1, respectively. Under these ITSs, the maximal mean projected area of dispersed hypheue was 3.70, 2.54 and 2.13 × 10^4μm^2, and that of pellets was 0.91, 0.67 and 0.55 mm^2, respectively. The information obtained is useful for efficient submerged cultivation of mushrooms on a large scale.
