活性维生素D3及锌、锰复合物改善水印蛋研究

本试验旨在研究在产蛋鸡日粮中添加活性维生素D3及锌、锰复合物对蛋壳质量的影响。试验材料含维生素D3复合物,可在肠道中经肠道消化酶水解获得活性维生素D3形式,直接驱动对钙、磷的吸收代谢及其他功能,而锌、锰微量元素具有改善蛋壳质量和缓减应激等作用。试验结果显示,对照组蛋壳厚度为0.317mm,试验组蛋壳厚度为0.348mm,显著高于对照组(P<0.05)。对照组蛋壳评分为1.82,试验组蛋壳评分为1.10,极显著降低(P<0.01)。此外,试验组裂纹蛋和水印蛋分别为3.3%和26.7%,与对照组相比均大幅降低(分别为23.3%和48.7%)。结果表明,蛋鸡产蛋料添加活性维生素D3及锌、锰复合物可显著提高蛋壳质量,改善水印蛋问题。

水印蛋评分体系的建立及螯合微量元素和抗氧化剂对水印蛋发生率的影响

本研究通过水印蛋和蛋壳透光性的相关性建立了评估水印蛋严重程度的评分体系,并结合该体系研究螯合微量元素和抗氧化剂对蛋鸡水印蛋发生率及生产性能的影响。试验选择有较高水印蛋发生率的1 600只34周龄蛋鸡,随机分为4组,分别为对照组、螯合微量元素组、螯合微量元素+抗氧化剂组和抗氧化剂组,在第37周统计采食量、产蛋率等生产性能指标,并在每个重复随机抽取15个鸡蛋测定蛋壳厚度和强度,从每个重复随机抽取30枚鸡蛋用水印蛋评分系统测定水印蛋的发生率及水印蛋评分。结果表明:螯合微量元素组与对照组相比极显著地降低了水印蛋的发生率和水印蛋评分(P<0.01),螯合微量元素+抗氧化剂组与对照组相比显著降低了水印蛋的发生率和水印蛋评分(P<0.05);抗氧化剂组与对照组相比水印蛋的发生率和水印蛋评分偏低(P>0.05);各组对蛋鸡产蛋率、采食量、料蛋比、蛋壳厚度和强度均无显著影响(P>0.05)。

Pin1 expression affects cell proliferation and apoptosis of SW620 cells in colorectal carcinoma

Objective:The aim of our study was to investigate the effects of Pin1 reduction on SW620 cell proliferation and apoptosis in human colorectal carcinoma.Methods:We constructed a plasmid of RNA interfering(shRNA) for Pin1 gene(pGenesl-1-Pin1),then the plasmid was transfected into colorectal carcinoma SW620 cells line by liposome mediation.The protein expression of Pin1 was tested by Western blotting.The proliferation rate was analyzed by MTT and the apoptotic rate of cells was tested by flow cytometry.In order to explain further the effect of Pin1 in SW620 cells,the protein level of Bcl-2 was analyzed by Western blotting.Results:pGenesil-1-Pin1 plasmid was successfully constructed and confirmed by sequencing.The protein relative levels of Pin1 were 0.06 ± 0.04 for the P-shRNA/SW620 cells,and 0.32 ± 0.09 for the P-Con/SW620 cells.The cell growth rate of SW620 cells was slower while the apoptotic rate was increased after transfection with pGenesil-Pin1 plasmid,and the apoptotic rate was 12.38% ± 1.55% for the P-shRNA/SW620 group.At the same time,we found that the protein expression of Bcl-2 was also reduced.The results were 0.13 ± 0.04 for the P-shRNA/SW620 cells,and 0.36 ± 0.08 for the P-Con/SW620 cells.Conclusion:Inhibited Pin1 expression may suppress the cell proliferation and promote apoptosis of colorectal carcinoma cells in vitro.