OBJECTIVE: In this study, we investigated the effects of ethanol extract of Hupiruisu Fang(HPRS), a formula of Traditional Chinese Medicine, on HCT116 cell line.METHODS: MKN45, A549, Hela and HCT116 cells were treated...OBJECTIVE: In this study, we investigated the effects of ethanol extract of Hupiruisu Fang(HPRS), a formula of Traditional Chinese Medicine, on HCT116 cell line.METHODS: MKN45, A549, Hela and HCT116 cells were treated with ethanol extract of HPRS alone or the extract plus 5-Fluorouracil(5-FU) for 48 h, and then the cell viabilities were measured using CCK-8Kit. The early apoptosis rate and total apoptosis rate in both HCT116 cells were evaluated by flow cytometry. The m RNA levels of apoptosis-related genes including caspase-3, caspase-8, Bcl2 and Baxwere detected by real-time quantitative polymerase chain reaction. Lastly, the protein activities and expressions of those apoptosis related genes were observed for further verifying the pro-apoptosis of the extract of HPRS.RESULTS: Ethanol extract of HPRS could significantly induce apoptosis in HCT116 cell line. Synergistic analysis revealed that the extract exhibited a significant effect upon 5-FU-associated cytotoxicity in the cell line.CONCLUSION: The ethanol extract of HPRS plus5-FU might have the potential to improve the treatment of colorectal cancer.展开更多
基金Supported by the Foundation of Baoshan Branch of Shuguang Hospital,Shanghai University of Traditional Chinese Medicine(Study on Moxibustion Treatment Effects of Serous G-CSF and Lymphocyte Upon Cyclophosphamide-induced Immunosuppression Rats,No.201411)the Shanghai Outstanding Young Physician Training Project(Free Research Project,No.2014)
文摘OBJECTIVE: In this study, we investigated the effects of ethanol extract of Hupiruisu Fang(HPRS), a formula of Traditional Chinese Medicine, on HCT116 cell line.METHODS: MKN45, A549, Hela and HCT116 cells were treated with ethanol extract of HPRS alone or the extract plus 5-Fluorouracil(5-FU) for 48 h, and then the cell viabilities were measured using CCK-8Kit. The early apoptosis rate and total apoptosis rate in both HCT116 cells were evaluated by flow cytometry. The m RNA levels of apoptosis-related genes including caspase-3, caspase-8, Bcl2 and Baxwere detected by real-time quantitative polymerase chain reaction. Lastly, the protein activities and expressions of those apoptosis related genes were observed for further verifying the pro-apoptosis of the extract of HPRS.RESULTS: Ethanol extract of HPRS could significantly induce apoptosis in HCT116 cell line. Synergistic analysis revealed that the extract exhibited a significant effect upon 5-FU-associated cytotoxicity in the cell line.CONCLUSION: The ethanol extract of HPRS plus5-FU might have the potential to improve the treatment of colorectal cancer.
