The fresh leaves of Combretum micranthum were harvested during the rainy season in Akwa Ibom State, washed and subjected to extraction by maceration in distilled water. Concentration was done with a rotary evaporator....The fresh leaves of Combretum micranthum were harvested during the rainy season in Akwa Ibom State, washed and subjected to extraction by maceration in distilled water. Concentration was done with a rotary evaporator. The extracts were tested for content of the following phytochemicals using standard methods: alkaloids, saponins, tannins, anthraquinones, cardiac glycosides, flavonoids and steroids. Antibacterial sensitivity test of the extract was carried out on S. aureus, B. subtilis and E. coli using agar well diffusion method and Ampiclox as control. The effect of storage time of the extract on its antibacterial activity was determined after the extract was kept at room temperature for 1 h, 24 h, 48 h and 72 h, respectively. Preparations of 200 mg/mL of the aqueous extract were made with additives as follows: (a) no additive; (b) methyl paraben (0.3%); (c) propyl paraben (0.3%); (d) sodium metabisulfite (0.01%); (e) A combination of methyl paraben (0.3%) and propyl paraben (0.3%); (f) A combination of methyl paraben (0.3%), propyl paraben (0.3%) and sodium metabisulfite (0.01%). A visual observation of the stabilized preparations was employed on daily basis for 30 days. Phytochemical analyses on the aqueous extract of the fresh leaves showed the absence of alkaloids, anthraquinones and flavonoids. Saponins, cardiac glycosides and steroids were present in moderate amounts and there was abundance of tannins. The extract had similar effect on both S. aureus and E. coli cultures but activity was very low with B. subtilis. When S. aureus activity increased with time of storage up to 48 hours, thereafter the activity started reducing. However, the activity of E. coli continued to increase with time (within the study period). Thus, it appears reasonable to store the extract for at least 48 hours before the use for enhanced activity.展开更多
This paper deals with the enantioseparation of phenylsuccinic acid(H2A)enantiomers by liquid-liquid reactive extraction usingβ-CD derivatives as aqueous selectors.Cyclodextrin and its derivatives can interact with gu...This paper deals with the enantioseparation of phenylsuccinic acid(H2A)enantiomers by liquid-liquid reactive extraction usingβ-CD derivatives as aqueous selectors.Cyclodextrin and its derivatives can interact with guest molecules selectively to form complexes with different stabilities.Cyclodextrin derivatives are not soluble in organic liquids,but highly soluble in water.In this work,hydroxypropyl-β-cyclodextrin(HP-β-CD),hydroxyethyl- β-cyclodextrin(HE-β-CD)and methyl-β-cyclodextrin(Me-β-CD)were selected as chiral selectors in aqueous phase for the reactive extraction of phenylsuccinic acid enantiomers from organic phase to aqueous phase.The results show that the efficiency of the extraction depends,often strongly,on a number of process variables,including the types of organic solvents andβ-CD derivatives,the concentrations of the extractants and H2A enantiomers,pH and temperature.HP-β-CD,HE-β-CD and Me-β-CD have stronger recognition abilities for R-phenylsuccinic acid than for S-phenylsuccinic acid.Among the three kinds ofβ-CD derivatives,HP-β-CD has the strongest separation ability. Excellent enantioseparation was achieved under the optimal conditions of pH of 2.5 and temperature of 5°C with a maximum enantioselectivity(a)of 2.38.Reactive extraction of enantiomers with hydrophilicβ-CD derivatives is of strong chiral separation ability and can be hopeful for separations of various enantiomers at a large-scale.展开更多
Wastewater containing high concentrations of phenol and sodium sulfate is generated in sebacic acid (SA) industry. Castor oil acid, a raw material for producing SA, can be used to extract phenol from wastewater in o...Wastewater containing high concentrations of phenol and sodium sulfate is generated in sebacic acid (SA) industry. Castor oil acid, a raw material for producing SA, can be used to extract phenol from wastewater in order to reduce the amount of phenol used in the process and discharge of phenol. The results show that the extrac- tion mechanism is that hydroxyl group of phenol is linked to carboxyl group of castor oil acid by hydrogen bond. The extraction process approaches equilibrium in 30 min. Extraction ratio increases with the increase of sodium sulfate and castor oil acid, and decreases as phenol increases. When the oil-water ratio is 1 : 3, the optimal distribu- tion coefficient of 40 is obtained. Phenol saturation concentration in castor oil acid is 1.03 mol.L-1 after extraction for 4 times. The equilibrium constant (Kex) at 25℃ is 8.41 and the endothermic enthalpy (AH) is 1.513 kJ.mo1-1. The Gibbs free energy (AG) is -5.277 kJ. tool-1 and the value of AS is calculated to be 22.3 J. mo1-1. K-1.展开更多
A pre-emulsified mixture of linseed and algae oils (15/10) and stabilized with 686 ppm of a lyophilized water extract of Melissa officinalis, was successfully applied in dry fermented sausages to increase the ω-3 P...A pre-emulsified mixture of linseed and algae oils (15/10) and stabilized with 686 ppm of a lyophilized water extract of Melissa officinalis, was successfully applied in dry fermented sausages to increase the ω-3 PUFA content. The objective of this work was to evaluate the stability of this modified formulation during the storage and to compare it to that of a traditional formulation. Traditional and modified products were stored during 90 days at 4 °C in aerobic conditions. Fatty acid profiles, TBARS and volatile compounds derived from oxidation were analyzed at 0, 30 and 90 days of storage. The fatty acid profiles did not significantly change along the storage period. The stabilizing effect of the natural antioxidants ofM. officinalis could contribute to detect no losses of to-3 PUFA in Modified (30 days: 2.13 g/100 g of product, 90 days: 2.33 g/100 g of product), whereas in Control products a slightly significant reduction was detected (30 days: 0.34 g/100 g of product, 90 days: 0.29 g/! 00 g of product). After 90 days, the increases of TBARS and hexanal content were much higher in Control than in Modified (Control: 1.41 mg MDA/kg & 17,915 ng dodecane/kg of dry matter; Modified: 0.48 mg MDA/kg & 2,496 ng dodecane/kg of dry matter). In conclusion, the lyophilized water extract of M. officinalis protected high ωo-3 PUFA of dry fermented sausages from oxidation along the storage time, guaranteeing the nutritional improvements achieved with the modified formulation.展开更多
Aqueous extracts from the native Chilean Quillaja saponaria tree are used orally and topically to treat lung inflammatory problems and toothache. This study aimed to corroborate their presumed anti-inflammatory activi...Aqueous extracts from the native Chilean Quillaja saponaria tree are used orally and topically to treat lung inflammatory problems and toothache. This study aimed to corroborate their presumed anti-inflammatory activity, identify their active ingredients and validate their use. The topical anti-inflammatory activity of a commercial partially purified Quillaja saponin aqueous extract (Ultra Dry 100Q, UD), the crude acid hydrolysate (H-100Q) of this material, its two most abundant sapogenins QA (quillaic acid) and PA (phytolaccagenic acid), and two formulations containing QA were subjected to topical assays for the inhibition ofmurine ear inflammation elicited by AA (arachidonic acid) or TPA (phorbol ester). The dose-dependent anti-inflammatory activity of QA was confirmed in both AA (maximal effect 92.1%) and TPA (maximal effect 62.2%) assays, and PA showed significant anti-inflammatory activity against AA (46.5%). Two dermo pharmaceutical formulations containing 8% w/v QA as the active ingredient--a cream and a gel--also exhibited significant anti-inflammatory effects in the TPA (50.8%) and AA (39.5%) assays.展开更多
Chenopodium album is an invasive weed in wheat fields in Egypt. The present study indicated the potentials for utilization of MILAE (Mangifera indica leaves aqueous extract) at different concentrations (5%, 10%, 15...Chenopodium album is an invasive weed in wheat fields in Egypt. The present study indicated the potentials for utilization of MILAE (Mangifera indica leaves aqueous extract) at different concentrations (5%, 10%, 15% and 25%) to suppress the germination and growth of C. album in Petri dish experiment Results indicated that predictable degrees of inhibition in C album germination and growth rate of seedlings were accompanied with the increase in the concentration of MILAE. PL (Plumule) and RL (radicle) lengths of C. album were negatively affected as the concentration of MILAE increased and the effect was prominent at 25% extract concentration. This study recommended the use of MILAE as strong phytotoxic potential allelochemicals. an alternative bioherbicides, since its leaves extract possess展开更多
Capparis spinosa L. is a traditionally used plant in Algerian folk medicine to treat asthma, rheumatism and other free radicals related disorders such inflammatory. The present study was designated to study the antiox...Capparis spinosa L. is a traditionally used plant in Algerian folk medicine to treat asthma, rheumatism and other free radicals related disorders such inflammatory. The present study was designated to study the antioxidant activity of aerial and root parts of Capparis spinosa L.. Total polyphenol content was determined, after extraction with solvents in five subfractions; methanol (CE), Hexan (HE) chloroform (CHE), ethyl acetate (EAE) and aqueous extracts (AE), employing the "Prussian blue assay". The results showed that EAE containing the highest concentration of phenolic compounds and Flavonoids followed by those of CHE of aerial and root parts, repectively. The antioxidant protective effects of extracts were determined by measuring the erythrocyte membrane resistance to free radical-induced. The results demonstrated that all the extracts exhibited a protective effect against t-BHP induced oxidative damages in human erythrocytes, the most potents wer CEs and CHEs with 86.55%, 85.81%, 74.59%, and 68.70%, for aerial and root parts, respectively, which were more effective than used standards: Quercetin (65.46%) and rutin (59.62%). In order to more clarify the previous antioxidants activity of the extracts through other mechanisms, the iron chelating ability assays were realized. The results showed that the iron chelating ability of Aerial and root extracts were very considerable; the CE showed an excellent chelating with ICs0 of 0.197 ± 0.031 μM/quercetin equivalent, higher than that used standard EDTA, with approximately 0.34 folds. These results show that Capparis spinosa L. extracts have strong anti-oxidant effects and may have some clinical benefits.展开更多
文摘The fresh leaves of Combretum micranthum were harvested during the rainy season in Akwa Ibom State, washed and subjected to extraction by maceration in distilled water. Concentration was done with a rotary evaporator. The extracts were tested for content of the following phytochemicals using standard methods: alkaloids, saponins, tannins, anthraquinones, cardiac glycosides, flavonoids and steroids. Antibacterial sensitivity test of the extract was carried out on S. aureus, B. subtilis and E. coli using agar well diffusion method and Ampiclox as control. The effect of storage time of the extract on its antibacterial activity was determined after the extract was kept at room temperature for 1 h, 24 h, 48 h and 72 h, respectively. Preparations of 200 mg/mL of the aqueous extract were made with additives as follows: (a) no additive; (b) methyl paraben (0.3%); (c) propyl paraben (0.3%); (d) sodium metabisulfite (0.01%); (e) A combination of methyl paraben (0.3%) and propyl paraben (0.3%); (f) A combination of methyl paraben (0.3%), propyl paraben (0.3%) and sodium metabisulfite (0.01%). A visual observation of the stabilized preparations was employed on daily basis for 30 days. Phytochemical analyses on the aqueous extract of the fresh leaves showed the absence of alkaloids, anthraquinones and flavonoids. Saponins, cardiac glycosides and steroids were present in moderate amounts and there was abundance of tannins. The extract had similar effect on both S. aureus and E. coli cultures but activity was very low with B. subtilis. When S. aureus activity increased with time of storage up to 48 hours, thereafter the activity started reducing. However, the activity of E. coli continued to increase with time (within the study period). Thus, it appears reasonable to store the extract for at least 48 hours before the use for enhanced activity.
基金Supported by the National Natural Science Foundation of China(20976041)the Program for New Century Excellent Talents in University,the Natural Science Foundation of Hunan Province(10JJ1004)the Open Fund Project of Key Laboratory in Hunan University(09K095)
文摘This paper deals with the enantioseparation of phenylsuccinic acid(H2A)enantiomers by liquid-liquid reactive extraction usingβ-CD derivatives as aqueous selectors.Cyclodextrin and its derivatives can interact with guest molecules selectively to form complexes with different stabilities.Cyclodextrin derivatives are not soluble in organic liquids,but highly soluble in water.In this work,hydroxypropyl-β-cyclodextrin(HP-β-CD),hydroxyethyl- β-cyclodextrin(HE-β-CD)and methyl-β-cyclodextrin(Me-β-CD)were selected as chiral selectors in aqueous phase for the reactive extraction of phenylsuccinic acid enantiomers from organic phase to aqueous phase.The results show that the efficiency of the extraction depends,often strongly,on a number of process variables,including the types of organic solvents andβ-CD derivatives,the concentrations of the extractants and H2A enantiomers,pH and temperature.HP-β-CD,HE-β-CD and Me-β-CD have stronger recognition abilities for R-phenylsuccinic acid than for S-phenylsuccinic acid.Among the three kinds ofβ-CD derivatives,HP-β-CD has the strongest separation ability. Excellent enantioseparation was achieved under the optimal conditions of pH of 2.5 and temperature of 5°C with a maximum enantioselectivity(a)of 2.38.Reactive extraction of enantiomers with hydrophilicβ-CD derivatives is of strong chiral separation ability and can be hopeful for separations of various enantiomers at a large-scale.
基金Supported by the National Natural Science Foundation of China (21006057) and the National Science Foundation for Post-doctoral Scientists of China (20100470351).
文摘Wastewater containing high concentrations of phenol and sodium sulfate is generated in sebacic acid (SA) industry. Castor oil acid, a raw material for producing SA, can be used to extract phenol from wastewater in order to reduce the amount of phenol used in the process and discharge of phenol. The results show that the extrac- tion mechanism is that hydroxyl group of phenol is linked to carboxyl group of castor oil acid by hydrogen bond. The extraction process approaches equilibrium in 30 min. Extraction ratio increases with the increase of sodium sulfate and castor oil acid, and decreases as phenol increases. When the oil-water ratio is 1 : 3, the optimal distribu- tion coefficient of 40 is obtained. Phenol saturation concentration in castor oil acid is 1.03 mol.L-1 after extraction for 4 times. The equilibrium constant (Kex) at 25℃ is 8.41 and the endothermic enthalpy (AH) is 1.513 kJ.mo1-1. The Gibbs free energy (AG) is -5.277 kJ. tool-1 and the value of AS is calculated to be 22.3 J. mo1-1. K-1.
文摘A pre-emulsified mixture of linseed and algae oils (15/10) and stabilized with 686 ppm of a lyophilized water extract of Melissa officinalis, was successfully applied in dry fermented sausages to increase the ω-3 PUFA content. The objective of this work was to evaluate the stability of this modified formulation during the storage and to compare it to that of a traditional formulation. Traditional and modified products were stored during 90 days at 4 °C in aerobic conditions. Fatty acid profiles, TBARS and volatile compounds derived from oxidation were analyzed at 0, 30 and 90 days of storage. The fatty acid profiles did not significantly change along the storage period. The stabilizing effect of the natural antioxidants ofM. officinalis could contribute to detect no losses of to-3 PUFA in Modified (30 days: 2.13 g/100 g of product, 90 days: 2.33 g/100 g of product), whereas in Control products a slightly significant reduction was detected (30 days: 0.34 g/100 g of product, 90 days: 0.29 g/! 00 g of product). After 90 days, the increases of TBARS and hexanal content were much higher in Control than in Modified (Control: 1.41 mg MDA/kg & 17,915 ng dodecane/kg of dry matter; Modified: 0.48 mg MDA/kg & 2,496 ng dodecane/kg of dry matter). In conclusion, the lyophilized water extract of M. officinalis protected high ωo-3 PUFA of dry fermented sausages from oxidation along the storage time, guaranteeing the nutritional improvements achieved with the modified formulation.
文摘Aqueous extracts from the native Chilean Quillaja saponaria tree are used orally and topically to treat lung inflammatory problems and toothache. This study aimed to corroborate their presumed anti-inflammatory activity, identify their active ingredients and validate their use. The topical anti-inflammatory activity of a commercial partially purified Quillaja saponin aqueous extract (Ultra Dry 100Q, UD), the crude acid hydrolysate (H-100Q) of this material, its two most abundant sapogenins QA (quillaic acid) and PA (phytolaccagenic acid), and two formulations containing QA were subjected to topical assays for the inhibition ofmurine ear inflammation elicited by AA (arachidonic acid) or TPA (phorbol ester). The dose-dependent anti-inflammatory activity of QA was confirmed in both AA (maximal effect 92.1%) and TPA (maximal effect 62.2%) assays, and PA showed significant anti-inflammatory activity against AA (46.5%). Two dermo pharmaceutical formulations containing 8% w/v QA as the active ingredient--a cream and a gel--also exhibited significant anti-inflammatory effects in the TPA (50.8%) and AA (39.5%) assays.
文摘Chenopodium album is an invasive weed in wheat fields in Egypt. The present study indicated the potentials for utilization of MILAE (Mangifera indica leaves aqueous extract) at different concentrations (5%, 10%, 15% and 25%) to suppress the germination and growth of C. album in Petri dish experiment Results indicated that predictable degrees of inhibition in C album germination and growth rate of seedlings were accompanied with the increase in the concentration of MILAE. PL (Plumule) and RL (radicle) lengths of C. album were negatively affected as the concentration of MILAE increased and the effect was prominent at 25% extract concentration. This study recommended the use of MILAE as strong phytotoxic potential allelochemicals. an alternative bioherbicides, since its leaves extract possess
文摘Capparis spinosa L. is a traditionally used plant in Algerian folk medicine to treat asthma, rheumatism and other free radicals related disorders such inflammatory. The present study was designated to study the antioxidant activity of aerial and root parts of Capparis spinosa L.. Total polyphenol content was determined, after extraction with solvents in five subfractions; methanol (CE), Hexan (HE) chloroform (CHE), ethyl acetate (EAE) and aqueous extracts (AE), employing the "Prussian blue assay". The results showed that EAE containing the highest concentration of phenolic compounds and Flavonoids followed by those of CHE of aerial and root parts, repectively. The antioxidant protective effects of extracts were determined by measuring the erythrocyte membrane resistance to free radical-induced. The results demonstrated that all the extracts exhibited a protective effect against t-BHP induced oxidative damages in human erythrocytes, the most potents wer CEs and CHEs with 86.55%, 85.81%, 74.59%, and 68.70%, for aerial and root parts, respectively, which were more effective than used standards: Quercetin (65.46%) and rutin (59.62%). In order to more clarify the previous antioxidants activity of the extracts through other mechanisms, the iron chelating ability assays were realized. The results showed that the iron chelating ability of Aerial and root extracts were very considerable; the CE showed an excellent chelating with ICs0 of 0.197 ± 0.031 μM/quercetin equivalent, higher than that used standard EDTA, with approximately 0.34 folds. These results show that Capparis spinosa L. extracts have strong anti-oxidant effects and may have some clinical benefits.