To construct the eukaryot ic expression system of E1 glycopr otein of rubella virus(RV)JR23 str ain and to study the biological an d immunological properties of the expressed product,the E1 gene was amplified by RT-PC...To construct the eukaryot ic expression system of E1 glycopr otein of rubella virus(RV)JR23 str ain and to study the biological an d immunological properties of the expressed product,the E1 gene was amplified by RT-PCR,sequenced and recombinated with the pBluescript ⅡSK+ vectorThe recombinated vecto r was co-transfected into BHK21 ce ll with vaccinia virus which carri es the gene of T7 RNA polymeraseT he expressed protein was characteri zed with hemadsorption,immunohisto chemistry and indirect immunofluor escence stainingThe results showe d that the recombinated vector,pBS K+-RV E1,was proved containing E1 gene by enzyme digestion and seque ncingThe expressed protein could be detected both in cytoplasm amd on membrane of the transfected cel ls by hemadsorption,immunohistoche mistry and indirect immunofluoresc enceThe E1 protein mainly focused in cytoplasm near the nucleusThes e data proved that the expression vector,pBSK+-RV E1,was successfull y constructed and the glycoprotein E1 of RV JR23 strain was effective ly expressed in BHK21 cell culture This study provides foundations f or studies on the relationships be tween structure and function of RV gene,between structure and functio n of RV proteins,and on the subuni t vaccine of rubella展开更多
动物水泡性口炎病毒(VSV)引起动物水泡性疾病。VSV有两个主要抗原群是核酸蛋白和G蛋白抗原。G蛋白因为能产生中和性抗体被认为是在发动抑制病毒感染方面起主要作用。已经证实VSV 的New Jersey 株中产生独立的中和性单克隆抗体的抗原决...动物水泡性口炎病毒(VSV)引起动物水泡性疾病。VSV有两个主要抗原群是核酸蛋白和G蛋白抗原。G蛋白因为能产生中和性抗体被认为是在发动抑制病毒感染方面起主要作用。已经证实VSV 的New Jersey 株中产生独立的中和性单克隆抗体的抗原决定蔟分布在G 蛋白的193～289氨基酸之间。展开更多
自1981年6月首次报告获得性免疫缺乏综合征(爱滋病 AIDS)以来,世界各国对本病的研究不断深入。现将近年来有关 AIDS 的病因学、发病原理、流行病学、诊断及其免疫预防与治疗,简要综述如下。一.病因学现在已知引起 AIDS 的病原体有两种,...自1981年6月首次报告获得性免疫缺乏综合征(爱滋病 AIDS)以来,世界各国对本病的研究不断深入。现将近年来有关 AIDS 的病因学、发病原理、流行病学、诊断及其免疫预防与治疗,简要综述如下。一.病因学现在已知引起 AIDS 的病原体有两种,即人免疫缺陷病毒(HIV)1型及2型,前者发现于1983年,1986年命名为 HIV—1,后者于1985年发现,命名为 HIV—2;二者又分别称为 HTLV—Ⅱ与HTLV—Ⅳ,皆属于逆转录病毒(retrovirus)科中的慢病毒(Lentivirus)亚科。展开更多
文摘To construct the eukaryot ic expression system of E1 glycopr otein of rubella virus(RV)JR23 str ain and to study the biological an d immunological properties of the expressed product,the E1 gene was amplified by RT-PCR,sequenced and recombinated with the pBluescript ⅡSK+ vectorThe recombinated vecto r was co-transfected into BHK21 ce ll with vaccinia virus which carri es the gene of T7 RNA polymeraseT he expressed protein was characteri zed with hemadsorption,immunohisto chemistry and indirect immunofluor escence stainingThe results showe d that the recombinated vector,pBS K+-RV E1,was proved containing E1 gene by enzyme digestion and seque ncingThe expressed protein could be detected both in cytoplasm amd on membrane of the transfected cel ls by hemadsorption,immunohistoche mistry and indirect immunofluoresc enceThe E1 protein mainly focused in cytoplasm near the nucleusThes e data proved that the expression vector,pBSK+-RV E1,was successfull y constructed and the glycoprotein E1 of RV JR23 strain was effective ly expressed in BHK21 cell culture This study provides foundations f or studies on the relationships be tween structure and function of RV gene,between structure and functio n of RV proteins,and on the subuni t vaccine of rubella
文摘自1981年6月首次报告获得性免疫缺乏综合征(爱滋病 AIDS)以来,世界各国对本病的研究不断深入。现将近年来有关 AIDS 的病因学、发病原理、流行病学、诊断及其免疫预防与治疗,简要综述如下。一.病因学现在已知引起 AIDS 的病原体有两种,即人免疫缺陷病毒(HIV)1型及2型,前者发现于1983年,1986年命名为 HIV—1,后者于1985年发现,命名为 HIV—2;二者又分别称为 HTLV—Ⅱ与HTLV—Ⅳ,皆属于逆转录病毒(retrovirus)科中的慢病毒(Lentivirus)亚科。
