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水流动力学注射PD-L1质粒在清髓预处理小鼠移植模型中的表达 被引量:1
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作者 李晓帆 李乃农 +1 位作者 杨凤娥 陈元仲 《中国实验血液学杂志》 CAS CSCD 北大核心 2015年第5期1432-1437,共6页
目的:建立水流动力学法表达PD-L1质粒小鼠模型并探讨清髓预处理对其表达的影响。方法:利用质粒扩增提取,应用尾静脉水流动力学注射法、门静脉胶原酶灌流分离肝细胞技术、RT-PCR、流式细胞术及ELISA法检测PD-L1的表达和功能;利用动物模... 目的:建立水流动力学法表达PD-L1质粒小鼠模型并探讨清髓预处理对其表达的影响。方法:利用质粒扩增提取,应用尾静脉水流动力学注射法、门静脉胶原酶灌流分离肝细胞技术、RT-PCR、流式细胞术及ELISA法检测PD-L1的表达和功能;利用动物模型了解化疗清髓或放射清髓对水流动力学注射PD-L1质粒表达的影响。结果:通过尾静脉大体积快速注射PD-L1质粒,在注射后8 h出现PD-L1 mRNA和蛋白的表达,24 h达峰值,7 d后回归基线;血清PD-L1在注射后24 h就达到了100μg/ml左右,7 d后达峰值,21 d内均保持高表达,1个月后回归基线;水流动力学注射所表达的PD-L1功能与文献报道一致;在各个时间点放/化疗清髓组与未预处理且转染的对照组相比PD-L1表达并无显著性差别;通过该方法注射表达PD-L1的小鼠移植后存活率高于对照组(P<0.05)。结论:小鼠清髓预处理对水流动力学注射PD-L1表达并无影响,注射PD-L1质粒可有效应用于小鼠造血干细胞移植模型中。 展开更多
关键词 造血干细胞移植 水流动力学法 PD-L1质粒 清髓预处理
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Accurate level set method for simulations of liquid atomization 被引量:4
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作者 邵长孝 罗坤 +2 位作者 杨建山 陈松 樊建人 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2015年第4期597-604,共8页
Computational fluid dynamics is an efficient numerical approach for spray atomization study, but it is challenging to accurately capture the gas-liquid interface. In this work, an accurate conservative level set metho... Computational fluid dynamics is an efficient numerical approach for spray atomization study, but it is challenging to accurately capture the gas-liquid interface. In this work, an accurate conservative level set method is intro- duced to accurately track the gas-liquid interfaces in liquid atomization. To validate the capability of this method, binary drop collision and drop impacting on liquid film are investigated. The results are in good agreement with experiment observations. In addition, primary atomization (swirling sheet atomization) is studied using this method. To the swirling sheet atomization, it is found that Rayleigh-Taylor instability in the azimuthal direction causes the primary breakup of liquid sheet and complex vortex structures are clustered around the rim of the liq- uid sheet. The effects of central gas velocity and liquid-gas density ratio on atomization are also investigated. This work lays a solid foundation for further studvin~ the mechanism of s^rav atomization. 展开更多
关键词 Computational fluid dynamicsLevel set methodSpray atomizationInterface captureBreakup
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Numerical Simulation of Countercurrent Flow in a PWR Hot Leg by Using Two-Fluid Model
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作者 Michio Murase Yoichi Utanohara +3 位作者 Chihiro Yanagi Takashi Takata Akira Yamaguchi Akio Tomiyama 《Journal of Energy and Power Engineering》 2013年第7期1215-1222,共8页
In order to evaluate CCFL (countercurrent flow limitation) characteristics in a PWR (pressurized water reactor) hot leg under reflux condensation, numerical simulations have been conducted using a 2F (two-fluid)... In order to evaluate CCFL (countercurrent flow limitation) characteristics in a PWR (pressurized water reactor) hot leg under reflux condensation, numerical simulations have been conducted using a 2F (two-fluid) model and a VOF (volume of fluid) method implemented in the CFD (computational fluid dynamics) software, FLUENT6.3.26. The 2F model gave good agreement with CCFL data in low pressure conditions but did not give good results for high pressure steam-water conditions. In the previous study, the computational grid and schemes were improved in the VOF method to improve calculations in circular tubes, and the calculated CCFL characteristics agreed well with the UPTF (Upper Plenum Test Facility) data at 1.5 MPa. In this study, therefore, using the 2F model and the computational grid previously improved for the VOF calculations, numerical simulations were conducted for steam-water flows at 1.5 MPa under PWR full-scale conditions. In the range of medium gas volumetric fluxes, the calculated CCFL characteristics agreed well with the values calculated by the VOF method and the UPTF data at 1.5 MPa. This indicated that the reference set of the interfacial drag correlations employed in this study could be applied not only to low pressures but also to high pressures. 展开更多
关键词 Reflux condensation PWR hot leg countercurrent flow numerical simulation two-fluid model.
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