Nitrification-denitrification losses of  ̄15N-labelled nitrate and ammonium applied to the rhizosphere andnonrhizosphere of flooded rice were evaluated in 2 greenhouse rhizobox experiments. The loss of added Nvia deni...Nitrification-denitrification losses of  ̄15N-labelled nitrate and ammonium applied to the rhizosphere andnonrhizosphere of flooded rice were evaluated in 2 greenhouse rhizobox experiments. The loss of added Nvia denitrification was estimated directly by measuring the total fluxes of (N_2O+N_2) ̄15N. It was found that 67% and51%-56% of  ̄15N-nitrate added to rice rhizosphere were lost as (N_2O+N_)- ̄15N in the 2 experiments, respectively,which were comparable to that added to nonrhizosphere soil (70%and47%, respectively), implying that tbedenitrifying activity in rice rhizosphere was as high as that in nonrhizosphere soil. However, only trace amounts(0-0.3% of added N) were recovered as (N_2O+N_2)- ̄15N when  ̄15N-ammonium was applied to either rhizosphere ornonrhizosphere, which seems to indicate that the nitrifying activity in the either rhizosphere or nonrhizosphere soilswas quite low. The apparent denitrification calculated from  ̄15N balance studies was 10%-47% higher than the totalflux of (N_2O+N_2)- ̄15N. Reasons for the large differences can not be explained satisfactorily. Though the denitrifyingactivity in rhizospbere was high and comparable to that in nonrhizosphere soil, presumably due to the low nitrifyingactivity and/ or the strong competition of N uptake against denitrification, the nitrification-denitrification takingplace in rhizosphere could not be an important mechanism of loss of ammonium N in flooded rice-soil system.展开更多
A pot experiment was conducted to investigate the effects of straw incorporation and soil pre-flooding on the fate of ( ̄(15)NH_4)_2SO_4-N and the growth of rice. Excessive application of rice straw when incorporated ...A pot experiment was conducted to investigate the effects of straw incorporation and soil pre-flooding on the fate of ( ̄(15)NH_4)_2SO_4-N and the growth of rice. Excessive application of rice straw when incorporated with ( ̄(15)NH_4)_2SO_4 at the C/N ratio of 40 reduced the loss of ( ̄(15)NH_4)_2SO_4-N and retarded the growth and development of rice significantly, while no adverse effects were observed on dry weight of panicle and the total recovery of ( ̄(15)NH_4)_2SO_4-N when rice straw was incorporated with ( ̄(15)NH_4)_2SO_4 at a C/N ratio less than 25. There were no significant effects of duration of soil pre-flooding within 6 weeks on ( ̄(15)NH_4)_2SO_4-Nuptake by rice and on rice growth, but, less loss of ( ̄(15)NH_4)_2SO_4-N was observed in the soil with a longer period of pre-flooding.展开更多
A pot experiment with exogenous cadmium was utilized to study the effects of waterlogging time on rice yield and Cd accumulationin different growth stages including top tillering stage and filling stage.The results sh...A pot experiment with exogenous cadmium was utilized to study the effects of waterlogging time on rice yield and Cd accumulationin different growth stages including top tillering stage and filling stage.The results showed that the rice yields of all flooding treatments were lower than the CK(CI).The WI,T1,T2,T3 and T4 decreased significantly by 23.7%,16.0%,15.5%,20.2%and 18.6%respectively.The Cd content of brown rice decreased with the extension of waterlogging time.And WF was the lowest,at only 3.4%of the wet irrigation of the whole growth period(WI).Under the same waterlogging condition,the Cd content in brown rice with 1 to 4 weeks of flooding treatment at the top tillering stage decreased by 27.1%(P﹤0.05),46.6%(P﹤0.05),56.0%(P﹤0.05)and 35.2%(P﹥0.05)respectively,compared with the treatment at the filling stage.And the average decrease was 41.2%.The variation tendency of Cd content in stems and leaves was similar to brown rice.The translocation efficiency of Cd from stems and leaves to rice seeds decreased with the extension of waterlogging time.The Cd enrichment factor of stems and leaves,as well as brown rice,varied greatly with different treatments.Specifically,the Cd enrichment factors in brown rice and in stems and leaves under WI were 28.0 and 17.8 times higher respectively than those under WF.The findings of this study demonstrated that flooding could inhibit the uptake and accumulation of Cd in rice,with significant positive correlation between them.The inhibition effect of flooding treatment on Cd accumulation in rice at the top tillering stage was superior to that at the filling stage.展开更多
Flooding/submergence of rice fields is a severe problem in South and South-East Asia, affecting more than 20 million hectares of rice every year. Submergence creates hypoxic or anoxic condition causing poor germinatio...Flooding/submergence of rice fields is a severe problem in South and South-East Asia, affecting more than 20 million hectares of rice every year. Submergence creates hypoxic or anoxic condition causing poor germination, seedling establishment,and enormous yield loss. Standing water in the field from weeks to months also leads to significant yield losses when large part of aerial tissues is under water. For flash flooding, a rice variety FR1A3 with tolerant gene(SUB1A) was identified. SNORKEL1 and SNORKEL2 have been identified for their ability to survive deep-water flooding by rapid elongation. Submergence stress has also been reported to adversely affect cell division and damage cellular and organelle membranes. Research on antioxidative enzymes response and genes that confer tolerance to prolonged flooding is in progress. Here we review the different anoxia responsive genes and the potential involvement of antioxidative enzymes, such as superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase, which occur in cells of rice plant exposed to submergence stress.展开更多
Vegetable production in South East Asia often is in rotation with flooded rice. The puddling of the soil with flooded rice production may result in unfavourable soil conditions for the subsequent production of dry lan...Vegetable production in South East Asia often is in rotation with flooded rice. The puddling of the soil with flooded rice production may result in unfavourable soil conditions for the subsequent production of dry land crops. To establish whether permanent vegetable production results in favourable soil conditions for vegetables, the effects of five different permanent vegetable production systems and a system of vegetable production in rotation with flooded rice on soil properties after flooded rice were studied in a 2-year field experiment. Bulk density at 0.05–0.10 m depth layer decreased with permanent vegetable production and vegetable production in rotation with flooded rice. The decrease in bulk density was influenced by the application of organic manure and rice husks, and especially by the number of crops cultivated, suggesting that frequency of soil tillage had a major effect on bulk density. Ploughing with buffalo traction after flooded rice, in combination with construction of raised beds, could reduce or totally eliminate negative effects of puddling on soil structure. Bulk density at 0.15–0.20 m soil depth was not influenced. Soil acidity decreased significantly in all systems. Soil organic carbon increased in all systems, but significant increase was only found in two permanent vegetable production systems. Available phosphorus(P) significantly increased in two permanent vegetable production systems, with a positively correlation to the amount of P applied. The significant decrease in bulk density and increase in p H(H2O), after only 2 years, showed that soil conditions after flooded rice could be improved in a short time under intensive vegetable production.展开更多
文摘Nitrification-denitrification losses of  ̄15N-labelled nitrate and ammonium applied to the rhizosphere andnonrhizosphere of flooded rice were evaluated in 2 greenhouse rhizobox experiments. The loss of added Nvia denitrification was estimated directly by measuring the total fluxes of (N_2O+N_2) ̄15N. It was found that 67% and51%-56% of  ̄15N-nitrate added to rice rhizosphere were lost as (N_2O+N_)- ̄15N in the 2 experiments, respectively,which were comparable to that added to nonrhizosphere soil (70%and47%, respectively), implying that tbedenitrifying activity in rice rhizosphere was as high as that in nonrhizosphere soil. However, only trace amounts(0-0.3% of added N) were recovered as (N_2O+N_2)- ̄15N when  ̄15N-ammonium was applied to either rhizosphere ornonrhizosphere, which seems to indicate that the nitrifying activity in the either rhizosphere or nonrhizosphere soilswas quite low. The apparent denitrification calculated from  ̄15N balance studies was 10%-47% higher than the totalflux of (N_2O+N_2)- ̄15N. Reasons for the large differences can not be explained satisfactorily. Though the denitrifyingactivity in rhizospbere was high and comparable to that in nonrhizosphere soil, presumably due to the low nitrifyingactivity and/ or the strong competition of N uptake against denitrification, the nitrification-denitrification takingplace in rhizosphere could not be an important mechanism of loss of ammonium N in flooded rice-soil system.
文摘A pot experiment was conducted to investigate the effects of straw incorporation and soil pre-flooding on the fate of ( ̄(15)NH_4)_2SO_4-N and the growth of rice. Excessive application of rice straw when incorporated with ( ̄(15)NH_4)_2SO_4 at the C/N ratio of 40 reduced the loss of ( ̄(15)NH_4)_2SO_4-N and retarded the growth and development of rice significantly, while no adverse effects were observed on dry weight of panicle and the total recovery of ( ̄(15)NH_4)_2SO_4-N when rice straw was incorporated with ( ̄(15)NH_4)_2SO_4 at a C/N ratio less than 25. There were no significant effects of duration of soil pre-flooding within 6 weeks on ( ̄(15)NH_4)_2SO_4-Nuptake by rice and on rice growth, but, less loss of ( ̄(15)NH_4)_2SO_4-N was observed in the soil with a longer period of pre-flooding.
基金Supported by Hunan Key Research and Development Program of Agricultural Technology Innovation(2016NK2190)National Key Research&Development Project(2016YFD0800705)Specialized Scientific Research in Public Welfare Sector Water Resources Ministry(201501019)~~
文摘A pot experiment with exogenous cadmium was utilized to study the effects of waterlogging time on rice yield and Cd accumulationin different growth stages including top tillering stage and filling stage.The results showed that the rice yields of all flooding treatments were lower than the CK(CI).The WI,T1,T2,T3 and T4 decreased significantly by 23.7%,16.0%,15.5%,20.2%and 18.6%respectively.The Cd content of brown rice decreased with the extension of waterlogging time.And WF was the lowest,at only 3.4%of the wet irrigation of the whole growth period(WI).Under the same waterlogging condition,the Cd content in brown rice with 1 to 4 weeks of flooding treatment at the top tillering stage decreased by 27.1%(P﹤0.05),46.6%(P﹤0.05),56.0%(P﹤0.05)and 35.2%(P﹥0.05)respectively,compared with the treatment at the filling stage.And the average decrease was 41.2%.The variation tendency of Cd content in stems and leaves was similar to brown rice.The translocation efficiency of Cd from stems and leaves to rice seeds decreased with the extension of waterlogging time.The Cd enrichment factor of stems and leaves,as well as brown rice,varied greatly with different treatments.Specifically,the Cd enrichment factors in brown rice and in stems and leaves under WI were 28.0 and 17.8 times higher respectively than those under WF.The findings of this study demonstrated that flooding could inhibit the uptake and accumulation of Cd in rice,with significant positive correlation between them.The inhibition effect of flooding treatment on Cd accumulation in rice at the top tillering stage was superior to that at the filling stage.
文摘Flooding/submergence of rice fields is a severe problem in South and South-East Asia, affecting more than 20 million hectares of rice every year. Submergence creates hypoxic or anoxic condition causing poor germination, seedling establishment,and enormous yield loss. Standing water in the field from weeks to months also leads to significant yield losses when large part of aerial tissues is under water. For flash flooding, a rice variety FR1A3 with tolerant gene(SUB1A) was identified. SNORKEL1 and SNORKEL2 have been identified for their ability to survive deep-water flooding by rapid elongation. Submergence stress has also been reported to adversely affect cell division and damage cellular and organelle membranes. Research on antioxidative enzymes response and genes that confer tolerance to prolonged flooding is in progress. Here we review the different anoxia responsive genes and the potential involvement of antioxidative enzymes, such as superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase, which occur in cells of rice plant exposed to submergence stress.
基金carried out in the framework of the‘Perm Veg’project(2007-2013)part of the strategic research program KB1"Global Food Security:Scarcity and Transition"which was funded by the Dutch Ministry of Economic Affairs,and carried out by Wageningen University and Research Centre
文摘Vegetable production in South East Asia often is in rotation with flooded rice. The puddling of the soil with flooded rice production may result in unfavourable soil conditions for the subsequent production of dry land crops. To establish whether permanent vegetable production results in favourable soil conditions for vegetables, the effects of five different permanent vegetable production systems and a system of vegetable production in rotation with flooded rice on soil properties after flooded rice were studied in a 2-year field experiment. Bulk density at 0.05–0.10 m depth layer decreased with permanent vegetable production and vegetable production in rotation with flooded rice. The decrease in bulk density was influenced by the application of organic manure and rice husks, and especially by the number of crops cultivated, suggesting that frequency of soil tillage had a major effect on bulk density. Ploughing with buffalo traction after flooded rice, in combination with construction of raised beds, could reduce or totally eliminate negative effects of puddling on soil structure. Bulk density at 0.15–0.20 m soil depth was not influenced. Soil acidity decreased significantly in all systems. Soil organic carbon increased in all systems, but significant increase was only found in two permanent vegetable production systems. Available phosphorus(P) significantly increased in two permanent vegetable production systems, with a positively correlation to the amount of P applied. The significant decrease in bulk density and increase in p H(H2O), after only 2 years, showed that soil conditions after flooded rice could be improved in a short time under intensive vegetable production.