为评价酶解与包合技术对破壁与未破壁蜂花粉水溶性蛋白质含量的影响,选用木瓜蛋白酶、菠萝蛋白酶、中性蛋白酶、柚苷酶、碱性蛋白酶、胃蛋白酶以及β-环糊精开展酶解与包合技术制备水溶性蜂花粉的比较试验。结果显示,采用β-环糊精处理...为评价酶解与包合技术对破壁与未破壁蜂花粉水溶性蛋白质含量的影响,选用木瓜蛋白酶、菠萝蛋白酶、中性蛋白酶、柚苷酶、碱性蛋白酶、胃蛋白酶以及β-环糊精开展酶解与包合技术制备水溶性蜂花粉的比较试验。结果显示,采用β-环糊精处理破壁蜂花粉,其产物的水溶性蛋白质含量最高(P<0.05),而碱性蛋白酶处理未破壁蜂花粉的效果显著高于其他处理(P<0.05);添加量、反应温度、时间及p H能够显著影响β-环糊精及碱性蛋白酶包合及酶解蜂花粉的效果,包合技术处理破壁蜂花粉的最佳工艺为环糊精添加量250.0 mg/g,反应温度37℃,反应时间5 h以及p H 8.7,碱性蛋白酶处理未破壁蜂花粉的最佳工艺为酶添加量1500 U/g,反应温度50℃,反应时间4 h以及p H 9.5。综上,该研究认为,β-环糊精及碱性蛋白酶处理能够显著提高蜂花粉的水溶性蛋白质含量,在实际生产中需根据蜂花粉不同的预处理情况选择合适的技术制备水溶性蜂花粉。展开更多
采用正交实验法首次研究高氨环境中马占相思栲胶的浅色化改性方法,探讨了氨水用量、过氧化氢用量、马占相思用量及反应温度等因素对改性马占相思溶液颜色的影响,同时考察了改性马占相思对天然胶乳蛋白质的固定作用.结果表明:改性处理后...采用正交实验法首次研究高氨环境中马占相思栲胶的浅色化改性方法,探讨了氨水用量、过氧化氢用量、马占相思用量及反应温度等因素对改性马占相思溶液颜色的影响,同时考察了改性马占相思对天然胶乳蛋白质的固定作用.结果表明:改性处理后,马占相思溶液颜色变浅,静置48 h颜色无明显改变,优选的改性条件为氨水量0.3份、过氧化氢1.0份、质量分数20%的马占相思栲胶溶液用量8份、保温反应温度80℃;改性马占相思具有固定胶乳蛋白质的作用,对于优化条件下制备的改性马占相思,当其用量为天然胶乳重量(干重)的1%时,可使天然橡胶膜中可提取水溶性蛋白质(EP)含量降至(45.58±6.27)μg/dm2,远小于ASTM D 3577-2009和GB 24788-2009标准对EP的限量值.展开更多
The effects of three nitrogen sources （ammonium, nitrate and ammonium＋nitrate） and three salt treatments （0, 100 and 200 mM NaCl） on nitrate reductase activity, proline, soluble protein and carbohydrate contents ...The effects of three nitrogen sources （ammonium, nitrate and ammonium＋nitrate） and three salt treatments （0, 100 and 200 mM NaCl） on nitrate reductase activity, proline, soluble protein and carbohydrate contents in psylliom （Plantago ovata） plants were Studied. The nitrate reductase activity tended to increase when NO3- was included in the root-zone solution, but decrease as salinity increased. All N sources stimulated plant growth and nutrient uptake. Shoot and root dry matter tended to decrease as salinity increased, but less so when both NH4＋ and NO3- were present. Shoot biomass accumulated to significantly greater quantities under the mixed-N treatments than when produced using either NH4＋ or NO3- N-form alone. Although not statistically significant, the root biomasses showed a similar tendency. Generally, leaf proline and soluble shoot carbohydrate contents increased with increasing salinity in contrast to soluble protein which decreased regardless of the N source. Under salinity conditions, the concentration of Na＋ in shoot and root tissues was highest in ammonium-N treatment, while that of K＋ was highest in the mixed-N treatment.展开更多
文摘为评价酶解与包合技术对破壁与未破壁蜂花粉水溶性蛋白质含量的影响,选用木瓜蛋白酶、菠萝蛋白酶、中性蛋白酶、柚苷酶、碱性蛋白酶、胃蛋白酶以及β-环糊精开展酶解与包合技术制备水溶性蜂花粉的比较试验。结果显示,采用β-环糊精处理破壁蜂花粉,其产物的水溶性蛋白质含量最高(P<0.05),而碱性蛋白酶处理未破壁蜂花粉的效果显著高于其他处理(P<0.05);添加量、反应温度、时间及p H能够显著影响β-环糊精及碱性蛋白酶包合及酶解蜂花粉的效果,包合技术处理破壁蜂花粉的最佳工艺为环糊精添加量250.0 mg/g,反应温度37℃,反应时间5 h以及p H 8.7,碱性蛋白酶处理未破壁蜂花粉的最佳工艺为酶添加量1500 U/g,反应温度50℃,反应时间4 h以及p H 9.5。综上,该研究认为,β-环糊精及碱性蛋白酶处理能够显著提高蜂花粉的水溶性蛋白质含量,在实际生产中需根据蜂花粉不同的预处理情况选择合适的技术制备水溶性蜂花粉。
文摘采用正交实验法首次研究高氨环境中马占相思栲胶的浅色化改性方法,探讨了氨水用量、过氧化氢用量、马占相思用量及反应温度等因素对改性马占相思溶液颜色的影响,同时考察了改性马占相思对天然胶乳蛋白质的固定作用.结果表明:改性处理后,马占相思溶液颜色变浅,静置48 h颜色无明显改变,优选的改性条件为氨水量0.3份、过氧化氢1.0份、质量分数20%的马占相思栲胶溶液用量8份、保温反应温度80℃;改性马占相思具有固定胶乳蛋白质的作用,对于优化条件下制备的改性马占相思,当其用量为天然胶乳重量(干重)的1%时,可使天然橡胶膜中可提取水溶性蛋白质(EP)含量降至(45.58±6.27)μg/dm2,远小于ASTM D 3577-2009和GB 24788-2009标准对EP的限量值.
文摘The effects of three nitrogen sources （ammonium, nitrate and ammonium＋nitrate） and three salt treatments （0, 100 and 200 mM NaCl） on nitrate reductase activity, proline, soluble protein and carbohydrate contents in psylliom （Plantago ovata） plants were Studied. The nitrate reductase activity tended to increase when NO3- was included in the root-zone solution, but decrease as salinity increased. All N sources stimulated plant growth and nutrient uptake. Shoot and root dry matter tended to decrease as salinity increased, but less so when both NH4＋ and NO3- were present. Shoot biomass accumulated to significantly greater quantities under the mixed-N treatments than when produced using either NH4＋ or NO3- N-form alone. Although not statistically significant, the root biomasses showed a similar tendency. Generally, leaf proline and soluble shoot carbohydrate contents increased with increasing salinity in contrast to soluble protein which decreased regardless of the N source. Under salinity conditions, the concentration of Na＋ in shoot and root tissues was highest in ammonium-N treatment, while that of K＋ was highest in the mixed-N treatment.
