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黄芩中3种黄酮水热法提取工艺的优化 被引量:14
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作者 李杰 尚孟文 +1 位作者 李婼楠 吴艳芳 《中成药》 CAS CSCD 北大核心 2020年第9期2427-2430,共4页
目的优化黄芩中黄芩苷、汉黄芩苷、黄芩素水热法提取工艺。方法在单因素试验基础上,以液料比、提取时间、提取温度为影响因素,3种黄酮总得率为评价指标,响应面法优化提取工艺。结果最佳条件为液料比40∶1,提取时间15 min,提取温度113℃,... 目的优化黄芩中黄芩苷、汉黄芩苷、黄芩素水热法提取工艺。方法在单因素试验基础上,以液料比、提取时间、提取温度为影响因素,3种黄酮总得率为评价指标,响应面法优化提取工艺。结果最佳条件为液料比40∶1,提取时间15 min,提取温度113℃,3种黄酮总得率为161 mg/g。结论水热法提取稳定可靠,可用于黄芩中黄芩苷、汉黄芩苷、黄芩素。 展开更多
关键词 黄芩 黄芩苷 汉黄芩苷 黄芩素 水热法提取 响应面法
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星点设计-响应面法优化枳实中黄酮类化合物的水热法提取工艺 被引量:9
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作者 李杰 李瑞芳 +3 位作者 张玉宾 王兵兵 冯标 闫德信 《药物分析杂志》 CAS CSCD 北大核心 2018年第8期1462-1469,共8页
目的:利用星点设计-响应面法优化枳实中黄酮类化合物的水热法提取工艺。方法:以黄酮类化合物(柚皮苷、橙皮苷和新橙皮苷)的得率为评价指标,通过单因素试验,研究提取温度、液料比及提取时间对枳实中黄酮类化合物得率的影响,采用响应面法... 目的:利用星点设计-响应面法优化枳实中黄酮类化合物的水热法提取工艺。方法:以黄酮类化合物(柚皮苷、橙皮苷和新橙皮苷)的得率为评价指标,通过单因素试验,研究提取温度、液料比及提取时间对枳实中黄酮类化合物得率的影响,采用响应面法优选水热法的最佳提取条件。结果:水热法提取黄酮类化合物的最佳条件:提取温度114℃,液料比66∶1,提取时间50 min;在此条件下黄酮类化合物的得率为19.82%,与预测值的偏差为0.56%。结论:采用星点设计-响应面法优选的水热法提取工艺,方法简便,预测性良好,为黄酮类及中药活性物质的提取提供了一条新途径。 展开更多
关键词 枳实 黄酮类化合物 水热法提取 星点设计-响应面法
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Different Carbon Fractions in Soils and Their Relationship with Trace Elements Content
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作者 Lubica Pospisilova Petr Skarpa Marie Konecna 《Journal of Life Sciences》 2011年第4期316-321,共6页
Luvisols, Stagnogleys and Cambisols, although less fertile, are used intensively for mixed farming, grazing and as forestland. Therefore we aimed our study at determination of total organic carbon (TOC) content, hum... Luvisols, Stagnogleys and Cambisols, although less fertile, are used intensively for mixed farming, grazing and as forestland. Therefore we aimed our study at determination of total organic carbon (TOC) content, humic substances (HS) content, humic acids (HA) content, fulvic acids (FA), hot water extractable carbon (Chw) content and content total and labile trace elements content. Humic substances quality was assessed by HA/FA ratio and by coloured indexes measured in ultraviolet and visible UV-VIS spectral range. The total and labile contents of Zn, Cd, Cu, Co, Pb, Mo and Se were determined by flame or electro-thermal atomic absorption spectrometry after extraction of the soil samples in the aqua regia (total content) and in the solution of 0.01 M CaCI2. Total and labile trace elements content was correlated with determined carbon fractions and soil reaction. Results showed that studied soils content low amount of TOC and had low quality of humic substances. HA/FA ratio was less than 1 and colour indexes were higher than 4. All determined carbon fractions correlated with labile form of Zn and Cd. Correlation between soil reaction and total zinc content was found. Significant effect of humic substances content on to water-soluble forms of heavy metals was detected. 展开更多
关键词 Humic acids fulvic acids hot water extractable carbon humus fractionation labile trace elements total trace elements.
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