Six ISSR primers are employed to display the polymorphism of different phases and sexes of red alga Gracilaria lemaneiformis, and two of them, P1 and P3, amplified distinct band patterns. The ISSR pattern amplified by...Six ISSR primers are employed to display the polymorphism of different phases and sexes of red alga Gracilaria lemaneiformis, and two of them, P1 and P3, amplified distinct band patterns. The ISSR pattern amplified by primer P1 of the female gametophyte is identical to that of tetrasporophyte, but distinct from that of male gametophyte. Of the bands produced by primer P3, one is specific to female gametophyte. Three morphologically similar fronds can be easily identified using ISSR technique. Two specific markers, SM1 andSF3, related to male gametophyte and female gametophyte, are cloned and sequenced. The homologous sequences of SM1 are found to encode a hypothetical protein. There is no homologous sequence of SF3 that can be found in GenBank.展开更多
Abstract Picoplankton distribution was investigated in different water masses of the East China Sea in November, 2006 and February, 2007. The autumn and winter cruises crossed three major water masses： the coastal wa...Abstract Picoplankton distribution was investigated in different water masses of the East China Sea in November, 2006 and February, 2007. The autumn and winter cruises crossed three major water masses： the coastal water mass （CWM）, the mixed water mass （MWM）, which forms on the continental shelf, and the Kuroshio water mass （KWM）. Picoplankton composition was resolved into four main groups by flow cytometry, namely Synechococcus, Prochlorococcus, picoeukaryotes, and heterotrophic bacteria. The average abundances of Synechococcus, picoeukaryotes, and heterotrophic bacteria were （0.63＋ 10.88）~ 103, （1.61＋1.16）x103, （3.39~1.27）x105 cells/mL in autumn and （6.45~8.60）x103, （3.23~2.63）x103, （3.76~1.37）x 105 cells/mL in winter, respectively. Prochlorococcus was not found in the CWM and seldom observed in surface samples in either season. However, Prochlorococcus was observed in the MWM and KWM （approximately 103 cells/mL） in both auttman and winter. Synechococcus distribution varied considerably among water masses, with the highest levels in KWM and lowest levels in CWM. The depth-averaged integrated abundance of Synechococcus was approximately 5-fold higher in KWM than in CWM, which may be due primarily to water temperature. In the MWM, Synechococcus was resolved as two subgroups; the presence of both subgroups was more common in autumn. Picoeukaryote abundance varied less among water masses than Synechococcus, and heterotrophic bacteria depth-averaged integrated abundance exhibited the smallest seasonal variations with respect to water mass. Correlation analysis showed that relationships between picoplankton abundances and environmental factors （temperature, nutrients, and chlorophyll a） differed among the three water masses, suggesting that the three water masses have different effects on picoplankton distribution （particularly Synechococcus）.展开更多
基金supported by the National Natural Science Foundation of China(No.30170736)the Key Experimental Marine Biology Laboratory of the Institute of 0ceanology,the Chinese Academy of Sciences.
文摘Six ISSR primers are employed to display the polymorphism of different phases and sexes of red alga Gracilaria lemaneiformis, and two of them, P1 and P3, amplified distinct band patterns. The ISSR pattern amplified by primer P1 of the female gametophyte is identical to that of tetrasporophyte, but distinct from that of male gametophyte. Of the bands produced by primer P3, one is specific to female gametophyte. Three morphologically similar fronds can be easily identified using ISSR technique. Two specific markers, SM1 andSF3, related to male gametophyte and female gametophyte, are cloned and sequenced. The homologous sequences of SM1 are found to encode a hypothetical protein. There is no homologous sequence of SF3 that can be found in GenBank.
基金Supported by the National Basic Research Program of China(973 Program)(No.2011CB409804)the National Natural Science Foundation of China for Creative Research Groups(No.41121064)the Knowledge Innovation Program of China(No.KZCX2-YW-Q07-02)
文摘Abstract Picoplankton distribution was investigated in different water masses of the East China Sea in November, 2006 and February, 2007. The autumn and winter cruises crossed three major water masses： the coastal water mass （CWM）, the mixed water mass （MWM）, which forms on the continental shelf, and the Kuroshio water mass （KWM）. Picoplankton composition was resolved into four main groups by flow cytometry, namely Synechococcus, Prochlorococcus, picoeukaryotes, and heterotrophic bacteria. The average abundances of Synechococcus, picoeukaryotes, and heterotrophic bacteria were （0.63＋ 10.88）~ 103, （1.61＋1.16）x103, （3.39~1.27）x105 cells/mL in autumn and （6.45~8.60）x103, （3.23~2.63）x103, （3.76~1.37）x 105 cells/mL in winter, respectively. Prochlorococcus was not found in the CWM and seldom observed in surface samples in either season. However, Prochlorococcus was observed in the MWM and KWM （approximately 103 cells/mL） in both auttman and winter. Synechococcus distribution varied considerably among water masses, with the highest levels in KWM and lowest levels in CWM. The depth-averaged integrated abundance of Synechococcus was approximately 5-fold higher in KWM than in CWM, which may be due primarily to water temperature. In the MWM, Synechococcus was resolved as two subgroups; the presence of both subgroups was more common in autumn. Picoeukaryote abundance varied less among water masses than Synechococcus, and heterotrophic bacteria depth-averaged integrated abundance exhibited the smallest seasonal variations with respect to water mass. Correlation analysis showed that relationships between picoplankton abundances and environmental factors （temperature, nutrients, and chlorophyll a） differed among the three water masses, suggesting that the three water masses have different effects on picoplankton distribution （particularly Synechococcus）.
