“水盆子”及水肥一体化在高标准农田建设中的应用

为进一步践行金塔县农业节水措施,金塔县2023年高标准农田项目通过建设“水盆子”工程进行调蓄,联合鸳鸯池水库及大墩门水闸枢纽增加地表水调节能力,提高指标内地表水利用率,同时实现与现状及后期规划高标准农田田间工程连接,通过管网向2.9×10^(4)亩水肥一体化高标准农田提供灌溉用水,这一工程措施对金塔县农业节水以及用水结构优化等方面具有重要的意义。

覆盆子水煎剂对H_2O_2诱导的bEnd.3细胞氧化应激损伤的影响

目的:探讨覆盆子水煎剂对H_2O_2诱导的小鼠脑微血管内皮细胞(b End.3细胞)氧化应激损伤的影响。方法:用不同浓度的H_2O_2处理b End.3细胞6 h,构建氧化应激模型;MTT法检测不同浓度的覆盆子水煎剂对b End.3细胞活性的影响;采用Western blot法检测相关凋亡蛋白Bax、Bcl-2、Cytochrome(Cyt-c)、Caspase 3、Caspase 8、Caspase 12蛋白的表达情况。结果:MTT检测发现覆盆子水煎剂可提高H_2O_2干预下的b End.3细胞活性,并且该保护作用呈现浓度依赖性(P<0.05)。Western blot检测结果显示覆盆子水煎剂可抑制Cyt-c高表达,上调Bcl-2/Bax比值,同时下调Cleaved-caspase 3/pro-caspase 3、Cleaved-caspase 8/pro-caspase 8、Cleaved-caspase 12/pro-caspase 12的比值(P<0.05)。结论:实验剂量的覆盆子水煎剂对H_2O_2介导的b End.3细胞损伤具有保护作用,主要与调节相关凋亡蛋白Bcl-2、Bax、Cyt-c的表达及Caspase 3、8、12活化有关。

覆盆子水提取液对鲜切苹果褐变的抑制作用

鲜切苹果在削皮、切分、保鲜等过程中会产生一定的损伤,导致褐变,降低感官品质和市场价值。采用覆盆子水提取液处理鲜切苹果,研究适宜的处理技术及褐变相关联的生理指标变化。结果表明:覆盆子水提取液的最佳浸提时间为2 h,最佳处理浓度为稀释5倍,最佳处理时间为10 min。覆盆子水提取液可抑制鲜切苹果多酚氧化酶、过氧化物酶和苯丙氨酸解氨酶的活性,经覆盆子水提取液处理后,鲜切苹果总酚含量降低,DPPH自由基清除活性增加。通过高效液相色谱分析发现覆盆子水提取液中含有阿魏酸、儿茶素、绿原酸、芦丁、p-香豆酸等活性成分,与抑制褐变密切相关。

覆盆子水煎液对维甲酸致小鼠骨质疏松模型影响

目的:通过维甲酸法建立小鼠骨质疏松动物模型,探究覆盆子水煎液对骨质疏松小鼠血清及胫骨力学强度等各项指标的影响。方法:将小鼠分别设置为空白组、模型组、阳性药仙灵骨葆胶囊7.5g/kg组、覆盆子水煎液6g/kg、3g/kg、1.5g/kg组。除空白组外,各给药组小鼠灌胃维甲酸CMC悬液,连续给药14天建立骨质疏松模型。造模成功后各组给予相应药物14天,末次药后,所有动物眶静脉取血,离心分离血清,观察血清中血清钙(Ca)、磷(P)、血清骨钙素(BGP)水平;检测小鼠体重、活动情况;取小鼠右侧股骨及胫骨进行病理学检查、骨密度测定及骨生物力学测定。结果:与模型组比,覆盆子水煎液各组以及仙灵骨葆组均可显著升高血Ca、骨性强度、骨密度,降低血P水平;覆盆子水煎液6g/kg、3g/kg组以及仙灵骨葆组均可显著降低血清中骨钙素水平,覆盆子水煎液各组明显改善股骨病理形态。结论:覆盆子水煎液对维甲酸致小鼠骨质疏松模型有较好的干预作用。

Fertility preservation after surgical transposition of the ovaries for pelvic tumors

Objective: The aim of the study was to assess the hormonal profile level in females with pelvic tumors after surgical transposition of the ovaries during surgery followed by postoperative radiotherapy to the pelvis. Methods: Fifteen patients with rectal, bladder and cervical cancer were included in the study with their age ranged between 25 and 40 years old, 7 patients with cancer rectum, 3 patients with bladder cancer and 5 had cancer cervix. Ovarian transposition was done for patients during surgery followed by external beam radiotherapy to the pelvis, hormonal assessment was done for the patients before and after exposure to radiation. Results: No intra-operative or post-operative morbidity was observed in patients treated by laparotomy. Patients were found to have regular menstrual cycles. Their FSH, LH and progesterone levels were within normal ranges. Conclusion: Proper transposition of ovaries during laparotomy away from the radiation field can preserve the ovarian function in patients with pelvic tumors.