We previously functionally characterized a novel marine microbial GDSL lipase MT6 and identified that the stereo-selectivity of MT6 was opposite to that of other common lipases in trans-esterification reactions.Herein...We previously functionally characterized a novel marine microbial GDSL lipase MT6 and identified that the stereo-selectivity of MT6 was opposite to that of other common lipases in trans-esterification reactions.Herein,we have investigated the use of MT6 in stereo-selective biocatalysis through direct hydrolysis reactions.Notably,the stereo-selectivity of MT6 was also demonstrated to be opposite to that of other common lipases in hydrolysis reactions.Parameters,including temperature,organic co-solvents,pH,ionic strength,catalyst loading,substrate concentration,and reaction time,affecting the enzymatic resolution of racemic 1-phenylethyl acetate were further investigated,with the e.e.of the final（S）-l-Phenylethanol product and the conversion being 97%and 28.5%,respectively,after process optimization.The lengths of side chains of 1-phenylethyl esters greatly affected the stereo-selectivity and conversion during kinetic resolutions.MT6 is a novel marine microbial GDSL lipase exhibiting opposite stereo-selectivities than other common lipases in both trans-esterification reactions and hydrolysis reactions.展开更多
Applying the consistent Riccati expansion method, the extended(2+1)-dimensional shallow water wave equation is proved consistent Riccati solvable and the exact interaction solutions including soliton-cnoidal wave solu...Applying the consistent Riccati expansion method, the extended(2+1)-dimensional shallow water wave equation is proved consistent Riccati solvable and the exact interaction solutions including soliton-cnoidal wave solutions,solitoff-typed solutions are obtained. With the help of the truncated Painlev′e expansion, the corresponding nonlocal symmetry is also given, and furthermore, the nonlocal symmetry is localized by prolonging the related enlarged system.展开更多
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences(XDA11030404),the Key Project from Chinese Academy of Sciences(KGZD-EW-606)the National Natural Science Foundation of China(21302199)Guangzhou Science and Technology Plan Projects(201510010012)
文摘We previously functionally characterized a novel marine microbial GDSL lipase MT6 and identified that the stereo-selectivity of MT6 was opposite to that of other common lipases in trans-esterification reactions.Herein,we have investigated the use of MT6 in stereo-selective biocatalysis through direct hydrolysis reactions.Notably,the stereo-selectivity of MT6 was also demonstrated to be opposite to that of other common lipases in hydrolysis reactions.Parameters,including temperature,organic co-solvents,pH,ionic strength,catalyst loading,substrate concentration,and reaction time,affecting the enzymatic resolution of racemic 1-phenylethyl acetate were further investigated,with the e.e.of the final（S）-l-Phenylethanol product and the conversion being 97%and 28.5%,respectively,after process optimization.The lengths of side chains of 1-phenylethyl esters greatly affected the stereo-selectivity and conversion during kinetic resolutions.MT6 is a novel marine microbial GDSL lipase exhibiting opposite stereo-selectivities than other common lipases in both trans-esterification reactions and hydrolysis reactions.
基金Supported by the National Natural Science Foundation of China under Grant Nos.11405103,11571008,51679132,11601321,and 11526137
文摘Applying the consistent Riccati expansion method, the extended(2+1)-dimensional shallow water wave equation is proved consistent Riccati solvable and the exact interaction solutions including soliton-cnoidal wave solutions,solitoff-typed solutions are obtained. With the help of the truncated Painlev′e expansion, the corresponding nonlocal symmetry is also given, and furthermore, the nonlocal symmetry is localized by prolonging the related enlarged system.
