To obtain the P8 protein of Rice gall dwarf virus (RGDV) with biological activity,its outer coat protein gene S8 was expressed in Spodoptera frugiperda (Sf9) insect cells using the baculovirus expression system.The S8...To obtain the P8 protein of Rice gall dwarf virus (RGDV) with biological activity,its outer coat protein gene S8 was expressed in Spodoptera frugiperda (Sf9) insect cells using the baculovirus expression system.The S8 gene was subcloned into the pFastBacTM1 vector,to produce the recombinant baculovirus transfer vector pFB-S8.After transformation,pFB-S8 was introduced into the competent cells (E.coli DH10Bac) containing a shuttle vector,Bacmid,generating the recombinant bacmid rbpFB-S8.After being infected by recombinant baculovirus rvpFB-S8 at different multiplicities of infection,Sf9 cells were collected at different times and analyzed by SDS-PAGE,Western blotting and immunofluorescence microscopy.The expression level of the P8 protein was highest between 48-72 h after transfection of Sf9 cells.Immunofluorescence microscopy showed that P8 protein of RGDV formed punctate structures in the cytoplasm of Sf9 cells.展开更多
This paper describes how aquatic insect diversity in major natural habitats of Agusan marsh relates with nearby ricefields to determine the interdependence between them for sustainable rice production through natural ...This paper describes how aquatic insect diversity in major natural habitats of Agusan marsh relates with nearby ricefields to determine the interdependence between them for sustainable rice production through natural pest control and for conservation of aquatic fauna in the marsh. Sampling for one year including two cropping seasons of rice production was conducted in various natural habitats of the marsh and in the adjoining rice fields. Both the natural habitats and rice fields were characterized in relation to determining habitat suitability for aquatic insects. The sedge-dominated swamp had the highest diversity among the natural habitats, while the fern-dominated swamp had the lowest. In the adjacent ricefields, diversity was similarly highest in the site near the sedge-dominated swamp, while the lowest was in the ricefields adjoining the Sago forest. Species composition of aquatic insects among the habitats differed between the wet and dry seasons. However, similarity patterns exist between natural habitats and rice fields. The closer similarity in species composition between natural habitats and ricefields indicates habitat connectivity which is an important consideration in planning and effective pest management and ecologically sound rice farming in the marsh.展开更多
基金supported by the National Science Foundation of China (30970135)The Key Project of Genetically Modified Organisms Breeding(2009ZX08009-044B)+1 种基金the Natural Science Foundation of Fujian Province of China (No.2006J0065)the Public-interest Scientific Institution Basal Research Fund of Fujian Province (2009R10029-3)
文摘To obtain the P8 protein of Rice gall dwarf virus (RGDV) with biological activity,its outer coat protein gene S8 was expressed in Spodoptera frugiperda (Sf9) insect cells using the baculovirus expression system.The S8 gene was subcloned into the pFastBacTM1 vector,to produce the recombinant baculovirus transfer vector pFB-S8.After transformation,pFB-S8 was introduced into the competent cells (E.coli DH10Bac) containing a shuttle vector,Bacmid,generating the recombinant bacmid rbpFB-S8.After being infected by recombinant baculovirus rvpFB-S8 at different multiplicities of infection,Sf9 cells were collected at different times and analyzed by SDS-PAGE,Western blotting and immunofluorescence microscopy.The expression level of the P8 protein was highest between 48-72 h after transfection of Sf9 cells.Immunofluorescence microscopy showed that P8 protein of RGDV formed punctate structures in the cytoplasm of Sf9 cells.
文摘This paper describes how aquatic insect diversity in major natural habitats of Agusan marsh relates with nearby ricefields to determine the interdependence between them for sustainable rice production through natural pest control and for conservation of aquatic fauna in the marsh. Sampling for one year including two cropping seasons of rice production was conducted in various natural habitats of the marsh and in the adjoining rice fields. Both the natural habitats and rice fields were characterized in relation to determining habitat suitability for aquatic insects. The sedge-dominated swamp had the highest diversity among the natural habitats, while the fern-dominated swamp had the lowest. In the adjacent ricefields, diversity was similarly highest in the site near the sedge-dominated swamp, while the lowest was in the ricefields adjoining the Sago forest. Species composition of aquatic insects among the habitats differed between the wet and dry seasons. However, similarity patterns exist between natural habitats and rice fields. The closer similarity in species composition between natural habitats and ricefields indicates habitat connectivity which is an important consideration in planning and effective pest management and ecologically sound rice farming in the marsh.
