[Objective] The cloning and transformation of rice OsOle1 gene were conducted in the research.[Method] OsOle1 gene was cloned by RT-PCR.The amplified OsOle1 was then ligased to pCAMBIA 1300 to construct GUS overexpres...[Objective] The cloning and transformation of rice OsOle1 gene were conducted in the research.[Method] OsOle1 gene was cloned by RT-PCR.The amplified OsOle1 was then ligased to pCAMBIA 1300 to construct GUS overexpression vector.Then the Agrobacterium-mediated method was used in rice callus transformation.[Result] The full-length of OsOle1 gene was 498 bp and it encoded 189 amino acids.The over-expression vector Ub：：OsOe1-GUS was prepared and transgenic plants were successfully obtained.[Conclusion] The transgenic lines laid the foundation for the function research of OsOle1.展开更多
[Objective] The aim was to clone the mitochondrial-related gene nad1 and produce transgenic rice plants with nad1.[Method] The total RNA was extracted from rice seedlings and reverse transcripted into cDNA.Then the ta...[Objective] The aim was to clone the mitochondrial-related gene nad1 and produce transgenic rice plants with nad1.[Method] The total RNA was extracted from rice seedlings and reverse transcripted into cDNA.Then the target gene nad1 was amplified by using the cDNA as template.The nad1 and Rf1b,a sequence of signal peptide of mitochondria,were linked to binary expression vector pCAMBIA1305.1.The recombinant plasmid was transformed into the callus by Agrobacterium-mediated approach.[Result] The target gene nad1 was 978 bp.The binary expression vector carrying nad1 and signal peptide of mitochondria was constructed successfully.In addition,a lot of transgenic plants were obtained.[Conclusion] The study will provide basis to investigate the effect of over-expression of nad1 on rice plant growth.展开更多
[Objective] This study aimed to clone the gene atp6 from rice mitochondria, construct the binary expression vector 35S ：： Rflb5＇ ：： atp6 and obtain transgenic plants with atp6. [Method] The special primers were d...[Objective] This study aimed to clone the gene atp6 from rice mitochondria, construct the binary expression vector 35S ：： Rflb5＇ ：： atp6 and obtain transgenic plants with atp6. [Method] The special primers were designed according to the sequence of target gene. With the TRIzol method the total RNA was extracted from rice seedlings and reverse transcripted to cDNA. The ORF of atp6 was amplified by PCR, and ligated to binary expression vector pCAMBIA1302 which contains se- quence of signal peptide from mitochondria （Rflb5＇）. The recombinant plasmid was then transformed into rice callus mediated by Agrobacterium. [Result] The binary expression vector 35S ：： Rflb5＇ ：： atp6 was constructed, and positive transgenic plants were obtained. [Conclusion] This study lays foundation for understanding influence of atp6 gene over-expression on rice growth.展开更多
文摘[Objective] The cloning and transformation of rice OsOle1 gene were conducted in the research.[Method] OsOle1 gene was cloned by RT-PCR.The amplified OsOle1 was then ligased to pCAMBIA 1300 to construct GUS overexpression vector.Then the Agrobacterium-mediated method was used in rice callus transformation.[Result] The full-length of OsOle1 gene was 498 bp and it encoded 189 amino acids.The over-expression vector Ub：：OsOe1-GUS was prepared and transgenic plants were successfully obtained.[Conclusion] The transgenic lines laid the foundation for the function research of OsOle1.
基金Supported by the National Natural Science Foundation of China(30871318)~~
文摘[Objective] The aim was to clone the mitochondrial-related gene nad1 and produce transgenic rice plants with nad1.[Method] The total RNA was extracted from rice seedlings and reverse transcripted into cDNA.Then the target gene nad1 was amplified by using the cDNA as template.The nad1 and Rf1b,a sequence of signal peptide of mitochondria,were linked to binary expression vector pCAMBIA1305.1.The recombinant plasmid was transformed into the callus by Agrobacterium-mediated approach.[Result] The target gene nad1 was 978 bp.The binary expression vector carrying nad1 and signal peptide of mitochondria was constructed successfully.In addition,a lot of transgenic plants were obtained.[Conclusion] The study will provide basis to investigate the effect of over-expression of nad1 on rice plant growth.
基金Supported by National Natural Science Foundation of China(3117022631170296)~~
文摘[Objective] This study aimed to clone the gene atp6 from rice mitochondria, construct the binary expression vector 35S ：： Rflb5＇ ：： atp6 and obtain transgenic plants with atp6. [Method] The special primers were designed according to the sequence of target gene. With the TRIzol method the total RNA was extracted from rice seedlings and reverse transcripted to cDNA. The ORF of atp6 was amplified by PCR, and ligated to binary expression vector pCAMBIA1302 which contains se- quence of signal peptide from mitochondria （Rflb5＇）. The recombinant plasmid was then transformed into rice callus mediated by Agrobacterium. [Result] The binary expression vector 35S ：： Rflb5＇ ：： atp6 was constructed, and positive transgenic plants were obtained. [Conclusion] This study lays foundation for understanding influence of atp6 gene over-expression on rice growth.
