The present study evaluated the effect of dl-3-n-butylphthalide(NBP) ,a novel brain protective agent, on brain edema in rats following focal ischemia. Edema was induced by occluding the right middle cerebral artery (M...The present study evaluated the effect of dl-3-n-butylphthalide(NBP) ,a novel brain protective agent, on brain edema in rats following focal ischemia. Edema was induced by occluding the right middle cerebral artery (MCAO).producing permanent focal ischemia in the right cerebral hemisphere,which developed ip-silateral brain edema reproducibly. Edema was assessed 24 h after MCA occlusion by determining the brain water content from wet and dry weight measurements,and the sodium,potassium concentrations with ion-selective electrodes. In this model,NBP at the dose of 80,160 and 240 mg/kg po 15 min after MCAO prevented from brain edema in a dose-dependent manner. A significant reduction of sodium content and an increase in potassium level were observed in all drug-treated groups. It showed that NBP strongly attenuated brain water entry,sodium accumulation and potassium loss. Nimodipine treatment(5mg/kg sc) also reduced brain edema (P<0. 05). The results suggest that a strong anti-edema activity of NBP may play an important role to contribute to the treatment of ischemic damage.展开更多
Objective: To investigate the effects of magnesium sulfate on traumatic brain edema and explore its possible mechanism. Methods: Forty-eight Sprague-Dawley (SD) rats were randomly divided into three groups: Control, T...Objective: To investigate the effects of magnesium sulfate on traumatic brain edema and explore its possible mechanism. Methods: Forty-eight Sprague-Dawley (SD) rats were randomly divided into three groups: Control, Trauma and Treatment groups. In Treatment group, magnesium sulfate was intraperitoneally administered immediately after the induction of brain trauma. At 24 h after trauma, total tissue water content and Na +, K +, Ca 2+, Mg 2+ contents were measured. Permeability of blood-brain barrier (BBB) was assessed quantitatively by Evans Blue (EB) dye technique. The pathological changes were also studied. Results: Water, Na +, Ca 2+ and EB contents in Treatment group were significantly lower than those in Trauma group (P< 0.05). Results of light microscopy and electron microscopy confirmed that magnesium sulfate can attenuate traumatic brain injury and relieve BBB injury. Conclusions: Treatment with MgSO 4 in the early stage can attenuate traumatic brain edema and prevent BBB injury.展开更多
文摘目的:复制家兔急性肺水肿动物模型,从中筛选出合适的动物模型,并探讨其产生的机制。方法:采用家兔耳缘静脉分别注入1∶1 000肾上腺素的方法,建立家兔实验性肺水肿模型。通过观察动物的一般情况、肺系数,确定适合的家兔急性肺水肿动物模型的方法。结果:输入肾上腺素后,各实验组平均动脉压(MAP)、呼吸(R)、心率(P)与空白组比较明显升高,差异有统计学意义(P<0.05),但各实验组之间比较差异无统计学意义(P>0.05);湿啰音在生理盐水注入量为100~200 m L/kg、100~200滴/min的滴率、注入肾上腺素0.10~0.40 mg/kg的实验组均出现,且实验组之间产生肺水肿的时间和肺系数比较差异无统计学意义(P>0.05)。结论:较小剂量使用肾上腺素亦可引发家免肺水肿,其病理变化明显,同时实验过程有所延长,便于观察家兔在肺水肿形成过程中的症状和体征变化。
文摘The present study evaluated the effect of dl-3-n-butylphthalide(NBP) ,a novel brain protective agent, on brain edema in rats following focal ischemia. Edema was induced by occluding the right middle cerebral artery (MCAO).producing permanent focal ischemia in the right cerebral hemisphere,which developed ip-silateral brain edema reproducibly. Edema was assessed 24 h after MCA occlusion by determining the brain water content from wet and dry weight measurements,and the sodium,potassium concentrations with ion-selective electrodes. In this model,NBP at the dose of 80,160 and 240 mg/kg po 15 min after MCAO prevented from brain edema in a dose-dependent manner. A significant reduction of sodium content and an increase in potassium level were observed in all drug-treated groups. It showed that NBP strongly attenuated brain water entry,sodium accumulation and potassium loss. Nimodipine treatment(5mg/kg sc) also reduced brain edema (P<0. 05). The results suggest that a strong anti-edema activity of NBP may play an important role to contribute to the treatment of ischemic damage.
文摘Objective: To investigate the effects of magnesium sulfate on traumatic brain edema and explore its possible mechanism. Methods: Forty-eight Sprague-Dawley (SD) rats were randomly divided into three groups: Control, Trauma and Treatment groups. In Treatment group, magnesium sulfate was intraperitoneally administered immediately after the induction of brain trauma. At 24 h after trauma, total tissue water content and Na +, K +, Ca 2+, Mg 2+ contents were measured. Permeability of blood-brain barrier (BBB) was assessed quantitatively by Evans Blue (EB) dye technique. The pathological changes were also studied. Results: Water, Na +, Ca 2+ and EB contents in Treatment group were significantly lower than those in Trauma group (P< 0.05). Results of light microscopy and electron microscopy confirmed that magnesium sulfate can attenuate traumatic brain injury and relieve BBB injury. Conclusions: Treatment with MgSO 4 in the early stage can attenuate traumatic brain edema and prevent BBB injury.
