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复方SZ滴眼液对晶状体上皮细胞凋亡的抑制作用及其信号转导机制 被引量:5
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作者 黄秀榕 祁明信 +2 位作者 陈义 汪朝阳 李坤鹏 《中国病理生理杂志》 CAS CSCD 北大核心 2006年第10期2044-2048,共5页
目的:探讨复方SZ滴眼液(Co-SZ)抑制H2O2诱导的晶状体上皮细胞(LEC)凋亡的作用及信号转导机制。为将Co-SZ作为防治白内障的有效药物提供实验依据。方法:(1)将H2O2与Co-SZ和SD大鼠晶状体共同孵育后:TUNEL法检测LEC凋亡率。透射电镜观察LE... 目的:探讨复方SZ滴眼液(Co-SZ)抑制H2O2诱导的晶状体上皮细胞(LEC)凋亡的作用及信号转导机制。为将Co-SZ作为防治白内障的有效药物提供实验依据。方法:(1)将H2O2与Co-SZ和SD大鼠晶状体共同孵育后:TUNEL法检测LEC凋亡率。透射电镜观察LEC超微结构改变及凋亡形成。(2)H2O2与Co-SZ和体外培养的牛LEC共同孵育后,四甲基偶氮唑蓝法(MTT)检测不同浓度的Co-SZ抑制LEC凋亡率。流式细胞仪(FCM)检测LEC细胞核内DNA含量。荧光分光光度法检测LEC内游离Ca2+浓度。放射免疫分析法检测LEC内环化腺苷酸(cAMP)和环化鸟苷酸(cGMP)浓度。结果:TUNEL法检测Co-SZ组LEC凋亡率显著低于H2O2组。Co-SZ组LEC超微结构变化也显著轻于H2O2组。MTT检测Co-SZ组细胞活性明显高于H2O2并具有剂量依赖关系。Co-SZ组LEC核内DNA含量增加。Co-SZ使LEC内游离Ca2+、cAMP降低、cGMP升高。结论:Co-SZ能有效抑制H2O2诱导的LEC发生的凋亡。Co-SZ抑制LEC凋亡的机制可能是通过抑制LEC核内DNA降解,并通过抑制细胞内游离钙离子浓度升高、阻断Ca2+-钙调蛋白依赖性蛋白激酶途径和Ca2+-蛋白激酶C信号转导途径。 展开更多
关键词 滴眼液 水蜂 晶体上皮细胞 细胞凋亡 信号转导
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Water Soluble Propolis and Royal Jelly Enhance the Antimicrobial Activity of Honeys and Promote the Growth of Human Macrophage Cell Line 被引量:1
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作者 Bratko Filipic Lidija Gradisnik +7 位作者 Eva Ruzic-Sabljic Brabara Trtnik Adriana Pereyra Domen Jaklic Rok Kopinc Jana Potokar Almin Puzic Hrvoje Mazija 《Journal of Agricultural Science and Technology(B)》 2016年第1期35-47,共13页
Due to the overuse and misuse of antibiotic, an increase in antibiotic resistance of pathogenic bacteria is evolving. Attention should be focused on natural alternatives to antibiotics, like propolis, royal jelly (R ... Due to the overuse and misuse of antibiotic, an increase in antibiotic resistance of pathogenic bacteria is evolving. Attention should be focused on natural alternatives to antibiotics, like propolis, royal jelly (R J) and honeys. They all have strong antibacterial properties due to the active substances they contain. This study investigated the effect of combination of water soluble propolis (WSP) Greitl20 or fresh royal jelly (F-RJ) (MiZigoj) and Forest honeys as antibacterial against Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Acinetobacter baumanii, Staphylococcus aureus, methicillin resistant Staphylococcus aureus (MRSA), Streptococcus pyogenes, Streptococcus agalactiae and Candida albicans. These substances are also cell growth promoters for human macrophage (TLT) cell line. WSP Greitl20, F-RJ (M) and different Forest honeys were prepared in saline as 10% solutions. The antimicrobial activity was expressed as the minimal inhibitory concentration (MIC) in mg/mL. The growth promotion activity was measured at optical density (OD) 595 nm. The combination ofWSP Greitl20 with different Forest honeys is better than F-RJ (M) in same combination with different Forest honeys. The best antibacterial/antifungal activity was found with the combination of 10% WSP Greit 120 in the Forest honey (1:10) from Italy or Spain. When measuring the growth promoting activity of TLT cell line, the best activity was detected at the combination of 10% WSP Greitl20 in the Forest honey from Italy (GI3 = 0.796 ± 0.014 and GI5 = 1.133± 0.022). Antimicrobial and growth promoting activities are correlated and WSP-dependent. 展开更多
关键词 PROPOLIS royal jelly Forest honey antimicrobial activity human macrophage cell line cell growth promoting activity.
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Main Rice Pests Control with Trichogramma japonicum
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作者 LIU Yang YUAN Qiu-sheng +3 位作者 ZHANG Yu-zhu FANG Bao-hua KUANG Wei ZHU Guo-qi 《Agricultural Science & Technology》 CAS 2018年第1期28-32,共5页
This study used Trichogramma japonicumto to control Chilo suppressalis (Walker) and Cnaphalocrocis medinalis(Guenée), and tested the effects of different control methods on main rice pests, natural enemies an... This study used Trichogramma japonicumto to control Chilo suppressalis (Walker) and Cnaphalocrocis medinalis(Guenée), and tested the effects of different control methods on main rice pests, natural enemies and rice yield. The results revealed that the parasitism of Trichogramma japonicum in the eggs of Chilo suppressalis (Walker) and Cnaphalocrocis medinalis (Guenée) reached 60.3% and 57.1%, and the control efficacy was up to 70.8 % and 91.99%, respectively. The control efficacy for Cnaphalocrocis medinalis (Guenée) was much better. This biological control method could be equivalent to chemical control method in the control effects on main pests in rice. The yield under the biological control was 26.5% higher than that of the control group, and the biological control had a signifcant increase in the number of natural enemies such as spider, up to 400 per 100 plants. Therefore, the sustainable prevention and control were achieved in the presentence with natural enemy organism. 展开更多
关键词 Trichogramma japonicum Biological control RICE Chilo suppressalis (walker) Cnaphalocrocis medinalis (Guenée)
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Unconventional Landau Levels of Ultracold Fermionic Atoms on Two-Dimensional Honeycomb Lattice
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作者 HOU Jing-Min 《Communications in Theoretical Physics》 SCIE CAS CSCD 2009年第8期251-254,共4页
We investigate the unconventional Landau levels of ultracold fermionic atoms on the two-dimensionalhoneycomb optical lattice subjected to an effective magnetic field,which is created with optical means.In the presence... We investigate the unconventional Landau levels of ultracold fermionic atoms on the two-dimensionalhoneycomb optical lattice subjected to an effective magnetic field,which is created with optical means.In the presenceof the effective magnetic field,the energy spectrum of the unconventional Landau levels is calculated.Furthermore,wepropose to detect the unconventional Landau levels with Bragg scattering techniques. 展开更多
关键词 ultracold atoms honeycomb optical lattice unconventional Landau levels
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The recent disciplinal progresses of agricultural entomology in China
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作者 Guo Yuyuan Liang Gemei 《Engineering Sciences》 EI 2009年第3期2-15,共14页
In this paper, four recent advances and achievements of China in agricultural insect research, namely, on the genome of silkworm (Bombyx mori Linnaeus), on the geographical differentiation and regional migration of co... In this paper, four recent advances and achievements of China in agricultural insect research, namely, on the genome of silkworm (Bombyx mori Linnaeus), on the geographical differentiation and regional migration of cotton bollworm (Helicoverpa armigera (Hübner)), on the standardized monitoring techniques for safety of honey bee (Apis mellifera Linnaeus) products, and on the virus transmission property of small brown planthopper (Laodelphax striatellus (Fallén)) as well as the interactions between vector and rice stripe virus (RSV), were reported. All of these researches are very important for controlling agricultural insect pests and the diseases they transmit, accelerating the molecular biological research of silkworm, and promoting the international trade of honey bee products. Most of these achievements mentioned above have got the national, provincial, ministerial or municipal awards on science and technology. 展开更多
关键词 recent advances and achievements of China agricultural insect research control agricultural insect pests and diseases molecular biology research international trade
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Honeycomb-structured solid acid catalysts fabricated via the swelling-induced self-assembly of acidic poly(ionic liquid)s for highly efficient hydrolysis reactions 被引量:2
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作者 Bihua Chen Tong Ding +6 位作者 Xi Deng Xin Wang Dawei Zhang Sanguan Ma Yongya Zhang Bing Ni Guohua Gao 《Chinese Journal of Catalysis》 SCIE EI CAS CSCD 2021年第2期297-309,共13页
The development of heterogeneous acid catalysts with higher activity than homogeneous acid catalysts is critical and still challenging.In this study,acidic poly(ionic liquid)s with swelling ability(SAPILs)were designe... The development of heterogeneous acid catalysts with higher activity than homogeneous acid catalysts is critical and still challenging.In this study,acidic poly(ionic liquid)s with swelling ability(SAPILs)were designed and synthesized via the free radical copolymerization of ionic liquid monomers,sodium p-styrenesulfonate,and crosslinkers,followed by acidification.The 31P nuclear magnetic resonance chemical shifts of adsorbed trimethylphosphine oxide indicated that the synthesized SAPILs presented moderate and single acid strength.The thermogravimetric analysis results in the temperature range of 300–345°C revealed that the synthesized SAPILs were more stable than the commercial resin Amberlite IR-120(H)(245°C).Cryogenic scanning electron microscopy testing demonstrated that SAPILs presented unique three-dimensional(3D)honeycomb structure in water,which was ascribed to the swelling-induced self-assembly of the molecules.Moreover,we used SAPILs with micron-sized honeycomb structure in water as catalysts for the hydrolysis of cyclohexyl acetate to cyclohexanol,and determined that their catalytic activity was much higher than that of homogeneous acid catalysts.The equilibrium concentrations of all reaction components inside and outside the synthesized SAPILs were quantitatively analyzed using a series of simulated reaction mixtures.Depending on the reaction mixture,the concentration of cyclohexyl acetate inside SAPIL-1 was 7.5–23.3 times higher than that outside of it,which suggested the high enrichment ability of SAPILs for cyclohexyl acetate.The excellent catalytic performance of SAPILs was attributed to their 3D honeycomb structure in water and high enrichment ability for cyclohexyl acetate,which opened up new avenues for designing highly efficient heterogeneous acid catalysts that could eventually replace conventional homogeneous acid catalysts. 展开更多
关键词 Heterogeneous acid catalyst Acidic poly(ionic liquid) SWELLING 3D honeycomb structure Enrichment Hydrolysis HYDRATION
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