AIM:To investigate the contribution of periostin in nicotine-promoted gastric cancer cell proliferation,survival,invasion,drug resistance,and epithelial-mesenchymal transition(EMT).METHODS:Gastric cancer cells were tr...AIM:To investigate the contribution of periostin in nicotine-promoted gastric cancer cell proliferation,survival,invasion,drug resistance,and epithelial-mesenchymal transition(EMT).METHODS:Gastric cancer cells were treated with nicotine and periostin protein expression was determined by immunoblotting.Periostin mRNA in gastric cancer cells was silenced using small interfering RNA(siRNA) techniques and periostin gene expression was evaluated by quantitative reverse transcription-polymerase chain reaction.Gastric cancer cells transfected with control or periostin siRNA plasmid were compared in terms of cell proliferation using the methylthiazolyldiphenyl-tetrazolium bromide assay.Cell apoptosis was compared using annexin V-fluoresceine isothiocyanate and propidium iodine double staining.Tumor invasion was determined using the Boyden chamber invasion assay,and the EMT marker Snail expression was evaluated by immunoblotting.RESULTS:Nicotine upregulated periostin in gastric cancer cells through a COX-2 dependent pathway,which was blocked by the COX-2-specific inhibitor NS398.Periostin mRNA expression was decreased by ~87.2% by siRNA in gastric cancer cells,and stable periostinsilenced cells were obtained by G418 screening.Periostin-silenced gastric cancer cells exhibited reduced cell proliferation,elevated sensitivity to chemotherapy with 5-fluorouracil,and decreased cell invasion and Snail expression(P < 0.05).CONCLUSION:Periostin is a nicotine target gene in gastric cancer and plays a role in gastric cancer cell growth,invasion,drug resistance,and EMT facilitated by nicotine.展开更多
This study examined the impact of the preceding boreal summer(June–August) North Atlantic Oscillation(NAO) on early autumn(September) rainfall over Central China(RCC). The results show that a significant positive cor...This study examined the impact of the preceding boreal summer(June–August) North Atlantic Oscillation(NAO) on early autumn(September) rainfall over Central China(RCC). The results show that a significant positive correlation exists between the preceding summer NAO and the early autumn RCC on the interannual timescale. In order to understand the physical mechanism between them, the role of ocean was investigated. It was found that the strong summer NAO can induce a tripole sea surface temperature anomaly(SSTA) in the North Atlantic; this SSTA pattern can persist until early autumn. The diagnostic analysis showed that the tripole SSTA pattern excites a downstream Atlantic-Eurasian(AEA) teleconnection, which contributes to an increase in RCC. The circulation anomalies related to SSTA caused by the weak NAO are opposite, so the RCC is less than normal. The results imply that the preceding summer NAO may be regarded as a forecast factor for the early autumn RCC.展开更多
Heavy metal pollutants are a worldwide concern due to slow decomposition, biocondensation, and negative effects on human health. We investigated seasonal and spatial variations of the five heavy metals and evaluated t...Heavy metal pollutants are a worldwide concern due to slow decomposition, biocondensation, and negative effects on human health. We investigated seasonal and spatial variations of the five heavy metals and evaluated their health risk in the Liaohe River, Northeast China. A total of 324 surface water samples collected from 2009 to 2010 were analyzed. Levels(high to low) of heavy metals in the Liaohe River were: zinc(Zn) > chromium(Cr) > copper(Cu) > cadmium(Cd) > mercury(Hg). Spatial and seasonal changes impacting concentrations of Cu and Zn were significant, but not significant for Cr, Cd and Hg. The highest concentrations of heavy metals were: Hg at Liuheqiao, Cu at Fudedian, Zn at Tongjiangkou, Cr at Mahushan, and Cd at Shenglitang. The highest concentrations of Hg and Cr were found in the wet period, Cu and Cd in the level period, and Zn in the dry period. The surface water of a tributary was an important accumulation site for heavy metals. Health risks from carcinogens and non-carcinogens increased from upstream to downstream in the mainstream of the Liaohe River. The total health risk for one person in the Liaohe River exceeded acceptable levels. The total health risk was the greatest during the wet period and least in the dry period. Among the five heavy metals in the Liaohe River, Cr posed the greatest single health risk.展开更多
Wano, spoken by about 7,000 native speakers, is a Papuan language of Trans-New Guinea Phylum, Dani-Kwerba Stock, which is found in the interior of Papua of the regency of Puncak Jaya. The language is closely related t...Wano, spoken by about 7,000 native speakers, is a Papuan language of Trans-New Guinea Phylum, Dani-Kwerba Stock, which is found in the interior of Papua of the regency of Puncak Jaya. The language is closely related to Dani, Walak, and Nggem. It is an SOV language typology that has a complex morphological system. Four spatial dimensions are morphosyntactically coded in elevative deixis, which are steepness/non-steepness distinction, proximity/distality distinction, adverbial/attributive expressions, and vertical/horizontal plane. This paper discusses the grammatical operation of a set of two-term system: ei "up" and ou "down" that serves as the basic forms for the elevational deixis in Wano.展开更多
Water mist technology provides efficient firefighting performance while there is still room for improvement. So varieties of additives have been studied in recent years both at home and abroad. The self-made additives...Water mist technology provides efficient firefighting performance while there is still room for improvement. So varieties of additives have been studied in recent years both at home and abroad. The self-made additives are used to compare the firefighting performance of diesel and heptane fire in open space. By adjusting the concentration of substance in the additives and conducting the experiment under the pressure of 0.3 MPa,0.5 MPa and 0.7 MPa,extinguish time and temperature are measured in the experiment. Through the experiments using different fuels,it can be found when the fuel is heptane that has a lower ignition point and a higher evaporation rate, the water mist additive can still significantly improve the firefighting performance. According to the data based on different concentrations of fluorinated surfactants,we find that fluorinated surfactants are the main substance to improve the performances by changing physical property of water mist. Optimal proportion of the additives for firefighting performance is found by experiment results.展开更多
In this study, X-ray diffraction (XRD), Fourier transform infrared spectrometer (FTIR) together with Scanning probe microscopy (SPM) were used to characterize the structure and morphology of the complexes, where...In this study, X-ray diffraction (XRD), Fourier transform infrared spectrometer (FTIR) together with Scanning probe microscopy (SPM) were used to characterize the structure and morphology of the complexes, where the hydrobiotites (Xinfiang) were modified by single-chain surfactants octyltrimethylammonium bromide (OTMA) and octadecyltrimethylammonium bromide (ODTMA). XRD patterns showed that the structure of complexes was significantly influenced by the surfactant concentration and the alkyl chain length, because obvious changes took place in the basal spacing. Furthermore, according to the XRD results, several arrangements of surfactant molecules within the hydrobiotite interlayer space were deduced. The FTIR spectrum indicated that the surfactant contents in complexes dramatically increased with the alkyl chain length. The SPM micrographs demonstrated that the surfaces of complexes prepared at lower surfactant concentration were relatively flat compared with that prepared at higher concentration, while those with higher surfactant concentration had much steeper surface due to the alkyl chain length. It was concluded that structure and morphology of surfactant/hydrobiotite complexes depend not only on the surfactant concentration, but also strongly on the surfactant species.展开更多
Objective: To study the effect and mechanism of aspirin alone or combined with cisplatin (DDP) on human cervical carcinoma HeLa cells. Methods: HeLa cells were treated by different concentrations of aspirin, DDP a...Objective: To study the effect and mechanism of aspirin alone or combined with cisplatin (DDP) on human cervical carcinoma HeLa cells. Methods: HeLa cells were treated by different concentrations of aspirin, DDP alone or both. The inhibitory effect on cell growth was analyzed by MTT and colony-forming assay. Cell apoptosis was measured with flow cytometry. The mRNA levels ofBcl-2, Bax and NF-κB(P65) were studied by RT-PCR. Results: MTT assay showed that aspirin inhibited HeLa cell proliferation in a time-and dose-dependant maoner. Aspirin decreased clone numbers in colony formation assay. Aspirin also induced apoptosis of HeLa cells in a dose- and time-dependent manner as detected by flow cytometry. The inhibition effects on proliferation, colony formation and apoptosis were significantly enhanced when cells were treated with both aspirin and DDP. RT-PCR demonstrated that aspirin decreased the transcription of Bcl-2 and NF-κB, and increased expression of Bax gene. Conclusion: Aspirin can induce apoptosis in HeLa cells. Combination of aspirin and DDP displays a synergistic effect. The possible mechanism might be that aspirin downregulates the mRNA levels of Bcl-2 and NF-κB gene and upregulates the expression of Bax.展开更多
As the most import fault of the Late Pleistocene in the Lhasa area,the Nalinlaka fault is a left-lateral thrust fault,striking NWW,dipping SSW with a high dip angle,and extending over 33km.According to studies on the ...As the most import fault of the Late Pleistocene in the Lhasa area,the Nalinlaka fault is a left-lateral thrust fault,striking NWW,dipping SSW with a high dip angle,and extending over 33km.According to studies on the latest strata on the Nalinlaka fault zone,this fault zone has been obviously active since the Late Pleistocene and the movement left behind some geomorphologic phenomena on the earth's surface,especially at the sites of the gully west of Cijiaolin and around Xiecun village.For example,some rivers,ridges and terraces are displaced,forming beheaded gullies and fault escarps.The horizontal displacements since the Late Pleistocene at the above two places are 54m ~ 87m,20m ~ 67m,respectively.Based on studies on the 4 trenches along the fault using the progressive constraining method,we conclude that there might have been 5 paleoearthquake events along the Nalinlaka fault since 70ka B.P.,the ages of each paleoearthquake are 68.53,54.40,< 41.23,21.96,9.86ka B.P.,and the average recurrence interval is 14.67ka.Because of the limits of trenches and earthquake events exposed by each trench,no single trench completely revealed all 5 events.There may therefore be some errors in determining the upper and lower limits of some events in this article.展开更多
Regarding the lack of cold source for underground cooling systems from either mine inflow or return air, field experiments were taken in a high temperature deep coal mine with abundant cold source from surface water. ...Regarding the lack of cold source for underground cooling systems from either mine inflow or return air, field experiments were taken in a high temperature deep coal mine with abundant cold source from surface water. Taking Sanhejian coal mine as an example, this paper introduced the technology scheme of heat disaster governance using surface water cold source. The paper presents the basics of this field experiment at the beginning, following by the design and site layout of the cooling system including the analysis and calculation of cold source. Numerical calculation method is also applied based on the operation parameters to simulate the influence to the surface river ecosystem. The results suggest that the temperature of surface water shall be lower than 34 ℃ after heat exchange, and when more cooling capacities are needed in the future, increasing the water flow is more favorable than increasing the cooling range of water, which is better for the ecological environment protection,展开更多
The reuse of wastewater for the irrigation of farmlands is gaining popularity, and the nutrient leaching associated with wastewater irrigation is becoming a matter of concern. The variability of nitrogen and phosphoru...The reuse of wastewater for the irrigation of farmlands is gaining popularity, and the nutrient leaching associated with wastewater irrigation is becoming a matter of concern. The variability of nitrogen and phosphorus frac- tions in wastewater-irrigated soil was investigated in both horizontal and vertical directions in Kongjiaxiang, Tongliao, Inner Mongolia, China. The results showed that wastewater irrigation resulted in the concentrations of available N and P being 40.36% and 66.49% higher, respectively, than those with groundwater irrigation. Different forms of N and P exhibited significantly different distribution pattems. Higher concentrations of NO~ and total available N, as well as of those of Ca-P and total available P were observed near wastewater irrigation channels. Ca-P has a spatial distribu- tion pattern similar to that of available P. The concentrations of NO3 and NH4 were the highest in top soil and de- creased with depth. The complex interactions between nitrate leaching and nitrogen transformation processes (e.g., nitrification, denitrification, and mineralization) determined the vertical profile of NO3. The significant amount of NH~ loss inhibited its deep seepage. The Ca-bound compound contained more P than other inorganic fractions as a result of high Ca levels throughout the soil profile. The differences in the concentrations of Ca-bound P at different depths could be due to the upward flux or translocation of Ca from subsurface to surface soil and the sequestration of P.展开更多
The freezing and melting process of a small water droplet on a superhydrophobic cold surface was investigated using the Laser Induced Fluorescence(LIF)technique.The superhydrophobic surface was prepared using a sol-ge...The freezing and melting process of a small water droplet on a superhydrophobic cold surface was investigated using the Laser Induced Fluorescence(LIF)technique.The superhydrophobic surface was prepared using a sol-gel method on a red copper test plate.From the obtained fluorescence images,the phase transition characteristics during the freezing and melting process of a water droplet were clearly observed.It was found that,at the beginning of the droplet freezing process,liquid water turned into ice at a very fast rate.Such phase transition process decreased gradually with time and the volume of frozen ice approached a constant value at the end of the icing process.In addition,the freezing time was found to reduce with the decrease of the test plate temperature.Besides,when the test plate temperature is relatively high,the effect of droplet volume on the freezing time is very significant.Over all,we provide some tentative insights into the microphysical process related to the icing and melting process of water droplets.展开更多
Water-repellent(WR) soil greatly influences infiltration behavior. This research determined the impacts of WR levels of silt loam soil layer during infiltration. Three column scenarios were utilized, including homogen...Water-repellent(WR) soil greatly influences infiltration behavior. This research determined the impacts of WR levels of silt loam soil layer during infiltration. Three column scenarios were utilized, including homogeneous wettable silt loam or sand, silt loam over sand(silt loam/sand), and sand over silt loam(sand/silt loam). A 5-cm thick silt loam soil layer was placed either at the soil surface or 5 cm below the soil surface. The silt loam soil used had been treated to produce different WR levels, wettable, slightly WR, strongly WR, and severely WR. As the WR level increased from wettable to severely WR, the cumulative infiltration decreased. Traditional wetting front-related equations did not adequately describe the infiltration rate and time relationships for layered WR soils. The Kostiakov equation provided a good fit for the first infiltration stage. Average infiltration rates for wettable, slightly WR, strongly WR, and severely WR during the 2 nd infiltration stage were 0.126, 0.021, 0.002, and 0.001 mm min^(-1) for the silt loam/sand scenario,respectively, and 0.112, 0.003, 0.002, and 0.000 5 mm min^(-1) for the sand/silt loam scenario, respectively. Pseudo-saturation phenomena occurred when visually examining the wetting fronts and from the apparent changes in water content(?θ_(AP)) at the slightly WR,strongly WR, and severely WR levels for the silt loam/sand scenario. Much larger ?θAPvalues indicated the possible existence of finger flow. Delayed water penetration into the surface soil for the strongly WR level in the silt loam/sand scenario suggested negative water heads with infiltration times longer than 10 min. The silt loam/sand soil layers produced sharp transition zones of water content. The WR level of the silt loam soil layer had greater effects on infiltration than the layer position in the column.展开更多
Land surface water(LSW) is one of the most important resources for human survival and development, and it is also a main component of global water recycling. A full understanding of the spatial distribution of land su...Land surface water(LSW) is one of the most important resources for human survival and development, and it is also a main component of global water recycling. A full understanding of the spatial distribution of land surface water and a continuous measuring of its dynamics can support to diagnose the global ecosystem and environment. Based on the Global Land 30-water 2000 and Global Land 30-water 2010 products, this research analyzed the spatial distribution pattern and temporal fluctuation of land surface water under scale-levels of global, latitude and longitude, continents, and climate zones. The Global Land 30-water products were corrected the temporal inconsistency of original remotely sensed data using MODIS time-series data, and then calculated the indices such as water area, water ration and coefficient of spatial variation for further analysis. Results show that total water area of land surface is about 3.68 million km2(2010), and occupies 2.73% of land area. The spatial distribution of land surface water is extremely uneven and is gathered mainly in mid- to high-latitude area of the Northern Hemisphere and tropic area. The comparison of water ratio between 2000 and 2010 indicates the overall fluctuation is small but spatially differentiated. The Global Land 30-water products and the statistics provided the fundamental information for analyzing the spatial distribution pattern and temporal fluctuation of land surface water and diagnosing the global ecosystem and environment.展开更多
This paper describes the NiiMi process designed to treat landscape water. The main aim of the research was to investigate the feasibility of NiiMi for removing organic and nutriment materials from landscape water. Dur...This paper describes the NiiMi process designed to treat landscape water. The main aim of the research was to investigate the feasibility of NiiMi for removing organic and nutriment materials from landscape water. During the batch-scale NiiMi operation, the removal rates of color ranged from 66.7%o-80% , of turbidity from 31.7%-89.3%o, of chemical oxygen demand (COD) from 7%-36.5%, of total phosphor (TP) from 43%-84.2%, of soluble phosphate from 42.9%-100%, of total nitrogen (TN) from 4.2%-46.7%, and of NH4^+-N from 39.3%-100% at the hydraulic loading of 0.2 m^3/(m^2·d). Results showed that the removal efficiencies of COD, TP, soluble phosphate and TN decreased with the decline in the temperature. The NiiMi process had a strong shock loading ability for the removal of the organics, turbidity, TP, soluble phosphate, TN and NH4^+-N. Three sodium chloride tracer studies were conducted, labeled as TS 1, TS2, and TS3, respectively. The mean hydraulic retention times (mean HRTs) were 31 h and 28 h for TSI and TS2, respectively, indicating the occurrence of a dead zone volume of 12% and 20% for TS 1 and TS2, respectively. TS 1 and TS2 displayed the occurrence of short-circuiting in the NiiMi system. The comparison results between TS1 and TS2 were further confirmed in the values obtained for some indicators, such as volumetric efficiency (e), short-circuiting (S), hydraulic efficiency (2) and number of continuously stirred tank reactors (N).展开更多
基金Supported by Department of Pathology,Division of Basic Medicine,Central South University Xiangya School of Medicine
文摘AIM:To investigate the contribution of periostin in nicotine-promoted gastric cancer cell proliferation,survival,invasion,drug resistance,and epithelial-mesenchymal transition(EMT).METHODS:Gastric cancer cells were treated with nicotine and periostin protein expression was determined by immunoblotting.Periostin mRNA in gastric cancer cells was silenced using small interfering RNA(siRNA) techniques and periostin gene expression was evaluated by quantitative reverse transcription-polymerase chain reaction.Gastric cancer cells transfected with control or periostin siRNA plasmid were compared in terms of cell proliferation using the methylthiazolyldiphenyl-tetrazolium bromide assay.Cell apoptosis was compared using annexin V-fluoresceine isothiocyanate and propidium iodine double staining.Tumor invasion was determined using the Boyden chamber invasion assay,and the EMT marker Snail expression was evaluated by immunoblotting.RESULTS:Nicotine upregulated periostin in gastric cancer cells through a COX-2 dependent pathway,which was blocked by the COX-2-specific inhibitor NS398.Periostin mRNA expression was decreased by ~87.2% by siRNA in gastric cancer cells,and stable periostinsilenced cells were obtained by G418 screening.Periostin-silenced gastric cancer cells exhibited reduced cell proliferation,elevated sensitivity to chemotherapy with 5-fluorouracil,and decreased cell invasion and Snail expression(P < 0.05).CONCLUSION:Periostin is a nicotine target gene in gastric cancer and plays a role in gastric cancer cell growth,invasion,drug resistance,and EMT facilitated by nicotine.
基金supported jointly by the National Basic Research Program of China(973 program,Grant No.2013CB340203)the National Natural Science Foundation of China(NSFC)(Grant Nos.41290255 and 41205046)
文摘This study examined the impact of the preceding boreal summer(June–August) North Atlantic Oscillation(NAO) on early autumn(September) rainfall over Central China(RCC). The results show that a significant positive correlation exists between the preceding summer NAO and the early autumn RCC on the interannual timescale. In order to understand the physical mechanism between them, the role of ocean was investigated. It was found that the strong summer NAO can induce a tripole sea surface temperature anomaly(SSTA) in the North Atlantic; this SSTA pattern can persist until early autumn. The diagnostic analysis showed that the tripole SSTA pattern excites a downstream Atlantic-Eurasian(AEA) teleconnection, which contributes to an increase in RCC. The circulation anomalies related to SSTA caused by the weak NAO are opposite, so the RCC is less than normal. The results imply that the preceding summer NAO may be regarded as a forecast factor for the early autumn RCC.
基金Under the auspices of Major Science and Technology Program for Water Pollution Control and Treatment(No.2012ZX07202-004-05)National Natural Science Foundation of China(No.41401352)Science and Enterprise Competitive Selection Project of Shenyang City,Shenyang Science and Technology Plan Project(No.F14-133-9-00)
文摘Heavy metal pollutants are a worldwide concern due to slow decomposition, biocondensation, and negative effects on human health. We investigated seasonal and spatial variations of the five heavy metals and evaluated their health risk in the Liaohe River, Northeast China. A total of 324 surface water samples collected from 2009 to 2010 were analyzed. Levels(high to low) of heavy metals in the Liaohe River were: zinc(Zn) > chromium(Cr) > copper(Cu) > cadmium(Cd) > mercury(Hg). Spatial and seasonal changes impacting concentrations of Cu and Zn were significant, but not significant for Cr, Cd and Hg. The highest concentrations of heavy metals were: Hg at Liuheqiao, Cu at Fudedian, Zn at Tongjiangkou, Cr at Mahushan, and Cd at Shenglitang. The highest concentrations of Hg and Cr were found in the wet period, Cu and Cd in the level period, and Zn in the dry period. The surface water of a tributary was an important accumulation site for heavy metals. Health risks from carcinogens and non-carcinogens increased from upstream to downstream in the mainstream of the Liaohe River. The total health risk for one person in the Liaohe River exceeded acceptable levels. The total health risk was the greatest during the wet period and least in the dry period. Among the five heavy metals in the Liaohe River, Cr posed the greatest single health risk.
文摘Wano, spoken by about 7,000 native speakers, is a Papuan language of Trans-New Guinea Phylum, Dani-Kwerba Stock, which is found in the interior of Papua of the regency of Puncak Jaya. The language is closely related to Dani, Walak, and Nggem. It is an SOV language typology that has a complex morphological system. Four spatial dimensions are morphosyntactically coded in elevative deixis, which are steepness/non-steepness distinction, proximity/distality distinction, adverbial/attributive expressions, and vertical/horizontal plane. This paper discusses the grammatical operation of a set of two-term system: ei "up" and ou "down" that serves as the basic forms for the elevational deixis in Wano.
基金Opening Fund of State Key Laboratory of Fire Science,University of Science and Technology of China(No.HZ2011-KF04)
文摘Water mist technology provides efficient firefighting performance while there is still room for improvement. So varieties of additives have been studied in recent years both at home and abroad. The self-made additives are used to compare the firefighting performance of diesel and heptane fire in open space. By adjusting the concentration of substance in the additives and conducting the experiment under the pressure of 0.3 MPa,0.5 MPa and 0.7 MPa,extinguish time and temperature are measured in the experiment. Through the experiments using different fuels,it can be found when the fuel is heptane that has a lower ignition point and a higher evaporation rate, the water mist additive can still significantly improve the firefighting performance. According to the data based on different concentrations of fluorinated surfactants,we find that fluorinated surfactants are the main substance to improve the performances by changing physical property of water mist. Optimal proportion of the additives for firefighting performance is found by experiment results.
基金NSFC (No. 40502008)Sichuan Province Application and Basic Research Project (No. 05JY029-008)
文摘In this study, X-ray diffraction (XRD), Fourier transform infrared spectrometer (FTIR) together with Scanning probe microscopy (SPM) were used to characterize the structure and morphology of the complexes, where the hydrobiotites (Xinfiang) were modified by single-chain surfactants octyltrimethylammonium bromide (OTMA) and octadecyltrimethylammonium bromide (ODTMA). XRD patterns showed that the structure of complexes was significantly influenced by the surfactant concentration and the alkyl chain length, because obvious changes took place in the basal spacing. Furthermore, according to the XRD results, several arrangements of surfactant molecules within the hydrobiotite interlayer space were deduced. The FTIR spectrum indicated that the surfactant contents in complexes dramatically increased with the alkyl chain length. The SPM micrographs demonstrated that the surfaces of complexes prepared at lower surfactant concentration were relatively flat compared with that prepared at higher concentration, while those with higher surfactant concentration had much steeper surface due to the alkyl chain length. It was concluded that structure and morphology of surfactant/hydrobiotite complexes depend not only on the surfactant concentration, but also strongly on the surfactant species.
基金Supported by the Scientific and Technological Project in Shaanxi Province (2005K09-G6-2)
文摘Objective: To study the effect and mechanism of aspirin alone or combined with cisplatin (DDP) on human cervical carcinoma HeLa cells. Methods: HeLa cells were treated by different concentrations of aspirin, DDP alone or both. The inhibitory effect on cell growth was analyzed by MTT and colony-forming assay. Cell apoptosis was measured with flow cytometry. The mRNA levels ofBcl-2, Bax and NF-κB(P65) were studied by RT-PCR. Results: MTT assay showed that aspirin inhibited HeLa cell proliferation in a time-and dose-dependant maoner. Aspirin decreased clone numbers in colony formation assay. Aspirin also induced apoptosis of HeLa cells in a dose- and time-dependent manner as detected by flow cytometry. The inhibition effects on proliferation, colony formation and apoptosis were significantly enhanced when cells were treated with both aspirin and DDP. RT-PCR demonstrated that aspirin decreased the transcription of Bcl-2 and NF-κB, and increased expression of Bax gene. Conclusion: Aspirin can induce apoptosis in HeLa cells. Combination of aspirin and DDP displays a synergistic effect. The possible mechanism might be that aspirin downregulates the mRNA levels of Bcl-2 and NF-κB gene and upregulates the expression of Bax.
基金sponsored by the Specialized Project of Earthquake Profession(201008007),CEA
文摘As the most import fault of the Late Pleistocene in the Lhasa area,the Nalinlaka fault is a left-lateral thrust fault,striking NWW,dipping SSW with a high dip angle,and extending over 33km.According to studies on the latest strata on the Nalinlaka fault zone,this fault zone has been obviously active since the Late Pleistocene and the movement left behind some geomorphologic phenomena on the earth's surface,especially at the sites of the gully west of Cijiaolin and around Xiecun village.For example,some rivers,ridges and terraces are displaced,forming beheaded gullies and fault escarps.The horizontal displacements since the Late Pleistocene at the above two places are 54m ~ 87m,20m ~ 67m,respectively.Based on studies on the 4 trenches along the fault using the progressive constraining method,we conclude that there might have been 5 paleoearthquake events along the Nalinlaka fault since 70ka B.P.,the ages of each paleoearthquake are 68.53,54.40,< 41.23,21.96,9.86ka B.P.,and the average recurrence interval is 14.67ka.Because of the limits of trenches and earthquake events exposed by each trench,no single trench completely revealed all 5 events.There may therefore be some errors in determining the upper and lower limits of some events in this article.
基金supported by the Key Project of National Natural Science Foundation‘‘Deep Heat Governance and Utilization’’(No.51134005)the Doctoral Fund of Ministry of Education(No.20120023120004)
文摘Regarding the lack of cold source for underground cooling systems from either mine inflow or return air, field experiments were taken in a high temperature deep coal mine with abundant cold source from surface water. Taking Sanhejian coal mine as an example, this paper introduced the technology scheme of heat disaster governance using surface water cold source. The paper presents the basics of this field experiment at the beginning, following by the design and site layout of the cooling system including the analysis and calculation of cold source. Numerical calculation method is also applied based on the operation parameters to simulate the influence to the surface river ecosystem. The results suggest that the temperature of surface water shall be lower than 34 ℃ after heat exchange, and when more cooling capacities are needed in the future, increasing the water flow is more favorable than increasing the cooling range of water, which is better for the ecological environment protection,
基金Supported by Major State Basic Research Program of China ("973" Program, No.2009CB118607)Inner Mongolia Science and Technology Foundation (No. 2009058)
文摘The reuse of wastewater for the irrigation of farmlands is gaining popularity, and the nutrient leaching associated with wastewater irrigation is becoming a matter of concern. The variability of nitrogen and phosphorus frac- tions in wastewater-irrigated soil was investigated in both horizontal and vertical directions in Kongjiaxiang, Tongliao, Inner Mongolia, China. The results showed that wastewater irrigation resulted in the concentrations of available N and P being 40.36% and 66.49% higher, respectively, than those with groundwater irrigation. Different forms of N and P exhibited significantly different distribution pattems. Higher concentrations of NO~ and total available N, as well as of those of Ca-P and total available P were observed near wastewater irrigation channels. Ca-P has a spatial distribu- tion pattern similar to that of available P. The concentrations of NO3 and NH4 were the highest in top soil and de- creased with depth. The complex interactions between nitrate leaching and nitrogen transformation processes (e.g., nitrification, denitrification, and mineralization) determined the vertical profile of NO3. The significant amount of NH~ loss inhibited its deep seepage. The Ca-bound compound contained more P than other inorganic fractions as a result of high Ca levels throughout the soil profile. The differences in the concentrations of Ca-bound P at different depths could be due to the upward flux or translocation of Ca from subsurface to surface soil and the sequestration of P.
基金supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry and Science and Techology Commission of Shanghai Municipality(Grant No.11DZ2260400)
文摘The freezing and melting process of a small water droplet on a superhydrophobic cold surface was investigated using the Laser Induced Fluorescence(LIF)technique.The superhydrophobic surface was prepared using a sol-gel method on a red copper test plate.From the obtained fluorescence images,the phase transition characteristics during the freezing and melting process of a water droplet were clearly observed.It was found that,at the beginning of the droplet freezing process,liquid water turned into ice at a very fast rate.Such phase transition process decreased gradually with time and the volume of frozen ice approached a constant value at the end of the icing process.In addition,the freezing time was found to reduce with the decrease of the test plate temperature.Besides,when the test plate temperature is relatively high,the effect of droplet volume on the freezing time is very significant.Over all,we provide some tentative insights into the microphysical process related to the icing and melting process of water droplets.
基金supported by the National Natural Science Foundation of China (No. 51579213)the National Key Research and Development Program of China (No. 2017YFC0403303)
文摘Water-repellent(WR) soil greatly influences infiltration behavior. This research determined the impacts of WR levels of silt loam soil layer during infiltration. Three column scenarios were utilized, including homogeneous wettable silt loam or sand, silt loam over sand(silt loam/sand), and sand over silt loam(sand/silt loam). A 5-cm thick silt loam soil layer was placed either at the soil surface or 5 cm below the soil surface. The silt loam soil used had been treated to produce different WR levels, wettable, slightly WR, strongly WR, and severely WR. As the WR level increased from wettable to severely WR, the cumulative infiltration decreased. Traditional wetting front-related equations did not adequately describe the infiltration rate and time relationships for layered WR soils. The Kostiakov equation provided a good fit for the first infiltration stage. Average infiltration rates for wettable, slightly WR, strongly WR, and severely WR during the 2 nd infiltration stage were 0.126, 0.021, 0.002, and 0.001 mm min^(-1) for the silt loam/sand scenario,respectively, and 0.112, 0.003, 0.002, and 0.000 5 mm min^(-1) for the sand/silt loam scenario, respectively. Pseudo-saturation phenomena occurred when visually examining the wetting fronts and from the apparent changes in water content(?θ_(AP)) at the slightly WR,strongly WR, and severely WR levels for the silt loam/sand scenario. Much larger ?θAPvalues indicated the possible existence of finger flow. Delayed water penetration into the surface soil for the strongly WR level in the silt loam/sand scenario suggested negative water heads with infiltration times longer than 10 min. The silt loam/sand soil layers produced sharp transition zones of water content. The WR level of the silt loam soil layer had greater effects on infiltration than the layer position in the column.
基金supported by the National High-Tech Research Program of China(Grant Nos.2009AA122001 and 2009AA122004)
文摘Land surface water(LSW) is one of the most important resources for human survival and development, and it is also a main component of global water recycling. A full understanding of the spatial distribution of land surface water and a continuous measuring of its dynamics can support to diagnose the global ecosystem and environment. Based on the Global Land 30-water 2000 and Global Land 30-water 2010 products, this research analyzed the spatial distribution pattern and temporal fluctuation of land surface water under scale-levels of global, latitude and longitude, continents, and climate zones. The Global Land 30-water products were corrected the temporal inconsistency of original remotely sensed data using MODIS time-series data, and then calculated the indices such as water area, water ration and coefficient of spatial variation for further analysis. Results show that total water area of land surface is about 3.68 million km2(2010), and occupies 2.73% of land area. The spatial distribution of land surface water is extremely uneven and is gathered mainly in mid- to high-latitude area of the Northern Hemisphere and tropic area. The comparison of water ratio between 2000 and 2010 indicates the overall fluctuation is small but spatially differentiated. The Global Land 30-water products and the statistics provided the fundamental information for analyzing the spatial distribution pattern and temporal fluctuation of land surface water and diagnosing the global ecosystem and environment.
文摘This paper describes the NiiMi process designed to treat landscape water. The main aim of the research was to investigate the feasibility of NiiMi for removing organic and nutriment materials from landscape water. During the batch-scale NiiMi operation, the removal rates of color ranged from 66.7%o-80% , of turbidity from 31.7%-89.3%o, of chemical oxygen demand (COD) from 7%-36.5%, of total phosphor (TP) from 43%-84.2%, of soluble phosphate from 42.9%-100%, of total nitrogen (TN) from 4.2%-46.7%, and of NH4^+-N from 39.3%-100% at the hydraulic loading of 0.2 m^3/(m^2·d). Results showed that the removal efficiencies of COD, TP, soluble phosphate and TN decreased with the decline in the temperature. The NiiMi process had a strong shock loading ability for the removal of the organics, turbidity, TP, soluble phosphate, TN and NH4^+-N. Three sodium chloride tracer studies were conducted, labeled as TS 1, TS2, and TS3, respectively. The mean hydraulic retention times (mean HRTs) were 31 h and 28 h for TSI and TS2, respectively, indicating the occurrence of a dead zone volume of 12% and 20% for TS 1 and TS2, respectively. TS 1 and TS2 displayed the occurrence of short-circuiting in the NiiMi system. The comparison results between TS1 and TS2 were further confirmed in the values obtained for some indicators, such as volumetric efficiency (e), short-circuiting (S), hydraulic efficiency (2) and number of continuously stirred tank reactors (N).