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固定化肌红蛋白在水-乙醇混合溶液中的类酶活性电化学
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作者 李业梅 刘慧宏 《分析试验室》 CAS CSCD 北大核心 2012年第4期64-68,共5页
用琼脂糖将肌红蛋白(Mb)固定到玻碳电极(GC)表面,制备了Mb-琼脂糖膜修饰电极。包埋在琼脂糖膜中的Mb在缓冲溶液和乙醇混合溶液中与电极直接传递电子,得到一对对称的Mb辅基血红素Fe(III)/Fe(II)电对的可逆氧化还原峰。其式电势随缓冲溶... 用琼脂糖将肌红蛋白(Mb)固定到玻碳电极(GC)表面,制备了Mb-琼脂糖膜修饰电极。包埋在琼脂糖膜中的Mb在缓冲溶液和乙醇混合溶液中与电极直接传递电子,得到一对对称的Mb辅基血红素Fe(III)/Fe(II)电对的可逆氧化还原峰。其式电势随缓冲溶液pH值增加而负移,且呈线性关系,这说明Mb的电子传递过程伴随有质子的转移。在缓冲溶液和乙醇混合溶液中,固定化肌红蛋白表现出类似细胞色素P450的催化活性,能快速催化还原氯乙烷(六氯乙烷、五氯乙烷、四氯乙烷)脱氯,Mb-琼脂糖膜修饰电极具有较好的稳定性和重现性,可用于这些物质的定量检测。 展开更多
关键词 肌红蛋白 琼脂糖 乙醇-混合溶液 电化学 电催化
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Distribution and transformation behaviors of heavy metals during liquefaction process of sewage sludge in ethanol-water mixed solvents 被引量:4
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作者 PAN Zi-qian HUANG Hua-jun +4 位作者 ZHOU Chun-fei LAI Fa-ying HE Xiao-wu XIONG Jiang-bo XIAO Xiao-feng 《Journal of Central South University》 SCIE EI CAS CSCD 2019年第10期2771-2784,共14页
Liquefaction of sewage sludge(SS)in ethanol-water cosolvents is a promising process for the preparation of bio-oil/biochar products.Effect of the combined use of ethanol and water on the distribution/transformation be... Liquefaction of sewage sludge(SS)in ethanol-water cosolvents is a promising process for the preparation of bio-oil/biochar products.Effect of the combined use of ethanol and water on the distribution/transformation behaviors of heavy metals(HMs)contained in raw SS is a key issue on the safety and cleanness of above liquefaction process,which is explored in this study.The results show that pure ethanol facilitates the migration of HMs into biochar products.Pure water yields lower percentages of HMs in mobile/bioavailable speciation.Compared with sole solvent treatment,ethanol-water cosolvent causes a random/average effect on the distribution/transformation behaviors of HMs.After liquefaction of SS in pure water,the contamination degree of HMs is mitigated from high level(25.8(contamination factor))in raw SS to considerable grade(13.4)in biochar and the ecological risk is mitigated from moderate risk(164.5(risk index))to low risk(78.8).Liquefaction of SS in pure ethanol makes no difference to the pollution characteristics of HMs.The combined use of ethanol and water presents similar immobilization effects on HMs to pure water treatment.The contamination factor and risk index of HMs in biochars obtained in ethanol-water cosolvent treatment are 13.1-14.6(considerable grade)and 79.3-101.0(low risk),respectively.In order to further control the pollution of HMs,it is preferentially suggested to improve the liquefaction process of SS in ethanol-water mixed solvents by introducing conventional lignocellulosic/algal biomass,also known as co-liquefaction treatment. 展开更多
关键词 sewage sludge LIQUEFACTION ethanol-water cosolvent heavy metals contamination degree ecological risk
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Heat Transfer Properties and Chaotic Analysis of Parallel Type Pulsating Heat Pipe 被引量:2
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作者 史维秀 李惟毅 +1 位作者 潘利生 谈西峰 《Transactions of Tianjin University》 EI CAS 2011年第6期435-439,共5页
The refrigerant mixture of ethanol aqueous was applied to the parallel type pulsating heat pipe (PHP). The operation characteristics of the PHP were analyzed by means of experiment and nonlinear chaotic theory. Moreov... The refrigerant mixture of ethanol aqueous was applied to the parallel type pulsating heat pipe (PHP). The operation characteristics of the PHP were analyzed by means of experiment and nonlinear chaotic theory. Moreover, the relationship between the running state and attractor was described. The results indicate that starting power, stable running power and dry burning transition power are about 64.08 W, 148.68 W and 234.0 W respectively. The cycle and amplitude of PHP initially decrease and then increase with the increasing power. However, the data are welldistributed in a certain range. The running state is in agreement with the attractors, and the changing process for attractors is as follows: the attractors first disperse in the whole phase space, then present mass status, and finally show band distribution. 展开更多
关键词 parallel type pulsating heat pipe ethanol aqueous ATTRACTOR chaotic analysis
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琼脂糖膜固载肌红蛋白催化还原过氧化物的研究 被引量:1
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作者 李业梅 刘慧宏 《分析试验室》 CAS CSCD 北大核心 2012年第5期80-83,共4页
用琼脂糖(agarose)将肌红蛋白(Mb)固定在玻碳电极(GCE)表面,制备了Mb-Agarose膜修饰电极。在水-乙醇混合溶液中,包埋在Agarose中的Mb与电极发生直接电子传递,并且能催化还原H2O2、过氧化丁酮、氢过氧化叔丁基、氢过氧化异丙基苯等过氧... 用琼脂糖(agarose)将肌红蛋白(Mb)固定在玻碳电极(GCE)表面,制备了Mb-Agarose膜修饰电极。在水-乙醇混合溶液中,包埋在Agarose中的Mb与电极发生直接电子传递,并且能催化还原H2O2、过氧化丁酮、氢过氧化叔丁基、氢过氧化异丙基苯等过氧化物和NO。Mb-Agarose膜修饰电极具有较好的稳定性和重现性,可用于上述过氧化物和亚硝酸盐的定量检测。 展开更多
关键词 肌红蛋白 电催化 过氧化物 亚硝酸盐 琼脂糖膜 水-乙醇混合溶液
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