在昆虫中,众所周知蜕皮类固醇在完成幼虫蜕皮和变态蜕皮中起关键作用,但蜕皮类型完全取决于蜕皮素释放时刻存在的保幼激素(JH)滴度(Williams,1961;Nijhout和Wheeler,1982)。实际上,在临界期对幼虫试验摘除脑和前胸腺,导致大多数昆虫幼...在昆虫中,众所周知蜕皮类固醇在完成幼虫蜕皮和变态蜕皮中起关键作用,但蜕皮类型完全取决于蜕皮素释放时刻存在的保幼激素(JH)滴度(Williams,1961;Nijhout和Wheeler,1982)。实际上,在临界期对幼虫试验摘除脑和前胸腺,导致大多数昆虫幼虫期的延长(Steel和Pavey,1985;Wigglesworth,1985)。用添食JH或其类似物(JHA)的方法,通过抑制变态也能延长许多昆虫的幼虫期(Sehnal,1983)。通过使末龄家蚕幼虫接受较大剂量的JHA,普遍诱导出永久幼虫(Akai和Kobayashi,1971;Akai等,1973)。在大多数情况中,在给予激素的龄期就显示出JH的影响,公认为是JH的现状作用the status guo action(Williams,1961)。因此。展开更多
Objective To identify new genes required for neurosecretory control of aging in C. elegans. Methods In view of the importance of nervous system in aging regulation, we performed the screen for genes involved in the ag...Objective To identify new genes required for neurosecretory control of aging in C. elegans. Methods In view of the importance of nervous system in aging regulation, we performed the screen for genes involved in the aging regulation from genetic loci encoding synaptic proteins by lifespan assay and accumulation of lipofuscin autofluorescence. We further investigated the dauer formation phenotypes of their corresponding mutants and whether they were possibly up-regulated by the insulin-like signaling pathway. Results The genetic loci of unc-10, syd-2, hlb-1, dlk-1, mkk-4, scd- 2, snb-1, ric-4, nrx-1, unc-13, sbt-1 and unc-64 might be involved in the aging control. In addition, functions of unc-10, syd-2, hlb-1, dlk-1, mkk-4, scd-2, snb-1, ric-4 and nrx-1 in regulating aging may be opposite to those of unc-13, sbt-1 and unc-64. The intestinal autofluorescence assay further indicated that the identified long-lived and short-lived mutants were actually due to the suppressed or accelerated aging. Among the identified genes, syd-2, hlb-1, mkk-4, scd-2, snb-1, ric-4 and unc-64 were also involved in the control of dauer formation. Moreover, daf-2 mutation positively regulated the expression of syd-2 and hlb-1, and negatively regulated the expression of mkk-4, nrx-1, ric-4, sbt-1, rpm-1, unc-10, dlk- 1 and unc-13. The daf-16 mutation positively regulated the expression of syd-2 and hlb-1, and negatively regulated the expression of mkk-4, nrx-1, sbt-1, rpm-1, unc-10, dlk-1 and unc-13. Conclusion These data suggest the possibly important status of the synaptic transmission to the animal＇ s life-span control machinery, as well as the dauer formation control.展开更多
文摘在昆虫中,众所周知蜕皮类固醇在完成幼虫蜕皮和变态蜕皮中起关键作用,但蜕皮类型完全取决于蜕皮素释放时刻存在的保幼激素(JH)滴度(Williams,1961;Nijhout和Wheeler,1982)。实际上,在临界期对幼虫试验摘除脑和前胸腺,导致大多数昆虫幼虫期的延长(Steel和Pavey,1985;Wigglesworth,1985)。用添食JH或其类似物(JHA)的方法,通过抑制变态也能延长许多昆虫的幼虫期(Sehnal,1983)。通过使末龄家蚕幼虫接受较大剂量的JHA,普遍诱导出永久幼虫(Akai和Kobayashi,1971;Akai等,1973)。在大多数情况中,在给予激素的龄期就显示出JH的影响,公认为是JH的现状作用the status guo action(Williams,1961)。因此。
文摘Objective To identify new genes required for neurosecretory control of aging in C. elegans. Methods In view of the importance of nervous system in aging regulation, we performed the screen for genes involved in the aging regulation from genetic loci encoding synaptic proteins by lifespan assay and accumulation of lipofuscin autofluorescence. We further investigated the dauer formation phenotypes of their corresponding mutants and whether they were possibly up-regulated by the insulin-like signaling pathway. Results The genetic loci of unc-10, syd-2, hlb-1, dlk-1, mkk-4, scd- 2, snb-1, ric-4, nrx-1, unc-13, sbt-1 and unc-64 might be involved in the aging control. In addition, functions of unc-10, syd-2, hlb-1, dlk-1, mkk-4, scd-2, snb-1, ric-4 and nrx-1 in regulating aging may be opposite to those of unc-13, sbt-1 and unc-64. The intestinal autofluorescence assay further indicated that the identified long-lived and short-lived mutants were actually due to the suppressed or accelerated aging. Among the identified genes, syd-2, hlb-1, mkk-4, scd-2, snb-1, ric-4 and unc-64 were also involved in the control of dauer formation. Moreover, daf-2 mutation positively regulated the expression of syd-2 and hlb-1, and negatively regulated the expression of mkk-4, nrx-1, ric-4, sbt-1, rpm-1, unc-10, dlk- 1 and unc-13. The daf-16 mutation positively regulated the expression of syd-2 and hlb-1, and negatively regulated the expression of mkk-4, nrx-1, sbt-1, rpm-1, unc-10, dlk-1 and unc-13. Conclusion These data suggest the possibly important status of the synaptic transmission to the animal＇ s life-span control machinery, as well as the dauer formation control.
