[Objective] This study aimed to observe the embryonic development process of Leiocassis crassilabris. [Method] Wild L. crassilabris collected from the Nanjing section of the Yangtze River was used as parent fish for i...[Objective] This study aimed to observe the embryonic development process of Leiocassis crassilabris. [Method] Wild L. crassilabris collected from the Nanjing section of the Yangtze River was used as parent fish for intensive breeding and propagation by artificial insemination. The entire embryonic development process of L. crassilabris from insemination to hatching out of larvae fish was observed consecutively. [Result] Fertilized eggs of L. crassilabris are spherical, and the egg diameter is about 2.2-2.4 mm after water absorption; under conditions of water temperature ranging from 27 to 28 ℃, the embryonic development of L. crassilabris from insemination to hatching out of larvae fish lasted 2 496 min, with a total accumulated temperature of 1 123-1 165 h·℃. [Conclusion] This study is advantageous to better understand the characteristics of embryonic development of L. crassilabris and provides basic biological data for protection and utilization of fish resources and other related work.展开更多
2013年春季,广东佛山养殖的长吻鮠Leiocassis longirostris大范围死亡。从病鱼体内分离到一株病原菌GDFS-01,通过菌落形态观察、革兰氏染色镜检及生理生化鉴定,结合16S r DNA序列测定与系统发育分析,鉴定为鮰爱德华氏菌Edwardsiella ict...2013年春季,广东佛山养殖的长吻鮠Leiocassis longirostris大范围死亡。从病鱼体内分离到一株病原菌GDFS-01,通过菌落形态观察、革兰氏染色镜检及生理生化鉴定,结合16S r DNA序列测定与系统发育分析,鉴定为鮰爱德华氏菌Edwardsiella ictaluri。采取腹部注射和浸泡感染试验均证实该菌有致病性,病症与自然发病症状一致。药敏试验表明:分离菌株对恩诺沙星、氟苯尼考、阿莫西林、洛美沙星、多西环素、磺胺甲基异唑、土霉素等7种药物敏感,对青霉素、氨苄西林、氟哌酸、四环素、万古霉素、利福平、克林霉素等药物呈耐药。本研究结果为长吻鮠病害的有效防控提供参考。展开更多
基金Supported by Science and Technology Support Program of Science and Technology Department of Jiangsu Province(BE2009335)~~
文摘[Objective] This study aimed to observe the embryonic development process of Leiocassis crassilabris. [Method] Wild L. crassilabris collected from the Nanjing section of the Yangtze River was used as parent fish for intensive breeding and propagation by artificial insemination. The entire embryonic development process of L. crassilabris from insemination to hatching out of larvae fish was observed consecutively. [Result] Fertilized eggs of L. crassilabris are spherical, and the egg diameter is about 2.2-2.4 mm after water absorption; under conditions of water temperature ranging from 27 to 28 ℃, the embryonic development of L. crassilabris from insemination to hatching out of larvae fish lasted 2 496 min, with a total accumulated temperature of 1 123-1 165 h·℃. [Conclusion] This study is advantageous to better understand the characteristics of embryonic development of L. crassilabris and provides basic biological data for protection and utilization of fish resources and other related work.
文摘2013年春季,广东佛山养殖的长吻鮠Leiocassis longirostris大范围死亡。从病鱼体内分离到一株病原菌GDFS-01,通过菌落形态观察、革兰氏染色镜检及生理生化鉴定,结合16S r DNA序列测定与系统发育分析,鉴定为鮰爱德华氏菌Edwardsiella ictaluri。采取腹部注射和浸泡感染试验均证实该菌有致病性,病症与自然发病症状一致。药敏试验表明:分离菌株对恩诺沙星、氟苯尼考、阿莫西林、洛美沙星、多西环素、磺胺甲基异唑、土霉素等7种药物敏感,对青霉素、氨苄西林、氟哌酸、四环素、万古霉素、利福平、克林霉素等药物呈耐药。本研究结果为长吻鮠病害的有效防控提供参考。
