[Objective] The aim was to screen out simple methods of DNA extraction and effective ISSR primers suitable to all germplasm materials of Camellia chekiangoleosa.[Method] The modified CTAB method was used in the extrac...[Objective] The aim was to screen out simple methods of DNA extraction and effective ISSR primers suitable to all germplasm materials of Camellia chekiangoleosa.[Method] The modified CTAB method was used in the extraction of the genomic DNA,50 ISSR primers from other Camellia plants were used in the ISSR-PCR amplification for 20 germplasm materials from 10 populations in Fujian,Zhejiang,Jiangxi and Anhui Province.[Result] Pure DNA could be obtained rapidly by using the improved CTAB method,and the 20 selected effective primers had rich polymorphism,clear bands and good repeatability.337 DNA bands were obtained,of which 281 bands were polymorphic,accounting for 83.4% of the total amplified bands.And 16.85 bands could be amplified with a primer,averagely.[Conclusion] The selected 20 primers could be effectively applied to ISSR analysis of the germplasm resources of C.chekiangoleosa in Zhejiang.展开更多
[Objective] The aim was to conduct investigation and risk assessment of the alien invasive plants in riparian zone of Dongjiang River.[Method] The field survey was carried out by the combination methods of sampling pl...[Objective] The aim was to conduct investigation and risk assessment of the alien invasive plants in riparian zone of Dongjiang River.[Method] The field survey was carried out by the combination methods of sampling plots investigation and route survey,and the whole Dongjiang River was covered.52 sampling points were set in the main streams,and 87 sampling points in tributaries streams,624 quadrats were set in total;multi-index comprehensive evaluation method was adopted for the risk assessment with 6 first-level indexes and 25 second-level indexes.[Result] Through the investigation on the riparian zone of Dongjiang River for 2 years,51 species of alien invasive plants were found,belonging to 17 families and 38 genera.The risk level assessment results of these 51 species showed that 18 species,such as Ageratum conyzoides,belonged to high-level risk with over 60 points;26 species,such as Chenopodium ambrosioides,were between 30 and 60 points which belonged to middle-level risk;7 species,such as Paspalum dilatatum,with less than 30 points belonged to low-level risk.[Conclusion] Basic data on the protection,water environmental management and functional zoning of riparian zone of Dongjiang River were provided.展开更多
[Objective] The aim of this study was to characterize the national regis- tered varieties selected from cotton regional trials in Yangtze River Valley (YaRV) in recent years. [Method] Cotton cultivar classification ...[Objective] The aim of this study was to characterize the national regis- tered varieties selected from cotton regional trials in Yangtze River Valley (YaRV) in recent years. [Method] Cotton cultivar classification and comprehensive evaluation index were set up based on national cotton registration standard. GGE biplot method was adopted to analyze the correlation of major breeding target characters of 53 national registered cotton varieties in cotton regional trials in YaRV during 1981-2012. According to the shift of check cultivars in cotton regional trials in the past, the cotton regional trial practice since 1981 was divided into five periods. The dynamic of cultivar type's proportion and the evaluation index scores was analyzed across the five periods. [Result] There existed intricate interrelationship among cotton breeding target traits, which constrained it necessary to construct indices for com- prehensive evaluation of cotton varieties. The dynamic of cultivar types in the five periods indicated that type II varieties emerged since Simian 3 period and then its proportion decreased gradually; type Ⅲ varieties maintained a certain proportion in each period and kept on the rise overall; type Ⅳvarieties occupied the majority pro- portion of registered cultivars before 1993, but a minor proportion since Simian 3 period. On the other side, the change trend of the evaluation index demonstrated that the varieties registered before 2003 did not pass the qualified line at present. The peak scores appeared in the varieties registered during 2004-2008. The scores of the varieties registered after 2009 were only slightly over the qualified line. [Conclusion] More attention should be paid to the improvement and evaluation of micronaire, so as to guide the simultaneous development of high yielding and fiber quality in cotton breeding and registration procedure in YaRV.展开更多
The Changjiang River (Yangtze) is one of the fastest growth areas of container transportation in China. Rail, road and water transportation have competed against each other for container transportation in the Chang-ji...The Changjiang River (Yangtze) is one of the fastest growth areas of container transportation in China. Rail, road and water transportation have competed against each other for container transportation in the Chang-jiang River main line and its delta area. It is of significance to assess these different transportation modes scientifically in order to organize container transportation efficiently in this area and make decision for integral plan and construction of transportation system in this area. This paper outlines application of fuzzy comprehensive evaluation to appraise different modes of typical direction of containers. Twelve assessment indexes were decided. Membership functions were formulated. Evaluation results indicated that road transportation was optimal mode in the Changjiang River delta area, however water transportation was the primary way in the Changjiang River main line.展开更多
The contamination and environmental risk assessment of the toxic elements in sediments from the middle-downstream (Zhuzhou-Changsha section) of the Xiangjiang River in Hunan Province of China were studied. The results...The contamination and environmental risk assessment of the toxic elements in sediments from the middle-downstream (Zhuzhou-Changsha section) of the Xiangjiang River in Hunan Province of China were studied. The results show that As, Cd, Pb and Zn are major contaminants in sediments, and average concentrations of these elements significantly exceed both the Control Standards for Pollutants in Sludge of China (GB4284-84) for agricultural use in acidic soils and the effect range median (ERM) values. The average concentrations of As, Cd and Pb in the river water slightly exceed the limit of Surface Water Environment Quality Standard (GB3838-2002). The concentrations of As and Cr in depth profiles extensively change, but slight changes are observed in Pb and Zn. Cd and Zn in most sediment samples can easily enter the food-chain and bring possible ecotoxicological risk to organisms living in sediments according to the risk assessment code.展开更多
The paper analyzes the characteristics of rice production in Heilongjiang Reclamation Area(HRA)and the achievements made through innovative research and practice,evaluates the standardized mode of rice cultivation and...The paper analyzes the characteristics of rice production in Heilongjiang Reclamation Area(HRA)and the achievements made through innovative research and practice,evaluates the standardized mode of rice cultivation and technical standards for rice production,which play a key role in guaranteeing the growth of grain total output in Heilongjiang reclamation area and make great contributions to the national grain safety.展开更多
This paper analyzed the current situation of industrial clusters and industrial cluster standardization in and summarized relevant practice in five aspects including the experiences of government planning, standards c...This paper analyzed the current situation of industrial clusters and industrial cluster standardization in and summarized relevant practice in five aspects including the experiences of government planning, standards construction, implementation of techn standards strategy, clusters pilot construction and increasing the supply of industrial cluster in Jiangsu. The problems and challenges during Jiangsu Jiangsu, alliance of public industry standardization construction were put forword to promote the development of jiangsu industrial clusters.展开更多
This article adopts the method of case study, thorough analysis of Zhanjiang Normal University in teacher education training model innovation practice, explore its talent training innovation goals: to cultivate beaut...This article adopts the method of case study, thorough analysis of Zhanjiang Normal University in teacher education training model innovation practice, explore its talent training innovation goals: to cultivate beauty, intelligence and physique full scale development "responsible, strong ability, good innovation". Came to the conclusion that the university talents training mode of innovative practice is specific to subjects and to students.展开更多
The Changjiang fault zone,also known as the Mufushan-Jiaoshan fault,is a famous fault located at the southern bank of the Changjiang River,near the Nanjing downtown area.Based on multidisciplinary data from shallow ar...The Changjiang fault zone,also known as the Mufushan-Jiaoshan fault,is a famous fault located at the southern bank of the Changjiang River,near the Nanjing downtown area.Based on multidisciplinary data from shallow artificial seismic explorations in the target detecting area(Nanjing city and the nearby areas),trenching and drilling explorations,classification of Quaternary strata and chronology dating data,this paper provides the most up-to-date results regarding activities of the Changjiang fault zone,including the most recent active time,activity nature,related active parameters,and their relation to seismic activity.展开更多
Vegetable fields in peri-urban areas receive large amounts of extraneous heavy metals because of rapid urbanization and industrialization in China. The concentrations of Cu, Zn, and Pb in 30 soil samples and 32 vegeta...Vegetable fields in peri-urban areas receive large amounts of extraneous heavy metals because of rapid urbanization and industrialization in China. The concentrations of Cu, Zn, and Pb in 30 soil samples and 32 vegetable samples, collected from 30 different sites in southern Jiangsu Province of China, were measured and their transfer from soil to vegetable was determined. The results showed that the soil samples had wide ranges of pH (4.25-7.85) and electrical conductivity (EC) (0.24-3.42 dS m^-1). Among the soil samples, there were four soil samples containing higher Cu and two soil samples containing higher Zn concentrations than those specified in the Chinese Soil Environmental Quality Standard II. However, no vegetable sample was found to contain a high level of Cu or Zn. In contrast, one vegetable sample contained 0.243 mg Pb kg^-1 FW, which was above the Chinese Food Hygiene Standard, whereas the corresponding soil Pb concentration was lower than the Chinese Soil Environmental Quality Standard II. The transfer coefficients of Cu of all vegetable samples exceeded the suggested coefficient range, implying that extraneous Cu had high mobility and bioavallability to vegetables. There was no significant correlation between extractable soil heavy metal concentrations with four kinds of extractants and soil pH, EC, heavy metal concentrations in vegetables and soils, except that soil pH correlated well with the extractable soil Cu, Zn, and Pb concentrations with 1.0 mol L-1 NH4NO3. Moreover, diethylenetriamine pentaacetic acid (DTPA) extraction method was a more efficient method of extracting heavy metals from the soils independent of soil pH and EC than other three methods used.展开更多
Water shortage is a major issue in northern China. With the transfer of China's economic center from south to north, Circum-Bohai Economic Circle has gradually formed and developed as the third pole of China's econo...Water shortage is a major issue in northern China. With the transfer of China's economic center from south to north, Circum-Bohai Economic Circle has gradually formed and developed as the third pole of China's economy after Yangtze River Delta and Pearl River Delta, the problem of water has inevitably become more and more serious. South-to-North Water Diversion and Sea Water Desalination are helpful to solve this dilemma, but some difficulties appeared during the course of the two projects. By contrast, sea ice desalination can be considered as a third way to alleviate freshwater crisis in Bohai Rim. Firstly, the amount of sea ice in Bohai Sea is as much as 1.27 billion m3 even in normal years. Then the salinity of sea ice is only 1/4 to 1/7 as that of sea water. And furthermore, desalinized water of sea ice can meet national water quality standard for drinking. Purposely use the abundant sea ice resources as water source for industry, agriculture and living is very likely to open a new path for the solution of freshwater shortage, which can promote rapid and healthy development of Circum-Bohai Economic Circle.展开更多
基金Supported by Project of Zhejiang Provincial Department of Scienceand Technology(2010C32043)~~
文摘[Objective] The aim was to screen out simple methods of DNA extraction and effective ISSR primers suitable to all germplasm materials of Camellia chekiangoleosa.[Method] The modified CTAB method was used in the extraction of the genomic DNA,50 ISSR primers from other Camellia plants were used in the ISSR-PCR amplification for 20 germplasm materials from 10 populations in Fujian,Zhejiang,Jiangxi and Anhui Province.[Result] Pure DNA could be obtained rapidly by using the improved CTAB method,and the 20 selected effective primers had rich polymorphism,clear bands and good repeatability.337 DNA bands were obtained,of which 281 bands were polymorphic,accounting for 83.4% of the total amplified bands.And 16.85 bands could be amplified with a primer,averagely.[Conclusion] The selected 20 primers could be effectively applied to ISSR analysis of the germplasm resources of C.chekiangoleosa in Zhejiang.
基金Supported by National Water Pollution Control and Management Technology Major Project of ChinaEcological Function Partition Research of Level Three and Level Four in Key Watershed(2012ZX07501002)the Fundamental Work Project of Ministry ofScience and Technology (2006FY111000)~~
文摘[Objective] The aim was to conduct investigation and risk assessment of the alien invasive plants in riparian zone of Dongjiang River.[Method] The field survey was carried out by the combination methods of sampling plots investigation and route survey,and the whole Dongjiang River was covered.52 sampling points were set in the main streams,and 87 sampling points in tributaries streams,624 quadrats were set in total;multi-index comprehensive evaluation method was adopted for the risk assessment with 6 first-level indexes and 25 second-level indexes.[Result] Through the investigation on the riparian zone of Dongjiang River for 2 years,51 species of alien invasive plants were found,belonging to 17 families and 38 genera.The risk level assessment results of these 51 species showed that 18 species,such as Ageratum conyzoides,belonged to high-level risk with over 60 points;26 species,such as Chenopodium ambrosioides,were between 30 and 60 points which belonged to middle-level risk;7 species,such as Paspalum dilatatum,with less than 30 points belonged to low-level risk.[Conclusion] Basic data on the protection,water environmental management and functional zoning of riparian zone of Dongjiang River were provided.
基金Supported by Key Special Project for Breeding and Cultivation of GMO Varieties(2012ZX08013015)Jiangsu Agriculture Science and Technology Innovation Fund(JASTIF,CX-12-5035)~~
文摘[Objective] The aim of this study was to characterize the national regis- tered varieties selected from cotton regional trials in Yangtze River Valley (YaRV) in recent years. [Method] Cotton cultivar classification and comprehensive evaluation index were set up based on national cotton registration standard. GGE biplot method was adopted to analyze the correlation of major breeding target characters of 53 national registered cotton varieties in cotton regional trials in YaRV during 1981-2012. According to the shift of check cultivars in cotton regional trials in the past, the cotton regional trial practice since 1981 was divided into five periods. The dynamic of cultivar type's proportion and the evaluation index scores was analyzed across the five periods. [Result] There existed intricate interrelationship among cotton breeding target traits, which constrained it necessary to construct indices for com- prehensive evaluation of cotton varieties. The dynamic of cultivar types in the five periods indicated that type II varieties emerged since Simian 3 period and then its proportion decreased gradually; type Ⅲ varieties maintained a certain proportion in each period and kept on the rise overall; type Ⅳvarieties occupied the majority pro- portion of registered cultivars before 1993, but a minor proportion since Simian 3 period. On the other side, the change trend of the evaluation index demonstrated that the varieties registered before 2003 did not pass the qualified line at present. The peak scores appeared in the varieties registered during 2004-2008. The scores of the varieties registered after 2009 were only slightly over the qualified line. [Conclusion] More attention should be paid to the improvement and evaluation of micronaire, so as to guide the simultaneous development of high yielding and fiber quality in cotton breeding and registration procedure in YaRV.
文摘The Changjiang River (Yangtze) is one of the fastest growth areas of container transportation in China. Rail, road and water transportation have competed against each other for container transportation in the Chang-jiang River main line and its delta area. It is of significance to assess these different transportation modes scientifically in order to organize container transportation efficiently in this area and make decision for integral plan and construction of transportation system in this area. This paper outlines application of fuzzy comprehensive evaluation to appraise different modes of typical direction of containers. Twelve assessment indexes were decided. Membership functions were formulated. Evaluation results indicated that road transportation was optimal mode in the Changjiang River delta area, however water transportation was the primary way in the Changjiang River main line.
基金Project (20507022) supported by the National Natural Science Foundation of ChinaProject (EREH050303) supported by the Foundation of Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health
文摘The contamination and environmental risk assessment of the toxic elements in sediments from the middle-downstream (Zhuzhou-Changsha section) of the Xiangjiang River in Hunan Province of China were studied. The results show that As, Cd, Pb and Zn are major contaminants in sediments, and average concentrations of these elements significantly exceed both the Control Standards for Pollutants in Sludge of China (GB4284-84) for agricultural use in acidic soils and the effect range median (ERM) values. The average concentrations of As, Cd and Pb in the river water slightly exceed the limit of Surface Water Environment Quality Standard (GB3838-2002). The concentrations of As and Cr in depth profiles extensively change, but slight changes are observed in Pb and Zn. Cd and Zn in most sediment samples can easily enter the food-chain and bring possible ecotoxicological risk to organisms living in sediments according to the risk assessment code.
文摘The paper analyzes the characteristics of rice production in Heilongjiang Reclamation Area(HRA)and the achievements made through innovative research and practice,evaluates the standardized mode of rice cultivation and technical standards for rice production,which play a key role in guaranteeing the growth of grain total output in Heilongjiang reclamation area and make great contributions to the national grain safety.
文摘This paper analyzed the current situation of industrial clusters and industrial cluster standardization in and summarized relevant practice in five aspects including the experiences of government planning, standards construction, implementation of techn standards strategy, clusters pilot construction and increasing the supply of industrial cluster in Jiangsu. The problems and challenges during Jiangsu Jiangsu, alliance of public industry standardization construction were put forword to promote the development of jiangsu industrial clusters.
文摘This article adopts the method of case study, thorough analysis of Zhanjiang Normal University in teacher education training model innovation practice, explore its talent training innovation goals: to cultivate beauty, intelligence and physique full scale development "responsible, strong ability, good innovation". Came to the conclusion that the university talents training mode of innovative practice is specific to subjects and to students.
基金sponsored by the Key Construction Programof the National Tenth"Five-year Plan"the Sub-project forthe Earthquake Active Fault Detecting Technology System(1-4-10)the Active Fault Detecting and Earthquake Risk Evaluation of Nanjing City
文摘The Changjiang fault zone,also known as the Mufushan-Jiaoshan fault,is a famous fault located at the southern bank of the Changjiang River,near the Nanjing downtown area.Based on multidisciplinary data from shallow artificial seismic explorations in the target detecting area(Nanjing city and the nearby areas),trenching and drilling explorations,classification of Quaternary strata and chronology dating data,this paper provides the most up-to-date results regarding activities of the Changjiang fault zone,including the most recent active time,activity nature,related active parameters,and their relation to seismic activity.
基金Project supported by the Knowledge Innovative Program of the Chinese Academy of Sciences (No.KZCX3-SW-435)the National Natural Science Foundation of China (Nos.40671095 and 30700480).
文摘Vegetable fields in peri-urban areas receive large amounts of extraneous heavy metals because of rapid urbanization and industrialization in China. The concentrations of Cu, Zn, and Pb in 30 soil samples and 32 vegetable samples, collected from 30 different sites in southern Jiangsu Province of China, were measured and their transfer from soil to vegetable was determined. The results showed that the soil samples had wide ranges of pH (4.25-7.85) and electrical conductivity (EC) (0.24-3.42 dS m^-1). Among the soil samples, there were four soil samples containing higher Cu and two soil samples containing higher Zn concentrations than those specified in the Chinese Soil Environmental Quality Standard II. However, no vegetable sample was found to contain a high level of Cu or Zn. In contrast, one vegetable sample contained 0.243 mg Pb kg^-1 FW, which was above the Chinese Food Hygiene Standard, whereas the corresponding soil Pb concentration was lower than the Chinese Soil Environmental Quality Standard II. The transfer coefficients of Cu of all vegetable samples exceeded the suggested coefficient range, implying that extraneous Cu had high mobility and bioavallability to vegetables. There was no significant correlation between extractable soil heavy metal concentrations with four kinds of extractants and soil pH, EC, heavy metal concentrations in vegetables and soils, except that soil pH correlated well with the extractable soil Cu, Zn, and Pb concentrations with 1.0 mol L-1 NH4NO3. Moreover, diethylenetriamine pentaacetic acid (DTPA) extraction method was a more efficient method of extracting heavy metals from the soils independent of soil pH and EC than other three methods used.
文摘Water shortage is a major issue in northern China. With the transfer of China's economic center from south to north, Circum-Bohai Economic Circle has gradually formed and developed as the third pole of China's economy after Yangtze River Delta and Pearl River Delta, the problem of water has inevitably become more and more serious. South-to-North Water Diversion and Sea Water Desalination are helpful to solve this dilemma, but some difficulties appeared during the course of the two projects. By contrast, sea ice desalination can be considered as a third way to alleviate freshwater crisis in Bohai Rim. Firstly, the amount of sea ice in Bohai Sea is as much as 1.27 billion m3 even in normal years. Then the salinity of sea ice is only 1/4 to 1/7 as that of sea water. And furthermore, desalinized water of sea ice can meet national water quality standard for drinking. Purposely use the abundant sea ice resources as water source for industry, agriculture and living is very likely to open a new path for the solution of freshwater shortage, which can promote rapid and healthy development of Circum-Bohai Economic Circle.