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加压CO_2对污染乳中金黄色葡萄球菌杀菌效果的研究
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作者 付瑞东 刘美凤 +1 位作者 姚春艳 李晓东 《食品工业》 北大核心 2012年第5期63-65,共3页
通过加压二氧化碳对人工污染金黄色葡萄球菌的乳进行处理,对时间,温度,压力三个单因素试验对金黄色葡萄球菌的杀菌情况进行研究。结果显示单因素试验中,伴随着因素水平的增加,杀菌率随之提高。然后选取三个因素中最适宜的条件进行正交试... 通过加压二氧化碳对人工污染金黄色葡萄球菌的乳进行处理,对时间,温度,压力三个单因素试验对金黄色葡萄球菌的杀菌情况进行研究。结果显示单因素试验中,伴随着因素水平的增加,杀菌率随之提高。然后选取三个因素中最适宜的条件进行正交试验,并通过方差分析得出压力因素对金黄色葡萄球菌杀菌效果最显著、温度次之,时间不显著。在7.5 MPa,45℃,60 min时,杀菌效果最好达到98%以上。 展开更多
关键词 加压CO2 污染乳 金黄色葡萄球菌 杀菌效果
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实时RT-PCR检测存活于乳中的单核细胞增多性李斯特菌 被引量:15
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作者 闫冰 姜毓君 +3 位作者 曲妍妍 毕宇涵 相丽 赵凤 《食品科学》 EI CAS CSCD 北大核心 2008年第2期292-296,共5页
单增李斯特菌是一种可在低温下生长、能引起人畜共患病的食源性致病菌。为克服传统PCR检测的假阳性问题,有效的检测单增李斯特活菌,本研究提取单增李斯特菌的总RNA并进行反转录,以单增李斯特菌的必要毒力基因hlyA为靶基因,设计特异性引... 单增李斯特菌是一种可在低温下生长、能引起人畜共患病的食源性致病菌。为克服传统PCR检测的假阳性问题,有效的检测单增李斯特活菌,本研究提取单增李斯特菌的总RNA并进行反转录,以单增李斯特菌的必要毒力基因hlyA为靶基因,设计特异性引物和TaqMan探针进行实时PCR检测,研究了检测的特异性和灵敏度,考查了对人工污染牛乳进行检测的应用性。结果表明,所用引物和探针能较好的扩增目的基因,而对其他食源性致病菌无交叉反应。单增李斯特菌经1h增菌,可检出3×102CFU/ml。而经3h增菌,活菌检测限可达30CFU/ml。人工污染牛乳样品经6h增菌,检测限为17CFU/ml。此方法可应用于乳中单增李斯特菌的快速检测和污染状况调查。 展开更多
关键词 实时RT-PCR 单增李斯特菌 hlyA 人工污染乳 活菌
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外墙涂料及其所用乳液的开发研究现状 被引量:7
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作者 易昌凤 《胶体与聚合物》 2001年第3期29-31,共3页
综述了国内外外墙涂料的现状及发展趋势 ,介绍了外墙涂料一些品种和性能 ,特别强调了超耐候抗污染型外墙乳液涂料的开发与应用 ;同时介绍了超耐候外墙乳液涂料用乳液的研究。
关键词 建筑涂料 聚合物 超耐候抗污染乳液涂料 外墙涂料 品种 性能
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家兔球虫病的综合防制
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作者 陈智敏 《福建农业》 2001年第2期18-19,共2页
免球虫病是家兔最常见、危害最严重的一种寄生虫病。常发生于高温高湿的梅雨季节,各品种、年龄的兔均可感染,尤其从断乳至3月龄的幼兔感染率最高,死亡率可达80%以上,成兔感染后不表现症状,但会带虫排卯囊,污染乳头,传染给哺乳仔兔。采... 免球虫病是家兔最常见、危害最严重的一种寄生虫病。常发生于高温高湿的梅雨季节,各品种、年龄的兔均可感染,尤其从断乳至3月龄的幼兔感染率最高,死亡率可达80%以上,成兔感染后不表现症状,但会带虫排卯囊,污染乳头,传染给哺乳仔兔。采集病兔粪便和肝、肠病变部位刮取屑物,用直接涂片法或饱和盐水浮集法镜检发现大量卵囊即可确诊。 展开更多
关键词 球虫病 家兔 综合防制 病兔 慢性型 急性型 饱和盐水浮集法 污染乳 直接涂片法 成兔
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Fouling-resistant Composite Membranes for Separation of Oil-in-water Microemulsions 被引量:8
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作者 王枢 褚良银 陈文梅 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2006年第1期37-45,共9页
Fouling-resistant ceramic-supported polymer composite membranes were developed for removal of oil-in-water (O/W) mieroemulsions. The composite membranes were featured with an asymmetric three-layer structure, i.e., ... Fouling-resistant ceramic-supported polymer composite membranes were developed for removal of oil-in-water (O/W) mieroemulsions. The composite membranes were featured with an asymmetric three-layer structure, i.e., a porous ceramic membrane substrate, a polyvinylidene fluoride (PVDF) ultrafiltration sub-layer, and a polyamide/polyvinyl alcohol (PVA) composite thin top-layer. The PVDF polymer was east onto the tubular porous ceramic membranes with an immersion precipitation method, and the polyamide/PVA composite thin top-layer was fabricated with an inteffaeial polymerization method. The effects of the sub-layer composition and the recipe in the inteffaeial polymerization for fabricating the top-layer on the structure and performance of composite membranes were systematically investigated. The prepared composite membranes showed a good performance for treating the O/W microemulsions with a mean diameter of about 2.41μm. At the operating pressure of 0.4MPa, the hydraulic permeability remained steadily about 190L·m^-2·h^-1, the oil concentration in the permeate was less than 1.6mg·L^-1, and the oil rejection coefficient was always higher than 98.5% throughout the operation from the beginning. 展开更多
关键词 composite membranes fouling-resistant oil/water separation MICROEMULSION interracial polymerization
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Nisin Use to Control L. innocua in Experimentally Contaminated Gombasek Sausage
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作者 A. Laukova P. Turek J. Nagy 《Journal of Agricultural Science and Technology(A)》 2011年第5期746-750,共5页
Meat is a rich nutrient matrix that provides a suitable environment for proliferation of meat spoilage microbiota and common food-bom pathogens, therefore adequate preservation has to be applied in order to preserve i... Meat is a rich nutrient matrix that provides a suitable environment for proliferation of meat spoilage microbiota and common food-bom pathogens, therefore adequate preservation has to be applied in order to preserve its safety and qualib:. Lantibiotic nisin is not conunonly used in the meat industry, in spite of its beneficial effect in e.g. dairy industry. Theretbre, here, the effect of nisin was searched in traditional fermented sausage Gombasek experimentally contaminated with Listeria innocua Lil strata Ihe antimicrobial effect of nisin was demonstrated by the Lil strain decrease at day 2 (after contamination) with the difference 1.79 logarithmic (log) cycles comparing with the control sausage (CLi). At week l, the difference 4.14 log cycles was noted in the experimental sausages comparing with CLi sausages with prolonging inhibitory effect up to week 2; although the difference was diminished (1.82 log cycles); but higher than at day 2, The pH values as well as lactic acid values were not influenced by the nisin treatment (pH value in the reference sausage was 5.0, in CLi 5.7 and in the experimental sausage E, 5.1; amount of lactic acid in t: was 0.099 mmol L^-1: and in CLi 0.107 mmol L^-1). 展开更多
关键词 NISIN SAUSAGE L. innocua CONTROL
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Treatment of Benzene,Toluene and Xylene Contaminated Air in a Bioactive Foam Emulsion Reactor 被引量:4
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作者 Farshid Ghorbani Shahna Faride Golbabaei +3 位作者 Javad Hamedi Hossein Mahjub Hossein Reza Darabi Seyed Jamaladdin Shahtaheri 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2010年第1期113-121,共9页
A novel bioactive foam emulsion bioreactor for benzene,toluene and xylene(BTX)contaminated air streams treatment has been developed.The gas-liquid interfacial area by biocompatible foam and driving force for mass tran... A novel bioactive foam emulsion bioreactor for benzene,toluene and xylene(BTX)contaminated air streams treatment has been developed.The gas-liquid interfacial area by biocompatible foam and driving force for mass transfer by a water immiscible organic phase were increased in this reactor.The effect of several parameters such as gas residence time,oxygen content,and organic phase concentration on bioreactor performance was studied. Experimental results showed an average elimination capacity(EC)of 220 g·m3·h -1with removal efficiency(RE) of 89.59%for BTX inlet concentration of 1 g·m3at 15 s gas residence time in the bioreactor.The statistical developed model predicted that the maximum elimination capacity of the reactor for BTX could be reached to 423.45 g·m3·h -1.Continues operation of the bioreactor with high EC and RE was demonstrated by optimizing the operational parameters of the bioreactor.Overall the results suggest that the bioreactor developed can be very effective systems to treat BTX vapors. 展开更多
关键词 air pollution control bioactive foamed reactor biodegradation BTX
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