文摘目的分析和评价罗氏Cobas 6000全自动电化学发光检测系统的携带污染率。方法以罗氏Cobas 6000测定乙肝表面抗原(hepatitis B surface antigen,HBsAg)为例,设定携带污染的检测方案,并分析检测结果。结果 3个浓度阳性血清(Ha、Hb、Hc)、阴性血清(L)测试结果均值(8785,2878,275,0.449)、标准偏差和变异系数(coefficient of variation,CV)分别为Ha(136,1.55%),Hb(66,2.28%),Hc(9,3.32%),L(0.025,5.51%)。4个通道3个浓度HBsAg的携带污染率分别为:1模块-1通道(0.000708,0.001367,0.004247),1模块-2通道(0.000035,-0.00011,0.008525),2模块-1通道(-0.00053,0.001398,-0.00281),2模块-2通道(0.000045,0.003092,0.003786)。结论罗氏Cobas 6000全自动电化学发光免疫分析仪携带污染未检出,能确保临床检测的准确性。