The study was conducted to study the rational setting position of sand moisture sensor in real-time control irrigation system for greenhouse tomato. Six treatments were designed according to horizontal distance and ve...The study was conducted to study the rational setting position of sand moisture sensor in real-time control irrigation system for greenhouse tomato. Six treatments were designed according to horizontal distance and vertical depth from sensor to dripper, different upper and lower water content limits were set for differ-ent growth stages, and indexes including tomato growth, root, photosynthetic param-eter, quality, WUE and yield were systematical y analyzed. The result showed that it is suitable to bury soil moisture sensor in sand field with a vertical depth and a horizontal distance both of 10 cm from the dripper.展开更多
Objective: To construct a recombinant plasmid containing the outer membrane protein 2 (Omp2) gene of Chlamydia trachomatis and express Omp2 in E.coli. Methods: The omp2 gene of C. trachomatis serovar D was cloned into...Objective: To construct a recombinant plasmid containing the outer membrane protein 2 (Omp2) gene of Chlamydia trachomatis and express Omp2 in E.coli. Methods: The omp2 gene of C. trachomatis serovar D was cloned into pQE30 vector following PCR amplification from genomic DNA. E. coli M15 transformants were induced to express the fusion protein by IPTG and the product was identified by SDS-PAGE and Western blot. Results: Confirmed by enzyme cleavage analysis and DNA sequencing, a correct recombinant plasmid pQE30/omp2 was constructed. The fusion protein from the transformants was approximately 60 kDa in size in SDS-PAGE analysis, which could specially react with anti-6 X His mouse monoclonal IgG antibodies. Conclusion: We successfully expressed Omp2 in E. coli M15, providing an efficient and simple system for assaying the immunological properties of Omp2.展开更多
[Objective] This study aimed to solve the problems of low seed germina- tion rate and difficult bareroot seedling transplanting in artificial cultivation of Arto- carpus nanchuanensis, a native species in China. [Meth...[Objective] This study aimed to solve the problems of low seed germina- tion rate and difficult bareroot seedling transplanting in artificial cultivation of Arto- carpus nanchuanensis, a native species in China. [Method] Under different seed treatment and seedling style, the seed germination rate and seedling emergence rate of A. nanchuanensis were investigated. [Result] tn both sand bed and direct sowing yellow soil, the seed germination rate, seedling survival rate and seedling emergence rate in the treatment II were all higher than those in the treatments I and III. The average seed germination rate, seedling survival rate and seedling emergence rate in sand beds were 1.5%, 16.27% and 13.95%, respectively higher than those in direct sowing yellow soil. The average seedling emergence rate in sand beds was 2.46% higher than that in containers. Among the 8 kinds of light matrix formulas, the growth of seedlings in the formula VIII was best. In the three treatment groups, the seedling emergence rates, seedling heights and ground diame- ters in the formula VIII were all significantly different from those in the other formu- las. The seedling growth in the formula I and III ranked second, while the seedling emergence rate, seedling height and ground diameter in the formula VII were the poorest. [Conclusion] If conditions allow, the formulas I （meadow soil：vermiculite：yel- low soi1=5：3：2）, III （meadow soil：perlite：yellow soi1=5：3：2） and VIII （peat：forest top soi1=6：4） should be promoted in seedling with light matrixes.展开更多
AIM: To construct a recombinant attenuated Salmonella typhimurium DNA vaccine carrying Helicobacter pylori hpaA gene and to detect its immunogenicity. METHODS: Genomic DNA of the standard H pylori strain 17 874 was is...AIM: To construct a recombinant attenuated Salmonella typhimurium DNA vaccine carrying Helicobacter pylori hpaA gene and to detect its immunogenicity. METHODS: Genomic DNA of the standard H pylori strain 17 874 was isolated as the template, hpaA gene fragment was amplified by polymerase chain reaction (PCR) and cloned into pUCmT vector. DNA sequence of the amplified hpaA gene was assayed, then doned into the eukaryotic expression vector pIRES through enzyme digestion and ligation reactions. The recombinant plasmid was used to transform competent Escherichia coliDH5α, and the positive clones were screened by PCR and restriction enzyme digestion. Then, the recombinant pIRES-hpaA was used to transform LB5000 and the recombinant plasmid isolated from LB5000 was finally used to transform SL7207. After that, the recombinant strain was grown in vitro repeatedly. In order to identify the immunogenicity of the vaccine in vitro, the recombinant pIRES-hpaA was transfected to COS-7 cells using Lipofectamine^(TM)2000, the immunogenicity of expressed HpaA protein was detected with SDS-PAGE and Western blot. RESULTS: The 750-base pair hpaA gene fragment was amplified from the genomic DNA and was consistent with the sequence of H pylori hpaA by sequence analysis. It was confirmed by PCR and restriction enzyme digestion that H pylori hpaA gene was inserted into the eukaryotic expression vector pIRES and a stable recombinant live attenuated Salmonella typhimurium DNA vaccine carrying H pylori hpaA gene was successfully constructed and the specific strip of HpaA expressed by pIRES-hpaA was detected through Western blot. CONCLUSION: The recombinant attenuated Salmonella typhimurium DNA vaccine strain expressing HpaA protein with immunogenicity can be constructed and it may be helpful for further investigating the immune action of DNA vaccine in vivo.展开更多
This paper deals with the design, construction and performance evaluation procedure of a solar tunnel dryer in drying fish. A 12 meter long and 2 meter width half-circled tunnel was designed and constructed to dry abo...This paper deals with the design, construction and performance evaluation procedure of a solar tunnel dryer in drying fish. A 12 meter long and 2 meter width half-circled tunnel was designed and constructed to dry about 50-100 kg of freshly harvested fishes per batch. The half of the tunnel base was used as the flat plate air heating solar collector and the remaining half as a dryer. The drying air was forced from the collector region （north side） to the drying region （south side） of the half circled tunnel where the product is to be dried. The drying temperature could be easily raised by some 5-30 ℃ above the ambient temperature inside the tunnel at an air velocity of approximately 0.2 m/sec. The test was conducted with 51.5 kg freshly harvested sardines （hall-load） with initial moisture content of 66.5% （wet-basis） to analyze the performance of the dryer. The fishes were dried to a final average moisture content of 15.5% （wet-basis） within three days （30 hours）. It was possible to reach the moisture content level for safe storage within less than three days （30 hrs） with solar tunnel dryer and 7 days in open air natural sun drying. The improvement in the quality of fishes in terms of color, brightness, flavor, and taste and food value was distinctly recognized.展开更多
The seasonal transport of the Saharan dust to the West African region, near the Gulf of Guinea, during northem winter has been studied over 12 years. Using an optical particle counter, the dust aerosols in the diamete...The seasonal transport of the Saharan dust to the West African region, near the Gulf of Guinea, during northem winter has been studied over 12 years. Using an optical particle counter, the dust aerosols in the diameter range 0.5-25 μm have been sampled at Kumasi （6040＇ N, l°34＇ W） in Ghana during the winter months of January-February from 1997 to 2009. The settling atmospheric dust particles observed during the peak Harmattan and the background Harmattan periods are analysed for the mean particle size, number and mass concentrations as well as the particle size-frequency. It is shown that the average daily particle diameter, number and mass concentrations obtained in the peak Harmattan periods are 1.57 ± 0.54 μm, 50 ± 25 particles/cm3 and 1,130 ± 994 μg/m3, respectively, while for the background Harmattan these values are correspondingly, 1.31 ±0.31 μm, 32 ±12 particles/cm3 and 576 ±429 μg/m3, respectively. These experimental results will be useful for the design of ambient air-filters and for understanding the West African climate change.展开更多
This paper focuses on the functions of controlling in Estonia and Germany, The analysis based on the study carried out in Estonian enterprises in 2008. The study was conducted by the Estonian work group of the Interna...This paper focuses on the functions of controlling in Estonia and Germany, The analysis based on the study carried out in Estonian enterprises in 2008. The study was conducted by the Estonian work group of the International Controller Association ICV （internationaler controller verein）. The methodology and controlling questionnaire were the same, which were used by the German work group of ICV in 2006. The mentioned questionnaire was also worked out in Germany by Otto Beisheim School of Management. The authors compared the results of both studies Cultural differences and their impact on the tasks of controllers were also scrutinized. The results of the research indicate that the tasks of Estonian controllers are strictly fixed with procedure documents, which leaves very lithe room lbr independence and initiative. Usually, they are not involved in the strategic planning and goal setting. The final conclusion is that the sustainability function of controlling is much more on the background compared to other functions of controlling, but in the future the role of this function is expected to increase展开更多
基金Supported by National Science-technology Support Plan Project(2014BAD05B02)Ningxia Science-technology Support Plan Project(201522N03)~~
文摘The study was conducted to study the rational setting position of sand moisture sensor in real-time control irrigation system for greenhouse tomato. Six treatments were designed according to horizontal distance and vertical depth from sensor to dripper, different upper and lower water content limits were set for differ-ent growth stages, and indexes including tomato growth, root, photosynthetic param-eter, quality, WUE and yield were systematical y analyzed. The result showed that it is suitable to bury soil moisture sensor in sand field with a vertical depth and a horizontal distance both of 10 cm from the dripper.
基金This work was supported in part by grants from the Department of Science and Technology of Hunan Province (No. 01SSY2008-6) the Department of Health of Hunan Province (No. B2003-078).
文摘Objective: To construct a recombinant plasmid containing the outer membrane protein 2 (Omp2) gene of Chlamydia trachomatis and express Omp2 in E.coli. Methods: The omp2 gene of C. trachomatis serovar D was cloned into pQE30 vector following PCR amplification from genomic DNA. E. coli M15 transformants were induced to express the fusion protein by IPTG and the product was identified by SDS-PAGE and Western blot. Results: Confirmed by enzyme cleavage analysis and DNA sequencing, a correct recombinant plasmid pQE30/omp2 was constructed. The fusion protein from the transformants was approximately 60 kDa in size in SDS-PAGE analysis, which could specially react with anti-6 X His mouse monoclonal IgG antibodies. Conclusion: We successfully expressed Omp2 in E. coli M15, providing an efficient and simple system for assaying the immunological properties of Omp2.
基金Supported by Wildlife Conservation and Nature Reserve Construction Projects of Chongqing City in 2013~~
文摘[Objective] This study aimed to solve the problems of low seed germina- tion rate and difficult bareroot seedling transplanting in artificial cultivation of Arto- carpus nanchuanensis, a native species in China. [Method] Under different seed treatment and seedling style, the seed germination rate and seedling emergence rate of A. nanchuanensis were investigated. [Result] tn both sand bed and direct sowing yellow soil, the seed germination rate, seedling survival rate and seedling emergence rate in the treatment II were all higher than those in the treatments I and III. The average seed germination rate, seedling survival rate and seedling emergence rate in sand beds were 1.5%, 16.27% and 13.95%, respectively higher than those in direct sowing yellow soil. The average seedling emergence rate in sand beds was 2.46% higher than that in containers. Among the 8 kinds of light matrix formulas, the growth of seedlings in the formula VIII was best. In the three treatment groups, the seedling emergence rates, seedling heights and ground diame- ters in the formula VIII were all significantly different from those in the other formu- las. The seedling growth in the formula I and III ranked second, while the seedling emergence rate, seedling height and ground diameter in the formula VII were the poorest. [Conclusion] If conditions allow, the formulas I （meadow soil：vermiculite：yel- low soi1=5：3：2）, III （meadow soil：perlite：yellow soi1=5：3：2） and VIII （peat：forest top soi1=6：4） should be promoted in seedling with light matrixes.
基金Supported by the National Natural Science Foundation of China,No. 30170427
文摘AIM: To construct a recombinant attenuated Salmonella typhimurium DNA vaccine carrying Helicobacter pylori hpaA gene and to detect its immunogenicity. METHODS: Genomic DNA of the standard H pylori strain 17 874 was isolated as the template, hpaA gene fragment was amplified by polymerase chain reaction (PCR) and cloned into pUCmT vector. DNA sequence of the amplified hpaA gene was assayed, then doned into the eukaryotic expression vector pIRES through enzyme digestion and ligation reactions. The recombinant plasmid was used to transform competent Escherichia coliDH5α, and the positive clones were screened by PCR and restriction enzyme digestion. Then, the recombinant pIRES-hpaA was used to transform LB5000 and the recombinant plasmid isolated from LB5000 was finally used to transform SL7207. After that, the recombinant strain was grown in vitro repeatedly. In order to identify the immunogenicity of the vaccine in vitro, the recombinant pIRES-hpaA was transfected to COS-7 cells using Lipofectamine^(TM)2000, the immunogenicity of expressed HpaA protein was detected with SDS-PAGE and Western blot. RESULTS: The 750-base pair hpaA gene fragment was amplified from the genomic DNA and was consistent with the sequence of H pylori hpaA by sequence analysis. It was confirmed by PCR and restriction enzyme digestion that H pylori hpaA gene was inserted into the eukaryotic expression vector pIRES and a stable recombinant live attenuated Salmonella typhimurium DNA vaccine carrying H pylori hpaA gene was successfully constructed and the specific strip of HpaA expressed by pIRES-hpaA was detected through Western blot. CONCLUSION: The recombinant attenuated Salmonella typhimurium DNA vaccine strain expressing HpaA protein with immunogenicity can be constructed and it may be helpful for further investigating the immune action of DNA vaccine in vivo.
文摘This paper deals with the design, construction and performance evaluation procedure of a solar tunnel dryer in drying fish. A 12 meter long and 2 meter width half-circled tunnel was designed and constructed to dry about 50-100 kg of freshly harvested fishes per batch. The half of the tunnel base was used as the flat plate air heating solar collector and the remaining half as a dryer. The drying air was forced from the collector region （north side） to the drying region （south side） of the half circled tunnel where the product is to be dried. The drying temperature could be easily raised by some 5-30 ℃ above the ambient temperature inside the tunnel at an air velocity of approximately 0.2 m/sec. The test was conducted with 51.5 kg freshly harvested sardines （hall-load） with initial moisture content of 66.5% （wet-basis） to analyze the performance of the dryer. The fishes were dried to a final average moisture content of 15.5% （wet-basis） within three days （30 hours）. It was possible to reach the moisture content level for safe storage within less than three days （30 hrs） with solar tunnel dryer and 7 days in open air natural sun drying. The improvement in the quality of fishes in terms of color, brightness, flavor, and taste and food value was distinctly recognized.
文摘The seasonal transport of the Saharan dust to the West African region, near the Gulf of Guinea, during northem winter has been studied over 12 years. Using an optical particle counter, the dust aerosols in the diameter range 0.5-25 μm have been sampled at Kumasi （6040＇ N, l°34＇ W） in Ghana during the winter months of January-February from 1997 to 2009. The settling atmospheric dust particles observed during the peak Harmattan and the background Harmattan periods are analysed for the mean particle size, number and mass concentrations as well as the particle size-frequency. It is shown that the average daily particle diameter, number and mass concentrations obtained in the peak Harmattan periods are 1.57 ± 0.54 μm, 50 ± 25 particles/cm3 and 1,130 ± 994 μg/m3, respectively, while for the background Harmattan these values are correspondingly, 1.31 ±0.31 μm, 32 ±12 particles/cm3 and 576 ±429 μg/m3, respectively. These experimental results will be useful for the design of ambient air-filters and for understanding the West African climate change.
文摘This paper focuses on the functions of controlling in Estonia and Germany, The analysis based on the study carried out in Estonian enterprises in 2008. The study was conducted by the Estonian work group of the International Controller Association ICV （internationaler controller verein）. The methodology and controlling questionnaire were the same, which were used by the German work group of ICV in 2006. The mentioned questionnaire was also worked out in Germany by Otto Beisheim School of Management. The authors compared the results of both studies Cultural differences and their impact on the tasks of controllers were also scrutinized. The results of the research indicate that the tasks of Estonian controllers are strictly fixed with procedure documents, which leaves very lithe room lbr independence and initiative. Usually, they are not involved in the strategic planning and goal setting. The final conclusion is that the sustainability function of controlling is much more on the background compared to other functions of controlling, but in the future the role of this function is expected to increase
