Chlamydia Trachomatis and Ureaplasma Urealyticum Transmission Between Regular Sexual Partners

Objective: To investigate the transmission ratio andepidemiology of Chlamydia trachomatis (Ct) andUreaplasma urealyticum (Uu) between regular sexualpartners. Methods: We collected secretion specimens of geni-tourinary tract from sexual partners who attended ourclinic during the period from May 1, 1999 to May 31,2000 and then detected Ct and Uu in the specimens. Results: The inconsistent rate of Ct and Uu infectedbetween sexual partners was 59.22% and 38.92%respectively. There were no correlations between pa-tients' age and the inconsistent rate of Ct and Uu in-fected (P>0.05). The most common inconsistency be-tween regular partners was seen in man with Ct infec-tion while in female with Uu infection; and in manwith concurrent infection of Ct while in female onlywith Uu; or in man with Ct infection while in female noCt and Uu were detected. Conclusion: The sexual transmitting ability of Ctand Uu is not as active as we have expected.

性病病原体多重聚合酶链反应检测方法的建立和验证

目的建立同时检测淋球菌、沙眼衣原体、解脲支原体和单纯疱疹病毒-2致病微生物的多重聚合酶链反应(PCR)方法,用于四种性病的诊断和治疗监测。方法根据淋球菌外膜孔蛋白基因、沙眼衣原体基因、解脲支原体尿素酶基因和单纯疱疹病毒-2的DNA聚合酶基因序列,各设计一对特异性引物,优化反应体系和条件进行多重PCR反应。结果 4对引物分别扩增出327、167、426、391bp的目的条带,并且特异性强,与其他非目的病原体基因不发生反应。结论本研究建立的多重PCR方法为相关病原体的快速检测提供方法。