Aim To study the chemical constituents of the root and rhizome of Rheum tanguticum Maxim. ex Balf. Methods Chemical constituents were isolated and purified by many chromatographic methods, and their structures were el...Aim To study the chemical constituents of the root and rhizome of Rheum tanguticum Maxim. ex Balf. Methods Chemical constituents were isolated and purified by many chromatographic methods, and their structures were elucidated by MS, NMR, and others. Results Twenty compounds were isolated and their structures were identified as β-sitosterol, chrysophanol, aloe-emodin, physcion, rhein, emodin, etc. Conclusion Among these compounds, 4-(4′-hydroxyphenyl)-2-butanone, 4-(4′-hydroxyphenyl)-2-butanone-4′-O-β-D-(2″-...展开更多
目的通过建立白芍药材、饮片、标准汤剂HPLC特征图谱对白芍生产过程的共有峰进行定性分析,结合白芍药材-饮片-标准汤剂质量传递过程筛选白芍质量标志物(Q-Marker)。方法采集10批白芍鲜药材,制备为药材、饮片和标准汤剂,采用HPLC建立白...目的通过建立白芍药材、饮片、标准汤剂HPLC特征图谱对白芍生产过程的共有峰进行定性分析,结合白芍药材-饮片-标准汤剂质量传递过程筛选白芍质量标志物(Q-Marker)。方法采集10批白芍鲜药材,制备为药材、饮片和标准汤剂,采用HPLC建立白芍药材-饮片-标准汤剂特征图谱,结合相似度评价及正交偏最小二乘-判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)等分析初步确定的指标成分在药材-饮片-标准汤剂过程的含量及转移率,探究白芍生产过程中的质量传递规律,综合筛选白芍Q-Marker。结果白芍药材、饮片、标准汤剂特征图谱的共有峰有9个,组内相似度较高,OPLS-DA结果显示,9号共有峰不能稳定传递;白芍药材-饮片传递过程中,没食子酸、氧化芍药苷、儿茶素、芍药内酯苷、芍药苷、没食子酰芍药苷、1,2,3,4,6-O-五没食子酰葡萄糖、苯甲酰芍药苷8种成分转移率大于70%,可以传递稳定;饮片-标准汤剂传递过程中,芍药苷、芍药内酯苷、没食子酰芍药苷、氧化芍药苷、苯甲酰芍药苷等单萜及其苷类成分含量稳定且转移率大于60%,可以传递稳定;没食子酸转移率大于200%,未能充分体现白芍饮片中没食子酸的传递情况;儿茶素和1,2,3,4,6-O-五没食子酰葡萄糖2种成分转移率小于25%。结论结合白芍药材、饮片和标准汤剂中特征图谱、含量及转移率变化等,综合筛选确定氧化芍药苷、芍药内酯苷、芍药苷、没食子酰芍药苷、苯甲酰芍药苷5种成分作为白芍Q-Marker。展开更多
基金Natural Research Foundation of Qinghai Province( No. 2004-N-103-02)
文摘Aim To study the chemical constituents of the root and rhizome of Rheum tanguticum Maxim. ex Balf. Methods Chemical constituents were isolated and purified by many chromatographic methods, and their structures were elucidated by MS, NMR, and others. Results Twenty compounds were isolated and their structures were identified as β-sitosterol, chrysophanol, aloe-emodin, physcion, rhein, emodin, etc. Conclusion Among these compounds, 4-(4′-hydroxyphenyl)-2-butanone, 4-(4′-hydroxyphenyl)-2-butanone-4′-O-β-D-(2″-...
文摘目的通过建立白芍药材、饮片、标准汤剂HPLC特征图谱对白芍生产过程的共有峰进行定性分析,结合白芍药材-饮片-标准汤剂质量传递过程筛选白芍质量标志物(Q-Marker)。方法采集10批白芍鲜药材,制备为药材、饮片和标准汤剂,采用HPLC建立白芍药材-饮片-标准汤剂特征图谱,结合相似度评价及正交偏最小二乘-判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)等分析初步确定的指标成分在药材-饮片-标准汤剂过程的含量及转移率,探究白芍生产过程中的质量传递规律,综合筛选白芍Q-Marker。结果白芍药材、饮片、标准汤剂特征图谱的共有峰有9个,组内相似度较高,OPLS-DA结果显示,9号共有峰不能稳定传递;白芍药材-饮片传递过程中,没食子酸、氧化芍药苷、儿茶素、芍药内酯苷、芍药苷、没食子酰芍药苷、1,2,3,4,6-O-五没食子酰葡萄糖、苯甲酰芍药苷8种成分转移率大于70%,可以传递稳定;饮片-标准汤剂传递过程中,芍药苷、芍药内酯苷、没食子酰芍药苷、氧化芍药苷、苯甲酰芍药苷等单萜及其苷类成分含量稳定且转移率大于60%,可以传递稳定;没食子酸转移率大于200%,未能充分体现白芍饮片中没食子酸的传递情况;儿茶素和1,2,3,4,6-O-五没食子酰葡萄糖2种成分转移率小于25%。结论结合白芍药材、饮片和标准汤剂中特征图谱、含量及转移率变化等,综合筛选确定氧化芍药苷、芍药内酯苷、芍药苷、没食子酰芍药苷、苯甲酰芍药苷5种成分作为白芍Q-Marker。