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高沥水性钝顶螺旋藻新品系的选育及超微结构与RAPD分析 被引量:2
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作者 李晨晨 汪凡越 +2 位作者 汪志平 卢奇奇 陈子元 《微生物学报》 CAS CSCD 北大核心 2020年第2期237-245,共9页
[目的]选育高沥水性的螺旋藻新品系,显著降低藻粉生产中干燥的能耗。[方法]以用于工厂化培植的钝顶螺旋藻ZJU0115为出发品系,用组织匀浆-离心沉降法制得其原生质球,并先以0.6%EMS处理30 min再用2.4 kGy的60Coγ射线辐照,经含0.02%黄原胶... [目的]选育高沥水性的螺旋藻新品系,显著降低藻粉生产中干燥的能耗。[方法]以用于工厂化培植的钝顶螺旋藻ZJU0115为出发品系,用组织匀浆-离心沉降法制得其原生质球,并先以0.6%EMS处理30 min再用2.4 kGy的60Coγ射线辐照,经含0.02%黄原胶(xanthan gum)的Zarrouk’s培养液筛选、藻丝单体分离培养、藻泥持水率和胞外多糖(EPS)等检测及生产培植试验。[结果]获得了一株产量、蛋白质和多糖含量与ZJU0115相当,而藻泥持水率和EPS含量分别下降5.9%和29.7%的突变体,命名为ZJU0115(HD)。超微结构与随机扩增多态性DNA标记(random amplified polymorphic DNA,RAPD)分析结果显示,与其亲本ZJU0115相比,ZJU0115(HD)藻丝表面更光滑,可能为酸性多糖的乳白状粘附物也更少;ZJU0115(HD)细胞内的多磷酸盐颗粒显著变小且呈弥散状;基因组DNA在随机引物S90的扩增产物中显示出多态性差异。[结论]ZJU0115(HD)在工厂化培植中生产性状好、高沥水性能稳定,藻泥的干燥能耗降低了近50%,它的育成与应用,有助于推进当前螺旋藻产业迈向高效、节能、绿色、环保发展的新阶段。 展开更多
关键词 钝顶螺旋藻 沥水性 突变体 育种 超微结极 随机扩增多态DNA标记
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车身涂装SE分析过程要点 被引量:2
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作者 赵永欢 霍鹏 +1 位作者 刘哲 华冰 《现代涂料与涂装》 CAS 2016年第7期53-56,59,共5页
SE分析工作在整车开发过程中起着至关重要的作用,它主要是把缺陷和制造风险消除在设计阶段,本文重点从分析过程及分析要点方面进行了阐述,总结并分享实际工作过程中积累的经验。
关键词 SE 电泳屏蔽 排气 沥水性 涂胶
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Bond condition between surface layer and immediate layer in porous asphalt pavement 被引量:3
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作者 杨军 戴鹏 +3 位作者 于良溟 葛苏闽 刘清泉 钱国超 《Journal of Southeast University(English Edition)》 EI CAS 2007年第4期588-593,共6页
Through the shear tests on composite specimens using four different kinds of tack coat material (epoxy resin, SBS modified emulsified asphalt, SBS modified asphalt and H# bridge waterproof material), the bond condit... Through the shear tests on composite specimens using four different kinds of tack coat material (epoxy resin, SBS modified emulsified asphalt, SBS modified asphalt and H# bridge waterproof material), the bond condition between layers of porous asphalt pavement under traffic load, temperature variation and moisture situation is evaluated. The test results show that the bond strength decreases with the rise in temperature, and the relationship between shear strength and temperature can be expressed by a logarithm curve at a high reliability. Under the action of traffic load, the value of shear strength of the mixture right under the centre of the wheel track is smaller than that of other parts of the pavement. It is also found that some effects concerning moisture have comparative effects on the bonding of the two layers. Given all the results achieved during the study, it will be quite rewarding to make rational comparisons during selecting the sound type of tack coat. 展开更多
关键词 porous asphalt pavement waterproof and cohesive layer: shear strength
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The effect of cyclic hardening on fatigue properties of modified asphalt
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作者 袁燕 张肖宁 邹桂莲 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2007年第4期557-560,共4页
The phenomenon of cyclic hardening is observed in fatigue tests of modified asphalt controlled by low strain/stress level and it is not clear how the phenomenon affects the fatigue properties of binders. The special t... The phenomenon of cyclic hardening is observed in fatigue tests of modified asphalt controlled by low strain/stress level and it is not clear how the phenomenon affects the fatigue properties of binders. The special time weep tests were performed to investigate the point. Tests results indicate that the cyclic hardening is caused by the rearrangement of molecules in binders, and it can make the inner structure of binders getting stable and increase the fatigue properties of asphalt binders. But fatigue damage occurs when fatigue tests start, no matter the phenomenon of cyclic hardening happens or not. If the controlled load is low, the effect of rearrangement of molecules on material is beyond the effect of fatigue damage so that the cyclic hardening can be observed. When the load conditions get worse, the effect of slight fatigue damages produced in hardening stage will show. 展开更多
关键词 modified asphalt fatigue properties cyclic hardening
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Effects of Moisture on Rheological Properties of PAV Aged Asphalt Binders
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作者 Yu Xin Wei Jianming +3 位作者 Liu Yunxin Luo Yilin Wang Ying Yin Long 《China Petroleum Processing & Petrochemical Technology》 SCIE CAS 2013年第3期38-44,共7页
For the most coastal areas where the air is very humid,study on the effect of humidity on the performance of asphalt has its practical significance.However,limited research has been done to investigate the effect of h... For the most coastal areas where the air is very humid,study on the effect of humidity on the performance of asphalt has its practical significance.However,limited research has been done to investigate the effect of humidity on rheological properties of asphalt.In this study,asphalt binders were aged in the pressure aging vessel(PAV)under different conditions of moisture and aging time.Then Dynamic Shear Rheometer(DSR)and Bending Beam Rheometer(BBR)measurements were conducted to obtain the parameters relating to the complex modulus,phase angle,failure temperature,and creep stiffness.It was found that the low temperature performance decreased as evidenced by the results of the creep stiffness.While there were no significant differences among conditioned binders based on complex modulus G*and phase angleδ,it was found that theδvalue increased when the humidity increased from 0% to 80% ,and then theδvalue decreased when the humidity increased to 100% .In addition,the results of the failure temperature indicated that the humidity of 80% would be a critical point for the high temperature performance of the asphalt binders. 展开更多
关键词 humidity PAV aging asphalt binders rheological properties
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Enhancing the Mechanical Properties of Gap Graded Cold Asphalt Containing Cement Utilising By-Product Material
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作者 Abbas Al-Hdabi Al Nageim +1 位作者 Felecite Ruddock Linda Seton 《Journal of Civil Engineering and Architecture》 2013年第8期916-921,共6页
The little stiffness modulus, high voidage and long curing time has limited the use of CBEM's (cold bituminous emulsion mixtures) in road and highways to pavement experiencing low traffic. The aim of this study is ... The little stiffness modulus, high voidage and long curing time has limited the use of CBEM's (cold bituminous emulsion mixtures) in road and highways to pavement experiencing low traffic. The aim of this study is to improve the properties of gap graded CRA (cold rolled asphal0 containing OPC (ordinary portland cement) as filler by addition of a by-product material as an activator. OPC was added to the CRA as a replacement to the conventional mineral filler (0%-100%), while LJMUA (Liverpool John Moores University Activator) was added as an additive in the range from 0%-3% by total mass of aggregate. Laboratory tests included stiffness modulus and uniaxial creep test to assess the mechanical properties. The results have shown a considerable improvement in the mechanical properties from the addition of LJMUA to the CRA containing OPC especially for the early life stiffness modulus that is the main disadvantage of the cold mixtures. 展开更多
关键词 Stiffness modulus creep stiffness ordinary portland cement cold rolled asphalt.
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Analysis of Shop cold water stability of asphalt concrete composite
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作者 Weijie Li 《International Journal of Technology Management》 2015年第5期1-3,共3页
He cold asphalt concrete is laid composite that combines the advantages of rigid cement concrete and asphalt concrete flexible pavement materials and new waterproof materials, and it is also known as semi-rigid concre... He cold asphalt concrete is laid composite that combines the advantages of rigid cement concrete and asphalt concrete flexible pavement materials and new waterproof materials, and it is also known as semi-rigid concrete or semi-rigid waterproof concrete. Cold paved asphalt concrete composite retains the advantages of rigid and flexible waterproof material waterproof material which abandoned both of their inadequacies, is waterproof material with a wide range of space research and application prospects. This study immersion Marshall test and freeze-thaw split test two test methods for cold-laid asphalt concrete composite conducted a comprehensive analysis of the stability of the water; the highest draw AC1-6 AC-20 immersion Marshall stability and 20.59, respectively, by testing MPa and 19.96 Mpa, freeze-thaw splitting strength to reach the highest ratio of 91% and 93% respectively, the value specification can be met, and through the analysis of the test data to identify the content of the asphalt cement content and cold water laid asphalt compound affect the stability of the peak will occur, so that it can be combined with concrete interfacial adhesion studies to further the comprehensive and accurate assessment of water resistance of the material. 展开更多
关键词 cold-laid asphalt concrete composite water resistance strength test
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