[目的]探讨人单核细胞对煤焦沥青烟提取物(CTPE)处理人永生化支气管上皮细胞中c-Jun m RNA表达的影响。[方法]用3μg/m L CTPE刺激人永生化支气管上皮细胞(CTPE组),及人单核细胞共培养的人永生化支气管上皮细胞(CC组),设立二甲基亚砜对...[目的]探讨人单核细胞对煤焦沥青烟提取物(CTPE)处理人永生化支气管上皮细胞中c-Jun m RNA表达的影响。[方法]用3μg/m L CTPE刺激人永生化支气管上皮细胞(CTPE组),及人单核细胞共培养的人永生化支气管上皮细胞(CC组),设立二甲基亚砜对照组;分别收集各组1、5、10、15、20代细胞。将CC组第9代细胞分别常规培养至10代(CC10组)、15代(CC15组),加入100μg/m L肿瘤坏死因子-α中和抗体培养至15代(中和抗体组)。应用实时荧光定量聚合酶链式反应检测各组细胞中c-Jun m RNA的表达水平。[结果]15、20代CC组人永生化支气管上皮细胞c-Jun m RNA相对表达水平高于CTPE组和二甲基亚砜对照组(均P<0.05);中和抗体组c-Jun m RNA的相对表达水平高于CC10组,低于CC15组(均P<0.05)。[结论]人单核细胞可能通过肿瘤坏死因子-α调控CTPE诱导人永生化支气管上皮细胞c-Jun m RNA的表达。展开更多
A new testing procedure to estimate the low-temperature stiffness of the reclaimed asphalt pavement （RAP） binder was developed. In the testing procedure, the SuperpaveTM Bending Beam Rheometer （BBR） with special m...A new testing procedure to estimate the low-temperature stiffness of the reclaimed asphalt pavement （RAP） binder was developed. In the testing procedure, the SuperpaveTM Bending Beam Rheometer （BBR） with special modifications and binder blending charts by Asphalt Institute were utilized. Modifications involved the development of a new kind of sample mold and different testing parameters were made to BBR testing procedure to capture the theological properties of bitumen mortars produced by mixing fresh binder with fine RAP materials or RAP aggregate. The stiffness relationship between binder and bitumen mortar was established based on the BBR test results. The blended binder stiffness in bitumen RAP mortar was estimated from the RAP mortar stiffness based on the binder-mortar relationship. And finally, the RAP binder stiffness was estimated from the blended binder and fresh binder stiffness based on the blending charts by Asphalt Institute. The results indicate that the new procedure can capture the rheological properties of bitumen mortar and can be used to estimate the low temperature stiffness of RAP binder without binder extraction and/or any chemical treatments.展开更多
Coal-tar pitch(CP)is a promising carbon raw material for producing needle coke,carbon fiber etc.During processing,the H/C ratio,ash content,and quinoline insoluble(QI)in the CP are the key factors that influence the m...Coal-tar pitch(CP)is a promising carbon raw material for producing needle coke,carbon fiber etc.During processing,the H/C ratio,ash content,and quinoline insoluble(QI)in the CP are the key factors that influence the material preparation.In this study,NMP was selected to extract CP first;then[BMIM]Cl/NMP mixed solvent was used;and finally a series of ionic liquids(ILs)mixtures with NMP were developed for the extraction of CP to obtain the refined pitch.The extracts were analyzed via elemental analysis,TGA,FT-IR,and 13C-NMR.Results indicate that different NMP/IL mass ratios or different kinds of ILs have impact on the extraction yield.The relationship of the hydrogen to carbon(H/C)ratio changed with different solvents and QI extracts were obtained.Results showed that the H/C ratios changed little between NMP extracts and could be adjusted by changing the NMP/ILs mass ratio or using different ILs.The extracts are suitable for preparation mesophase pitch because of no ash content,low QI,and appropriate H/C ratios.As a result,NMP can be used to refine pitch.In addition,[BMIM]Cl is good mixed with NMP for CP extraction,because it can obtain a relatively high yield under the same extraction conditions.展开更多
文摘[目的]探讨人单核细胞对煤焦沥青烟提取物(CTPE)处理人永生化支气管上皮细胞中c-Jun m RNA表达的影响。[方法]用3μg/m L CTPE刺激人永生化支气管上皮细胞(CTPE组),及人单核细胞共培养的人永生化支气管上皮细胞(CC组),设立二甲基亚砜对照组;分别收集各组1、5、10、15、20代细胞。将CC组第9代细胞分别常规培养至10代(CC10组)、15代(CC15组),加入100μg/m L肿瘤坏死因子-α中和抗体培养至15代(中和抗体组)。应用实时荧光定量聚合酶链式反应检测各组细胞中c-Jun m RNA的表达水平。[结果]15、20代CC组人永生化支气管上皮细胞c-Jun m RNA相对表达水平高于CTPE组和二甲基亚砜对照组(均P<0.05);中和抗体组c-Jun m RNA的相对表达水平高于CC10组,低于CC15组(均P<0.05)。[结论]人单核细胞可能通过肿瘤坏死因子-α调控CTPE诱导人永生化支气管上皮细胞c-Jun m RNA的表达。
基金Project(200831800044) supported by the Ministry of Communication of ChinaProject(50878054) supported by the National Natural Science Foundation of ChinaProject(06Y31) supported by the Department of Communication of Zhejiang Province,China
文摘A new testing procedure to estimate the low-temperature stiffness of the reclaimed asphalt pavement （RAP） binder was developed. In the testing procedure, the SuperpaveTM Bending Beam Rheometer （BBR） with special modifications and binder blending charts by Asphalt Institute were utilized. Modifications involved the development of a new kind of sample mold and different testing parameters were made to BBR testing procedure to capture the theological properties of bitumen mortars produced by mixing fresh binder with fine RAP materials or RAP aggregate. The stiffness relationship between binder and bitumen mortar was established based on the BBR test results. The blended binder stiffness in bitumen RAP mortar was estimated from the RAP mortar stiffness based on the binder-mortar relationship. And finally, the RAP binder stiffness was estimated from the blended binder and fresh binder stiffness based on the blending charts by Asphalt Institute. The results indicate that the new procedure can capture the rheological properties of bitumen mortar and can be used to estimate the low temperature stiffness of RAP binder without binder extraction and/or any chemical treatments.
基金supported by the National Basic Research Program of China(2012CB214905)the National Natural Science Foundation of China(21076113)
文摘Coal-tar pitch(CP)is a promising carbon raw material for producing needle coke,carbon fiber etc.During processing,the H/C ratio,ash content,and quinoline insoluble(QI)in the CP are the key factors that influence the material preparation.In this study,NMP was selected to extract CP first;then[BMIM]Cl/NMP mixed solvent was used;and finally a series of ionic liquids(ILs)mixtures with NMP were developed for the extraction of CP to obtain the refined pitch.The extracts were analyzed via elemental analysis,TGA,FT-IR,and 13C-NMR.Results indicate that different NMP/IL mass ratios or different kinds of ILs have impact on the extraction yield.The relationship of the hydrogen to carbon(H/C)ratio changed with different solvents and QI extracts were obtained.Results showed that the H/C ratios changed little between NMP extracts and could be adjusted by changing the NMP/ILs mass ratio or using different ILs.The extracts are suitable for preparation mesophase pitch because of no ash content,low QI,and appropriate H/C ratios.As a result,NMP can be used to refine pitch.In addition,[BMIM]Cl is good mixed with NMP for CP extraction,because it can obtain a relatively high yield under the same extraction conditions.
