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农杆菌介导油菜油体基因与碱性成纤维细胞生长因子融合基因转化油菜研究 被引量:7
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作者 徐岩 肖艳双 +4 位作者 杜金霞 汪洪 郑伟 李营 庞实锋 《安徽农业科学》 CAS 北大核心 2009年第27期12936-12939,共4页
[目的]利用植物转基因技术,生产药用蛋白。[方法]以带柄子叶作为转化受体,利用根癌农杆菌介导法将油菜油体基因与bFGF的融合基因转入油菜中,在获得潮霉素(Hyg)抗性植株的同时,对油菜的再生条件进行优化,并对获得的抗性苗进行PCR和South... [目的]利用植物转基因技术,生产药用蛋白。[方法]以带柄子叶作为转化受体,利用根癌农杆菌介导法将油菜油体基因与bFGF的融合基因转入油菜中,在获得潮霉素(Hyg)抗性植株的同时,对油菜的再生条件进行优化,并对获得的抗性苗进行PCR和Southern检测。[结果]油菜最佳转化再生条件为外植体预培养2 d,共培养3 d,菌液浓度OD值为0.3,侵染时间5 min。[结论]该研究中,融合基因已成功整合到油菜基因组中,为以后转基因植物种子的蛋白提取、分离和纯化奠定了基础。 展开更多
关键词 碱性成纤维细胞生长因子(bFGF) 植物生物反应器 油体系统 根癌农杆菌
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Operation and Optimization of Residue Hydrotreating Unit Using New Catalyst System 被引量:3
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作者 Yin Zhaolin 《China Petroleum Processing & Petrochemical Technology》 SCIE CAS 2012年第3期50-58,共9页
This article has summarized the optimized measures relating to the loading of catalyst,and the sixth operating cycle of the residue hydrotreating unit at SINOPEC's Maoming Branch Company,and made a detailed compar... This article has summarized the optimized measures relating to the loading of catalyst,and the sixth operating cycle of the residue hydrotreating unit at SINOPEC's Maoming Branch Company,and made a detailed comparison on the impurities removal rate,hydrogen consumption and energy consumption of the sixth operating cycle with those achieved by the previous five cycles.Test results have revealed that the second-generation RHT series novel residue hydrotreating catalysts featured high activity,good stability,and long operating cycle and could remarkably reduce the hydrogen consumption and energy consumption of process unit.The hydrotreated AR product,having low Conradson carbon residue,low sulfur content,low metal content,high content of saturated hydrocarbons,and low content of asphaltenes and resins,is an excellent FCC feed.Judging from their overall property the second-generation RHT series of residue hydrotreating catalysts used in the sixth operating cycle have commanded a leading position among other catalysts used in previous operating cycles. 展开更多
关键词 residue hydrotreating CATALYST performance of unit OPTIMIZATION
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Landslide Susceptibility Assessment of the Youfang Catchment using Logistic Regression 被引量:6
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作者 BAI Shi-biao LU Ping WANG Jian 《Journal of Mountain Science》 SCIE CSCD 2015年第4期816-827,共12页
A detailed landslide susceptibility map was produced in the Youfang catchment using logistic regression method with datasets developed for a geographic information system(GIS).Known as one of the most landslide-prone ... A detailed landslide susceptibility map was produced in the Youfang catchment using logistic regression method with datasets developed for a geographic information system(GIS).Known as one of the most landslide-prone areas in China, the Youfang catchment of Longnan mountain region,which lies in the transitional area among QinghaiTibet Plateau, loess Plateau and Sichuan Basin, was selected as a representative case to evaluate the frequency and distribution of landslides.Statistical relationships for landslide susceptibility assessment were developed using landslide and landslide causative factor databases.Logistic regression(LR)was used to create the landslide susceptibility maps based on a series of available data sources: landslide inventory; distance to drainage systems, faults and roads; slope angle and aspect; topographic elevation and topographical wetness index, and land use.The quality of the landslide susceptibility map produced in this paper was validated and the result can be used fordesigning protective and mitigation measures against landslide hazards.The landslide susceptibility map is expected to provide a fundamental tool for landslide hazards assessment and risk management in the Youfang catchment. 展开更多
关键词 LANDSLIDE Susceptibility map Logistic regression Geographic Information System(GIS) Youfang catchment
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Plant as Bioreactor for Producing Heterologous Proteins
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作者 YANG Jing XIONG Li-dong +3 位作者 LI Tian-hang GUO Yong-xin LI Hai-yan LI Xiao-kun 《Chinese Journal of Biomedical Engineering(English Edition)》 2010年第4期156-173,共18页
Using plants to produce heterologous proteins makes it very attractive due to the potentially low costs. Using this procedure it is possible to produce medicinal protein for clinical applications with the plants biore... Using plants to produce heterologous proteins makes it very attractive due to the potentially low costs. Using this procedure it is possible to produce medicinal protein for clinical applications with the plants bioreactors increasing gradually. The paper proposes the five major systems of the plant bioreactor as well as their advantage and disadvantage and the development of each system. Focuses on the five major systems of the plant bioreactor to produce vaccines, antibodies and medical protein and the research achievement at the present stage and the research on my laboratory. The key technology research of plant bioreactor such as new genes, new biological components, new technologies and new research methods related with plant bioreactor offer a work foundation for a long-term development in future. 展开更多
关键词 BIOREACTOR in planta oil body CHLOROPLAST suspension system plantvirus heterologous protein
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Prospect of Mid-long Term Oil & Gas System Reform in China
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作者 Guo Jiaofeng 《China Oil & Gas》 CAS 2015年第2期10-15,共6页
International oil keeps low-price running after crash, and China deepens reformm domestically. Using this opportunity, China lays the same emphasis on investment and trade, on onshore and offshore transportation, give... International oil keeps low-price running after crash, and China deepens reformm domestically. Using this opportunity, China lays the same emphasis on investment and trade, on onshore and offshore transportation, gives full play to the important role of market in resource distribution, speeds up oil and gas system reform to guarantee clean, e fficienL safe and steady energy supply and long-term demand for energy. We systematically and thoroughly established legal system for oil and gas domains, reformed management system for energy domain, re&treed regulatory domain, built modern market entity and multi-level market system, and improved oil and gas pricing mechanism, etc. 展开更多
关键词 oil and gas MARKETIZATION system reform strategy energy China
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