以外来疫病——油菜茎基溃疡病为例,提出一种未成功入侵外来生物导致的潜在损失评估方法,根据GARP生态位模型ArcGIS栅格数据图,通过掩膜工具Extract By Mask,提取出每个适生类型在中国各省份的分布面积。构建潜在损失估算模型,从潜在直...以外来疫病——油菜茎基溃疡病为例,提出一种未成功入侵外来生物导致的潜在损失评估方法,根据GARP生态位模型ArcGIS栅格数据图,通过掩膜工具Extract By Mask,提取出每个适生类型在中国各省份的分布面积。构建潜在损失估算模型,从潜在直接经济损失(农产品产值损失、农副食品加工业损失和防控费用)、潜在间接经济损失(油菜旅游产业)和潜在非经济损失(生态系统功能损失)3个方面构建外来入侵生物损失估算体系。研究发现油菜茎基溃疡病入侵的潜在损失总额高达96.05亿元,其中,潜在直接经济损失、潜在间接经济损失和潜在非经济损失分别占潜在损失总量的54.9%、36.0%和9.1%。动植物检疫在防范外来生物入侵中发挥着重要作用。展开更多
41 fungal isolates with similar morphological characteristics to Leptosphaeria maculans were obtained by the deep-freezing filter paper method from 2100 seeds of Brassica napus imported from Australia.The isolate 8129...41 fungal isolates with similar morphological characteristics to Leptosphaeria maculans were obtained by the deep-freezing filter paper method from 2100 seeds of Brassica napus imported from Australia.The isolate 8129-5 showed a slower growth on PDA at 20℃with growth rate of 2.8 mm/day.The colonies on PDA at 20℃ had an irregular or regular margin with white or grayish white compact aerial mycelium.No diffusible pigment was produced on PDA at 31℃ or in liquid Czapek-Dox media at 20℃.PCR detection showed that the isolate 8129-5 could be amplified by L.maculans-specific primers LmacF/LmacR and got expected product of 331 bp.The sequence analysis revealed that the ITS sequence of isolate 8129-5 had 99.8% identity with L.maculans.Pathogenicity of the isolate 8129-5 was confirmed on cotyledons of rape seed by artificial inoculation compared with typical symptom of L.maculans.Based on the morphological characteristics,PCR detection and the result of pathogenicity test,the isolate 8129-5 was identified as L.maculans.展开更多
文摘以外来疫病——油菜茎基溃疡病为例,提出一种未成功入侵外来生物导致的潜在损失评估方法,根据GARP生态位模型ArcGIS栅格数据图,通过掩膜工具Extract By Mask,提取出每个适生类型在中国各省份的分布面积。构建潜在损失估算模型,从潜在直接经济损失(农产品产值损失、农副食品加工业损失和防控费用)、潜在间接经济损失(油菜旅游产业)和潜在非经济损失(生态系统功能损失)3个方面构建外来入侵生物损失估算体系。研究发现油菜茎基溃疡病入侵的潜在损失总额高达96.05亿元,其中,潜在直接经济损失、潜在间接经济损失和潜在非经济损失分别占潜在损失总量的54.9%、36.0%和9.1%。动植物检疫在防范外来生物入侵中发挥着重要作用。
文摘41 fungal isolates with similar morphological characteristics to Leptosphaeria maculans were obtained by the deep-freezing filter paper method from 2100 seeds of Brassica napus imported from Australia.The isolate 8129-5 showed a slower growth on PDA at 20℃with growth rate of 2.8 mm/day.The colonies on PDA at 20℃ had an irregular or regular margin with white or grayish white compact aerial mycelium.No diffusible pigment was produced on PDA at 31℃ or in liquid Czapek-Dox media at 20℃.PCR detection showed that the isolate 8129-5 could be amplified by L.maculans-specific primers LmacF/LmacR and got expected product of 331 bp.The sequence analysis revealed that the ITS sequence of isolate 8129-5 had 99.8% identity with L.maculans.Pathogenicity of the isolate 8129-5 was confirmed on cotyledons of rape seed by artificial inoculation compared with typical symptom of L.maculans.Based on the morphological characteristics,PCR detection and the result of pathogenicity test,the isolate 8129-5 was identified as L.maculans.
