With excessive utilization of antibiotics in recent years,bacterial drug resistance problem is serious increasingly,and it is more and more difficult to develop anti-infective drug,while it does not have these problem...With excessive utilization of antibiotics in recent years,bacterial drug resistance problem is serious increasingly,and it is more and more difficult to develop anti-infective drug,while it does not have these problems to use phage controlling disease.Phage is a kind of prokaryotic virus,widely exists in the nature and includes bacteriophage,cyanophage and actinophage.Due to its potential of replacing antibiotics to treat disease,phage receives more and more attention.In this paper,based on development status of phage research at home and abroad,discovery process,naming method and classification basis of phage are introduced comprehensively,and advantages and limitations of phage applying in prevention and control of bacterial diseases are analyzed.We introduce application status of phage in human medicine,prevention and control of diseases for terrestrial animals and aquaculture,and the effects of phage in sewage treatment,prevention and control of microbial contamination of food and detection technology,and point out the shortages of phage in the above application.Meanwhile,we also discuss application prospects of phage in disease prevention and control,environmental protection and food safety.展开更多
Stem rust race Ug99, also designated TTKSK （Puccinia graminis f. sp. tritici） cause stem or black rust, which is a serious disease of wheat worldwide. Field experiments were conducted at two sites during 2008 and 20...Stem rust race Ug99, also designated TTKSK （Puccinia graminis f. sp. tritici） cause stem or black rust, which is a serious disease of wheat worldwide. Field experiments were conducted at two sites during 2008 and 2009 growing seasons to evaluate the effectiveness of two new foliar fungicides： viz. Nativo 300 SC （trifloxystrobin 100 g L^-1 ＋ tebuconazole 200 g L^-1） and Prosaro 250 EC （prothioconazole 125 g L^-1 ＋ tebuconazole 125 g L^-1）, in controlling stem rust on susceptible wheat cultivar Duma. AmistarXtra 280 SC （azoxystrobin 200 g L^-1 ＋ cyproconazole 80 g L^-1） and Folicur 250 EC （tebuconazole） were used as checks. The treatment at each site and year included non-treated control and two spray applications of the fungicides at growth stages （GS） 55 （heading） and 65 （flowering）. Stem rust severities were scored using the modified Cobb scale at 14-day intervals after application. The data were used to calculate mean rust severity （MRS）. Stem rust epidemics were severe at KARI-Njoro in 2008 and the treatment effects on stem rust severities, grain yield and 1,000 kernel weights were significant at both KARI-Njoro and Mau-Narok sites. The fungicide treatments, significantly （P _〈 0.05） reduced stem rust severity, increased grain yield and 1,000 kernel weight of the susceptible wheat cultivar Duma compared to the non-treated control. Both fungicides： Nativo 300 SC and Prosaro 250 EC applied at the rate of 1.0 L hat were recommended for commercial use.展开更多
基金Supported by Special Fund for Scientific Research in Marine Public Welfare Industry(201405003)
文摘With excessive utilization of antibiotics in recent years,bacterial drug resistance problem is serious increasingly,and it is more and more difficult to develop anti-infective drug,while it does not have these problems to use phage controlling disease.Phage is a kind of prokaryotic virus,widely exists in the nature and includes bacteriophage,cyanophage and actinophage.Due to its potential of replacing antibiotics to treat disease,phage receives more and more attention.In this paper,based on development status of phage research at home and abroad,discovery process,naming method and classification basis of phage are introduced comprehensively,and advantages and limitations of phage applying in prevention and control of bacterial diseases are analyzed.We introduce application status of phage in human medicine,prevention and control of diseases for terrestrial animals and aquaculture,and the effects of phage in sewage treatment,prevention and control of microbial contamination of food and detection technology,and point out the shortages of phage in the above application.Meanwhile,we also discuss application prospects of phage in disease prevention and control,environmental protection and food safety.
文摘Stem rust race Ug99, also designated TTKSK （Puccinia graminis f. sp. tritici） cause stem or black rust, which is a serious disease of wheat worldwide. Field experiments were conducted at two sites during 2008 and 2009 growing seasons to evaluate the effectiveness of two new foliar fungicides： viz. Nativo 300 SC （trifloxystrobin 100 g L^-1 ＋ tebuconazole 200 g L^-1） and Prosaro 250 EC （prothioconazole 125 g L^-1 ＋ tebuconazole 125 g L^-1）, in controlling stem rust on susceptible wheat cultivar Duma. AmistarXtra 280 SC （azoxystrobin 200 g L^-1 ＋ cyproconazole 80 g L^-1） and Folicur 250 EC （tebuconazole） were used as checks. The treatment at each site and year included non-treated control and two spray applications of the fungicides at growth stages （GS） 55 （heading） and 65 （flowering）. Stem rust severities were scored using the modified Cobb scale at 14-day intervals after application. The data were used to calculate mean rust severity （MRS）. Stem rust epidemics were severe at KARI-Njoro in 2008 and the treatment effects on stem rust severities, grain yield and 1,000 kernel weights were significant at both KARI-Njoro and Mau-Narok sites. The fungicide treatments, significantly （P _〈 0.05） reduced stem rust severity, increased grain yield and 1,000 kernel weight of the susceptible wheat cultivar Duma compared to the non-treated control. Both fungicides： Nativo 300 SC and Prosaro 250 EC applied at the rate of 1.0 L hat were recommended for commercial use.
