BACKGROUND:Adeno-associated virus(AAV)gene therapy has been proven to be reliable and safe for the treatment of osteoarthritis in recent years.However,given the complexity of osteoarthritis pathogenesis,single gene ma...BACKGROUND:Adeno-associated virus(AAV)gene therapy has been proven to be reliable and safe for the treatment of osteoarthritis in recent years.However,given the complexity of osteoarthritis pathogenesis,single gene manipulation for the treatment of osteoarthritis may not produce satisfactory results.Previous studies have shown that nuclear factorκB could promote the inflammatory pathway in osteoarthritic chondrocytes,and bone morphogenetic protein 4(BMP4)could promote cartilage regeneration.OBJECTIVE:To test whether combined application of AAV-p65shRNA and AAV-BMP4 will yield the synergistic effect on chondrocytes regeneration and osteoarthritis treatment.METHODS:Viral particles containing AAV-p65-shRNA and AAV-BMP4 were prepared.Their efficacy in inhibiting inflammation in chondrocytes and promoting chondrogenesis was assessed in vitro and in vivo by transfecting AAV-p65-shRNA or AAV-BMP4 into cells.The experiments were divided into five groups:PBS group;osteoarthritis group;AAV-BMP4 group;AAV-p65shRNA group;and BMP4-p65shRNA 1:1 group.Samples were collected at 4,12,and 24 weeks postoperatively.Tissue staining,including safranin O and Alcian blue,was applied after collecting articular tissue.Then,the optimal ratio between the two types of transfected viral particles was further investigated to improve the chondrogenic potential of mixed cells in vivo.RESULTS AND CONCLUSION:The combined application of AAV-p65shRNA and AAV-BMP4 together showed a synergistic effect on cartilage regeneration and osteoarthritis treatment.Mixed cells transfected with AAV-p65shRNA and AAV-BMP4 at a 1:1 ratio produced the most extracellular matrix synthesis(P<0.05).In vivo results also revealed that the combination of the two viruses had the highest regenerative potential for osteoarthritic cartilage(P<0.05).In the present study,we also discovered that the combined therapy had the maximum effect when the two viruses were administered in equal proportions.Decreasing either p65shRNA or BMP4 transfected cells resulted in less collagen II synthesis.This implies that inhibiting inflammation by p65shRNA and promoting regeneration by BMP4 are equally important for osteoarthritis treatment.These findings provide a new strategy for the treatment of early osteoarthritis by simultaneously inhibiting cartilage inflammation and promoting cartilage repair.展开更多
目的 :探讨用PHENIX USB 4型神经肌肉刺激治疗仪对接受人流手术的女性进行治疗的效果。方法 :将2017年7月至2018年7月期间在罗定市人民医院接受人工流产手术的100例意外妊娠女性作为研究对象。将这100例女性随机分为观察组和对照组,每...目的 :探讨用PHENIX USB 4型神经肌肉刺激治疗仪对接受人流手术的女性进行治疗的效果。方法 :将2017年7月至2018年7月期间在罗定市人民医院接受人工流产手术的100例意外妊娠女性作为研究对象。将这100例女性随机分为观察组和对照组,每组各有50例女性。为对照组研究对象使用产复康颗粒进行治疗,为观察组研究对象使用PHENIX USB 4型神经肌肉刺激治疗仪进行治疗。然后,对比两组研究对象术后疼痛的程度、子宫复旧的情况及其并发症的发生情况。结果:与对照组研究对象相比,在观察组研究对象中,术后疼痛程度为无痛的女性所占的比例较高,术后疼痛程度为轻度疼痛和中度疼痛的女性所占的比例均较低,对其进行子宫复旧治疗的总有效率较高,其宫腔瘀血、宫腔内残留及宫腔内凝血块滞留的发生率均较低(P <0.05)。结论 :用PHENIX USB 4型神经肌肉刺激治疗仪对接受人流手术的女性进行治疗的效果显著,可有效地缓解其术后疼痛感,促进其子宫复旧,降低其术后并发症的发生率。展开更多
文摘BACKGROUND:Adeno-associated virus(AAV)gene therapy has been proven to be reliable and safe for the treatment of osteoarthritis in recent years.However,given the complexity of osteoarthritis pathogenesis,single gene manipulation for the treatment of osteoarthritis may not produce satisfactory results.Previous studies have shown that nuclear factorκB could promote the inflammatory pathway in osteoarthritic chondrocytes,and bone morphogenetic protein 4(BMP4)could promote cartilage regeneration.OBJECTIVE:To test whether combined application of AAV-p65shRNA and AAV-BMP4 will yield the synergistic effect on chondrocytes regeneration and osteoarthritis treatment.METHODS:Viral particles containing AAV-p65-shRNA and AAV-BMP4 were prepared.Their efficacy in inhibiting inflammation in chondrocytes and promoting chondrogenesis was assessed in vitro and in vivo by transfecting AAV-p65-shRNA or AAV-BMP4 into cells.The experiments were divided into five groups:PBS group;osteoarthritis group;AAV-BMP4 group;AAV-p65shRNA group;and BMP4-p65shRNA 1:1 group.Samples were collected at 4,12,and 24 weeks postoperatively.Tissue staining,including safranin O and Alcian blue,was applied after collecting articular tissue.Then,the optimal ratio between the two types of transfected viral particles was further investigated to improve the chondrogenic potential of mixed cells in vivo.RESULTS AND CONCLUSION:The combined application of AAV-p65shRNA and AAV-BMP4 together showed a synergistic effect on cartilage regeneration and osteoarthritis treatment.Mixed cells transfected with AAV-p65shRNA and AAV-BMP4 at a 1:1 ratio produced the most extracellular matrix synthesis(P<0.05).In vivo results also revealed that the combination of the two viruses had the highest regenerative potential for osteoarthritic cartilage(P<0.05).In the present study,we also discovered that the combined therapy had the maximum effect when the two viruses were administered in equal proportions.Decreasing either p65shRNA or BMP4 transfected cells resulted in less collagen II synthesis.This implies that inhibiting inflammation by p65shRNA and promoting regeneration by BMP4 are equally important for osteoarthritis treatment.These findings provide a new strategy for the treatment of early osteoarthritis by simultaneously inhibiting cartilage inflammation and promoting cartilage repair.
文摘目的 :探讨用PHENIX USB 4型神经肌肉刺激治疗仪对接受人流手术的女性进行治疗的效果。方法 :将2017年7月至2018年7月期间在罗定市人民医院接受人工流产手术的100例意外妊娠女性作为研究对象。将这100例女性随机分为观察组和对照组,每组各有50例女性。为对照组研究对象使用产复康颗粒进行治疗,为观察组研究对象使用PHENIX USB 4型神经肌肉刺激治疗仪进行治疗。然后,对比两组研究对象术后疼痛的程度、子宫复旧的情况及其并发症的发生情况。结果:与对照组研究对象相比,在观察组研究对象中,术后疼痛程度为无痛的女性所占的比例较高,术后疼痛程度为轻度疼痛和中度疼痛的女性所占的比例均较低,对其进行子宫复旧治疗的总有效率较高,其宫腔瘀血、宫腔内残留及宫腔内凝血块滞留的发生率均较低(P <0.05)。结论 :用PHENIX USB 4型神经肌肉刺激治疗仪对接受人流手术的女性进行治疗的效果显著,可有效地缓解其术后疼痛感,促进其子宫复旧,降低其术后并发症的发生率。
