AIM: To evaluate the effect of rosiglitazone in a murine model of liver fibrosis induced by Schistosoma japonicum infection. METHODS: A total of 50 mice were randomly and averagely divided into groups A, B, C, D and E...AIM: To evaluate the effect of rosiglitazone in a murine model of liver fibrosis induced by Schistosoma japonicum infection. METHODS: A total of 50 mice were randomly and averagely divided into groups A, B, C, D and E. The mice in group A served as normal controls, while those in the other four groups were infected with Schistosoma japonicum to induce the model of liver fibrosis. Besides, the mice in groups C, D and E were treated with praziquantel, rosiglitazone and praziquantel plus rosiglitazone, respectively. NF-κB binding activity and expression of PPARγ-mRNA were determined by Western blot assay and real-time quantitative PCR. Radioimmunonassay technique was used to detect the serum content changes of TNF-α and IL-6. Histological specimens were stained with HE. Expression of TGF-β1, a-smooth muscle actin and type ?Ⅰ?and type Ⅲ collagen was detected by immunohistochemistry and multimedia color pathographic analysis system. RESULTS: Inflammation and fibrosis in the rosiglitazone plus praziquantel treatment group (group E) were lightest among the mice infected with Schistosoma (P < 0.05). To further explore the mechanism of rosiglitazone action, we found that rosiglitazone can significantly increase the expression of PPARγ [E: -18.212 ± (-3.909) vs B: -27.315 ± (-6.348) and C: -25.647 ± (-5.694), P < 0.05],reduce the NF-κB binding activity (E: 88.89 ± 19.34 vs B: 141.11 ± 15.37, C: 112.89 ± 20.17 and D: 108.89 ± 20.47, P < 0.05), and lower the serum level of TNF-α (E: 1.613 ± 0.420 ng/mL vs B: 2.892 ± 0.587 ng/mL, C: 2.346 ± 0.371 ng/mL and D: 2.160 ± 0.395 ng/mL, P < 0.05) and IL-6 (E: 0.106 ± 0.021 ng/mL vs B: 0.140 ± 0.031 ng/mL and C: 0.137 ± 0.027 ng/mL, P < 0.05) in mice with liver fibrosis. Rosiglitazone can also substantially reduce the hepatic expression of TGF-β1, α-SMA type Ⅰand type Ⅲ collagen in mice with liver fibrosis. CONCLUSION: The activation of PPARγ by its ligand can retard liver fibrosis and suggest the use of rosiglitazone for the treatment of liver fibrosis due to Schistosoma japonicum infection.展开更多
Opisthorchiasis caused by Opisthorchis viverrini （Oviverrini） remains a major public health problem in many parts of Southeast Asia including Thailand, Lao PDR, Vietnam and Cambodia. The infection is associated with...Opisthorchiasis caused by Opisthorchis viverrini （Oviverrini） remains a major public health problem in many parts of Southeast Asia including Thailand, Lao PDR, Vietnam and Cambodia. The infection is associated with a number of hepatobUiary diseases, including cholangitis, obstructive jaundice, hepatomegaly, cholecystitis, cholelithiasis and cholangiocarcinoma. The liver fluke infection was induced by eating raw or uncooked fish products that is the tradition and popular in the northeastern and northern region, particularly in rural areas of Thailand. Health education programs to prevent and control opisthorchiasis are still required in high-risk areas.展开更多
Training and demonstrations as a means of post harvest technology transfer at farm level were conducted in 5 divisions of Kitui district. The aim was to reinforce farmers＇ knowledge about storage and help them select...Training and demonstrations as a means of post harvest technology transfer at farm level were conducted in 5 divisions of Kitui district. The aim was to reinforce farmers＇ knowledge about storage and help them select proper application methods of pest control suitable for their storage. A total of 163 participants were trained in various storage pest management topics. Before each demonstration, farmers listed their common methods of mixing grain with chemical dusts. Shovel mixing on tarpaulin or wheelbarrows and in-bag stick mixing were evaluated alongside the research way of using the shovel for grain mixing and the ＂fuffle＂, a more efficient device for mixing grain with chemical dusts. Only the 1.6% pirimiphos methyl/0.3% permethrin （Actellic super dust） was used and the treated and untreated controls were stored on site in 90 kg bags. After two months, damage and live infestation were assessed in 1 kg samples drawn from each mixing method. Despite lack of uniformity of grain due to different sources, farmers were able to identify the mixing methods which gave better results. KARl methods appeared better and the fhffle was rated as an effective and labour saving tool and farmers were keen to have it fabricated by local artisans.展开更多
Schistosomiasis, an important parasitic disease, is severely harmful to human heath in China . The beach along the Yangtze River is a important habitat of snail that is Schistosomiasis＇s only intermediate host. pro...Schistosomiasis, an important parasitic disease, is severely harmful to human heath in China . The beach along the Yangtze River is a important habitat of snail that is Schistosomiasis＇s only intermediate host. program of afforesting for wiping out snail（Oncomelania hupensisi） and Schistosomiasis, comprehensive administration and exploitation of the three kinds of beaches proposed by Peng Zhenhna and Jiang Zehui is a pioneering.work hased on the ecological and economic theory. The development process of forestry for snail control and Schistosomiasis prevention is reviewed, and mechanisms and major afforesting techniques are summarized in this paper.展开更多
文摘AIM: To evaluate the effect of rosiglitazone in a murine model of liver fibrosis induced by Schistosoma japonicum infection. METHODS: A total of 50 mice were randomly and averagely divided into groups A, B, C, D and E. The mice in group A served as normal controls, while those in the other four groups were infected with Schistosoma japonicum to induce the model of liver fibrosis. Besides, the mice in groups C, D and E were treated with praziquantel, rosiglitazone and praziquantel plus rosiglitazone, respectively. NF-κB binding activity and expression of PPARγ-mRNA were determined by Western blot assay and real-time quantitative PCR. Radioimmunonassay technique was used to detect the serum content changes of TNF-α and IL-6. Histological specimens were stained with HE. Expression of TGF-β1, a-smooth muscle actin and type ?Ⅰ?and type Ⅲ collagen was detected by immunohistochemistry and multimedia color pathographic analysis system. RESULTS: Inflammation and fibrosis in the rosiglitazone plus praziquantel treatment group (group E) were lightest among the mice infected with Schistosoma (P < 0.05). To further explore the mechanism of rosiglitazone action, we found that rosiglitazone can significantly increase the expression of PPARγ [E: -18.212 ± (-3.909) vs B: -27.315 ± (-6.348) and C: -25.647 ± (-5.694), P < 0.05],reduce the NF-κB binding activity (E: 88.89 ± 19.34 vs B: 141.11 ± 15.37, C: 112.89 ± 20.17 and D: 108.89 ± 20.47, P < 0.05), and lower the serum level of TNF-α (E: 1.613 ± 0.420 ng/mL vs B: 2.892 ± 0.587 ng/mL, C: 2.346 ± 0.371 ng/mL and D: 2.160 ± 0.395 ng/mL, P < 0.05) and IL-6 (E: 0.106 ± 0.021 ng/mL vs B: 0.140 ± 0.031 ng/mL and C: 0.137 ± 0.027 ng/mL, P < 0.05) in mice with liver fibrosis. Rosiglitazone can also substantially reduce the hepatic expression of TGF-β1, α-SMA type Ⅰand type Ⅲ collagen in mice with liver fibrosis. CONCLUSION: The activation of PPARγ by its ligand can retard liver fibrosis and suggest the use of rosiglitazone for the treatment of liver fibrosis due to Schistosoma japonicum infection.
文摘Opisthorchiasis caused by Opisthorchis viverrini （Oviverrini） remains a major public health problem in many parts of Southeast Asia including Thailand, Lao PDR, Vietnam and Cambodia. The infection is associated with a number of hepatobUiary diseases, including cholangitis, obstructive jaundice, hepatomegaly, cholecystitis, cholelithiasis and cholangiocarcinoma. The liver fluke infection was induced by eating raw or uncooked fish products that is the tradition and popular in the northeastern and northern region, particularly in rural areas of Thailand. Health education programs to prevent and control opisthorchiasis are still required in high-risk areas.
文摘Training and demonstrations as a means of post harvest technology transfer at farm level were conducted in 5 divisions of Kitui district. The aim was to reinforce farmers＇ knowledge about storage and help them select proper application methods of pest control suitable for their storage. A total of 163 participants were trained in various storage pest management topics. Before each demonstration, farmers listed their common methods of mixing grain with chemical dusts. Shovel mixing on tarpaulin or wheelbarrows and in-bag stick mixing were evaluated alongside the research way of using the shovel for grain mixing and the ＂fuffle＂, a more efficient device for mixing grain with chemical dusts. Only the 1.6% pirimiphos methyl/0.3% permethrin （Actellic super dust） was used and the treated and untreated controls were stored on site in 90 kg bags. After two months, damage and live infestation were assessed in 1 kg samples drawn from each mixing method. Despite lack of uniformity of grain due to different sources, farmers were able to identify the mixing methods which gave better results. KARl methods appeared better and the fhffle was rated as an effective and labour saving tool and farmers were keen to have it fabricated by local artisans.
文摘Schistosomiasis, an important parasitic disease, is severely harmful to human heath in China . The beach along the Yangtze River is a important habitat of snail that is Schistosomiasis＇s only intermediate host. program of afforesting for wiping out snail（Oncomelania hupensisi） and Schistosomiasis, comprehensive administration and exploitation of the three kinds of beaches proposed by Peng Zhenhna and Jiang Zehui is a pioneering.work hased on the ecological and economic theory. The development process of forestry for snail control and Schistosomiasis prevention is reviewed, and mechanisms and major afforesting techniques are summarized in this paper.
