In order to thoroughly understand the mechanism Of drought resistance in plants at DNA level, the DNA damage of two ecotypes of reeds (Phragmites communis T.) stressed by PEG 6000 was analyzed by means of fluorescence...In order to thoroughly understand the mechanism Of drought resistance in plants at DNA level, the DNA damage of two ecotypes of reeds (Phragmites communis T.) stressed by PEG 6000 was analyzed by means of fluorescence analysis of DNA unwinding (FADU). The results showed that the residual double strand DNA percentages (dsDNA%) in dune reed (DR) were significantly higher than those in swamp reed (SR) treated with either 20% or 30% PEG 6000. This meant that the DNA of DR was less damaged in comparison with SR. Similarly, DR resisted DNA damage more strongly than SR as reactive oxygen species (ROS) increased by adding ROS producers diethyldithio carbamate (DDC), H2O2 and Fe2+ of different concentrations. Meanwhile, treating PEG stressed SR with ROS scavengers such as dimethyl sulphoxide (DMSO) and ascorbic acid (Vc) resulted in the reduction of DNA damage, suggesting that ROS could cause DNA damage. In addition, the DNA repair for water-stressed reeds indicated that DR repaired DNA damage much faster and more completely. This might be the first indication that drought stress led to DNA damage in plants and that drought resistance of plants was closely related to DNA damage and repair.展开更多
Phosphorus (P) is the limiting factor for eutrophication in most freshwater ecosystems. In China, Ptransported from intensively cultivated land has been reported as an important source of P in surface waters.In this s...Phosphorus (P) is the limiting factor for eutrophication in most freshwater ecosystems. In China, Ptransported from intensively cultivated land has been reported as an important source of P in surface waters.In this study, we investigated P concentration and forms in surface and subsurface drainage from wetland ricefields in the Shaoxing plain, Zhejiang Province, China. From selected rice fields, surface drainage sampleswere collected at rice-growing, non-growing and fertilization periods, and subsurface drainage samples atdrought and rewetting (irrigation or precipitation after 5~10 d drought period in the surface soils) and wet(drainage under long-term wet soil condition) periods. Water samples were characterized for their totalreactive P (TRP), dissolved reactive P (DRP) and particulate reactive P (PRP). Concentrations of the TRPand DRP in the surface drainage ranged from 0.08 to 1.50 and 0.06 to 1.27 mg L-1, respectively. The TRPand DRP were dependent on field operation activities, and decreased in the order of fertilization period >rice-growing period > non-growing period. Phosphorus concentration of runoff receiving P fertilizer can bean environmental concern. The PRP concentration in the surface drainage, ranging from 0.01 to 0.57 mgL-1, accounted for 8%~78% of the TRP. Concentration of the TRP in the subsurface drainage was from0.026 to 0.090 mg L-1, consisting of 29%~90 % of the DRP and 10%~71% of the PRP. In the droughtand rewetting period, the PRP accounted for, on average, 63% of the TRP, much higher than in the wetperiod (23%), suggesting that there was transport of P in preferential flow during drainage events after ashort-term drought period in the surface soils. Therefore, P losses in particulate form may be importantin the subsurface drainage from rice fields when surface soils form cracks and favor rapid flow downwardthrough the soil profiles, suggesting the important role of water-dispersible colloid particles in mediating andco-transporting P in the subsurface drainage of rice fields.展开更多
This paper takes marsh in the Sanjiang Plain as an example in order to research the effect of draining on the chemical elements in marsh. The Sanjiang Ecological Test Station of Mire and Uetland serves as the resarch ...This paper takes marsh in the Sanjiang Plain as an example in order to research the effect of draining on the chemical elements in marsh. The Sanjiang Ecological Test Station of Mire and Uetland serves as the resarch base. The authors selected soil samples in the Sanjiang Plain (the top and the end of the drain, marsh soil and degeneration marsh soil), mainly analyzed contents of main ions (HCO 3 ? , Cl?, SO 4 2? and NO 3 ? ), main heavy metals (Fe, Mn, Zn and Cu), nutritive elements (N, P, K), organic matter and pH value. By testing these samples as above, the paper initially researches the effect on chemical elements content by draining by the means of the contrast of chemical elements contents between marsh soil and degenerative marsh soil and different characteristics of marsh soil elements. Results show that a lot of chemical elements had been lost because of draining.展开更多
The changes in soil organic carbon(C) mineralization as affected by anthropogenic disturbance directly determine the role of soils as C source or sink in the global C budget. The objectives of this study were to inves...The changes in soil organic carbon(C) mineralization as affected by anthropogenic disturbance directly determine the role of soils as C source or sink in the global C budget. The objectives of this study were to investigate the effects of anthropogenic disturbance(aquaculture pond, pollutant discharge and agricultural activity) on soil organic C mineralization under different water conditions in the Minjiang River estuary wetland, Southeast China. The results showed that the organic C mineralization in the wetland soils was significantly affected by human disturbance and water conditions(P < 0.001), and the interaction between human disturbance activities and water conditions was also significant(P < 0.01). The C mineralization rate and the cumulative mineralized carbon dioxide-carbon(CO_2-C)(at the 49th day) ranked from highest to lowest as follows: Phragmites australis wetland soil > aquaculture pond sediment > soil near the discharge outlet > rice paddy soil. This indicated that human disturbance inhibited the mineralization of C in soils of the Minjiang River estuary wetland, and the inhibition increased with the intensity of human disturbance. The data for cumulative mineralized CO_2-C showed a good fit(R^2 > 0.91) to the first-order kinetic model C_t = C_0(1 – exp(–kt)). The kinetic parameters C_0, k and C_0 k were significantly affected by human disturbance and water conditions. In addition, the total amount of mineralized C(in 49 d) was positively related to C_0, C_0 k and electrical conductivity of soils. These findings indicated that anthropogenic disturbance suppressed the organic C mineralization potential in subtropical coastal wetland soils, and changes of water pattern as affected by human activities in the future would have a strong influence on C cycling in the subtropical estuarine wetlands.展开更多
We investigated the chemical properties of stemflow of Picea glehnii,Abies sachalinensis and Alnus japonica as well as peat pore water chemistry,including the distance and depth profiles of pore water chemistry,in an ...We investigated the chemical properties of stemflow of Picea glehnii,Abies sachalinensis and Alnus japonica as well as peat pore water chemistry,including the distance and depth profiles of pore water chemistry,in an ombrogenous mire.The effect of stemflow on the peat pore water chemistry was clear at the stem base in the peat forest in the mire,and the peat pore water around the stem base of a tree had its own chemical properties specific to each species.P.glehnii showed the highest concentration of salts both in stemflow and peat-pore water,whereas A.japonica showed the lowest concentrations;however,the gradient of the chemical environment from the stem base to outside of the canopy is formed.The peat pore water chemistry under the canopy was mainly controlled by the chemical processes diluted by the abundant peat pore water;the stemflow movement in the high water content of the peat was more slowly because of the flat topography(〈 1o).This would be due to the fact that the chemicals in stemflow would be diluted by the abundant peat pore water.The spatial heterogeneity of chemical environment between microsites within forested peatland would be also contributed indirectly through the control of microorganism activity,and nutrient regeneration mediated the surface water and the stemflow of the dominant canopy trees.展开更多
The study was conducted in water marshes for five major marshes during the months of January, April and September of each region (Hawizeh, Hammer and the central marsh) in southern Iraq. Bacteriological analysis tha...The study was conducted in water marshes for five major marshes during the months of January, April and September of each region (Hawizeh, Hammer and the central marsh) in southern Iraq. Bacteriological analysis that some of the default pathological bacteria, including TC (Total bacterial count), FCB (faecal coliform bacteria), MPN (coliform bacteria) and FS (faecal streptococcus) can evidence to indicate of pollution. The results showed that most of the water samples examined pathological contain bacteria which indicates that the water is unfit for human consumption and the waters as basal (more hydrogen than No. 7). There was a high salinity more than the permissible limits for human consumption and even to irrigate crops, and the same case for dissolve oxygen which exceeded are other limits as drinking water.展开更多
Over the past five decades, the natural wetlands in Sanjiang Plain, Northeast China, have been extensively reclaimed for agriculture with a total loss of nearly 80% of the surface area and the undrained marshes have r...Over the past five decades, the natural wetlands in Sanjiang Plain, Northeast China, have been extensively reclaimed for agriculture with a total loss of nearly 80% of the surface area and the undrained marshes have received a large amount of exogenous nitrogen (N) input from the adjacent agricultural land because of fertilization. In the present study, the effects of nitrogen additions on seed germination and seedling biomass of Calamagrostis angustifolia in freshwater marsh were tested in a greenhouse study. Seed bank soil was exposed to different N additions (0, 5, 10, 20 and 40 g/m^2) under non-flooded water regime. Results revealed that, low level of N additions (less than 10 g/m^2) did not significantly affect the species richness and seedling density, while the seedling biomass at 5 g/m^2 of N addition was higher than other nutrient conditions. But species richness, seedling emergence and biomass decreased significantly at high level of N additions (20-40 g/m^2). The responses were species-specific. High level of N additions had negative impacts on seed germination, seedling growth and biomass of dominant species Eleocharis ovata, Calamagrostis angustifolia, duncus effusus in the seed bank. To protect and restore the wetland vegetation community in the Sanjiang plain, fertilization, irrigation and land management strategies will need to be implemented to reduce the nutrient input from the agricultural land to the wetlands.展开更多
To investigate the spatio-temporal and compositional variation of selected water quality parameters and understand the puri- fying effects of wetland in Fujin National Wetland Park (FNWP), China, the trophic level i...To investigate the spatio-temporal and compositional variation of selected water quality parameters and understand the puri- fying effects of wetland in Fujin National Wetland Park (FNWP), China, the trophic level index (TLI), paired samples t-test and correla- tion analysis were used for the statistical analysis of a set of 10 water quality parameters. The analyses were based on water samples collected from 22 stations in FNWP between 2014 and 2016. Results initially reveal that total nitrogen (TN) concentrations are above class V levels (2 mg/L), total phosphorus (TP) concentrations are below class III levels (0.2 mg/L), and that all other parameters fall within standard ranges. Highest values for TN, pH, and Chlorophyll-a were recorded in 2016, while the levels of chemical oxygen de- mand (CODMn) and biochemical oxygen demand (BODs) were lowest during this year. Similarly, TN values were highest between 2014 and 2016 while dissolved oxygen (DO) concentrations were lowest in the summer and TP concentrations were highest in the autumn. Significant variations were also found in Secchi depth (SD), TN, CODMn (P 〈 0.01), TP, and DO levels (P 〈 0.05) between the inlet and outlet of the park. High-to-low levels of TN, TP, and TDS were found in cattails, reeds, and open water (the opposite trend was seen in SD levels). Tested wetland water had a light eutrophication status in most cases and TN and TP removal rates were between 7.54%-84.36% and 37.50%-70.83%, respectively. Data also show no significant annual changes in water quality within this wetland, although obvious affects from surrounding agricultural drainage were nevertheless recorded. Results reveal a high major nutrient removal efficiency (N and P). The upper limits of these phenomena should be addressed in future research alongside a more efficient and scientific agricultural layout for the regions in and around the FNWP.展开更多
文摘In order to thoroughly understand the mechanism Of drought resistance in plants at DNA level, the DNA damage of two ecotypes of reeds (Phragmites communis T.) stressed by PEG 6000 was analyzed by means of fluorescence analysis of DNA unwinding (FADU). The results showed that the residual double strand DNA percentages (dsDNA%) in dune reed (DR) were significantly higher than those in swamp reed (SR) treated with either 20% or 30% PEG 6000. This meant that the DNA of DR was less damaged in comparison with SR. Similarly, DR resisted DNA damage more strongly than SR as reactive oxygen species (ROS) increased by adding ROS producers diethyldithio carbamate (DDC), H2O2 and Fe2+ of different concentrations. Meanwhile, treating PEG stressed SR with ROS scavengers such as dimethyl sulphoxide (DMSO) and ascorbic acid (Vc) resulted in the reduction of DNA damage, suggesting that ROS could cause DNA damage. In addition, the DNA repair for water-stressed reeds indicated that DR repaired DNA damage much faster and more completely. This might be the first indication that drought stress led to DNA damage in plants and that drought resistance of plants was closely related to DNA damage and repair.
基金Project supported by the National Natural Science Foundation of China (No.49601013).
文摘Phosphorus (P) is the limiting factor for eutrophication in most freshwater ecosystems. In China, Ptransported from intensively cultivated land has been reported as an important source of P in surface waters.In this study, we investigated P concentration and forms in surface and subsurface drainage from wetland ricefields in the Shaoxing plain, Zhejiang Province, China. From selected rice fields, surface drainage sampleswere collected at rice-growing, non-growing and fertilization periods, and subsurface drainage samples atdrought and rewetting (irrigation or precipitation after 5~10 d drought period in the surface soils) and wet(drainage under long-term wet soil condition) periods. Water samples were characterized for their totalreactive P (TRP), dissolved reactive P (DRP) and particulate reactive P (PRP). Concentrations of the TRPand DRP in the surface drainage ranged from 0.08 to 1.50 and 0.06 to 1.27 mg L-1, respectively. The TRPand DRP were dependent on field operation activities, and decreased in the order of fertilization period >rice-growing period > non-growing period. Phosphorus concentration of runoff receiving P fertilizer can bean environmental concern. The PRP concentration in the surface drainage, ranging from 0.01 to 0.57 mgL-1, accounted for 8%~78% of the TRP. Concentration of the TRP in the subsurface drainage was from0.026 to 0.090 mg L-1, consisting of 29%~90 % of the DRP and 10%~71% of the PRP. In the droughtand rewetting period, the PRP accounted for, on average, 63% of the TRP, much higher than in the wetperiod (23%), suggesting that there was transport of P in preferential flow during drainage events after ashort-term drought period in the surface soils. Therefore, P losses in particulate form may be importantin the subsurface drainage from rice fields when surface soils form cracks and favor rapid flow downwardthrough the soil profiles, suggesting the important role of water-dispersible colloid particles in mediating andco-transporting P in the subsurface drainage of rice fields.
基金One of the Key B Items of the Chinese Academy of Sciences (KZ951 -B1-201 -02 ).
文摘This paper takes marsh in the Sanjiang Plain as an example in order to research the effect of draining on the chemical elements in marsh. The Sanjiang Ecological Test Station of Mire and Uetland serves as the resarch base. The authors selected soil samples in the Sanjiang Plain (the top and the end of the drain, marsh soil and degeneration marsh soil), mainly analyzed contents of main ions (HCO 3 ? , Cl?, SO 4 2? and NO 3 ? ), main heavy metals (Fe, Mn, Zn and Cu), nutritive elements (N, P, K), organic matter and pH value. By testing these samples as above, the paper initially researches the effect on chemical elements content by draining by the means of the contrast of chemical elements contents between marsh soil and degenerative marsh soil and different characteristics of marsh soil elements. Results show that a lot of chemical elements had been lost because of draining.
基金Under the auspices of National Basic Research Program of China(No.2012CB956100)National Natural Science Foundation of China(No.41301085)the Key Foundation of Science and Technology Department of Fujian Province(No.2016R1032-1)
文摘The changes in soil organic carbon(C) mineralization as affected by anthropogenic disturbance directly determine the role of soils as C source or sink in the global C budget. The objectives of this study were to investigate the effects of anthropogenic disturbance(aquaculture pond, pollutant discharge and agricultural activity) on soil organic C mineralization under different water conditions in the Minjiang River estuary wetland, Southeast China. The results showed that the organic C mineralization in the wetland soils was significantly affected by human disturbance and water conditions(P < 0.001), and the interaction between human disturbance activities and water conditions was also significant(P < 0.01). The C mineralization rate and the cumulative mineralized carbon dioxide-carbon(CO_2-C)(at the 49th day) ranked from highest to lowest as follows: Phragmites australis wetland soil > aquaculture pond sediment > soil near the discharge outlet > rice paddy soil. This indicated that human disturbance inhibited the mineralization of C in soils of the Minjiang River estuary wetland, and the inhibition increased with the intensity of human disturbance. The data for cumulative mineralized CO_2-C showed a good fit(R^2 > 0.91) to the first-order kinetic model C_t = C_0(1 – exp(–kt)). The kinetic parameters C_0, k and C_0 k were significantly affected by human disturbance and water conditions. In addition, the total amount of mineralized C(in 49 d) was positively related to C_0, C_0 k and electrical conductivity of soils. These findings indicated that anthropogenic disturbance suppressed the organic C mineralization potential in subtropical coastal wetland soils, and changes of water pattern as affected by human activities in the future would have a strong influence on C cycling in the subtropical estuarine wetlands.
文摘We investigated the chemical properties of stemflow of Picea glehnii,Abies sachalinensis and Alnus japonica as well as peat pore water chemistry,including the distance and depth profiles of pore water chemistry,in an ombrogenous mire.The effect of stemflow on the peat pore water chemistry was clear at the stem base in the peat forest in the mire,and the peat pore water around the stem base of a tree had its own chemical properties specific to each species.P.glehnii showed the highest concentration of salts both in stemflow and peat-pore water,whereas A.japonica showed the lowest concentrations;however,the gradient of the chemical environment from the stem base to outside of the canopy is formed.The peat pore water chemistry under the canopy was mainly controlled by the chemical processes diluted by the abundant peat pore water;the stemflow movement in the high water content of the peat was more slowly because of the flat topography(〈 1o).This would be due to the fact that the chemicals in stemflow would be diluted by the abundant peat pore water.The spatial heterogeneity of chemical environment between microsites within forested peatland would be also contributed indirectly through the control of microorganism activity,and nutrient regeneration mediated the surface water and the stemflow of the dominant canopy trees.
文摘The study was conducted in water marshes for five major marshes during the months of January, April and September of each region (Hawizeh, Hammer and the central marsh) in southern Iraq. Bacteriological analysis that some of the default pathological bacteria, including TC (Total bacterial count), FCB (faecal coliform bacteria), MPN (coliform bacteria) and FS (faecal streptococcus) can evidence to indicate of pollution. The results showed that most of the water samples examined pathological contain bacteria which indicates that the water is unfit for human consumption and the waters as basal (more hydrogen than No. 7). There was a high salinity more than the permissible limits for human consumption and even to irrigate crops, and the same case for dissolve oxygen which exceeded are other limits as drinking water.
文摘Over the past five decades, the natural wetlands in Sanjiang Plain, Northeast China, have been extensively reclaimed for agriculture with a total loss of nearly 80% of the surface area and the undrained marshes have received a large amount of exogenous nitrogen (N) input from the adjacent agricultural land because of fertilization. In the present study, the effects of nitrogen additions on seed germination and seedling biomass of Calamagrostis angustifolia in freshwater marsh were tested in a greenhouse study. Seed bank soil was exposed to different N additions (0, 5, 10, 20 and 40 g/m^2) under non-flooded water regime. Results revealed that, low level of N additions (less than 10 g/m^2) did not significantly affect the species richness and seedling density, while the seedling biomass at 5 g/m^2 of N addition was higher than other nutrient conditions. But species richness, seedling emergence and biomass decreased significantly at high level of N additions (20-40 g/m^2). The responses were species-specific. High level of N additions had negative impacts on seed germination, seedling growth and biomass of dominant species Eleocharis ovata, Calamagrostis angustifolia, duncus effusus in the seed bank. To protect and restore the wetland vegetation community in the Sanjiang plain, fertilization, irrigation and land management strategies will need to be implemented to reduce the nutrient input from the agricultural land to the wetlands.
基金Under the auspices of the National Natural Science Foundation of China(No.D41271106)the National Key Research and Development Program of China(No.2016YFA0602303)
文摘To investigate the spatio-temporal and compositional variation of selected water quality parameters and understand the puri- fying effects of wetland in Fujin National Wetland Park (FNWP), China, the trophic level index (TLI), paired samples t-test and correla- tion analysis were used for the statistical analysis of a set of 10 water quality parameters. The analyses were based on water samples collected from 22 stations in FNWP between 2014 and 2016. Results initially reveal that total nitrogen (TN) concentrations are above class V levels (2 mg/L), total phosphorus (TP) concentrations are below class III levels (0.2 mg/L), and that all other parameters fall within standard ranges. Highest values for TN, pH, and Chlorophyll-a were recorded in 2016, while the levels of chemical oxygen de- mand (CODMn) and biochemical oxygen demand (BODs) were lowest during this year. Similarly, TN values were highest between 2014 and 2016 while dissolved oxygen (DO) concentrations were lowest in the summer and TP concentrations were highest in the autumn. Significant variations were also found in Secchi depth (SD), TN, CODMn (P 〈 0.01), TP, and DO levels (P 〈 0.05) between the inlet and outlet of the park. High-to-low levels of TN, TP, and TDS were found in cattails, reeds, and open water (the opposite trend was seen in SD levels). Tested wetland water had a light eutrophication status in most cases and TN and TP removal rates were between 7.54%-84.36% and 37.50%-70.83%, respectively. Data also show no significant annual changes in water quality within this wetland, although obvious affects from surrounding agricultural drainage were nevertheless recorded. Results reveal a high major nutrient removal efficiency (N and P). The upper limits of these phenomena should be addressed in future research alongside a more efficient and scientific agricultural layout for the regions in and around the FNWP.