In this study, a comparative analysis of the codon usage bias was performed in Aeropyrum pernix K1 and two other phylogenetically related Crenarchaeota microorganisms （i.e., Pyrobaculum aerophilum str. IM2 and Sulfol...In this study, a comparative analysis of the codon usage bias was performed in Aeropyrum pernix K1 and two other phylogenetically related Crenarchaeota microorganisms （i.e., Pyrobaculum aerophilum str. IM2 and Sulfolobus acidocaldarius DSM 639）. The results indicated that the synonymous codon usage in A. pernix K1 was less biased, which was highly correlated with the GC3s value. The codon usage patterns were phylogenetically conserved among these Crenarchaeota microorganisms. Comparatively, it is the species function rather than the gene function that determines their gene codon usage patterns. A. pernix K1, P. aerophilum str. IM2, and S. acidocaldarius DSM 639 live in differently extreme conditions. It is presumed that the hving environment played an important role in determining the codon usage pattern of these microorganisms. Besides, there was no strain-specific codon usage among these microorganisms. The extent of codon bias in A. pernix K1 and S. acidocaldarius DSM 639 were highly correlated with the gene expression level, but no such association was detected in P. aerophilum str. IM2 genomes.展开更多
In this paper, sediment samples were collected along the Wanquan beaches and sieved in the laboratory in order to obtain the grain size distributions and associated parameters, i.e. mean grain size, sorting coefficien...In this paper, sediment samples were collected along the Wanquan beaches and sieved in the laboratory in order to obtain the grain size distributions and associated parameters, i.e. mean grain size, sorting coefficient and skewness. Furthermore, we have calculated the longshore drift sediment transport rates and equilibrium cross-sectional areas of the entrance channel by using the method of sedimentary dynamics. The results indicate that the longshore drift sediment transport is dominated by waves with a direction from south to north, which result in rapid changes of the entrance channel. Therefore, some suggestions were proposed for improving the water quality and restoring the ecosystem of estuary. The engineering method includes increasing the sea-route of entrance channel, tidal prism and water exchanges in Shamei Lagoon.展开更多
The purpose of the investigation was to assess the opportunity to use spring waters as indicator for the load state of rural landscape ecosystems. In the periods from 2000-2003 and 2011-2012, the physical and chemical...The purpose of the investigation was to assess the opportunity to use spring waters as indicator for the load state of rural landscape ecosystems. In the periods from 2000-2003 and 2011-2012, the physical and chemical water quality of 55 freshwater springs in the German federal state of the Saarland was investigated in monthly intervals. The catchment areas of the springs represented the prevailing geological formations in the Saarland as well as the main land use types such as forest, grassland and arable land. The results show a clear correlation between the nitrate content and the share of arable land in the closer catchment area of the springs. The problem of acidification and related processes occurs mainly at the sites with acid parent rock material and accordingly substrates deficient in lime. Very low pH-values between 4 and 5 are observed and the aluminum concentration levels are consistently increased. Especially at sites with low calcium content in the geological substrate, a progressive destruction of the clay minerals can be assumed. In the extremely acidic spring waters, which showed the highest aluminum contamination during the measurement 10 years ago, the aluminum-values decreased slightly. This indicates that the clay minerals in these locations are already completely destroyed. Thus, it can be concluded that the investigation of spring waters provides information on the geo-ecological conditions in catchment areas. By means of the multi-temporal approach, changes of land use or other influences by human activities can be detected.展开更多
Polysiphonia urceolata is one type of potential commercial red seaweeds used for breeding and cultivation, because of its significant biochemical and biomedical application. However, the information of breeding and se...Polysiphonia urceolata is one type of potential commercial red seaweeds used for breeding and cultivation, because of its significant biochemical and biomedical application. However, the information of breeding and seedling incubation for cultivation is limited, especially the early development. In this study, tetrasporohyte and gametophyte of P. urceolata were taken as the study materials in Huiquan Bay, Qingdao, China. The cleaned and sterilized tetrasporophytes and gametophytes were pre-cultured in sterilized seawater, then nurtured at 18℃, 25 μmol photons m^-2 s^-1 in 12：12 h （light：dark） photoperiod. Continuous observation under microscope showed that the early development consists of bipolar division stage and seedling stage. In the division stage, tetraspores germinate into bipolar sporelings that further differentiate into a colorless rhizoidal portion and a lightly pigmented upright shoot. The lightly pigmented rhizoidal cell develops to a rhizoid and the larger pigmented cell transforms to an erect axis. In the seedling stage, several quasi-protuberances appear on the erect axis and form juvenile seedlings. The results demonstrate the culture of P. urceolata from tetraspores under laboratory conditions.展开更多
Through the development of more than 60 years, the hot spring tourism industry in China will confront with a situation of ＂innovative competition, standard development, and secondary upgrade＂. In this paper, from th...Through the development of more than 60 years, the hot spring tourism industry in China will confront with a situation of ＂innovative competition, standard development, and secondary upgrade＂. In this paper, from the perspective of product integrated marketing theory, by taking Lushan （Xingzi） hot spring as an example, the authors carry out an analysis on some problems in the development of Xingzi hot spring tourism products, propose the framework and model of the integrated development of hot spring health tourism products, and also provide some suggestions for the design of Xingzi hot spring health tourism routes.展开更多
Prediction and sensitivity models,to elucidate the response of phytoplankton biomass to environmental factors in Quanzhou Bay,Fujian,China,were developed using a back propagation(BP) network.The environmental indicato...Prediction and sensitivity models,to elucidate the response of phytoplankton biomass to environmental factors in Quanzhou Bay,Fujian,China,were developed using a back propagation(BP) network.The environmental indicators of coastal phytoplankton biomass were determined and monitoring data for the bay from 2008 was used to train,test and build a three-layer BP artificial neural network with multi-input and single-output.Ten water quality parameters were used to forecast phytoplankton biomass(measured as chlorophyll-a concentration).Correlation coefficient between biomass values predicted by the model and those observed was 0.964,whilst the average relative error of the network was-3.46% and average absolute error was 10.53%.The model thus has high level of accuracy and is suitable for analysis of the influence of aquatic environmental factors on phytoplankton biomass.A global sensitivity analysis was performed to determine the influence of different environmental indicators on phytoplankton biomass.Indicators were classified according to the sensitivity of response and its risk degree.The results indicate that the parameters most relevant to phytoplankton biomass are estuary-related and include pH,sea surface temperature,sea surface salinity,chemical oxygen demand and ammonium.展开更多
Pu Lianggong,63,is the inheritor of the local incense-making tradition in Yongchun County,Quanzhou City of southeast China’s Fujian Province.With a long history,Yongchun incense is made of hundreds of Chinese herbs u...Pu Lianggong,63,is the inheritor of the local incense-making tradition in Yongchun County,Quanzhou City of southeast China’s Fujian Province.With a long history,Yongchun incense is made of hundreds of Chinese herbs using ancient techniques.The production needs to go through over 10 procedures and requires finesse.展开更多
基金The work is supported by National Natural Science Foundation of China (No. 60121101).
文摘In this study, a comparative analysis of the codon usage bias was performed in Aeropyrum pernix K1 and two other phylogenetically related Crenarchaeota microorganisms （i.e., Pyrobaculum aerophilum str. IM2 and Sulfolobus acidocaldarius DSM 639）. The results indicated that the synonymous codon usage in A. pernix K1 was less biased, which was highly correlated with the GC3s value. The codon usage patterns were phylogenetically conserved among these Crenarchaeota microorganisms. Comparatively, it is the species function rather than the gene function that determines their gene codon usage patterns. A. pernix K1, P. aerophilum str. IM2, and S. acidocaldarius DSM 639 live in differently extreme conditions. It is presumed that the hving environment played an important role in determining the codon usage pattern of these microorganisms. Besides, there was no strain-specific codon usage among these microorganisms. The extent of codon bias in A. pernix K1 and S. acidocaldarius DSM 639 were highly correlated with the gene expression level, but no such association was detected in P. aerophilum str. IM2 genomes.
基金National Natural Science Foundation of China No.40106009+1 种基金 Major State Basic Research Development Program of China No.2002CB412401
文摘In this paper, sediment samples were collected along the Wanquan beaches and sieved in the laboratory in order to obtain the grain size distributions and associated parameters, i.e. mean grain size, sorting coefficient and skewness. Furthermore, we have calculated the longshore drift sediment transport rates and equilibrium cross-sectional areas of the entrance channel by using the method of sedimentary dynamics. The results indicate that the longshore drift sediment transport is dominated by waves with a direction from south to north, which result in rapid changes of the entrance channel. Therefore, some suggestions were proposed for improving the water quality and restoring the ecosystem of estuary. The engineering method includes increasing the sea-route of entrance channel, tidal prism and water exchanges in Shamei Lagoon.
文摘The purpose of the investigation was to assess the opportunity to use spring waters as indicator for the load state of rural landscape ecosystems. In the periods from 2000-2003 and 2011-2012, the physical and chemical water quality of 55 freshwater springs in the German federal state of the Saarland was investigated in monthly intervals. The catchment areas of the springs represented the prevailing geological formations in the Saarland as well as the main land use types such as forest, grassland and arable land. The results show a clear correlation between the nitrate content and the share of arable land in the closer catchment area of the springs. The problem of acidification and related processes occurs mainly at the sites with acid parent rock material and accordingly substrates deficient in lime. Very low pH-values between 4 and 5 are observed and the aluminum concentration levels are consistently increased. Especially at sites with low calcium content in the geological substrate, a progressive destruction of the clay minerals can be assumed. In the extremely acidic spring waters, which showed the highest aluminum contamination during the measurement 10 years ago, the aluminum-values decreased slightly. This indicates that the clay minerals in these locations are already completely destroyed. Thus, it can be concluded that the investigation of spring waters provides information on the geo-ecological conditions in catchment areas. By means of the multi-temporal approach, changes of land use or other influences by human activities can be detected.
基金Supported by National Key Technology Support Program, Development Program of China (No.2006AA09Z21)National Natural Science Foundation of China (No. 40618001 and N_CUHK438/06)Shandong Agricultural Seed Stock Breeding Project
文摘Polysiphonia urceolata is one type of potential commercial red seaweeds used for breeding and cultivation, because of its significant biochemical and biomedical application. However, the information of breeding and seedling incubation for cultivation is limited, especially the early development. In this study, tetrasporohyte and gametophyte of P. urceolata were taken as the study materials in Huiquan Bay, Qingdao, China. The cleaned and sterilized tetrasporophytes and gametophytes were pre-cultured in sterilized seawater, then nurtured at 18℃, 25 μmol photons m^-2 s^-1 in 12：12 h （light：dark） photoperiod. Continuous observation under microscope showed that the early development consists of bipolar division stage and seedling stage. In the division stage, tetraspores germinate into bipolar sporelings that further differentiate into a colorless rhizoidal portion and a lightly pigmented upright shoot. The lightly pigmented rhizoidal cell develops to a rhizoid and the larger pigmented cell transforms to an erect axis. In the seedling stage, several quasi-protuberances appear on the erect axis and form juvenile seedlings. The results demonstrate the culture of P. urceolata from tetraspores under laboratory conditions.
文摘Through the development of more than 60 years, the hot spring tourism industry in China will confront with a situation of ＂innovative competition, standard development, and secondary upgrade＂. In this paper, from the perspective of product integrated marketing theory, by taking Lushan （Xingzi） hot spring as an example, the authors carry out an analysis on some problems in the development of Xingzi hot spring tourism products, propose the framework and model of the integrated development of hot spring health tourism products, and also provide some suggestions for the design of Xingzi hot spring health tourism routes.
基金Supported by the Ocean Public Welfare Scientific Research Project,State Oceanic Administration of China(No.200705029)the National Special Fund for Basic Science and Technology of China(No.2012FY112500)the National Non-profit Institute Basic Research Fund(No.FIO2011T06)
文摘Prediction and sensitivity models,to elucidate the response of phytoplankton biomass to environmental factors in Quanzhou Bay,Fujian,China,were developed using a back propagation(BP) network.The environmental indicators of coastal phytoplankton biomass were determined and monitoring data for the bay from 2008 was used to train,test and build a three-layer BP artificial neural network with multi-input and single-output.Ten water quality parameters were used to forecast phytoplankton biomass(measured as chlorophyll-a concentration).Correlation coefficient between biomass values predicted by the model and those observed was 0.964,whilst the average relative error of the network was-3.46% and average absolute error was 10.53%.The model thus has high level of accuracy and is suitable for analysis of the influence of aquatic environmental factors on phytoplankton biomass.A global sensitivity analysis was performed to determine the influence of different environmental indicators on phytoplankton biomass.Indicators were classified according to the sensitivity of response and its risk degree.The results indicate that the parameters most relevant to phytoplankton biomass are estuary-related and include pH,sea surface temperature,sea surface salinity,chemical oxygen demand and ammonium.
文摘Pu Lianggong,63,is the inheritor of the local incense-making tradition in Yongchun County,Quanzhou City of southeast China’s Fujian Province.With a long history,Yongchun incense is made of hundreds of Chinese herbs using ancient techniques.The production needs to go through over 10 procedures and requires finesse.
