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阳陵泉运动针法联合背俞穴针罐疗法治疗寒湿痹阻型颈椎病的临床研究
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作者 李继恩 柴进华 张静 《针灸临床杂志》 2023年第12期31-36,共6页
目的:探讨阳陵泉运动针法联合背俞穴针罐疗法治疗寒湿痹阻型颈椎病的疗效。方法:选取2020年6月—2022年6月来本院治疗的颈椎病患者124例,采用区组随机分配方法分为对照组(64例)和观察组(60例),对照组给予背俞穴针罐疗法,观察组在此基础... 目的:探讨阳陵泉运动针法联合背俞穴针罐疗法治疗寒湿痹阻型颈椎病的疗效。方法:选取2020年6月—2022年6月来本院治疗的颈椎病患者124例,采用区组随机分配方法分为对照组(64例)和观察组(60例),对照组给予背俞穴针罐疗法,观察组在此基础联合阳陵泉运动针法,对比两组治疗后临床疗效、治疗前后SF-36评分、CASCS评分、NDI评分、VAS评分、中医症候积分及血清IL-6、TNF-α和IL-1β水平变化。结果:治疗后观察组疗效明显较高,差异具有统计学意义(P<0.01);两组中医主症、次症和总积分较治疗前均明显降低,差异具有统计学意义(P<0.05),且观察组降低更显著,差异具有统计学意义(P<0.01);两组CASCS评分、NDI评分和VAS评分较治疗前均明显改善,差异具有统计学意义(P<0.05),且观察组改善更显著(P<0.01);两组患者SF-36量表各条目评分显著升高,差异具有统计学意义(P<0.05),并且观察组升高更明显,差异具有统计学意义(P<0.01);两组血清IL-6、TNF-α和IL-1β水平较治疗前均明显降低,差异具有统计学意义(P<0.05),且观察组降低更显著,差异具有统计学意义(P<0.01)。结论:采用阳陵泉运动针法联合背俞穴针罐疗法治疗颈椎病具有较好的疗效。 展开更多
关键词 阳陵运动针 背俞穴 针罐疗 颈椎病
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法泉寺森林公园旅游资源结构分析和评价
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作者 蒲兴芬 《林业科技通讯》 2018年第5期100-103,共4页
从旅游资源单体类型结构、空间结构、时间结构、级别结构4方面分析法泉寺森林公园旅游资源结构,探讨了法泉寺森林公园旅游资源特征。采用专家意见评分法,对旅游资源进行总体定量评价,结果表明:法泉寺森林公园旅游资源多样性和组合性良... 从旅游资源单体类型结构、空间结构、时间结构、级别结构4方面分析法泉寺森林公园旅游资源结构,探讨了法泉寺森林公园旅游资源特征。采用专家意见评分法,对旅游资源进行总体定量评价,结果表明:法泉寺森林公园旅游资源多样性和组合性良好,整体价值为三级优良级旅游资源,资源质量高,有较大的开发潜力。 展开更多
关键词 靖远县 法泉 森林公园 旅游资源结构
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温肺缩泉法治疗小儿尿床43例 被引量:1
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作者 李昌德 《四川中医》 北大核心 2004年第2期71-71,共1页
本文报道甘草干姜汤合缩泉丸加味治疗小儿顽固性尿床 4 3例 ,有效率达 10 0 % ,临床疗效显著。
关键词 温肺缩 小儿 尿床 中医药疗
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补肾缩泉、祛瘀益智法及缩泉益智汤治疗中风后小便频数及失禁的临床研究
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作者 吕哲 杨冰 赵平丽 《中国社区医师(医学专业)》 2012年第21期229-230,共2页
2008~2012年采用补肾缩泉、祛瘀益智法及缩泉益智汤治疗中风后小便频数及失禁,疗效满意,报告如下。资料与方法将300例患者采用随机数字表法将患者随机分为两组,治疗组150例.
关键词 中风 小便频数及失禁 补肾 、祛瘀益智 益智汤
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贵州省地热流体资源可开采资源量研究
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作者 易世友 李强 +1 位作者 陈刚 吉勒克补子 《地下水》 2024年第1期85-88,共4页
综合贵州省地热研究相关成果资料,分析贵州省地热流体资源赋存条件及地热水资源分布特征,以此为基础将贵州省划分为110个地热资源量估算区,参照省内地热流体资源主要集中在断裂带周边的实际特点,采用设计布井法、泉(井)流量统计法和比... 综合贵州省地热研究相关成果资料,分析贵州省地热流体资源赋存条件及地热水资源分布特征,以此为基础将贵州省划分为110个地热资源量估算区,参照省内地热流体资源主要集中在断裂带周边的实际特点,采用设计布井法、泉(井)流量统计法和比拟法对贵州地热流体可开采资源量进行估算。结果表明:贵州地热流体资源的形成和分布,受省内地质构造特点及其在全国构造所处部位的控制,地热资源成因类型可划分为沉积盆地传导型地热储和隆起山地断裂对流型地热储,总体热储温度一般小于90℃,为低温地热资源;估算出贵州省地热流体可开采资源总量为23190.37 m^(3)/a,其中设计布井法估算的资源量为15926.22 m^(3)/a,天然温泉可开采资源量1183.34 m^(3)/a,地热井可开采资源量6080.80 m^(3)/a,总热能906933.97 Kw,年开采累计可利用热能量476.68×10^(8)MJ。所估算的地热流体可采资源量可为贵州省地热资源的勘查开发规划提供参考依据。 展开更多
关键词 地热流体 可开采资源量 设计布井 (井)流量统计 贵州省
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柳林泉域岩溶地下水资源可开采量的确定 被引量:2
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作者 张玉良 《中国煤田地质》 2002年第4期36-37,41,共3页
柳林泉域内赋存丰富的岩溶地下水资源,目前还没有大规模的开发利用。运用丰富的水文地质资料确定了泉城内的水文地质参数,概述了泉城含水系统的详细情况,用泉流量恢复法与均衡法计算并比较了泉域岩溶地下水资源量,在计算不同保证率的情... 柳林泉域内赋存丰富的岩溶地下水资源,目前还没有大规模的开发利用。运用丰富的水文地质资料确定了泉城内的水文地质参数,概述了泉城含水系统的详细情况,用泉流量恢复法与均衡法计算并比较了泉域岩溶地下水资源量,在计算不同保证率的情况下对泉域的可开采资源量进行了分配,为离柳矿区的发展、合理利用岩溶地下水资源提供了较为可靠的依据。 展开更多
关键词 岩溶 地下水资源 可开采量 流量恢复 水文地质条件
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以“黜浊治中”理论探讨针药并治前列腺癌术后尿失禁
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作者 韩增风 王超然 +3 位作者 肖贤 牛小玉 查春媛 孙彬栩 《天津中医药》 CAS 2023年第8期993-996,共4页
前列腺癌根治术后尿失禁是一种常见的术后并发症,严重影响着患者的身心健康,西医对该病的治疗虽然取得了一定疗效,但总体效果并不理想,而中医在该病的治疗优势逐渐凸显,鉴于临床上关于该病的针药并治少有探索,文章以“黜浊治中”理论探... 前列腺癌根治术后尿失禁是一种常见的术后并发症,严重影响着患者的身心健康,西医对该病的治疗虽然取得了一定疗效,但总体效果并不理想,而中医在该病的治疗优势逐渐凸显,鉴于临床上关于该病的针药并治少有探索,文章以“黜浊治中”理论探讨针药并治前列腺癌术后尿失禁,以“益气运中法”联合“缩泉止遗针法”,通过针药并治针刺导药,通调并用,靶向趋病,疗效显著。并附验案1则实践针药并用能够有效加速前列腺癌根治术后尿失禁患者尿控功能的恢复、提高患者生活质量,为临床治疗提供了思路和方法。 展开更多
关键词 前列腺癌根治术后尿失禁 黜浊治中 益气运中 止遗针
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高丽刻本《证道歌颂》考述 被引量:1
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作者 杨之峰 范猛 《图书馆学刊》 2012年第2期115-117,共3页
考订了《证道歌颂》作者释法泉的主要事迹,重点考述其现存最早的高丽刻本使用北元宣光年号的历史背景,以及刻书地中原府、青龙禅寺的历史变迁。
关键词 证道歌颂 法泉 高丽刻本 北元
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The application of the high-frequency electromagnetic sounding method in the exploration of underground thermal water 被引量:4
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作者 Cao Hui He Lanfang +1 位作者 He Zhanxiang Zhao Xiaoming 《Applied Geophysics》 SCIE CSCD 2006年第4期248-254,共7页
High-frequency electromagnetic sounding is an electromagnetic exploration method using the natural high-frequency electromagnetic field as a field source. It has higher resolution and greater depth penetration than th... High-frequency electromagnetic sounding is an electromagnetic exploration method using the natural high-frequency electromagnetic field as a field source. It has higher resolution and greater depth penetration than the direct current method and is especially fit for geothermal energy exploration and low- and mid-level groundwater detection. We introduce a successful application of high-frequency electromagnetic sounding for evaluating geothermal water resources. The high frequency electromagnetic system (MT-USA with a frequency range from 10 KHz to 1 Hz) is first applied to sample field data from China. A remote reference station is used to assure sampled data quality. We then perform 2D inversion image processing with the electrical method data. The results basically indicate the spatial distribution of underground geothermal water and provide favorable clues to finding the sources of the subsurface geothermal water in this area. 展开更多
关键词 High-frequency electromagnetic sounding geothermal resource groundwater exploration.
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Joint Network and Fountain Codes Design for RelayAssisted Multi-User System 被引量:1
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作者 HUANG Ying LEI Jing WEI Jibo 《China Communications》 SCIE CSCD 2015年第7期96-107,共12页
In relay-assisted multi-user system, relay coding is important to enhance the robustness and reliability of cooperative transmission. For better adaptability and efficiency, two joint network and fountain coding(JNFC)... In relay-assisted multi-user system, relay coding is important to enhance the robustness and reliability of cooperative transmission. For better adaptability and efficiency, two joint network and fountain coding(JNFC) schemes are proposed. When the condition of all direct channels is worse, JNFC scheme based on distributed LT(DLT) codes is used. Otherwise, JNFC scheme based on multi-dimensional LT(MD-LT) codes is suited. For both two above-mentioned schemes, the united degree distribution design method for short-length fountain codes is proposed. For the latter scheme, MD-LT codes are proposed for equal error protection(EEP) of each user. Simulation results and analysis show that the united degree distribution need less decoding overhead compared with other degree distribution for short-length fountain codes. And then, all users are protected equally in despite of asymmetric uplinks. 展开更多
关键词 relay coding network coding fountain codes LT codes Raptor codes DLT MD-LT EEP
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EFFECT OF ACUPUNCTURE PLUS MOXIBUSTION OF YONGQUAN POINT ON DYSCINESIA AND BLOOD RHEOLOGY IN STROKE PATIENTS
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作者 李佩芳 《World Journal of Acupuncture-Moxibustion》 2001年第2期13-15,共3页
Objective: To observe the therapeutic effect of acupuncture plus moxibustion of Yongquan (KI 1) in improving stroke patients’ dyskinesia and blood rheology. Methods: 78 cases of stroke patients were treated by acupun... Objective: To observe the therapeutic effect of acupuncture plus moxibustion of Yongquan (KI 1) in improving stroke patients’ dyskinesia and blood rheology. Methods: 78 cases of stroke patients were treated by acupuncture and moxibustion of Yongquan (KI 1), once daily, with 20 days being a therapeutic course. Indexes of whole blood viscosity, plasma viscosity, platelet aggregation rate, packed cell volume and fibrinogen were detected before and after treatment. Changes of the functional activity were assessed using Barthel marking method. Results: After 2 courses of acupuncture treatment, all the above mentioned indexes and functional activity were improved significantly (P<0.05~0.01). Conclusion: Acupuncture and moxibustion of Yongquan (KI 1) has an active effect in improving stroke patients’ dyskinesia and blood rheology. 展开更多
关键词 Acupuncture plus moxibustion Yongquan point Dyskinesia Blood rheology
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吉林省东部山区隧道洞室开挖涌水量估算分析
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作者 李东川 王屹林 +1 位作者 谢冬月 张立明 《吉林地质》 2014年第1期108-111,共4页
隧道洞身在中等风化岩层中,水文地质条件相对比较复杂。隧道施工时需要进行人工降水,以满足施工条件,为此估算隧道洞体涌水量在设计时显得极为重要。本文以凉水隧道工程为例,应用泉水流量汇总法、地下水径流模数法和水平廊道法对隧道涌... 隧道洞身在中等风化岩层中,水文地质条件相对比较复杂。隧道施工时需要进行人工降水,以满足施工条件,为此估算隧道洞体涌水量在设计时显得极为重要。本文以凉水隧道工程为例,应用泉水流量汇总法、地下水径流模数法和水平廊道法对隧道涌水量计算并进行对比分析,验证三种方法的合理性和有效性,为设计和施工降水提供一定的参考依据。 展开更多
关键词 隧道涌水量 水流量汇总 地下水径流模数 水平廊道
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Florida Springs--A Water-Budget Approach to Estimating Water Availability
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作者 Robert L. Knight Ronald A. Clarke 《Journal of Earth Science and Engineering》 2016年第2期59-72,共14页
Florida's artesian springs receive groundwater outflows from the Floridan Aquifer System and are concentrated north of I-4 and west to the Florida Panhandle. These springs and their resulting spring runs support a un... Florida's artesian springs receive groundwater outflows from the Floridan Aquifer System and are concentrated north of I-4 and west to the Florida Panhandle. These springs and their resulting spring runs support a unique freshwater ecology dependent on perennial flows, constant temperature and chemistry, and high light transmissivity. Numerous observations indicate that Florida's springs flows are declining as a result of the increasing extraction of groundwater for human uses. North Florida's karst environment is especially susceptible to nitrogen pollution from agricultural and urban development. An empirical springs/aquifer water budget is needed to better understand these spring stressors. Discharge data from 393 of the state's 1,000+ artesian springs are used to estimate trends in total spring discharge by decade since 1930-39. This analysis indicates that average spring flows have declined by about 32%. Large groundwater pumping centers are altering spring flows over the whole springs region. Existing groundwater pumping rates from the Floridan Aquifer in 2010 were more than 30% of average annual aquifer recharge, and allocated groundwater use in north-central Florida is nearly double current estimated uses. Based on biological research conducted in Florida springs, these flow reductions are from two to six times greater than declines known to result in significant harm to aquatic resources. 展开更多
关键词 Artesian springs Floridan Aquifer HYDROLOGY aquatic ecology aquifer recharge.
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Acupuncture treatment on 31 cases of obsolete lateral malleolus joint sprain 被引量:2
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作者 钟峰 孙婧 李晓亮 《World Journal of Acupuncture-Moxibustion》 2013年第1期59-60,共2页
Objective To observe the clinical effect of acupuncture on the obsolete lateral malleolus joint sprain. Methods Thirty-one cases of patients with obsolete lateral malleolus joint sprain were selected. Jiexi (解溪 ST... Objective To observe the clinical effect of acupuncture on the obsolete lateral malleolus joint sprain. Methods Thirty-one cases of patients with obsolete lateral malleolus joint sprain were selected. Jiexi (解溪 ST 41) were penetrated to Shenmei (申脉 BL 61), and Yanglingquan (阳陵泉 GB 34) was applied. The needles were retained for 30 min, and the treatment was performed only once, with follow-up visit after 1 month. Results All the patients were cured after treatment, and without relapse on the follow-up visit after 1 month. Conclusion The clinical effect of acupuncture on the obsolete lateral malleolus joint sprain is remarkable. 展开更多
关键词 obsolete lateral malleolus joint sprain acupuncture treatment acupoint ST 41 (Jiexi) acupoint BL 61 (Shenmai) acupoint GB 34 (Y^nglingqu^n)
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Treatment of 36 Cases of Recurrent Aphthous Ulcer with Acupoint Sticking Therapy 被引量:1
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作者 冯荣昌 洪珏 《Journal of Acupuncture and Tuina Science》 2009年第2期105-107,共3页
Objective: To observe the therapeutical effect of using the paste of Wu Zhu Yu (Fructus Evodia) for acupoint sticking combined with tonifying kidney, to treat the recurrent aphthous ulcer. Methods: Thirty-six case... Objective: To observe the therapeutical effect of using the paste of Wu Zhu Yu (Fructus Evodia) for acupoint sticking combined with tonifying kidney, to treat the recurrent aphthous ulcer. Methods: Thirty-six cases ofreccurent aphthous ulcer due to kidney dificiency were treated with Wu Zhu Yu (Fructus Evodia) paste applying to Yongquan (KI 1), and orally taking Liu Wei Di Huang Pill. Ten treatments made up one course, 2-3 d of interval between 2 courses. Then 1-year follow-up was made. Results: Twenty cases were cured, 12 cases improved, 4 cases failed. Conclusion: The method of using Wu Zhu Yu (Fructus Evodia) paste applying to acupoint Yongquan (KI 1), integrated with the manipulation of strengthening kidney, can effectively shorten the duration of disease, improve the symptoms, and control recurrency, which is good for this disease. 展开更多
关键词 Stomatitis Aphthous Acupoint Sticking Therapy Points Yongquan (KI 1) EVODIA
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Analysis of points selection pattern in acupuncture treatment of sleep apnea syndrome based on data mining 被引量:3
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作者 曹淼 张林 +2 位作者 贲定严 何清湖 吴江昀 《Journal of Acupuncture and Tuina Science》 CSCD 2018年第1期53-58,共6页
To explore the points selection pattern of acupuncture for sleep apnea syndromes by data mining technique. Methods: Clinical literature about acupuncture therapy for sleep apnea syndromes was derived from China Natio... To explore the points selection pattern of acupuncture for sleep apnea syndromes by data mining technique. Methods: Clinical literature about acupuncture therapy for sleep apnea syndromes was derived from China National Knowledge Infrastructure (CNKI), Wanfang Academic Journal Full-text Database (Wanfang), Chongqing VIP Database (CQVIP), PubMed and Science Direct between the time that databases were created and March 25th,2017. Relevant excel database was established and descriptive studies and association rules were analyzed. Results: The most frequently used point was Lianquan (CV 23) and the most frequently used meridian was the Stomach Meridian. The analysis of association rules showed that the clinical choice of acupuncture points was highly correlated, among which the combination of the highest degree of confidence and the highest degree of support was Shenmen (HT 7) and Sishencong (EX-HN 1); Lieque (LU 7), lianquan (CV 23) and Zhaohai (KI 6). Conclusion: Acupuncture treatment of sleep apnea syndromes has specific selection rules of points, providing certain references for clinical and scientific research. 展开更多
关键词 Acupuncture-moxibustion Therapy Acupuncture Therapy Point Lianquan (CV 23) Points Head Neck SleepApnea Syndromes Data Mining Point Selection
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Clinical observation of Zhen'ai needling method in Nei Jing(Classic of Internal Medicine)for children with allergic rhinitis accompanied by adenoid hypertrophy 被引量:2
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作者 Zhang Cui-hong Liu Zhan-wen +4 位作者 Hong JueLiu Jie Xie Chen Wu Ling-xiang Yang Yan-ting Ma Xiao-peng 《Journal of Acupuncture and Tuina Science》 CSCD 2020年第4期308-314,共7页
Objective:To observe the clinical efficacy of Zhen'ai needling method in Nei Jing(Classic of Internal Medicine)for children with allergic rhinitis(AR)accompanied by adenoid hypertrophy(AH).Methods:A total of 74 ch... Objective:To observe the clinical efficacy of Zhen'ai needling method in Nei Jing(Classic of Internal Medicine)for children with allergic rhinitis(AR)accompanied by adenoid hypertrophy(AH).Methods:A total of 74 children who met the screening criteria were divided into a Zhen'ai group and a control group by the random number table method,with 37 cases in each group.The control group was treated with acupuncture at Zusanli(ST 36),Hegu(LI 4),Yingxiang(LI 20),Juliao(ST 3),Yintang(GV 29),Shangxing(GV 23)and Baihui(GV 20).The Zhen'ai group added points of Zhen'ai needling method{Shanglianquan[Extra,located at 1 cun above Lianquan(CV 23)],Tianrong(SI 17)and Lieque(LU 7)}in addition to the points in the control group.The needles were retained for 30 min.The treatment was performed twice a week.The total nasal symptom score(TNSS),sino-nasal outcome test-20(SNOT-20)and symptom scale for AH(SSAH)were assessed before and after 10 treatments.The clinical efficacy of the two groups was compared after treatment.Results:During the treatment,5 cases dropped out in the control group and 2 cases in the Zhen'ai group.After treatment,the total effective rate of the Zhen'ai group was 94.3%,versus 93.8%in the control group.There was no significant difference between the two groups(P>0.05).The markedly effective rate of the Zhen'ai group was 42.9%,versus 12.5%in the control group,and the difference was statistically significant(P<0.05).After treatment,there were significant intra-group differences in the scores of TNSS,SNOT-20 and SSAH in both groups(all P<0.05);the scores of SNOT-20 and SSAH in the Zhen'ai group were lower than those in the control group,and the differences between the groups were statistically significant(both P<0.05).Conclusion:Both conventional acupuncture and conventional acupuncture plus Zhen'ai needling method can improve clinical symptoms of children with AR accompanied by AH;and conventional acupuncture plus Zhen'ai needling method has a better effect than conventional acupuncture in improving AH symptoms. 展开更多
关键词 Acupuncture-moxibustion Therapy Acupuncture Therapy Point Shanglianquan(Extra) Point Tianrong(SI 17) Point Lieque(LU 7) Rhinitis Allergic Adenoid Hypertrophy
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Effect of acupuncture at complementary acupionts on mast cells and histamine/bradykinin released by mast cells in the regions of acupionts 被引量:3
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作者 屈敬阳 安娜 +4 位作者 刘丁丁 陈龙 许倩 杨志新 杨燕萍 《Journal of Acupuncture and Tuina Science》 CSCD 2018年第1期8-13,共6页
Objective: To observe the changes in numbers and degranulation status of local mast cells (MCs) of the complementary acupionts, as well as the concentrations of serum histamine and bradykinin after acupuncture at ... Objective: To observe the changes in numbers and degranulation status of local mast cells (MCs) of the complementary acupionts, as well as the concentrations of serum histamine and bradykinin after acupuncture at rat's complementary acupionts of Yanglingquan (GB 34) and Yinlingquan (SP 101, for exploring the mechanisms of the synergistic effect of complementary acupionts. Methods: Using random number table method, 40 Wistar rats were randomly divided into a blank control group (group K), an acupuncture at Yanglingquan (GB 34) group (group A), an acupuncture at Yinlingquan (SP 9) group (group B), an acupuncture at Yanglingquan (GB 34) and Yinlingquan (SP 9) group (group AB), and an acupuncture at points near Yanglingquan (GB 34) and Yinlingquan (SP 9) group (group CD), 8 rats in each group. Group A received acupuncture at Yanglingquan (GB 34); group B received acupuncture at Yinlingquan (SP 9); group AB received acupuncture at Yanglingquan (GB 34) and Yinlingquan (SP 9); group CD received acupuncture at the control points [points 3 mm away from Yanglingquan (GB 34) and Yinlingquan (SP 9) respectively]. Acupuncture was performed at bilateral points in each group. Rats in group K were fixed using the same method as rats in the other 4 groups without acupuncture stimulation. Needles of 0.35 mm in diameter and 40 mm in length were used for acupuncture. Needle handle was connected to the G6805-]~[ mode electroacupuncture (EA) device after needling qi was obtained, with sparse-dense wave, frequency of 2 Hz/100 Hz and current of i mA to keep the needle handles slightly tremulous while the rats kept quiet. Rats were continuously stimulated by EA for 20 rain each time. Experimental interventions were conducted on the 1st, 3rd, 5th and 7th days when the experiment started, for 4 times in total. Specimens from rats in each group were collected 2 h after the 7th day. The levels of histamine and bradykinin in serum were detected by enzyme-linked immunosorbent assay (ELISA). Local tissues of the points were used to prepare cryosections. The changes of MCs were observed after toluidine blue staining. Results: Compared with group K, the numbers and degranulation rates of MCs in group A, group B, group AB and group CD were significantly increased (all P〈0.05). The number and degranulation rate of MCs in group AB were significantly higher than those in group A, group B and group CD (all P〈0.05). The order of histamine levels from high to low was: group AB 〉 group B 〉 group A 〉 group CD 〉 group K, and the differences were statistically significant (all P〈0.05); the levels of bradykinin in group AB, group A and group B were significantly higher than those in group K and group CD (all P〈O.05). There was no significant difference in the bradykinin level between group A and group B, nor between group CD and group K (all P〉0.05). Conclusion: The number and degranulation rate of MCs of the complementary acupionts are significantly increased after acupuncture at complementary acupionts of Yanglingquan (GB 34) and Yinlingquan (SP 9) of rats, and the serum levels of histamine and bradykinin are increased, which may be one of the mechanisms of the synergism produced by the combination of complementary acuDionts. 展开更多
关键词 Complementary Acupionts Point Combination Acupuncture Therapy Electroacupuncture Point Yinlingquan(SP 9) Point Yanglingquan (GB 34) Mast Cell HISTAMINE
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Effect of electroacupuncture on JAK2/STAT3 pathway in synovial tissues of rats with rheumatoid arthritis 被引量:5
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作者 Fan Wei-min Zhao Chun-jiang 《Journal of Acupuncture and Tuina Science》 CSCD 2019年第4期223-230,共8页
Objective: To observe the effect of electroacupuncture (EA) on Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway in knee joint synovial tissues of rats with rheumatoid arthritis (R... Objective: To observe the effect of electroacupuncture (EA) on Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway in knee joint synovial tissues of rats with rheumatoid arthritis (RA) and to explore the action mechanism of EA on RA. Methods: Twelve of the 48 SPF male Sprague-Dawley (SD) rats were assigned to a normal group by the random number table method. The remaining 36 rats were subjected to RA model preparation by intradermal injection of the Freund's complete adjuvant into the right hind foot pad of each rat under sterile conditions. After the model was successfully prepared, rats were then divided into a model group, a drug group and an EA group according to a random number table method (n=12). Rats in the drug group were treated with 2 mL aqueous solution of tripterygium glycosides [8.1 mg/(kg?bw)];rats in the EA group were treated with EA at bilateral Yanglingquan (GB 34) and Zusanli (ST 36), for 30 min each time;rats in the normal group and the model group were placed in a special rat fixation tank for 30 min each time, and received the same dose of normal saline as those in the drug group. Rats in all groups received intervention once a day for 4 weeks. Diameter of rat ankle joint and rat arthritis index were measured before and after the intervention. At the end of the experiment, the expressions of phospho-JAK2 and phospho-STAT3 were determined by immunohistochemistry. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect JAK2 and STAT3 mRNAs expressions. Results: After the model was produced, the arthritis index >2 was considered successful in model preparation. Compared with the model group, the ankle joint diameters and arthritis indexes of rats in the drug group and the EA group were significantly lower (all P<0.01);immunohistochemical staining cells with phospho-JAK2 and phospho-STAT3 were significantly decreased (all P<0.01);the expression levels of JAK2 and STAT3 mRNAs were decreased with statistical differences (all P<0.01). There were no significant differences between the EA group and the drug group (all P>0.05). Conclusion: EA can alleviate the inflammatory response of RA rats, improve their pathological conditions, reduce the expressions of phospho-JAK2 and phospho-STAT3 in the synovial tissue of knee joint, and decrease the expressions of JAK2 and STAT3 mRNAs. The therapeutic effect of EA is comparable to that of the tripterygium glycosides. The mechanism of EA treatment may be related to the inactivation of the JAK2/STAT3 pathway. 展开更多
关键词 Acupuncture Therapy ELECTROACUPUNCTURE Point Yanglingquan (GB 34) Point Zusanli (ST 36) Arthritis Rheumatoid Janus Kinase 2/Signal Transducer and Activator of Transcription 3 (JAK2/STAT 3) Pathway RATS
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Effect of electroacupuncture at Lower He-Sea points including Yanglingquan(GB 34)on nuclear factor-κB and interleukin-1βin guinea pigs with acute cholecystitis 被引量:1
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作者 Ni Wei Zhang Jian 《Journal of Acupuncture and Tuina Science》 CSCD 2020年第4期247-255,共9页
Objective:To observe the effect of electroacupuncture(EA)at Lower He-Sea points on the expression levels of interleukin-1β(IL-1β)in the serum and gallbladder tissues,and nuclear factor-κB(NF-κB)in gallbladder tiss... Objective:To observe the effect of electroacupuncture(EA)at Lower He-Sea points on the expression levels of interleukin-1β(IL-1β)in the serum and gallbladder tissues,and nuclear factor-κB(NF-κB)in gallbladder tissues of the guinea pigs with acute cholecystitis(AC),and to explore whether Yanglingquan(GB 34),the Lower He-Sea point pertaining to Dan Fu(gallbladder),is relatively specific for the Dan Fu(gallbladder)disorders.Methods:Eighty-two healthy guinea pigs were randomly divided into 6 groups according to the random number table method,a blank group,a model group,a Yanglingquan(GB 34)group,a Zusanli(ST 36)group,a Shangjuxu(ST 37)group,and a Xiajuxu(ST 39)group,with 12 guinea pigs in the blank group while 14 in the other groups,respectively,half males and half females in each group.Except for the blank group,guinea pigs in the other groups were injected with E.coli into the gallbladder to establish AC models.Guinea pigs in the blank group were fed routinely without special treatment;those in the model group were daily tied up for 30 min without EA treatment;those in the 4 groups receiving EA treatment were acupunctured at the corresponding Lower He-Sea points after daily binding and stimulated with the SDZ-V EA instrument.After successful modeling and treatment for 5 d,blood was collected from the abdominal aorta of the guinea pigs,and the gallbladder tissues in each group were isolated for hematoxylin-eosin(HE)staining to observe the morphological changes.Enzyme-linked immunosorbent assay(ELISA)was used to detect the serum IL-1βlevel,and immunohistochemistry(IHC)was used to detect the expression levels of NF-κB and IL-1βin gallbladder.Results:On the 3rd day after modeling,the guinea pigs in the five groups with modeling were mentally depressed with decreased appetite,significantly reduced activities,slouch,lassitude,slack and matted fur,and loose stools;two guinea pigs were selected from each group(one male and one female,not included in the final statistics)to isolate the gallbladder after sacrifice;macroscopic observation showed that the gallbladder wall was differently thickened;the bile color was dark green and opaque with particles suspended or accumulated;light microscope observation showed that the submucosal blood vessels of the gallbladder were congested,along with mucosal edema,ulceration,necrosis,shedding,and a large number of inflammatory cells infiltrating in the lamina propria,indicating that the AC model was successfully prepared.Compared with the model group,the gallbladder tissue injuries of the four groups receiving EA treatment were all differently repaired,the serum IL-1βlevels were reduced(P<0.01 or P<0.05),and the IL-1βlevels in the gallbladder tissues were reduced(P<0.01 or P<0.05).Compared with the model group,the NF-κB expression level in the Yanglingquan(GB 34)group was significantly reduced(P<0.01),but was not statistical different in the Zusanli(ST 36)group,Shangjuxu(ST 37)group and Xiajuxu(ST 39)group(all P>0.05).Compared with the Yanglingquan(GB 34)group,the gallbladder tissues of the Zusanli(ST 36)group,Shangjuxu(ST 37)group and Xiajuxu(ST 39)group were more severely damaged,and the expression levels of serum IL-1β,the NF-κB and IL-1βin the gallbladder tissues were increased(P<0.01 or P<0.05).Intervention effect of Yanglingquan(GB 34)on AC guinea pigs was superior to that of Zusanli(ST 36),Shangjuxu(ST 37)and Xiajuxu(ST 39).Conclusion:EA at the Lower He-Sea points of the stomach,large intestine,small intestine and gallbladder can produce curative effects on AC guinea pigs and reduced the inflammatory symptoms.Intervention effect of Yanglingquan(GB 34)on AC guinea pigs is superior to that of Zusanli(ST 36),Shangjuxu(ST 37)and Xiajuxu(ST 39).The mechanism of EA at Yanglingquan(GB 34)in treating AC may be regulating IL-1βand NF-κB to control the inflammatory response and improve the gallbladder tissue damage. 展开更多
关键词 Acupuncture Therapy ELECTROACUPUNCTURE Point Yanglingquan(GB 34) Point Lower He-Sea Interleukin-1 beta NF-kappa B Cholecystitis Acute Guinea Pigs
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