This study investigated the potential application of a zirconium oxide （ZrO2） ceramic foam culturing system to the production of glial cell line-derived neurotrophic factor （GDNF）. Three sets of ZrO2 ceramic foams...This study investigated the potential application of a zirconium oxide （ZrO2） ceramic foam culturing system to the production of glial cell line-derived neurotrophic factor （GDNF）. Three sets of ZrO2 ceramic foams with different pore densities of 10, 20, and 30 pores per linear inch （PPI） were prepared to support a 3D culturing system. After primary astrocytes were cultured in these systems, production yields of GDNF were evaluated. The biomaterial bio- compatibility, cell proliferation and activation of cellular signaling pathways in GDNF synthesis and secretion in the culturing systems were also assessed and compared with a conventional culturing system. In this study, we found that the ZrO2 ceramic foam culturing system was biocompatible, using which the GDNF yields were elevated and sustained by stimulated cell proliferation and activation of signaling pathways in astrocytes cultured in the system. In conclusion, the ZrO2 ceramic foam is promising for the development of a GDNF mass production device for Parkinson＇s disease treatment.展开更多
基金Project supported by the National Natural Science Foundation of China(Nos.81171262 and 81371473)
文摘This study investigated the potential application of a zirconium oxide （ZrO2） ceramic foam culturing system to the production of glial cell line-derived neurotrophic factor （GDNF）. Three sets of ZrO2 ceramic foams with different pore densities of 10, 20, and 30 pores per linear inch （PPI） were prepared to support a 3D culturing system. After primary astrocytes were cultured in these systems, production yields of GDNF were evaluated. The biomaterial bio- compatibility, cell proliferation and activation of cellular signaling pathways in GDNF synthesis and secretion in the culturing systems were also assessed and compared with a conventional culturing system. In this study, we found that the ZrO2 ceramic foam culturing system was biocompatible, using which the GDNF yields were elevated and sustained by stimulated cell proliferation and activation of signaling pathways in astrocytes cultured in the system. In conclusion, the ZrO2 ceramic foam is promising for the development of a GDNF mass production device for Parkinson＇s disease treatment.
