玉米蛋白发泡粉生产新工艺的研究

在用碱水解玉米麸质粉生产玉米蛋白发泡粉的基础上 ,就玉米蛋白发泡粉得率低 ,纯度低 ,色泽深等问题进行了研究。采用玉米麸质粉入胶体磨细化再用碱水解 ,利用超滤膜分离技术 ,超高温瞬时灭菌 ,真空喷雾干燥等生产新工艺 ,产品蛋白质纯度高达 93% ,玉米蛋白发泡粉得率提高到 83.4 % 。

玉未蛋白发泡粉复合法制备工艺

采用酶法辅助碱法的工艺生产玉米蛋白发泡粉,实验结果表明,当反应温度45℃,反应时间为1h,酶底物比为0.001U.g-1,pH为7.5时,水解液发泡性达到460%,泡沫稳定性为100%。

用玉米砂制食用菌母种技术

用玉米砂制母种,既节约了成本,又可省去繁琐的工艺,特别适合小规模的食用菌栽培户应用,现把我多年的制作经验介绍如下: 首先,把玉米粉碎。过筛去细粉,取玉米砂备用。再在5000ml水里加入研碎的进口复合肥5g、白糖7.5g,溶化后调PH7.5～8,取上清液浸泡玉米砂5～7小时,捞出玉米砂沥干水后倒入干净布上,放锅中蒸熟透,冷至常温,搓成散粒,分装入试管至1/3高,用力甩紧,并用接种耙轻压料面,取一小棉球用清水浸湿。

Effect Analysis on the Soaking Technics of Corn Wet Milling in the Laboratory

[Objective] The research aimed to find the optimal condition of corn starch production in the laboratory and analyze the soaking effect.[Method] The orthogonal test was used to determine the suitable technological condition.By the light microscope and the scanning electron microscope,the soaking effect was analyzed.[Result] The suitable soaking condition was：soaking time 48 h,soaking temperature 55 ℃ and SO2 concentration 0.2%.The microscopic analysis result was that the protein matrix was sufficiently decomposed in the suitable soaking condition.The soaking effect had the positive correlation with the decomposed degree of protein matrix.[Conclusion] The research provided the basis for the soaking technics research of corn starch in the laboratory.

Existence and Characteristics of Tonoplast-bound Protein Kinase in the Tip Cell of Maize Root

For understanding the function of tonoplast protein in plant cell signal pathway, we have identified an integral protein kinase activity from the highly purified tonoplast isolated from maize ( Zea mays L.) root by a new nonradioactive method in which a color labeled peptide was used as substrate. The protein kinase was Ca 2+ _dependent and CaM and phosphatidylserine_independent, like the calmodulin_like domain protein kinase (CDPK) in many plants. The optimal pH value and Ca 2+ concentration were 6.5 and 10 μmol/L, respectively. According to the optimal pH value and the effect of detergent, it could be inferred that the active site of this protein kinase is oriented toward the cytoplasm. Zn 2+ had no obvious effect on its activity, indicating that this protein kinase has no zinc_finger domain that exists in some mammalian protein kinases. At the same time, when tonoplast proteins were prephosphorylated in the presence of Ca 2+ and ATP, both the ATP_hydrolysis and the proton_transport activity of vacuolar H +_ATPase were stimulated. This stimulation could be reversed by an alkaline_phosphatase. These results indicate that a Ca 2+ _dependent protein kinase was located in the tonoplast, and a Ca 2+ _dependent phosphorylation, probably caused by this kinase, activated the vacuolar H +_ATPase activity. These results are helpful for further research on the function of CDPK in the course of signal transduction in plants.

利用米糠制取植酸钙效益好

植酸钙是一种医药化工原料,在工业上普遍用作肌醇原料,而我国肌醇产量90％外销,供不应求,其植酸钙价格连续上涨。江苏省淮阴市标准计量情报所下属玉米淀粉厂从浸泡玉米的废水中提取植酸钙,投产三个月来,大见成效,每天可提取植酸钙1.5吨,一年可创产值225万元,除去投资设备等一切支出外,年获纯利150万元。 此外,利用米糠、麸皮作原料提取植酸钙投资小、见效快、成本低,发展潜力巨大。加工后的米糠、麸皮颜色味道均未改变,作饲料营养价值更高,是一种很理想的致富门路。为了满足市场需求,该所下属厂准备将此技术进行转让。