Paulownia tomentosa, P. fargesii, P. lamprorhylla, P. albiphloea, P. australis, P. fortunei, P. elongata, P. elongata f.alba and P. albiphloea var c henggtuensis were classified into three groups: P. fortunei group (...Paulownia tomentosa, P. fargesii, P. lamprorhylla, P. albiphloea, P. australis, P. fortunei, P. elongata, P. elongata f.alba and P. albiphloea var c henggtuensis were classified into three groups: P. fortunei group (P. fortunei a nd P. elongata f. alba); P. australis group (P. australis and P. albiphloea var chenggtuensis) and P. tomentasa group (P. tomentasa, P. fargesii, P. albiprhlaca , P. lamproprhylia and P. elongata ) accordance to the results of the single and two-dimensional SDS-PAGE of protein in the Paulownia tree leaves. The result co uld lay a foundation for classifying the Genus Paulownia plants.展开更多
[Objective] The research aimed to find the optimal condition of corn starch production in the laboratory and analyze the soaking effect.[Method] The orthogonal test was used to determine the suitable technological con...[Objective] The research aimed to find the optimal condition of corn starch production in the laboratory and analyze the soaking effect.[Method] The orthogonal test was used to determine the suitable technological condition.By the light microscope and the scanning electron microscope,the soaking effect was analyzed.[Result] The suitable soaking condition was：soaking time 48 h,soaking temperature 55 ℃ and SO2 concentration 0.2%.The microscopic analysis result was that the protein matrix was sufficiently decomposed in the suitable soaking condition.The soaking effect had the positive correlation with the decomposed degree of protein matrix.[Conclusion] The research provided the basis for the soaking technics research of corn starch in the laboratory.展开更多
Physarum polycephalum L., a naturally synchronized myxomycophyta, was demonstrated to contain a cyclin B1-like protein by Western blot and immunoelectron microscopy. The content and subcellular location of the protein...Physarum polycephalum L., a naturally synchronized myxomycophyta, was demonstrated to contain a cyclin B1-like protein by Western blot and immunoelectron microscopy. The content and subcellular location of the protein varied during the cell cycle. The cyclin B1-like protein was first detected in the plasmodia of S phase while it did not appear in the nuclei until late G2 phase. The content of the protein in both the plasmodia and nuclei rose gradually onwards, peaked at metaphase and disappeared abruptly at ana-telophase. The protein was found to be distributed in both the cytoplasm and nuclei in late G2 phase and metaphase. In nuclei, the protein was mainly located in the chromosomal and nucleolar areas. The results suggest that the cyclin B1-like protein of P. polycephalum begins to be synthesized at S phase, enters the nuclei at late G2 phase, accumulates in both cytoplasm and nuclei onwards and breaks down at ana-telophase. The results also suggest that the cyclin B1-like protein acts as a cytoplasmic-nuclear protein during certain phases of the cell cycle.展开更多
The classification, characters and maturation methods of VPEs were sum- marized, and its regulation function to vacuolar was also analyzed. Furthermore, effects of the enzyme in vacuolar-mediated plant defense mechani...The classification, characters and maturation methods of VPEs were sum- marized, and its regulation function to vacuolar was also analyzed. Furthermore, effects of the enzyme in vacuolar-mediated plant defense mechanism were discussed to point out that VPEs were divided into 3 subfamilies via autocatalytic mature including seed-type VPE, vegetative-type VPE and new-type VPE. Especially, seed- type VPE mediated the process of storage protein, while vegetative-type VPE and new-type VPE regulated and controlled programmed death of plant cells.展开更多
The most striking morphological feature of eukaryotic cells is the presence of various membrane-enclosed compartments. These compartments, including organelles and transient transport intermediates, are not static. Ra...The most striking morphological feature of eukaryotic cells is the presence of various membrane-enclosed compartments. These compartments, including organelles and transient transport intermediates, are not static. Rather, dynamic exchange of proteins and membrane is needed to maintain cellular homeostasis. One of the most dramatic examples of membrane mobilization is seen during the process ofmacroautophagy. Macroautophagy is the primary cellular pathway for degradation of long-lived proteins and organelles. In response to environmental cues, such as starvation or other types of stress, the cell produces a unique membrane structure, the phagophore. The phagophore sequesters cytoplasm as it forms a double-membrane cytosolic vesicle, an autophagosome. Upon completion, the autophagosome fuses with a lysosome or a vacuole in yeast, which delivers hydrolases that break down the inner autophagosome membrane along with its cargo, and the resulting macromolecules are released back into the cytosol for reuse. Autophagy is therefore a recycling process, allowing cells to survive periods of nutrient limitation; however, it has a wider physiological role, participating in development and aging, and also in protection against pathogen invasion, cancer and certain neurodegenerative diseases. In many cases, the role ofautophagy is identified through studies of an autophagy-related protein, Atg6/Beclin 1. This protein is part of a lipid kinase complex, and recent studies suggest that it plays a central role in coordinating the cytoprotective function ofautophagy and in opposing the cellular death process of apoptosis. Here, we summarize our current knowledge ofAtg6/Beclin 1 in different model organisms and its unique function in the cell.展开更多
基金National Nature Science Foundation of China and Nature Science Foundation of Henan Province.
文摘Paulownia tomentosa, P. fargesii, P. lamprorhylla, P. albiphloea, P. australis, P. fortunei, P. elongata, P. elongata f.alba and P. albiphloea var c henggtuensis were classified into three groups: P. fortunei group (P. fortunei a nd P. elongata f. alba); P. australis group (P. australis and P. albiphloea var chenggtuensis) and P. tomentasa group (P. tomentasa, P. fargesii, P. albiprhlaca , P. lamproprhylia and P. elongata ) accordance to the results of the single and two-dimensional SDS-PAGE of protein in the Paulownia tree leaves. The result co uld lay a foundation for classifying the Genus Paulownia plants.
文摘[Objective] The research aimed to find the optimal condition of corn starch production in the laboratory and analyze the soaking effect.[Method] The orthogonal test was used to determine the suitable technological condition.By the light microscope and the scanning electron microscope,the soaking effect was analyzed.[Result] The suitable soaking condition was：soaking time 48 h,soaking temperature 55 ℃ and SO2 concentration 0.2%.The microscopic analysis result was that the protein matrix was sufficiently decomposed in the suitable soaking condition.The soaking effect had the positive correlation with the decomposed degree of protein matrix.[Conclusion] The research provided the basis for the soaking technics research of corn starch in the laboratory.
文摘Physarum polycephalum L., a naturally synchronized myxomycophyta, was demonstrated to contain a cyclin B1-like protein by Western blot and immunoelectron microscopy. The content and subcellular location of the protein varied during the cell cycle. The cyclin B1-like protein was first detected in the plasmodia of S phase while it did not appear in the nuclei until late G2 phase. The content of the protein in both the plasmodia and nuclei rose gradually onwards, peaked at metaphase and disappeared abruptly at ana-telophase. The protein was found to be distributed in both the cytoplasm and nuclei in late G2 phase and metaphase. In nuclei, the protein was mainly located in the chromosomal and nucleolar areas. The results suggest that the cyclin B1-like protein of P. polycephalum begins to be synthesized at S phase, enters the nuclei at late G2 phase, accumulates in both cytoplasm and nuclei onwards and breaks down at ana-telophase. The results also suggest that the cyclin B1-like protein acts as a cytoplasmic-nuclear protein during certain phases of the cell cycle.
基金Supported by National Natural Science Foundation of China (Grant No.30840002,30970223)Scientific Research Fund of Heilongjiang for Returned Chinese Scholars(Grant No. LC08C03)+3 种基金Special Fund for Basic Research in Higher Education Institutions of China (Grant No.DL09DA02)Scientific Research Starting Fund for Introduced Talents in Northeast Forestry University (Grant No. 015-602042)National Science Fund for Post-doctoral Scientists of China (Grant No. 200902365)Preferred Science-Technology Fund for Returned Chinese Scholars in Helongjiang (Grant No. 2009-HLJLixinLi)~~
文摘The classification, characters and maturation methods of VPEs were sum- marized, and its regulation function to vacuolar was also analyzed. Furthermore, effects of the enzyme in vacuolar-mediated plant defense mechanism were discussed to point out that VPEs were divided into 3 subfamilies via autocatalytic mature including seed-type VPE, vegetative-type VPE and new-type VPE. Especially, seed- type VPE mediated the process of storage protein, while vegetative-type VPE and new-type VPE regulated and controlled programmed death of plant cells.
文摘The most striking morphological feature of eukaryotic cells is the presence of various membrane-enclosed compartments. These compartments, including organelles and transient transport intermediates, are not static. Rather, dynamic exchange of proteins and membrane is needed to maintain cellular homeostasis. One of the most dramatic examples of membrane mobilization is seen during the process ofmacroautophagy. Macroautophagy is the primary cellular pathway for degradation of long-lived proteins and organelles. In response to environmental cues, such as starvation or other types of stress, the cell produces a unique membrane structure, the phagophore. The phagophore sequesters cytoplasm as it forms a double-membrane cytosolic vesicle, an autophagosome. Upon completion, the autophagosome fuses with a lysosome or a vacuole in yeast, which delivers hydrolases that break down the inner autophagosome membrane along with its cargo, and the resulting macromolecules are released back into the cytosol for reuse. Autophagy is therefore a recycling process, allowing cells to survive periods of nutrient limitation; however, it has a wider physiological role, participating in development and aging, and also in protection against pathogen invasion, cancer and certain neurodegenerative diseases. In many cases, the role ofautophagy is identified through studies of an autophagy-related protein, Atg6/Beclin 1. This protein is part of a lipid kinase complex, and recent studies suggest that it plays a central role in coordinating the cytoprotective function ofautophagy and in opposing the cellular death process of apoptosis. Here, we summarize our current knowledge ofAtg6/Beclin 1 in different model organisms and its unique function in the cell.
