The injection of a high pressure gas into a stagnant liquid pool is the characteristic phenomenon during the expansion phase of a hypothetical core disruptive accident in liquid metal cooled fast reactors. In order to...The injection of a high pressure gas into a stagnant liquid pool is the characteristic phenomenon during the expansion phase of a hypothetical core disruptive accident in liquid metal cooled fast reactors. In order to investigate lots of mechanisms involved in this phase of the accident＇s evolution, an experimental campaign called S GI was performed in 1994 in Forschungszentrum Karlsruhe, now KIT. This campaign consists of nine experiments which have been dedicated to assess the effects of different pressure injection, the nozzle＇s size and the presence of inner confinement in the formation of the rising bubble. Three of these experiments, which were focused on the pressure effects, have now been simulated with SIMMER III code and with FLUENT 6.3, a commercial CFD code. Both codes, despite their different features, have showed a good agreement with the experimental results. In particular, time trend evolutions of pressures and bubble volumes have been well reproduced by simulation. Furthermore, both codes agree on the shape of the bubble, even though they have evidenced same discrepancies with the experimental shape.展开更多
Objective To explore whether the amount of lipocalin-2 in the biofluid could reflect the onset of sepsis-induced acute lung injury(ALI) in mice. Methods Lipopolysaccharide(LPS, 10 mg/kg) injection or cecal ligation an...Objective To explore whether the amount of lipocalin-2 in the biofluid could reflect the onset of sepsis-induced acute lung injury(ALI) in mice. Methods Lipopolysaccharide(LPS, 10 mg/kg) injection or cecal ligation and puncture(CLP) was performed to induce severe sepsis and ALI in C57 BL/6 male mice randomly divided into 5 groups(n=10 in each group): group A(intraperitoneal LPS injection), group B(intravenous LPS injection via tail vein), group C(CLP with 25% of the cecum ligated), group D(CLP with 75% of the cecum ligated), and the control group(6 sham-operation controls plus 4 saline controls). All the mice received volume resuscitation. Measurements of pulmonary morphological and functional alterations were used to identify the presence of experimental ALI. The expressions of lipocalin-2 and interleukin(IL)-6 in serum, bronchoalveolar lavage fluid(BALF), and lung tissue were quantified at both protein and mRNA levels. The overall abilities of lipocalin-2 and IL-6 tests to diagnose sepsis-induced ALI were evaluated by generating receiver operator characteristic curves(ROC) and computing area under curve(AUC). Results In both group B and group D, most of the "main features" of experimental ALI were reproduced in mice, while group A and group C showed septic syndrome without definite evidence for the presence of ALI. Compared with septic mice without ALI(group A+group C), lipocalin-2 protein expression in septic mice with ALI(group B+group D) was significantly up-regulated in BALF(P<0.01) and in serum(P<0.01), and mRNA expression boosted in lung tissues(all P<0.05). Lipocalin-2 tests performed better than IL-6 tests in recognizing sepsis-induced ALI cases, evidenced by the larger AUC of the former(BALF tests, 0.8800 versus 0.6625; serum tests, 0.8500 versus 0.7000). Using a dual cutoff system to diagnose sepsis-induced ALI, BALF lipocalin-2 test exhibited the highest positive likelihood ratio(13.000) and the lowest negative likelihood ratio(0.077) among the tests of lipocalin-2 and IL-6 in blood and BALF. A statistically significant correlation was found between lipocalin-2 concentration in BALF and that in serum(Spearman r=0.8803,P<0.0001). Conclusions Lipocalin-2 expression is significantly up-regulated in septic ALI mice compared with those without ALI. Lipocalin-2 tests with a dual cutoff system could be an effective tool in distinguishing experimental ALI cases.展开更多
The bubble formation process at submerged orifices with different geometry is investigated in the preparation of aluminum foams by gas injection method.The bubble profile on a horizontal plate is calculated by quasi-s...The bubble formation process at submerged orifices with different geometry is investigated in the preparation of aluminum foams by gas injection method.The bubble profile on a horizontal plate is calculated by quasi-static analysis through Laplace equation.The bubble formation process is then distinguished into three stages:nucleation stage,growth stage and detachment stage in wetting and less wetting conditions based on the force balance analysis.In addition,the bubble size at high Reynolds number is obtained by considering the contribution of buoyancy,pressure force,inertial force,drag force and surface tension based on the three stages of bubble formation.The bubble size is confirmed to be sensitive to the equivalent contact angle,which consists of two terms including the contact angle and the wedge angle.Therefore,the wedge angle is introduced in the design of gas outlet orifices for the purpose of decreasing bubble size generated.The experimental study is conducted at three different types of stainless steel orifices under constant gas flow rates(0.05–2 L/min).It is clarified that the orifice geometry and the orifice size are both responsible for the cell size of aluminum foams.The experimental results for three different types of orifices show a consistent trend with the theoretical predictions at various gas flow rates.In the design of orifices to generate small bubbles in the melt,the wedge angle that coordinates with the contact angle is thus suggested.展开更多
文摘The injection of a high pressure gas into a stagnant liquid pool is the characteristic phenomenon during the expansion phase of a hypothetical core disruptive accident in liquid metal cooled fast reactors. In order to investigate lots of mechanisms involved in this phase of the accident＇s evolution, an experimental campaign called S GI was performed in 1994 in Forschungszentrum Karlsruhe, now KIT. This campaign consists of nine experiments which have been dedicated to assess the effects of different pressure injection, the nozzle＇s size and the presence of inner confinement in the formation of the rising bubble. Three of these experiments, which were focused on the pressure effects, have now been simulated with SIMMER III code and with FLUENT 6.3, a commercial CFD code. Both codes, despite their different features, have showed a good agreement with the experimental results. In particular, time trend evolutions of pressures and bubble volumes have been well reproduced by simulation. Furthermore, both codes agree on the shape of the bubble, even though they have evidenced same discrepancies with the experimental shape.
基金Supported in part by Jie-shou Li Academician Gut Barrier Research Fund(2012001)
文摘Objective To explore whether the amount of lipocalin-2 in the biofluid could reflect the onset of sepsis-induced acute lung injury(ALI) in mice. Methods Lipopolysaccharide(LPS, 10 mg/kg) injection or cecal ligation and puncture(CLP) was performed to induce severe sepsis and ALI in C57 BL/6 male mice randomly divided into 5 groups(n=10 in each group): group A(intraperitoneal LPS injection), group B(intravenous LPS injection via tail vein), group C(CLP with 25% of the cecum ligated), group D(CLP with 75% of the cecum ligated), and the control group(6 sham-operation controls plus 4 saline controls). All the mice received volume resuscitation. Measurements of pulmonary morphological and functional alterations were used to identify the presence of experimental ALI. The expressions of lipocalin-2 and interleukin(IL)-6 in serum, bronchoalveolar lavage fluid(BALF), and lung tissue were quantified at both protein and mRNA levels. The overall abilities of lipocalin-2 and IL-6 tests to diagnose sepsis-induced ALI were evaluated by generating receiver operator characteristic curves(ROC) and computing area under curve(AUC). Results In both group B and group D, most of the "main features" of experimental ALI were reproduced in mice, while group A and group C showed septic syndrome without definite evidence for the presence of ALI. Compared with septic mice without ALI(group A+group C), lipocalin-2 protein expression in septic mice with ALI(group B+group D) was significantly up-regulated in BALF(P<0.01) and in serum(P<0.01), and mRNA expression boosted in lung tissues(all P<0.05). Lipocalin-2 tests performed better than IL-6 tests in recognizing sepsis-induced ALI cases, evidenced by the larger AUC of the former(BALF tests, 0.8800 versus 0.6625; serum tests, 0.8500 versus 0.7000). Using a dual cutoff system to diagnose sepsis-induced ALI, BALF lipocalin-2 test exhibited the highest positive likelihood ratio(13.000) and the lowest negative likelihood ratio(0.077) among the tests of lipocalin-2 and IL-6 in blood and BALF. A statistically significant correlation was found between lipocalin-2 concentration in BALF and that in serum(Spearman r=0.8803,P<0.0001). Conclusions Lipocalin-2 expression is significantly up-regulated in septic ALI mice compared with those without ALI. Lipocalin-2 tests with a dual cutoff system could be an effective tool in distinguishing experimental ALI cases.
基金supported by the International Cooperation Project of Ministry of Science and Technology of China(Grant No.2013DFR50330)
文摘The bubble formation process at submerged orifices with different geometry is investigated in the preparation of aluminum foams by gas injection method.The bubble profile on a horizontal plate is calculated by quasi-static analysis through Laplace equation.The bubble formation process is then distinguished into three stages:nucleation stage,growth stage and detachment stage in wetting and less wetting conditions based on the force balance analysis.In addition,the bubble size at high Reynolds number is obtained by considering the contribution of buoyancy,pressure force,inertial force,drag force and surface tension based on the three stages of bubble formation.The bubble size is confirmed to be sensitive to the equivalent contact angle,which consists of two terms including the contact angle and the wedge angle.Therefore,the wedge angle is introduced in the design of gas outlet orifices for the purpose of decreasing bubble size generated.The experimental study is conducted at three different types of stainless steel orifices under constant gas flow rates(0.05–2 L/min).It is clarified that the orifice geometry and the orifice size are both responsible for the cell size of aluminum foams.The experimental results for three different types of orifices show a consistent trend with the theoretical predictions at various gas flow rates.In the design of orifices to generate small bubbles in the melt,the wedge angle that coordinates with the contact angle is thus suggested.
