We evaluated the seasonal variation in plankton community composition in an artificial lake. We conducted microscopic analysis and denaturing gradient gel electrophoresis (DGGE) of PCR-amplified partial 16S rRNAand 18...We evaluated the seasonal variation in plankton community composition in an artificial lake. We conducted microscopic analysis and denaturing gradient gel electrophoresis (DGGE) of PCR-amplified partial 16S rRNAand 18S rRNAgenes to characterize the plankton community. The clustering of unweighted pair group method with arithmetic mean (UPGMA) was then used to investigate the similarity of these plankton communities. DGGE fingerprinting revealed that samples collected at the different sites within a season shared high similarity and were generally grouped together. In contrast, we did not observe any seasonal variation based on microscopic analysis. Redundancy analysis (RDA) of the plankton operational taxonomic units (OTUs) in relation to environmental factors revealed that transparency was negatively correlated with the first axis (R=-0.931), and temperature and total phosphorus (TP) were positively correlated with the first axis (R=0.736 and R=0.660, respectively). In conclusion, plankton communities in the artificial lake exhibited significant seasonal variation. Transparency, phosphorus and temperature appear to be the major factors driving the differences in plankton composition.展开更多
The diversity,community structure and seasonal variation in demersal nekton off the Changjiang(Yangtze)River estuary was evaluated using monthly trawl survey data,collected between December 2008 and November 2009.A to...The diversity,community structure and seasonal variation in demersal nekton off the Changjiang(Yangtze)River estuary was evaluated using monthly trawl survey data,collected between December 2008 and November 2009.A total of 95 species(56 teleosts,11 cephalopods,and 28 decapod crustaceans)from 69 genera,49 families and 15 orders were collected.These species could be classifi ed into six groups on the basis of temporal distribution patterns.The resident crab Ovalipes punctatus dominated the community,both in number and biomass.A clear seasonal succession was observed in the species composition.Cluster analysis revealed three primary seasonal groups corresponding to the samples collected in winter-spring,late spring-summer and late summer-autumn.The highest biomass and lowest diversity were observed in summer,while the lowest biomass and highest diversity in winter.The abundance-biomass comparison curves and community composition suggested that the investigated community was moderately disturbed.The results suggest that reduction in fi shing pressure and in the degree of seasonal hypoxia are essential for sustainable resource management off the Changjiang River estuary.展开更多
基金Supported by the National Basic Research Program of China (973 Program) (No. 2007CB109205)the National Natural Science Foundation of China (No. 30970553)
文摘We evaluated the seasonal variation in plankton community composition in an artificial lake. We conducted microscopic analysis and denaturing gradient gel electrophoresis (DGGE) of PCR-amplified partial 16S rRNAand 18S rRNAgenes to characterize the plankton community. The clustering of unweighted pair group method with arithmetic mean (UPGMA) was then used to investigate the similarity of these plankton communities. DGGE fingerprinting revealed that samples collected at the different sites within a season shared high similarity and were generally grouped together. In contrast, we did not observe any seasonal variation based on microscopic analysis. Redundancy analysis (RDA) of the plankton operational taxonomic units (OTUs) in relation to environmental factors revealed that transparency was negatively correlated with the first axis (R=-0.931), and temperature and total phosphorus (TP) were positively correlated with the first axis (R=0.736 and R=0.660, respectively). In conclusion, plankton communities in the artificial lake exhibited significant seasonal variation. Transparency, phosphorus and temperature appear to be the major factors driving the differences in plankton composition.
基金Supported by the National Key Technology R&D Program of China(No.2007BAD43B01)the National Special Research Fund for Non-Profit Sector(Agriculture)(No.201303047)the Special Fund of Chinese Central Government for Basic Scientific Research Operations in Commonweal Research Institutes(No.2008T04)
文摘The diversity,community structure and seasonal variation in demersal nekton off the Changjiang(Yangtze)River estuary was evaluated using monthly trawl survey data,collected between December 2008 and November 2009.A total of 95 species(56 teleosts,11 cephalopods,and 28 decapod crustaceans)from 69 genera,49 families and 15 orders were collected.These species could be classifi ed into six groups on the basis of temporal distribution patterns.The resident crab Ovalipes punctatus dominated the community,both in number and biomass.A clear seasonal succession was observed in the species composition.Cluster analysis revealed three primary seasonal groups corresponding to the samples collected in winter-spring,late spring-summer and late summer-autumn.The highest biomass and lowest diversity were observed in summer,while the lowest biomass and highest diversity in winter.The abundance-biomass comparison curves and community composition suggested that the investigated community was moderately disturbed.The results suggest that reduction in fi shing pressure and in the degree of seasonal hypoxia are essential for sustainable resource management off the Changjiang River estuary.