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饲粮非纤维性碳水化合物/中性洗涤纤维对黔北麻羊瘤胃发酵参数、血浆生化指标和养分消化率的影响 被引量:11
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作者 赵鹏 吴文旋 +7 位作者 吴佳海 朱伦琴 莘海亮 袁鑫 杨春红 杨康 孙伦 韩娥 《动物营养学报》 CAS CSCD 北大核心 2017年第7期2565-2574,共10页
本试验旨在研究不同饲粮非纤维性碳水化合物(NFC)/中性洗涤纤维(NDF)对黔北麻羊瘤胃发酵参数、血浆生化指标和养分消化率的影响。采用3×3拉丁方试验设计,选取6只健康成年黔北麻羊为试验动物,将其分为3组,每组2个重复,每个重复1只... 本试验旨在研究不同饲粮非纤维性碳水化合物(NFC)/中性洗涤纤维(NDF)对黔北麻羊瘤胃发酵参数、血浆生化指标和养分消化率的影响。采用3×3拉丁方试验设计,选取6只健康成年黔北麻羊为试验动物,将其分为3组,每组2个重复,每个重复1只羊。试验Ⅰ组、试验Ⅱ组、试验Ⅲ组饲粮NFC/NDF分别为2.14∶1.00、1.05∶1.00、0.40∶1.00(实测值)。试验分3期,每期15 d,包括10 d预试期和5 d正试期。检测指标包括:饲粮养分消化率、瘤胃发酵参数(pH、缓冲力、氨态氮和挥发性脂肪酸浓度、纤维素酶活性)、血浆生化指标(脂多糖、白蛋白、尿素氮、葡萄糖含量及过氧化氢酶、谷草转氨酶、超氧化物歧化酶、谷胱甘肽过氧化物酶活性)。结果显示:1)3个试验组干物质采食量差异不显著(P>0.05),试验Ⅲ组各养分消化率均显著低于试验Ⅰ组、试验Ⅱ组(P<0.05)。2)试验Ⅰ组瘤胃液pH显著低于试验Ⅱ组、试验Ⅲ组(P<0.05);试验Ⅲ组瘤胃液缓冲力显著高于试验Ⅰ组(P<0.05),纤维二糖酶活性、乙酸浓度显著低于试验Ⅰ组(P<0.05),总挥发性脂肪酸、丁酸浓度显著低于另外2组(P<0.05);试验Ⅰ组、试验Ⅱ组、试验Ⅲ组瘤胃液乙酸/丙酸依次升高、丙酸浓度依次下降,各组间差异显著(P<0.05);3组间瘤胃液羧甲基纤维素酶、木聚糖酶、微晶纤维素酶活性差异不显著(P>0.05)。3)试验Ⅰ组、试验Ⅱ组、试验Ⅲ组血浆脂多糖含量依次显著下降(P<0.05),其余血浆生化指标差异不显著(P>0.05)。综合来看,过高NFC/NDF对黔北麻羊瘤胃发酵参数和血浆生化指标有不良影响,本试验条件下,饲粮NFC/NDF以1.05∶1.00为宜。 展开更多
关键词 纤维碳水化合物/中洗涤纤维 瘤胃发酵参数 血浆生化指标 养分消化率 黔北麻羊
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饲粮非纤维性碳水化合物/中性洗涤纤维对肉犊牛生长性能和营养物质消化代谢的影响 被引量:21
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作者 李岚捷 成述儒 +4 位作者 刁其玉 付彤 王安思 李明 屠焰 《动物营养学报》 CAS CSCD 北大核心 2017年第6期2143-2152,共10页
本试验旨在研究饲粮非纤维性碳水化合物/中性洗涤纤维(NFC/NDF)对断奶肉犊牛生长性能、血清生化指标和营养物质消化代谢的影响。选取2~3月龄健康、平均体重为(94.38±0.25)kg的断奶肉犊牛60头,随机分为4组,每组15头。分别饲喂粗蛋... 本试验旨在研究饲粮非纤维性碳水化合物/中性洗涤纤维(NFC/NDF)对断奶肉犊牛生长性能、血清生化指标和营养物质消化代谢的影响。选取2~3月龄健康、平均体重为(94.38±0.25)kg的断奶肉犊牛60头,随机分为4组,每组15头。分别饲喂粗蛋白质水平相近,NFC/NDF分别为1.35(A组)、1.23(B组)、0.94(C组)和0.80(D组)的4种全混合日粮。试验期105 d,其中预试期15 d,正试期90 d。每日测定采食量,每隔15 d测量犊牛的体重;于15、30、45、60、75和90 d颈静脉采血测定血清葡萄糖(GLU)、生长激素(GH)、胰岛素样生长因子-Ⅰ(IGF-Ⅰ)、瘦素(LEP)、胰岛素(INS)、胰高血糖素(PG)和甘油三酯(TG)的浓度;分别在30和90 d时以全收粪尿法进行消化代谢试验。结果表明:1)高NFC/NDF饲粮提高了犊牛的平均日增重,A组显著高于其他3组(P<0.05);2)A组血清LEP浓度显著高于C组和D组(P<0.05),D组血清IGF-Ⅰ浓度显著高于其他各组(P<0.05);3)90 d时,干物质、中性洗涤纤维和酸性洗涤纤维的表观消化率及总能消化率、总能代谢率和消化能代谢率随饲粮NFC/NDF的降低而降低,A组均显著高于D组(P<0.05),D组甲烷能显著高于其他组(P<0.05),A组尿能、尿氮和消化氮显著高于B组和C组(P<0.05)。综上所述,NFC/NDF为1.35的饲粮可以满足3~6月龄肉犊牛对营养物质的需求,采食该饲粮不但可以使肉犊牛保持较高的平均日增重(1.14 kg/d),而且此饲粮易消化利用,采食后相关血清生化指标均在正常范围内,并未影响犊牛健康。 展开更多
关键词 肉犊牛 纤维碳水化合物/中洗涤纤维 生长 血清生化指标 消化代谢
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不同饲养模式下饲粮非纤维性碳水化合物/中性洗涤纤维对生长期杜寒杂交母羊生长性能、营养物质表观消化率和甲烷产量的影响 被引量:12
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作者 周艳 许贵善 +3 位作者 董利锋 邓凯东 马涛 刁其玉 《动物营养学报》 CAS CSCD 北大核心 2018年第4期1367-1376,共10页
本试验旨在借助开路式呼吸测热系统研究饲粮非纤维性碳水化合物(NFC)/中性洗涤纤维(NDF)对生长期杜寒杂交母羊生长性能、营养物质表观消化率及甲烷产量的影响。采用单因素试验设计,选取体况良好、体重为(27.8±0.5)kg的母羊30只,根... 本试验旨在借助开路式呼吸测热系统研究饲粮非纤维性碳水化合物(NFC)/中性洗涤纤维(NDF)对生长期杜寒杂交母羊生长性能、营养物质表观消化率及甲烷产量的影响。采用单因素试验设计,选取体况良好、体重为(27.8±0.5)kg的母羊30只,根据体重一致原则,分配到3组,各组饲粮NFC/NDF分别为0.78(精粗比为35∶65)、1.03(精粗比为50∶50)、2.17(精粗比为65∶35),每组10只羊。试验期为25 d,包括3 d调整期、7 d预试期和15 d正试期。结果表明:在NFC/NDF=0.78组自由采食和其他组限饲条件下,3组试验羊的初始体重和结束体重以及平均日增重无显著性差异(P>0.05)。当饲粮NFC/NDF由0.78增加至2.17时,干物质采食量显著降低(P<0.05),干物质、有机物和粗蛋白质表观消化率显著增加(P<0.05),NDF和酸性洗涤纤维(ADF)表观消化率无显著性差异(P>0.05)。当饲粮NFC/NDF由0.78增加至2.17时,甲烷能、单位干物质采食量的甲烷产量、单位总能摄入量的甲烷能产量显著降低(P<0.05)。结果提示,在平均日增重一致的前提下,高NFC/NDF饲粮料重比较低,且甲烷转化效率较低;对生长期杜寒杂交母羊来说,限饲条件下饲喂NFC/NDF为2.17的饲粮在提高生产效益的同时又兼顾甲烷减排,效果相对最佳。 展开更多
关键词 甲烷 纤维碳水化合物/中洗涤纤维 生长 消化代谢 呼吸测热
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不同非纤维性碳水化合物与中性洗涤纤维比例饲粮中添加酿酒酵母对绵羊体外瘤胃发酵的影响 被引量:7
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作者 郑玮才 郝小燕 +4 位作者 张春香 项斌伟 张文佳 温灏宇 张建新 《动物营养学报》 CAS CSCD 北大核心 2019年第11期5354-5366,共13页
本试验旨在研究不同非纤维性碳水化合物与中性洗涤纤维比例(NFC/NDF)饲粮中添加酿酒酵母对绵羊体外瘤胃发酵的影响。试验采用3×5双因子试验设计,在3种NFC/NDF[0.79(A1)、0.89(A2)和1.10(A3)]饲粮中添加5种水平[0(B1)、2×10^11... 本试验旨在研究不同非纤维性碳水化合物与中性洗涤纤维比例(NFC/NDF)饲粮中添加酿酒酵母对绵羊体外瘤胃发酵的影响。试验采用3×5双因子试验设计,在3种NFC/NDF[0.79(A1)、0.89(A2)和1.10(A3)]饲粮中添加5种水平[0(B1)、2×10^11(B2)、4×10^11(B3)、6×10^11(B4)和8×10^11 CFU/kg(B5)]的酿酒酵母,制成15种发酵底物,测定体外发酵48 h后的产气参数、甲烷(CH 4)产量、瘤胃发酵指标和养分降解率。结果表明:1)饲粮NFC/NDF和酿酒酵母添加量对24、48 h累积产气量和产气参数均产生了显著影响(P<0.05),二者的交互作用对24、48 h累积产气量的影响显著(P<0.05),对产气参数的影响不显著(P>0.05)。2)饲粮NFC/NDF和酿酒酵母添加量及二者的交互作用对CH 4产量的影响均不显著(P>0.05);随着饲粮NFC/NDF的增加,体外发酵液中总挥发性脂肪酸(TVFA)和丙酸浓度逐渐增加,乙酸、丁酸浓度和乙丙比逐渐降低。酿酒酵母添加量为6×10^11 CFU/kg时,体外发酵液中TVFA和丙酸浓度最高,乙酸浓度和乙丙比最低,该组乙酸浓度显著低于B1、B2、B5组(P<0.05),乙丙比显著低于B1、B3、B5组(P<0.05)。饲粮NFC/NDF与酿酒酵母添加量的交互作用对CH 4产量以及体外发酵液中TVFA、乙酸、丙酸、丁酸浓度和乙丙比均无显著影响(P>0.05)。3)随着饲粮NFC/NDF的增加,体外发酵液pH和氨态氮(NH 3-N)浓度逐渐降低,均表现为A1组显著高于A2、A3组(P<0.05);饲粮NFC/NDF为0.89时体外发酵液微生物蛋白(MCP)浓度最高,显著高于饲粮NFC/NDF为0.79和1.10时(P<0.05)。酿酒酵母添加量对体外发酵液NH 3-N和MCP浓度的影响显著(P<0.05),酿酒酵母添加量为6×10^11 CFU/kg时NH 3-N浓度最低,MCP浓度最高。饲粮NFC/NDF与酿酒酵母添加量的交互作用对体外发酵液pH无显著影响(P>0.05),但对体外发酵液NH 3-N和MCP浓度有显著影响(P<0.05)。4)饲粮NFC/NDF对发酵底物干物质降解率(DMD)、中性洗涤纤维降解率(NDFD)和酸性洗涤纤维降解率(ADFD)均有显著影响(P<0.05),而酿酒酵母添加量对除NDFD之外的其他指标均有显著影响(P<0.05),二者的交互作用对DMD有显著影响(P<0.05)。综上所述,饲粮NFC/NDF和酿酒酵母添加量以及二者的交互作用均会影响绵羊瘤胃体外发酵,饲粮中添加适量酿酒酵母可以促进体外瘤胃发酵,稳定发酵液pH,提高饲料的利用率。整体来看,饲粮NFC/NDF为0.89或1.10、酿酒酵母添加量为6×10^11 CFU/kg时对绵羊体外瘤胃发酵的促进效果最好。 展开更多
关键词 纤维碳水化合物与中洗涤纤维比例 酿酒酵母 绵羊 体外瘤胃发酵
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饲粮非纤维性碳水化合物/中性洗涤纤维对藏羊瘤胃发酵参数及菌群结构的影响 被引量:7
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作者 李蒋伟 周力 +5 位作者 马博研 高占红 姚有莉 王志有 侯生珍 桂林生 《四川农业大学学报》 CSCD 北大核心 2022年第2期253-259,275,共8页
【目的】旨在研究不同非纤维性碳水化合物/中性洗涤纤维(NFC/NDF)对藏羊瘤胃发酵参数及微生物区系多样性的影响。【方法】选取2月龄健康、平均体重为(21.27±0.38)kg的断奶母羔15只,随机分为3组,每组5只。分别饲喂粗蛋白水平相似,NF... 【目的】旨在研究不同非纤维性碳水化合物/中性洗涤纤维(NFC/NDF)对藏羊瘤胃发酵参数及微生物区系多样性的影响。【方法】选取2月龄健康、平均体重为(21.27±0.38)kg的断奶母羔15只,随机分为3组,每组5只。分别饲喂粗蛋白水平相似,NFC/NDF水平为2.20(Ⅰ组)、1.39(Ⅱ组)和0.92(Ⅲ组)的全混合饲粮。试验期持续119 d,其中预试期7 d,正试期112 d。试验结束后采集瘤胃液测定细菌多样性及各类细菌相对丰度。【结果】①随着NFC/NDF水平的降低,瘤胃液pH显著增加,瘤胃液中氨态氮浓度、丙酸和异丁酸占总挥酸含量的百分比以及总挥发酸含量逐渐降低,乙酸百分比及乙酸丙酸比逐渐增加(P<0.05)。②3组共产生347种操作分类单元(OTU),其中共有OTU为269种,占总OTU的77.52%。③Ⅰ组的Shannon指数、ACE指数和Chao1指数均显著低于Ⅲ组(P<0.05)。④门水平上,拟杆菌门(Bacteroidetes)相对丰度Ⅱ组显著高于其余两组(P<0.05),厚壁菌门随NFC/NDF降低而降低(P<0.05);⑤科水平上,毛螺菌科(Lachnospiraceae)Ⅰ组显著高于Ⅱ组(P<0.05),瘤胃球菌科(Ruminococcaceae)Ⅰ组和Ⅱ组显著高于Ⅲ组(P<0.05);韦荣氏菌科(Veillonellaceae)Ⅱ组显著高于Ⅰ组和Ⅲ组(P<0.05)。⑥属水平上,丁酸弧菌属(Butyrivibrio)Ⅱ组显著高于Ⅰ组和Ⅲ组(P<0.05);瘤胃球菌属(Ruminococcus)相对丰度有降低趋势,且Ⅰ组和Ⅱ组显著高于Ⅲ组(P<0.05)。⑦主坐标分析瘤胃细菌第一主成分和第二主成分贡献率分别为63.93%和57.63%,各组间瘤胃液细菌群落结构差异明显,Ⅰ组与Ⅱ组明显分隔。【结论】饲喂不同NFC/NDF水平饲粮,改变了瘤胃发酵模式,显著影响了育肥藏羊瘤胃中微生物的种类和丰富度,并影响门、科和属水平上细菌的组成。 展开更多
关键词 纤维碳水化合物/中洗涤纤维 藏羊 瘤胃发酵 16S rDNA 瘤胃微生物
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5个热带豆科牧草洗涤纤维的生育期动态及其施肥响应 被引量:6
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作者 潘伟彬 陈志彤 +1 位作者 陈恩 黄毅斌 《草地学报》 CAS CSCD 2008年第6期652-658,共7页
为了解不同牧草品种在不同肥力条件下洗涤性纤维的生育期动态变化,采用多因素完全随机区组盆栽试验,测定了热研2号柱花草(S1)(Stylosanthes guianensis(Aubl.)Sw.cv.CIAT184)、圆叶决明CPI34721(S2)(Chamaecrista rotundifo... 为了解不同牧草品种在不同肥力条件下洗涤性纤维的生育期动态变化,采用多因素完全随机区组盆栽试验,测定了热研2号柱花草(S1)(Stylosanthes guianensis(Aubl.)Sw.cv.CIAT184)、圆叶决明CPI34721(S2)(Chamaecrista rotundifolia(Pers.)Greene cv.CPI34721)、圆叶决明CPI86134(S3)(C.rotundifoliacv.CPI86134)、圆叶决明ATF3248(S5)(C.rotundifoliacv.ATF3248)、羽叶决明ATF2217(S5)(C.nictitans(L.)Moench cv.ATF2217)在不施肥(F0)、常量施肥(F1)、加倍施肥(F2)情况下,以及生长期内7月25日(C1)、8月29日(C2)、10月8日(C3)、11月14日(C4)分四次进行刈割的牧草洗涤性纤维含量。结果表明:不同牧草品种的洗涤性纤维含量具有较大差异,中性洗涤纤维(NDF)以S4、S1最高(53.4%、52.7%),S5、S3最低(47.9%、46.9%);酸性洗涤纤维(ADF)和酸性洗涤木质素(ADL)均为S5(23.0%、9.1%)极显著地低于其他4个品种(28.0%-29.0%、11.1%-12.3%)(P〈0.01)。施肥试验表明,F2极显著地降低了牧草的ADF含量(P〈0.01),对NDF、ADL影响不显著。施肥极显著地降低了牧草在C3刈割期的洗涤纤维含量(P〈0.01),F1和F2的NDF、ADF、ADL分别比F0下降6.6、5.4,9.7、6.9,2.6、1.6个百分点,且F2的ADF、ADL含量显著低于F1(P〈0.05)。刈割试验表明,5个品种的洗涤纤维均随生长时间持续上升,尤其是ADF平均值从C3期的7.9%急剧上升到C4期的24.6%。采用粗饲料相对值(RFV)评价方法表明,S5的平均RFV值最高(143.7),S4、S1最低(118.2、120.9);S5在C3期的RFV值为116,比S4、S1提高16个分值;施肥可以提高RFV值,且与施肥量成正比。该研究结果可为亚热带地区牧草品种选择、最适刈割期和适宜施肥量的确定提供技术借鉴。 展开更多
关键词 热带豆科牧草 洗涤性纤维 生育期动态 施肥
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亚急性瘤胃酸中毒对奶山羊血浆异常代谢产物及生化指标的影响 被引量:6
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作者 孙燕勇 胡红莲 +2 位作者 高民 徐明 宋利文 《动物营养学报》 CAS CSCD 北大核心 2017年第3期1046-1055,共10页
本试验通过递增饲粮非纤维性碳水化合物与中性洗涤纤维比(NFC/NDF)方式诱导奶山羊发生亚急性瘤胃酸中毒(SARA),旨在探讨SARA诱导过程中奶山羊血浆组胺(HIS)、脂多糖(LPS)含量及生化指标的变化。选取4只体况良好、体重接近的泌乳期萨能... 本试验通过递增饲粮非纤维性碳水化合物与中性洗涤纤维比(NFC/NDF)方式诱导奶山羊发生亚急性瘤胃酸中毒(SARA),旨在探讨SARA诱导过程中奶山羊血浆组胺(HIS)、脂多糖(LPS)含量及生化指标的变化。选取4只体况良好、体重接近的泌乳期萨能奶山羊,依次饲喂NFC/NDF为1.40、1.79、2.31、3.23的4种饲粮,每种饲粮饲喂15 d(为1组),前12 d为适应期,后3 d为采样期。酶联免疫吸附测定(ELISA)法检测血浆中HIS、LPS的含量,生化分析仪测定血浆生化指标。结果表明:1)当饲粮NFC/NDF由1.40递增至3.23时,成功诱导奶山羊处于SARA状态。2)在诱导SARA发生过程中,随着饲粮NFC/NDF增加,血浆HIS和LPS含量增加,NFC/NDF为3.23时显著高于NFC/NDF为1.40时(P<0.05)。3)随着饲粮NFC/NDF增加,血浆免疫球蛋白M(Ig M)、免疫球蛋白A(Ig A)、免疫球蛋白G(Ig G)含量无显著变化(P>0.05);血浆肌酐(CREA)、D-乳酸(LD)含量及肌酸激酶(CK)、谷草转氨酶(AST)、二胺氧化酶(DAO)活性无显著变化(P>0.05);血浆γ-谷氨酰转移酶(γ-GT)活性呈升高趋势,NFC/NDF为3.23时显著高于其他各组(P<0.05);血浆尿素氮(UN)含量降低,NFC/NDF为1.40时显著高于其他各组(P<0.05);血浆碱性磷酸酶(ALP)活性升高,NFC/NDF为3.23时极显著高于其他3组(P<0.01);血浆游离脂肪酸(FFA)含量先升高后降低,且在NFC/NDF为2.31时极显著高于其他各组(P<0.01);血浆β-羟丁酸(β-HB)含量降低,NFC/NDF为1.40时呈极显著高于其他各组(P<0.01)。结果提示,随着饲粮NFC/NDF递增,血浆中HIS和LPS含量显著增加,引发了机体炎症反应,加重了瘤胃上皮的损伤和SARA病情,激发奶山羊免疫活化状态;诱导SARA发生过程中,血浆生化指标有不同程度的变化,提示SARA期间奶山羊处于应激状态,引发了肝功能损伤。 展开更多
关键词 亚急瘤胃酸中毒 生化指标 奶山羊 纤维碳水化合物与中洗涤纤维 组胺 脂多糖
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不同NFC/NDF水平饲粮对犊牛瘤胃发酵参数和微生物区系多样性的影响 被引量:38
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作者 李岚捷 成述儒 +5 位作者 刁其玉 付彤 毕研亮 王安思 李明 屠焰 《畜牧兽医学报》 CAS CSCD 北大核心 2017年第12期2347-2357,共11页
旨在研究不同非纤维性碳水化合物/中性洗涤纤维(NFC/NDF)水平饲粮对3~6月龄断奶公犊牛瘤胃发酵参数和微生物多样性的影响。选取2~3月龄健康、平均体重为(94.38±0.25)kg的断奶公犊牛30头,随机分为2组,每组15头。分别饲喂粗蛋白质水... 旨在研究不同非纤维性碳水化合物/中性洗涤纤维(NFC/NDF)水平饲粮对3~6月龄断奶公犊牛瘤胃发酵参数和微生物多样性的影响。选取2~3月龄健康、平均体重为(94.38±0.25)kg的断奶公犊牛30头,随机分为2组,每组15头。分别饲喂粗蛋白质水平相近,NFC/NDF分别为1.35(A组)和0.80(D组)的2种全混合饲粮。试验期105d,其中预试期15d,正试期90d。试验开始后,每组挑选6头体重相近、健康的犊牛,分别在120、150和180日龄晨饲后2h口腔采集瘤胃液,测定其pH、挥发性脂肪酸、氨态氮浓度和微生物多样性。结果表明:1)饲喂不同NFC/NDF水平饲粮对犊牛瘤胃液pH和总挥发性脂肪酸浓度无显著影响(P>0.05);2)NH3-N和乙酸浓度及乙/丙比A组显著低于D组(P<0.05),而丙酸浓度则A组显著高于D组(P<0.05);3)A组瘤胃微生物多样性指数皆显著低于D组(P<0.05);4)门水平上,拟杆菌门占序列总数的比例A组显著高于D组(P<0.05),属水平上Christensenellaceae_R-7和Prevotellaceae_UCG-003所占比例D组显著高于A组(P<0.05);5)随着日龄的增加,犊牛瘤胃液细菌多样性指数除Shannon指数外,其他指标皆组间差异显著(P<0.05),微生物组成无显著变化(P>0.05)。综上所述,饲喂不同NFC/NDF水平饲粮,显著影响了断奶犊牛瘤胃中微生物的种类和丰富度,影响属水平上微生物的组成,继而对乙酸和丙酸产量产生影响。 展开更多
关键词 断奶肉犊牛 纤维碳水化合物/中洗涤纤维 瘤胃发酵参数 瘤胃微生物区系
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不同NFC/NDF饲粮对青海藏羊育成母羊肌肉抗氧化功能、肌纤维类型组成及其相关基因表达的影响 被引量:10
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作者 周力 高占红 +4 位作者 张春梅 马博妍 李蒋伟 桂林生 侯生珍 《四川农业大学学报》 CSCD 北大核心 2021年第5期639-645,共7页
【目的】研究不同非纤维性碳水化合物(NFC)/中性洗涤纤维(NDF)饲粮对育成期藏羊(♀)背最长肌的肌纤维特性、肌球蛋白重链基因表达量及其抗氧化能力的影响。【方法】选用体况良好、体重(26.65±0.72)kg相近的4月龄青海藏羊母羔15只,... 【目的】研究不同非纤维性碳水化合物(NFC)/中性洗涤纤维(NDF)饲粮对育成期藏羊(♀)背最长肌的肌纤维特性、肌球蛋白重链基因表达量及其抗氧化能力的影响。【方法】选用体况良好、体重(26.65±0.72)kg相近的4月龄青海藏羊母羔15只,随机分为3组,进行预试期15 d和正试期90 d的饲养试验。正试期3个组饲粮NFC/NDF水平分别为0.92(L组)、1.39(M组)、2.20(H组)。以藏羊左侧胴体第12~13肋骨间背最长肌作为试验材料,利用酶联免疫吸附法(ELISA)、三磷酸腺苷酶(ATPase)染色和实时荧光定量PCR(real-time qPCR)方法进行测定。【结果】①M组Ⅰ型肌纤维直径小于H组(P<0.05)与L组(P>0.05);M组Ⅰ型肌纤维横截面积低于L组(P<0.05)与H组(P>0.05);M组Ⅰ型肌纤维密度高于H组(P<0.05)和L组(P>0.05);L组Ⅰ型肌纤维数量和面积显著小于M组(P<0.05),而Ⅱb型肌纤维数量和面积则相反(P<0.05)。②M组MyHCⅠ基因的表达量显著高于L组(P<0.05),而L组MyHCⅡa基因的表达量显著高于H组(P<0.05),同时L组MyHCⅡb和MyHCⅡx的基因的表达量显著高于M组(P<0.05)。③M组过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性大于L组(P<0.05)与H组(P>0.05);M组谷胱甘肽过氧化物酶(GSH-Px)活性大于H组(P<0.05)与L组(P>0.05)。【结论】不同NFC/NDF饲粮能够影响育成藏母羊肌肉抗氧化能力、肌纤维类型组成及其相关基因的表达量,其中以NFC/NDF为1.39的饲粮效果较佳。 展开更多
关键词 高原型藏羊 精粗比 纤维碳水化合物/中洗涤纤维 抗氧化能力 MyHCs基因
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Estimation of Digestible Energy Values of Plant Protein Supplement in Pig 被引量:1
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作者 李明元 王康宁 《Agricultural Science & Technology》 CAS 2009年第2期97-101,107,共6页
[Objective] The aim of the study was to establish the effective and accurate formulas for estimating the digestible energy (DE) values of plant protein supplement in pig. [Method] By difference method with different... [Objective] The aim of the study was to establish the effective and accurate formulas for estimating the digestible energy (DE) values of plant protein supplement in pig. [Method] By difference method with different amount of alternative feeds (20% -50%), two4 x4 Latin- square-designed trials were taken on eight castrated male pigs [ Yorkshire x Landrace x Neijiang pig, initial body-weight: (46 ±2) kg ] to deter- mine the apparent digestible energy (ADE) of the eight kinds of plant protein supplement commonly used in China, that is, corn gluten meal (sol.), soybean meal ( sol. ), fababean, pea, rapeseed meal ( sol. ), sesame meal ( sol. ), rapeseed meal ( exp. ) and cotton seed meal (sol.). [Resultl (1) Fiber was the most important factor to estimate the ADE of plant protein supplement in pigs, and ADF was the best one. (2) The most effective equations were as below: ( 1 ) OE (kJ/kg DM) = 14 741.86 - 185.01ADF+54.01SCHO+22.45CP ( R =0.988,RSD= 67.9,P〈0.01 ) ; (2) DE (kJ/kg DM) =22 223.26 -209.58ADF+26.79SCHO-1.09GE ( Ff =0.989,RSD=66.9, P〈0.01 ) . [Conclusion] The accurate, practical and specific regression equations were established for DE prediction of plant protein supplement in pig. 展开更多
关键词 Plant protein supplement PIG Digestible energy ESTIMATION
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不同peNDF与NFC/NDF组合日粮对绵羊采食行为、瘤胃发酵和营养物质表观消化率的影响 被引量:6
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作者 李美鑫 王可鑫 +3 位作者 徐晓东 姜宁 叶明 张爱忠 《中国畜牧杂志》 CAS 北大核心 2021年第6期192-197,共6页
本试验旨在研究混合日粮中不同物理有效中性洗涤纤维(peNDF)水平、非纤维性碳水化合物与中性洗涤纤维比值(NFC/NDF)对绵羊采食行为、瘤胃发酵和营养物质消化的影响,并初步探讨二者的互作效应。试验采用2×2交叉试验设计(日粮NFC/ND... 本试验旨在研究混合日粮中不同物理有效中性洗涤纤维(peNDF)水平、非纤维性碳水化合物与中性洗涤纤维比值(NFC/NDF)对绵羊采食行为、瘤胃发酵和营养物质消化的影响,并初步探讨二者的互作效应。试验采用2×2交叉试验设计(日粮NFC/NDF水平为0.63、0.80,peNDF水平为49.85%、53.64%),选取20只体重(35±1.3)kg的绵羊羯羊,随机分成4组,每组5个重复,每个重复1只羊。预试期14d,正试期7d。结果表明:peNDF对绵羊的采食、反刍及总咀嚼时间有显著影响;日粮NFC/NDF对绵羊的采食、反刍及总咀嚼时间无显著影响;随着peNDF水平升高,瘤胃pH、氨态氮、乙酸浓度、乙丙比升高(P<0.05),丙酸和丁酸浓度降低(P<0.05);随着NFC/NDF水平升高,瘤胃pH、乙酸和丁酸浓度、乙丙比降低(P<0.05),丙酸含量升高(P<0.05);peNDF对绵羊干物质的表观消化率有显著影响,日粮NFC/NDF水平对绵羊的干物质、有机物、粗蛋白质、中性洗涤纤维、酸性洗涤纤维表观消化率有显著影响;不同peNDF与NFC/NDF水平在绵羊咀嚼活动、瘤胃发酵和营养物质表观消化率的交互作用差异不显著。综上,在本次试验条件下,饲喂日粮peNDF为53.64%,NFC/NDF为0.63时,可促进绵羊的瘤胃发酵;日粮NFC/NDF为0.80、peNDF为49.85%时,可促进绵羊的营养物质消化能力。 展开更多
关键词 物理有效中洗涤纤维(peNDF) 纤维碳水化合物与中洗涤纤维的比(NFC/NDF) 采食行为 瘤胃发酵 营养物质表观消化率
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Leaf choice in black-and-white snub-nosed monkeys Rhinopithecus bieti is related to the physical and chemical properties of leaves 被引量:6
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作者 Zhipang HUANG Sheng HUO +2 位作者 Shuguo YANG Liangwei CUI Wen XIAO 《Current Zoology》 SCIE CAS CSCD 北大核心 2010年第6期643-649,共7页
To understand the effects of the chemical and physical properties of plant leaves on food choice in Rhinopithecus bieti, we collected mature leaves of nine food and five non-food plant species at the southernmost part... To understand the effects of the chemical and physical properties of plant leaves on food choice in Rhinopithecus bieti, we collected mature leaves of nine food and five non-food plant species at the southernmost part of the species's range. Chemical properties such as fat, ash, crude protein (CP), total phenolics (TP), and fiber content including neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin, celluloses and hemicelluloses, and physical toughness were measured. R. bieti tended to choose leaves with lower fiber content, higher ash, a higher ratio of CP/ADF, and lower toughness. No difference was found for fat, crude protein, total phenolics, hemicelluloses and lignin between food and non-food leaves. Even though the ratio of CP/ADF is generally regarded as a good indicator for colobine food choice, the difference in the ratio of CP/ADF between food and non-food leaves was only the result of differences in ADE Since positive correlations were found between ADF and tough- ness from all leaves (both food and non-food species), and toughness of leaves was likely easier for R. bieti to sense than fiber content via mastication, the toughness of leaves may function as a predictor of food choice in this species [Current Zoology 56 (6) 643-49, 2010]. 展开更多
关键词 Rhinopithecus bieti Diet selection Protein-to-fiber ratio TOUGHNESS Mt. Longma YUNNAN
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Prediction of net energy value of corn and soybean meal for broiler chicken 被引量:4
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作者 HUAN Zong-jin WANG Kang-ning 《Journal of Agricultural Science and Technology》 2009年第6期6-12,共7页
Net energy (NE) is the most accurate and unbiased way in characterizing the energy content of feed. Comparatively speaking, research on NE prediction is still limited and under development. Therefore, it is importan... Net energy (NE) is the most accurate and unbiased way in characterizing the energy content of feed. Comparatively speaking, research on NE prediction is still limited and under development. Therefore, it is important to correctly assess NE for further study. In this study, an energy balance trial and a fasting metabolism trial were used to measure the net energy for production (NEp) and the net energy for maintenance (NEm), respectively. Meanwhile, a backward method of multiple linear regression was applied to establish prediction equations for NE. The results showed that corn NE is about 2 times higher than that of soybean meal, due to the efficiency of ME (k) utilization by the ingredients. Meanwhile, AME and acid detergent fiber (ADF) were found to be better predictors for the NE of corn and soybean meal, respectively. Furthermore, Equations 1(R^2=0.993, RSD=0.0091) and 12 (R^2=0.999, RSD=0.0008) were found to be better prediction equations in multiple linear regression analyses for corn and soybean meal, respectively. However, considering the cost of the analytical procedures, the accuracy of the prediction (RSD), and the additional variability or errors due to interlaboratory variance, Equation 4 (R^2=0.990, RSD=0.0109) and Equation 20 (R^2=0.996, RSD=0.0043) were better models for corn and soybean meal, respectively. Nevertheless, these equations for corn and soybean meal should be applied only under similar experimental conditions. 展开更多
关键词 net energy CORN soybean meal CHICKEN prediction equatton
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Effect of Raw Material for Distillers Grains Production on Protein Quality for Ruminants
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作者 Máia Chrenková Zuzana Ceresnakova Zuzana Formelova Maria Polacikova Pavol Frak 《Journal of Life Sciences》 2011年第10期814-820,共7页
The present experiment was focused on quality of dry distillers' grains with solubles (DDGS) produced from corn (DDGSc), wheat (DDGSw), triticale (DDGSt), and wet distillers grains from corn (WDGc) for rumi... The present experiment was focused on quality of dry distillers' grains with solubles (DDGS) produced from corn (DDGSc), wheat (DDGSw), triticale (DDGSt), and wet distillers grains from corn (WDGc) for ruminants. The products were tested for dry matter (DM), crude protein (CP), neutral detergent fibre (NDF), acid detergent fibre (ADF) acid detergent insoluble nitrogen (NIAD), amino acid (AA) profile, CP degradability and intestinal digestibility (ID) of rumen undegraded protein (RUP). The average content of CP (g/kg DM) ranged from 289.9 (DDGSc) to 356.9 (DDGSw). The highest content of essential AA was in WDGc (440.1 g/160g N) followed with DDGSc. The most variable was the content of Lys in DDGSw (from 13.69 to 22.79 g/160 g N). The NIAD was found the highest for DDGSw (from 16.8% to 36.2%), effective CP degradability was only 50.2% and ID of RUP 82.2%, total essential AA 81.2%, Lys 77.69% and Met 81.4%. Lys was generally the least digestible AA. The ruminally degradable CP fraction was in the range from 52.6% to 70.2% for DDGSc. ID of total and essential by-pass AA was very high 96.2% and 96.1%, Lysine 90.9% and Methionine 96.7%. From these data it can be concluded that DDGS from grains are a good source of protein for ruminants. 展开更多
关键词 DDGS crude protein amino acids in situ degradability intestinal digestibility.
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The Effect of Clove Essential Oil Treatment on the Cell Wall Components of Wheat Straw
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作者 Hulya Ozelcam Sema Ozuretmen +1 位作者 Hasan Huseyin Ipcak Aylin Dereboylu 《Journal of Agricultural Science and Technology(A)》 2017年第1期68-72,共5页
The aim of this study was to determine the effects of additions of different doses of clove oil (Syzygium aromaticum L.) on cell wall component of wheat straw. For this purpose, wheat straw was treated with 100 ppm ... The aim of this study was to determine the effects of additions of different doses of clove oil (Syzygium aromaticum L.) on cell wall component of wheat straw. For this purpose, wheat straw was treated with 100 ppm and 200 ppm clove oil and applied at two different time period (1 h and 5 h). The microscopic analysis was made on cell wall components of untreated and treated of the straw. According to the research findings, with increasing doses and time of clove oil treatment, particularly, neutral detergent fiber (NDF) and acid detergent fiber (ADF) content of straw significantly (P 〈 0.05) reduced, approximately at the level of 15% for NDF and 13% for ADF, respectively. The lowest NDF, ADF, acid detergent lignin (ADL) and cellulose contents were found in 200 ppm dose and 5 h period. However, the lowest stem section thickness likewise was determined in 5 h period (P 〈 0.05), but there was no significant difference between the dose. Consequently, it could be said that the addition of clove oil have a positive influence on cell wall components and stem section thickness of wheat straw. 展开更多
关键词 Wheat straw clove oil cell wall components.
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Nutritional Value Evaluation of Saccharum spontaneum L. Germplasm Resources
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作者 Jianle LIU Changjun BAI +3 位作者 Linling YAN Shimeng CHEN Yu ZHANG Hubiao YANG 《Agricultural Science & Technology》 CAS 2014年第11期1981-1986,2002,共7页
The contents of dry matter, crude fat, crude protein, neutral detergent fiber, acid detergent fiber, crude ash, Ca and P in 43 Saccharum spontaneum L.germplasms during the vegetative period were determined. Among them... The contents of dry matter, crude fat, crude protein, neutral detergent fiber, acid detergent fiber, crude ash, Ca and P in 43 Saccharum spontaneum L.germplasms during the vegetative period were determined. Among them, the crude protein, crude fat, neutral detergent fiber, acid detergent fiber, P and Ca contents were treated as the judging indicators. The nutritional value evaluation was carried out with the analytic hierarchy process(AHP). The results showed A49(Guangxi)had the highest nutritional value. Its dry matter, crude protein, crude fat, crude ash,neutral detergent fiber, acid detergent fiber, Ca and P contents were 32.35%,7.20%, 4.06%, 8.07%, 75.81%, 50.72%, 0.23% and 0.16% respectively. While A3(Guangdong) had the lowest nutritional value. Its dry matter, crude protein, crude fat, crude ash, neutral detergent fiber, acid detergent fiber, Ca and P contents were32.24%, 4.62%, 0.51%, 6.38%, 46.40%, 40.02%, 0.15% and 0.09% respectively.The crude protein content differed significantly among different germplasms. A48 had the highest crude protein content(9.11%), and A14 had the lowest crude protein content(3.72%). Based on the evaluation results, the 43 Saccharum spontaneum L.germplasms were divided into 3 groups: high-nutritional value type, moderate-nutritional value type and low-nutritional value type. We hoped to provide a theoretical reference for the application of Saccharum spontaneum L. as a forage grass. 展开更多
关键词 Saccharum spontaneum L. Neutral detergent fiber Nutritional value Evaluation
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Native Trees Forage Alternative Protein Feed for Cattle in Tropical "Mexico"
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作者 Amalia Cabrera-Nunez Pablo Elorza-Martinez Arturo Serranc Iliana Del Carmen Daniel Renteria MiguelAngel Lammoglia Villagomez 《Journal of Environmental Science and Engineering(B)》 2012年第3期350-353,共4页
The objective of this study was to find highly nutritious native plants to feed cattle in northern Veracruz (Mexico) as an alternative to avoid deforestation for pasture establishment. Six fodder tree species (Leuc... The objective of this study was to find highly nutritious native plants to feed cattle in northern Veracruz (Mexico) as an alternative to avoid deforestation for pasture establishment. Six fodder tree species (Leucaena, Leucaenaleucocephala, Morera, Morus alba, Chacloco, rubiHamelia patents, Guficima, Guasumaulmifolia, Pichoco-bunting, Erythrinaamericana, Cocuite, Gliricidiasepium) were selected for their leave production, rapid growth and high nutritional quality. The plants were evaluated in the bud stage of senescence and flowering, restricting the fraction of mature foliage in the samples located under 2 meters height. Samples were collected from five plants per specie that were randomly selected. The nutritious parameters were evaluated through proximate analysis with the Van Soest technique. This study used a completely randomized design with five replicates. The nutritious composition showed PC (protein content) differences (P 〈 0.05) among species indicating that Leucaenaleucocephala (20%) had the lowest, Gliricidiasepium was (21%) intermediate and Morus alba (23%) had the highest. The FDN (neutral detergent fiber) and FDA (acid detergent) were lowest for Guasumaulmifolia (23.4%) and the other five species exceeded 40%. In conclusion, native foliage of fodder trees in northern, Veracruz are highly nutritious for cattle feeding and could alternative to avoid deforestation for pasture establishment to continue. 展开更多
关键词 Fodder trees nutritional value CATTLE deforestation.
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Vegetation Resources and Goat Foraging Behaviour in Rangeland System in Southern of Madagascar
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作者 Arsene Jules Randrianariveloseheno Jean de Neupomuscene Rakotozandriny Roger Daccord 《Journal of Life Sciences》 2014年第11期902-907,共6页
Goat satisfying energy requirements of goat are determined by anatomical body and browse leaves. In High Bassin zone of Mandrare in Southern Madagascar, sampling area of 400 m^2 has been monitored to analysis floristi... Goat satisfying energy requirements of goat are determined by anatomical body and browse leaves. In High Bassin zone of Mandrare in Southern Madagascar, sampling area of 400 m^2 has been monitored to analysis floristic diversity and Shannon-Weaver index and to establish rangeland typology. With one ram, two castrated goats and three ewes, foraging behaviour, bite rate and forage nutritive value were analyzed in native pastureland during six days at two browsing periods. As results, four pastureland groups were classified with dominant shrubs plants Acacia farnesiana, Poupartia caffra, Kigelianthe madagascariensis and Rhigozum madagascariense. Rangelands sites have presented lower heterogeneity with floristic density (236-658 plants per hectare), Shannon-Weaver index (0.28-0.95), maximum diversity (1.04 ± 0.07) with P 〈 0.05. Most of 42 identified fodder plants presented significant difference (P 〈 0.05) and higher nutritive value (35-54% Dry Matter or DM); mineral matter (70-162 g·kg DM^-1); Crude Protein (124-175 g·kg DM^-1); Crude Fibre (203-376 g·kg DM^-1); Neutral Detergent Fiber (388-548 g·kg DM^-1) and gross energy (3617-4464 kcal·kg DM^-1). Acaciafarnesiana rangeland was overgrazed with 4.00 to 9.00 bites·mn^-1. In fact, relationships between phytosociology, nutritional parameters and foraging behaviour have been analyzed to improve with autochthonous plants and to manage rangeland in semi arid environment. 展开更多
关键词 BITE goats FORAGING BEHAVIOUR Madagascar.
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Intake and Digestibility of Native and Exotic Grasses Fed Ad libitum to Djallonke Sheep in South Benin
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作者 S. Babatounde R. Glele KaKai +1 位作者 I. Alkoiret G. A. Mensah 《Journal of Agricultural Science and Technology》 2011年第4期513-524,共12页
Native (Andropogon gayanus, Panicum maximum and Pennisetum purpureum) and exotic grasses (Brachiaria ruzi:iensis, Panieum maximum cv. C1 and T58) consumed in tropical humid zone were evaluated by estimating their... Native (Andropogon gayanus, Panicum maximum and Pennisetum purpureum) and exotic grasses (Brachiaria ruzi:iensis, Panieum maximum cv. C1 and T58) consumed in tropical humid zone were evaluated by estimating their voluntary intake and in vivo digestibility. The measurements were performed with 4 male Djallonke sheep (28 kg LW) fed ad libitum in metabolic cages, during a 10-days in vivo trial, preceded by a 14-days accommodation period. Each grass was studied at 3 stages of development. The samples (distributed grasses, refusals and faeces) were analysed for organic matter (OM), crude fibre (CF), neutral detergent fibre (NDF), acid detergent fibre (ADF), acid detergent lignin (ADL) and crude protein (CP) contents. Voluntary digestible organic matter intake (VDOMI) and voluntary digestible crude protein intake (VDCPI) were calculated (in g kg ^-1 LW075) as synthetic parameters to compare the grasses. The chemical composition of the distributed forages varied significantly (P 〈 0.001) between species or cultivars and development stages. After 4-5 leaves per tiller stage, CP contents decreased in all species and lowest in native species. Andropogon gayanus was the most l ignified species at any stage of development. At the end of the rainy season, Pennisetum purpureum leaves and Brachiaria ruziziensis had the highest CP. For all the grasses, CP contents were higher (and CF contents lower) in the consumed material, compared to the offered forages, showing thereby the high selectivity by the sheep. Dry matter intake, OM and CP digestibility varied greatly (P 〈 0.001) between forages and development stages with high interactions between these factors (P 〈 0.001). Voluntary digestible organic matter intake and VDCPI were the highest (P 〈 0.05) for native Panicum maximum and Pennisetum purpureum. There was a relationship between digestibility and intake parameters, and ADL content of the grasses seems to be an important factor determining at least digestibility. 展开更多
关键词 Tropical grasses INTAKE DIGESTIBILITY Djallonke sheep humid tropics.
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Effects of Multi-enzyme Preparations on Growth Performance, Apparent Total Tract Digestibility and In Vitro Digestibility for Growing Pigs
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作者 D. C. Liu Z. S. Wang +1 位作者 W. Qiao A. G. Zhou 《Journal of Agricultural Science and Technology》 2010年第4期18-25,共8页
The aim of this study was to investigate the effects of enzyme supplementations on performance of growing pigs, apparent total tract digestibility (ATTD) and in vitro digestibility (IVD) of nutrients. 224 growing ... The aim of this study was to investigate the effects of enzyme supplementations on performance of growing pigs, apparent total tract digestibility (ATTD) and in vitro digestibility (IVD) of nutrients. 224 growing pigs weighing 30-4-1.2 kg were allocated to fed basal diets supplemented with 0, 0.1, 0.15 or 0.2 g/kg enzyme preparations in growth trail. Those four diets were used for in vio'o digestibility study. Pigs fed enzyme-supplemented diets improved average daily gain and decreased ratio of feed/gain (P〈0.05). Enzyme supplementations increased ATTD of dry matter, energy, crude protein, neutral detergent fiber, acid detergent fibre and hemicellulose (P〈0.05). Supplementation with enzymes improved reducing sugar production and the IVD of dry matter, crude protein, neutral detergent fiber, acid detergent fibre, total arabinoxylans, galacturonic acid, hemicellulose and cellulose. 0.015% enzyme supplementation in diets seemed to be sufficient to achieve maximum growth performance, nutrients digestibilities and reducing sugar production. Collectively, these results suggested that the supplementation of enzymes in diet increased the digestibilities of nutrients through the promotion of NSP degradation, thereby enhancing growth performance of growing pigs. 展开更多
关键词 DIGESTIBILITY in vitro ENZYME growing pig growth performance
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