To understand the effects of the chemical and physical properties of plant leaves on food choice in Rhinopithecus bieti, we collected mature leaves of nine food and five non-food plant species at the southernmost part...To understand the effects of the chemical and physical properties of plant leaves on food choice in Rhinopithecus bieti, we collected mature leaves of nine food and five non-food plant species at the southernmost part of the species's range. Chemical properties such as fat, ash, crude protein (CP), total phenolics (TP), and fiber content including neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin, celluloses and hemicelluloses, and physical toughness were measured. R. bieti tended to choose leaves with lower fiber content, higher ash, a higher ratio of CP/ADF, and lower toughness. No difference was found for fat, crude protein, total phenolics, hemicelluloses and lignin between food and non-food leaves. Even though the ratio of CP/ADF is generally regarded as a good indicator for colobine food choice, the difference in the ratio of CP/ADF between food and non-food leaves was only the result of differences in ADE Since positive correlations were found between ADF and tough- ness from all leaves (both food and non-food species), and toughness of leaves was likely easier for R. bieti to sense than fiber content via mastication, the toughness of leaves may function as a predictor of food choice in this species [Current Zoology 56 (6) 643-49, 2010].展开更多
The objective of this study was to find highly nutritious native plants to feed cattle in northern Veracruz (Mexico) as an alternative to avoid deforestation for pasture establishment. Six fodder tree species (Leuc...The objective of this study was to find highly nutritious native plants to feed cattle in northern Veracruz (Mexico) as an alternative to avoid deforestation for pasture establishment. Six fodder tree species (Leucaena, Leucaenaleucocephala, Morera, Morus alba, Chacloco, rubiHamelia patents, Guficima, Guasumaulmifolia, Pichoco-bunting, Erythrinaamericana, Cocuite, Gliricidiasepium) were selected for their leave production, rapid growth and high nutritional quality. The plants were evaluated in the bud stage of senescence and flowering, restricting the fraction of mature foliage in the samples located under 2 meters height. Samples were collected from five plants per specie that were randomly selected. The nutritious parameters were evaluated through proximate analysis with the Van Soest technique. This study used a completely randomized design with five replicates. The nutritious composition showed PC (protein content) differences (P 〈 0.05) among species indicating that Leucaenaleucocephala (20%) had the lowest, Gliricidiasepium was (21%) intermediate and Morus alba (23%) had the highest. The FDN (neutral detergent fiber) and FDA (acid detergent) were lowest for Guasumaulmifolia (23.4%) and the other five species exceeded 40%. In conclusion, native foliage of fodder trees in northern, Veracruz are highly nutritious for cattle feeding and could alternative to avoid deforestation for pasture establishment to continue.展开更多
Native (Andropogon gayanus, Panicum maximum and Pennisetum purpureum) and exotic grasses (Brachiaria ruzi:iensis, Panieum maximum cv. C1 and T58) consumed in tropical humid zone were evaluated by estimating their...Native (Andropogon gayanus, Panicum maximum and Pennisetum purpureum) and exotic grasses (Brachiaria ruzi:iensis, Panieum maximum cv. C1 and T58) consumed in tropical humid zone were evaluated by estimating their voluntary intake and in vivo digestibility. The measurements were performed with 4 male Djallonke sheep (28 kg LW) fed ad libitum in metabolic cages, during a 10-days in vivo trial, preceded by a 14-days accommodation period. Each grass was studied at 3 stages of development. The samples (distributed grasses, refusals and faeces) were analysed for organic matter (OM), crude fibre (CF), neutral detergent fibre (NDF), acid detergent fibre (ADF), acid detergent lignin (ADL) and crude protein (CP) contents. Voluntary digestible organic matter intake (VDOMI) and voluntary digestible crude protein intake (VDCPI) were calculated (in g kg ^-1 LW075) as synthetic parameters to compare the grasses. The chemical composition of the distributed forages varied significantly (P 〈 0.001) between species or cultivars and development stages. After 4-5 leaves per tiller stage, CP contents decreased in all species and lowest in native species. Andropogon gayanus was the most l ignified species at any stage of development. At the end of the rainy season, Pennisetum purpureum leaves and Brachiaria ruziziensis had the highest CP. For all the grasses, CP contents were higher (and CF contents lower) in the consumed material, compared to the offered forages, showing thereby the high selectivity by the sheep. Dry matter intake, OM and CP digestibility varied greatly (P 〈 0.001) between forages and development stages with high interactions between these factors (P 〈 0.001). Voluntary digestible organic matter intake and VDCPI were the highest (P 〈 0.05) for native Panicum maximum and Pennisetum purpureum. There was a relationship between digestibility and intake parameters, and ADL content of the grasses seems to be an important factor determining at least digestibility.展开更多
The contents of dry matter, crude fat, crude protein, neutral detergent fiber, acid detergent fiber, crude ash, Ca and P in 43 Saccharum spontaneum L.germplasms during the vegetative period were determined. Among them...The contents of dry matter, crude fat, crude protein, neutral detergent fiber, acid detergent fiber, crude ash, Ca and P in 43 Saccharum spontaneum L.germplasms during the vegetative period were determined. Among them, the crude protein, crude fat, neutral detergent fiber, acid detergent fiber, P and Ca contents were treated as the judging indicators. The nutritional value evaluation was carried out with the analytic hierarchy process(AHP). The results showed A49(Guangxi)had the highest nutritional value. Its dry matter, crude protein, crude fat, crude ash,neutral detergent fiber, acid detergent fiber, Ca and P contents were 32.35%,7.20%, 4.06%, 8.07%, 75.81%, 50.72%, 0.23% and 0.16% respectively. While A3(Guangdong) had the lowest nutritional value. Its dry matter, crude protein, crude fat, crude ash, neutral detergent fiber, acid detergent fiber, Ca and P contents were32.24%, 4.62%, 0.51%, 6.38%, 46.40%, 40.02%, 0.15% and 0.09% respectively.The crude protein content differed significantly among different germplasms. A48 had the highest crude protein content(9.11%), and A14 had the lowest crude protein content(3.72%). Based on the evaluation results, the 43 Saccharum spontaneum L.germplasms were divided into 3 groups: high-nutritional value type, moderate-nutritional value type and low-nutritional value type. We hoped to provide a theoretical reference for the application of Saccharum spontaneum L. as a forage grass.展开更多
In the past few years there has been a growth in the use of nanoparticles for stabilizing lipid membranes that contain embedded proteins. These bionanoparticles provide a solution to the challenging problem of membran...In the past few years there has been a growth in the use of nanoparticles for stabilizing lipid membranes that contain embedded proteins. These bionanoparticles provide a solution to the challenging problem of membrane protein isolation by maintaining a lipid bilayer essential to protein integrity and activity. We have previously described the use of an amphipathic polymer (poly(styrene-co-maleic add), SMA) to produce discoidal nanoparticles with a lipid bilayer core containing the embedded protein. However the structure of the nanoparticle itself has not yet been determined. This leaves a major gap in understanding how the SMA stabilizes the encapsulated bilayer and how the bilayer relates physically and structurally to an unencapsulated lipid bilayer. In this paper we address this issue by describing the structure of the SMA lipid particle (SMALP) using data from small angle neutron scattering (SANS), electron microscopy (EM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC) and nuclear magnetic resonance spectroscopy (NMR). We show that the particle is disc shaped containing a polymer "bracelet" encircling the lipid bilayer. The structure and orientation of the individual components within the bilayer and polymer are determined showing that styrene moieties within SMA intercalate between the lipid acyl chains. The dimensions of the encapsulated bilayer are also determined and match those measured for a natural membrane. Taken together, the description of the structure of the SMALP forms the foundation for future development and applications of SMALPs in membrane protein production and analysis.展开更多
基金supported by TNC "Conservation Ecology of Yunnan Snub-nosed Monkey (Rhinopithecus bieti) at Two Geographic Extremes of Species Range"PhD Funding from Dali University,National Natural Science Foundation of China (No.30770308,30960084,30960085)+1 种基金Key Laboratory of Forest Disaster Warning and Control in Yunnan Province (ZK09A313)Key Subject of Wildlife Conservation and Utilization in Yunnan Province (XKZ200904)
文摘To understand the effects of the chemical and physical properties of plant leaves on food choice in Rhinopithecus bieti, we collected mature leaves of nine food and five non-food plant species at the southernmost part of the species's range. Chemical properties such as fat, ash, crude protein (CP), total phenolics (TP), and fiber content including neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin, celluloses and hemicelluloses, and physical toughness were measured. R. bieti tended to choose leaves with lower fiber content, higher ash, a higher ratio of CP/ADF, and lower toughness. No difference was found for fat, crude protein, total phenolics, hemicelluloses and lignin between food and non-food leaves. Even though the ratio of CP/ADF is generally regarded as a good indicator for colobine food choice, the difference in the ratio of CP/ADF between food and non-food leaves was only the result of differences in ADE Since positive correlations were found between ADF and tough- ness from all leaves (both food and non-food species), and toughness of leaves was likely easier for R. bieti to sense than fiber content via mastication, the toughness of leaves may function as a predictor of food choice in this species [Current Zoology 56 (6) 643-49, 2010].
文摘The objective of this study was to find highly nutritious native plants to feed cattle in northern Veracruz (Mexico) as an alternative to avoid deforestation for pasture establishment. Six fodder tree species (Leucaena, Leucaenaleucocephala, Morera, Morus alba, Chacloco, rubiHamelia patents, Guficima, Guasumaulmifolia, Pichoco-bunting, Erythrinaamericana, Cocuite, Gliricidiasepium) were selected for their leave production, rapid growth and high nutritional quality. The plants were evaluated in the bud stage of senescence and flowering, restricting the fraction of mature foliage in the samples located under 2 meters height. Samples were collected from five plants per specie that were randomly selected. The nutritious parameters were evaluated through proximate analysis with the Van Soest technique. This study used a completely randomized design with five replicates. The nutritious composition showed PC (protein content) differences (P 〈 0.05) among species indicating that Leucaenaleucocephala (20%) had the lowest, Gliricidiasepium was (21%) intermediate and Morus alba (23%) had the highest. The FDN (neutral detergent fiber) and FDA (acid detergent) were lowest for Guasumaulmifolia (23.4%) and the other five species exceeded 40%. In conclusion, native foliage of fodder trees in northern, Veracruz are highly nutritious for cattle feeding and could alternative to avoid deforestation for pasture establishment to continue.
文摘Native (Andropogon gayanus, Panicum maximum and Pennisetum purpureum) and exotic grasses (Brachiaria ruzi:iensis, Panieum maximum cv. C1 and T58) consumed in tropical humid zone were evaluated by estimating their voluntary intake and in vivo digestibility. The measurements were performed with 4 male Djallonke sheep (28 kg LW) fed ad libitum in metabolic cages, during a 10-days in vivo trial, preceded by a 14-days accommodation period. Each grass was studied at 3 stages of development. The samples (distributed grasses, refusals and faeces) were analysed for organic matter (OM), crude fibre (CF), neutral detergent fibre (NDF), acid detergent fibre (ADF), acid detergent lignin (ADL) and crude protein (CP) contents. Voluntary digestible organic matter intake (VDOMI) and voluntary digestible crude protein intake (VDCPI) were calculated (in g kg ^-1 LW075) as synthetic parameters to compare the grasses. The chemical composition of the distributed forages varied significantly (P 〈 0.001) between species or cultivars and development stages. After 4-5 leaves per tiller stage, CP contents decreased in all species and lowest in native species. Andropogon gayanus was the most l ignified species at any stage of development. At the end of the rainy season, Pennisetum purpureum leaves and Brachiaria ruziziensis had the highest CP. For all the grasses, CP contents were higher (and CF contents lower) in the consumed material, compared to the offered forages, showing thereby the high selectivity by the sheep. Dry matter intake, OM and CP digestibility varied greatly (P 〈 0.001) between forages and development stages with high interactions between these factors (P 〈 0.001). Voluntary digestible organic matter intake and VDCPI were the highest (P 〈 0.05) for native Panicum maximum and Pennisetum purpureum. There was a relationship between digestibility and intake parameters, and ADL content of the grasses seems to be an important factor determining at least digestibility.
基金Supported by the Tropical Forage Grass Germplasm Conservation Project of Ministry of Agriculture(2011BAD17B01-01-5)Modern Agriculture Talent Support Program(No.PZS083)Tropical Crops Germplasm Conservation Project(12RZZY-09)
文摘The contents of dry matter, crude fat, crude protein, neutral detergent fiber, acid detergent fiber, crude ash, Ca and P in 43 Saccharum spontaneum L.germplasms during the vegetative period were determined. Among them, the crude protein, crude fat, neutral detergent fiber, acid detergent fiber, P and Ca contents were treated as the judging indicators. The nutritional value evaluation was carried out with the analytic hierarchy process(AHP). The results showed A49(Guangxi)had the highest nutritional value. Its dry matter, crude protein, crude fat, crude ash,neutral detergent fiber, acid detergent fiber, Ca and P contents were 32.35%,7.20%, 4.06%, 8.07%, 75.81%, 50.72%, 0.23% and 0.16% respectively. While A3(Guangdong) had the lowest nutritional value. Its dry matter, crude protein, crude fat, crude ash, neutral detergent fiber, acid detergent fiber, Ca and P contents were32.24%, 4.62%, 0.51%, 6.38%, 46.40%, 40.02%, 0.15% and 0.09% respectively.The crude protein content differed significantly among different germplasms. A48 had the highest crude protein content(9.11%), and A14 had the lowest crude protein content(3.72%). Based on the evaluation results, the 43 Saccharum spontaneum L.germplasms were divided into 3 groups: high-nutritional value type, moderate-nutritional value type and low-nutritional value type. We hoped to provide a theoretical reference for the application of Saccharum spontaneum L. as a forage grass.
文摘In the past few years there has been a growth in the use of nanoparticles for stabilizing lipid membranes that contain embedded proteins. These bionanoparticles provide a solution to the challenging problem of membrane protein isolation by maintaining a lipid bilayer essential to protein integrity and activity. We have previously described the use of an amphipathic polymer (poly(styrene-co-maleic add), SMA) to produce discoidal nanoparticles with a lipid bilayer core containing the embedded protein. However the structure of the nanoparticle itself has not yet been determined. This leaves a major gap in understanding how the SMA stabilizes the encapsulated bilayer and how the bilayer relates physically and structurally to an unencapsulated lipid bilayer. In this paper we address this issue by describing the structure of the SMA lipid particle (SMALP) using data from small angle neutron scattering (SANS), electron microscopy (EM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC) and nuclear magnetic resonance spectroscopy (NMR). We show that the particle is disc shaped containing a polymer "bracelet" encircling the lipid bilayer. The structure and orientation of the individual components within the bilayer and polymer are determined showing that styrene moieties within SMA intercalate between the lipid acyl chains. The dimensions of the encapsulated bilayer are also determined and match those measured for a natural membrane. Taken together, the description of the structure of the SMALP forms the foundation for future development and applications of SMALPs in membrane protein production and analysis.