目的利用CiteSpace软件分析水蛭在医药方面的文献,了解水蛭相关研究的现状、热点和发展趋势,为水蛭领域的研究学者提供参考。方法系统检索1996—2022年CNKI和Web of Science数据库中收录的水蛭在医药研究领域的相关文献,运用CiteSpace 6...目的利用CiteSpace软件分析水蛭在医药方面的文献,了解水蛭相关研究的现状、热点和发展趋势,为水蛭领域的研究学者提供参考。方法系统检索1996—2022年CNKI和Web of Science数据库中收录的水蛭在医药研究领域的相关文献,运用CiteSpace 6.1R3软件对其发文量、作者、研究机构、国家,关键词共现、聚类、突现等进行文献计量和可视化分析。结果共纳入CNKI数据库中文文献1115篇以及Web ofScience数据库英文文献237篇文献。中英文文献分析表明,作者史红专中文文献发文量最多,作者Hildebrandt英文发文量最多;山东中医药大学和中国科学院是分别是中、英发文量最多的机构;发表英文文献最多的国家为美国。中英文关键词共现及聚类分析显示,中文文献注重研究水蛭的有效成分水蛭素,包括分子结构、药理药效,以及治疗血栓、冠心病、肾病等疾病的作用机制等,同时关注不同种类水蛭活性成分的差异;英文文献除此之外还注重研究活体水蛭的临床外用。关键词突现提示,作用机制、水蛭素类似物的寻找和合成、配伍应用时的药效机制等不仅是目前的研究热点,也可能是未来的热点方向。结论水蛭成分分析、水蛭治疗心血管疾病、慢性肾脏病等疾病的药理作用机制研究始终是本领域研究热点;水蛭素类似物、配伍应用的药效机制、网络药理、分子对接等可能是水蛭的未来研究热点和趋势。展开更多
As outdoor sports are loved by more and more people, the introduction of outdoor sports to the EE. teaching practice plays a positive role in enriching the contents of student sports activities. In this paper, the mea...As outdoor sports are loved by more and more people, the introduction of outdoor sports to the EE. teaching practice plays a positive role in enriching the contents of student sports activities. In this paper, the meanings and characteristics of outdoor sports are introduced, the necessities for the outdoor RE. teaching practice are analyzed, and finally several suggestions on the construction of the outdoor teaching practice in physical education are proposed.展开更多
Objective THANK, known as a member of TNF superfamily, is a potent costimulator of both B and T lymphocytes and can promote astrong immune response. To investigate its role in liver immunotherapy, the anti-tumor effec...Objective THANK, known as a member of TNF superfamily, is a potent costimulator of both B and T lymphocytes and can promote astrong immune response. To investigate its role in liver immunotherapy, the anti-tumor effects of the THANK-transduced hepatoma cellline SMMU-7721 in vitro and in vivo were studied.Methods THANK full-length cDNA was transfected into SMMU-7721 cell line. The transfectant with stable expression of THANK wasobtained by clone selection and THANK s effects on hepatoma cells were analyzed, further the tumorigenicity of THANK-transduced7721 cells was examined in nude mice.Results THANK's expression in 7721 cells inhibited the growth of hepatoma cells and induced a strong CTL response in vitro. The cellcycle analysis showed that THANK transfected 7721 cells were arrested in the S phase. The expression of THANK in SMMU-7721 cellline not only inhibited the tumorigenicity of 7721 cells, but also induced a systemic immune response against re-challenge of parental7721 tumors.Conclusion THANK transduction in SMMU-7721 cells can induce an effective immune response in nude mice and may be useful for theimmunotherapy of hepatomas.展开更多
Neutralizing antibodies are considered to be an important protective parameter used in HIV-1 vaccine evaluation. However, the exact role that neutralizing antibodies plays in controlling the disease progression of HIV...Neutralizing antibodies are considered to be an important protective parameter used in HIV-1 vaccine evaluation. However, the exact role that neutralizing antibodies plays in controlling the disease progression of HIV-1 infected peoples is still undetermined. In this paper, we compared the protective function of the neutralizing antibody response in the plasma from LTNP and TP against clade B and clade C pseudoviruses. No difference in the neutralizing activities between the plasma from LTNP and TP was found, which was consistent with the most recent reports. In addition, no correlations between the titer or breadth and CD4+ or viral load in HIV-1 infected individuals were found. The protective roles played by neutralizing antibodies in controlling disease progression of HIV-1 infected people need to be considered in a new viewpoint.展开更多
The combination of magnetotelluric survey and laboratory measurements of electrical conductivity is a powerful approach for exploring the conditions of Earth's deep interior. Electrical conductivity of hydrous sil...The combination of magnetotelluric survey and laboratory measurements of electrical conductivity is a powerful approach for exploring the conditions of Earth's deep interior. Electrical conductivity of hydrous silicate melts and aqueous fluids is sensitive to composition, temperature, and pressure, making it useful for understanding partial melting and fluid activity at great depths. This study presents a review on the experimental studies of electrical conductivity of silicate melts and aqueous fluids, and introduces some important applications of experimental results. For silicate melts, electrical conductivity increases with increasing temperature but decreases with pressure. With a similar Na^+ concentration, along the calc-alkaline series electrical conductivity generally increases from basaltic to rhyolitic melt, accompanied by a decreasing activation enthalpy. Electrical conductivity of silicate melts is strongly enhanced with the incorporation of water due to promoted cation mobility. For aqueous fluids, research is focused on dilute electrolyte solutions. Electrical conductivity typically first increases and then decreases with increasing temperature, and increases with pressure before approaching a plateau value. The dissociation constant of electrolyte can be derived from conductivity data. To develop generally applicable quantitative models of electrical conductivity of melt/fluid addressing the dependences on temperature, pressure, and composition, it requires more electrical conductivity measurements of representative systems to be implemented in an extensive P-T range using up-to-date methods.展开更多
文摘目的利用CiteSpace软件分析水蛭在医药方面的文献,了解水蛭相关研究的现状、热点和发展趋势,为水蛭领域的研究学者提供参考。方法系统检索1996—2022年CNKI和Web of Science数据库中收录的水蛭在医药研究领域的相关文献,运用CiteSpace 6.1R3软件对其发文量、作者、研究机构、国家,关键词共现、聚类、突现等进行文献计量和可视化分析。结果共纳入CNKI数据库中文文献1115篇以及Web ofScience数据库英文文献237篇文献。中英文文献分析表明,作者史红专中文文献发文量最多,作者Hildebrandt英文发文量最多;山东中医药大学和中国科学院是分别是中、英发文量最多的机构;发表英文文献最多的国家为美国。中英文关键词共现及聚类分析显示,中文文献注重研究水蛭的有效成分水蛭素,包括分子结构、药理药效,以及治疗血栓、冠心病、肾病等疾病的作用机制等,同时关注不同种类水蛭活性成分的差异;英文文献除此之外还注重研究活体水蛭的临床外用。关键词突现提示,作用机制、水蛭素类似物的寻找和合成、配伍应用时的药效机制等不仅是目前的研究热点,也可能是未来的热点方向。结论水蛭成分分析、水蛭治疗心血管疾病、慢性肾脏病等疾病的药理作用机制研究始终是本领域研究热点;水蛭素类似物、配伍应用的药效机制、网络药理、分子对接等可能是水蛭的未来研究热点和趋势。
文摘As outdoor sports are loved by more and more people, the introduction of outdoor sports to the EE. teaching practice plays a positive role in enriching the contents of student sports activities. In this paper, the meanings and characteristics of outdoor sports are introduced, the necessities for the outdoor RE. teaching practice are analyzed, and finally several suggestions on the construction of the outdoor teaching practice in physical education are proposed.
文摘Objective THANK, known as a member of TNF superfamily, is a potent costimulator of both B and T lymphocytes and can promote astrong immune response. To investigate its role in liver immunotherapy, the anti-tumor effects of the THANK-transduced hepatoma cellline SMMU-7721 in vitro and in vivo were studied.Methods THANK full-length cDNA was transfected into SMMU-7721 cell line. The transfectant with stable expression of THANK wasobtained by clone selection and THANK s effects on hepatoma cells were analyzed, further the tumorigenicity of THANK-transduced7721 cells was examined in nude mice.Results THANK's expression in 7721 cells inhibited the growth of hepatoma cells and induced a strong CTL response in vitro. The cellcycle analysis showed that THANK transfected 7721 cells were arrested in the S phase. The expression of THANK in SMMU-7721 cellline not only inhibited the tumorigenicity of 7721 cells, but also induced a systemic immune response against re-challenge of parental7721 tumors.Conclusion THANK transduction in SMMU-7721 cells can induce an effective immune response in nude mice and may be useful for theimmunotherapy of hepatomas.
基金supported in part by the National Key S&T Special Projects on Major Infectious Diseases (Grant No. 2008ZX10001-002, 2008ZX10001-012)the National Natural Science Foundation of China (No. 30700706)
文摘Neutralizing antibodies are considered to be an important protective parameter used in HIV-1 vaccine evaluation. However, the exact role that neutralizing antibodies plays in controlling the disease progression of HIV-1 infected peoples is still undetermined. In this paper, we compared the protective function of the neutralizing antibody response in the plasma from LTNP and TP against clade B and clade C pseudoviruses. No difference in the neutralizing activities between the plasma from LTNP and TP was found, which was consistent with the most recent reports. In addition, no correlations between the titer or breadth and CD4+ or viral load in HIV-1 infected individuals were found. The protective roles played by neutralizing antibodies in controlling disease progression of HIV-1 infected people need to be considered in a new viewpoint.
基金supported by the National Natural Science Foundation of China (Grant Nos. 41402041 & 41322015)the Fundamental Research Funds for the Central Universities of China
文摘The combination of magnetotelluric survey and laboratory measurements of electrical conductivity is a powerful approach for exploring the conditions of Earth's deep interior. Electrical conductivity of hydrous silicate melts and aqueous fluids is sensitive to composition, temperature, and pressure, making it useful for understanding partial melting and fluid activity at great depths. This study presents a review on the experimental studies of electrical conductivity of silicate melts and aqueous fluids, and introduces some important applications of experimental results. For silicate melts, electrical conductivity increases with increasing temperature but decreases with pressure. With a similar Na^+ concentration, along the calc-alkaline series electrical conductivity generally increases from basaltic to rhyolitic melt, accompanied by a decreasing activation enthalpy. Electrical conductivity of silicate melts is strongly enhanced with the incorporation of water due to promoted cation mobility. For aqueous fluids, research is focused on dilute electrolyte solutions. Electrical conductivity typically first increases and then decreases with increasing temperature, and increases with pressure before approaching a plateau value. The dissociation constant of electrolyte can be derived from conductivity data. To develop generally applicable quantitative models of electrical conductivity of melt/fluid addressing the dependences on temperature, pressure, and composition, it requires more electrical conductivity measurements of representative systems to be implemented in an extensive P-T range using up-to-date methods.
