OBJECTIVE: To evaluate the anti-infectious efficacy of essential oil extracted from Caoguo(Fructus Tsaoko).METHODS: Minimum inhibitory concentrations(MICs) against clinical isolates of three extracts andthe essential ...OBJECTIVE: To evaluate the anti-infectious efficacy of essential oil extracted from Caoguo(Fructus Tsaoko).METHODS: Minimum inhibitory concentrations(MICs) against clinical isolates of three extracts andthe essential oil from Caoguo(Fructus Tsaoko) were determined by the agar dilution method. The anti-infectious efficacy of the essential oil was evaluated using a mouse peritonitis model which was infected with Staphylococcus aureus or Escherichia coli. The chemical components of the essential oil were identified.RESULTS: The results showed that the essential oil exhibited strong antibacterial activity in vitro, with MICs ranging from 22.49 to 1438.91 μg/m L. The results of in vivo anti-infectious efficacy showed that the Caoguo(Fructus Tsaoko) essential oil can protect the mice from Staphylococcus aureus or Escherichia coli infection. The compositions of the essential oil and relative component percentages were examined. A total of 32 compounds, were identified. The major compounds of essential oil were 1,8-cineole(25.92%) and geraniol(13.69%).CONCLUSION: Our findings suggest that Caoguo(Fructus Tsaoko) essential oil has broad-spectrum antibacterial properties. It warrants further investigation as an antibacterial agent targeting some bacterium with multi-drug resistance.展开更多
基金Supported by the Fund of Department of Science and Technology of Sichuan Province(Molecular Mechanism of Caoguo Oil Reversing Multi-Drug Resistance of MRSA by Regulating Mec A Gene Expression Through Mecr1-Meci-Meca Resistance Pathway,No.2016JY0014Study on Pharmacodynamics and Mechanism of Caoguo in Treatment of Infectious Diseases,No.2016FZ0068)+3 种基金Sichuan Province Office of Education(Bacterial Infection and Laboratory Diagnosis,No.16TD0027Research on the Antibacteria Mechanism of Geraniol,No.15ZB0238)the Open-Study Funds of State Key Laboratory Breeding Base of Systematic Research,Development and Utilization of Chinese Medicine,Chengdu University of Traditional Chinese Medicine(the Activity and Mechanism of Caoguo Oil Reversing Multi-Drug Resistance of MRSA)the Scientific Research Fund of Chengdu Medical College(the Study on the Synergistic Effect and the Mechanism of Geraniol andβ-lactam Antibiotics against Methicillin-resistant Staphylococcus Aureus,No.CYZ15-02)
文摘OBJECTIVE: To evaluate the anti-infectious efficacy of essential oil extracted from Caoguo(Fructus Tsaoko).METHODS: Minimum inhibitory concentrations(MICs) against clinical isolates of three extracts andthe essential oil from Caoguo(Fructus Tsaoko) were determined by the agar dilution method. The anti-infectious efficacy of the essential oil was evaluated using a mouse peritonitis model which was infected with Staphylococcus aureus or Escherichia coli. The chemical components of the essential oil were identified.RESULTS: The results showed that the essential oil exhibited strong antibacterial activity in vitro, with MICs ranging from 22.49 to 1438.91 μg/m L. The results of in vivo anti-infectious efficacy showed that the Caoguo(Fructus Tsaoko) essential oil can protect the mice from Staphylococcus aureus or Escherichia coli infection. The compositions of the essential oil and relative component percentages were examined. A total of 32 compounds, were identified. The major compounds of essential oil were 1,8-cineole(25.92%) and geraniol(13.69%).CONCLUSION: Our findings suggest that Caoguo(Fructus Tsaoko) essential oil has broad-spectrum antibacterial properties. It warrants further investigation as an antibacterial agent targeting some bacterium with multi-drug resistance.
