目的研究常用有机溶剂对正常男性精子体外运动活力的影响。方法取正常男性精子,采用上游优化法处理,制备成精子悬液并分成4组,前3组分别加入二甲亚砜,使二甲亚砜终浓度分别为0.5%,1%和2%,pH值均为7.5,渗透压为290 mosm.(kg.H2O-1),D组...目的研究常用有机溶剂对正常男性精子体外运动活力的影响。方法取正常男性精子,采用上游优化法处理,制备成精子悬液并分成4组,前3组分别加入二甲亚砜,使二甲亚砜终浓度分别为0.5%,1%和2%,pH值均为7.5,渗透压为290 mosm.(kg.H2O-1),D组加入等量Ham’s F10培养液作对照。分别于孵育15,30,45和60 m in后采用CASA进行分析。参照上述方法,考察有机溶剂甘油、聚乙二醇、吐温80对精子体外运动活力的影响。甘油终浓度分别调至1%,5%和10%,聚乙二醇终浓度分别调至1%,5%和10%,吐温80终浓度分别调至1%,5%和10%。结果2%二甲亚砜作用于精子60 m in能导致精子全部死亡,10%甘油作用60 m in后对精子运动参数抑制率达73.8%,10%聚乙二醇400作用于人精子60 m in可致其全部死亡,10%吐温80作用60 m in后对精子运动参数抑制率达95.5%。加入不同浓度二甲亚砜、甘油、聚乙二醇400和吐温80的各组前向运动精子百分率、曲线运动速度、直线运动速度和平均路径速度均较空白对照组显著降低(均P<0.05)。结论高浓度有机溶剂体外可抑制正常男性精子存活率和运动活力。展开更多
We studied the locomotor behaviour of white-headed langurs Trachypithecus leucocephalus and Francois' langurs T.francoisi to test two hypotheses: (1) these monkeys have evolved locomotor ability to support their a...We studied the locomotor behaviour of white-headed langurs Trachypithecus leucocephalus and Francois' langurs T.francoisi to test two hypotheses: (1) these monkeys have evolved locomotor ability to support their activities on limestone hills, and (2) Francois' langurs have evolved more diverse locomotor skills than white-headed langurs. Data were collected from 1996 - 1998 and in 2005 in Fusui Nature Reserve, Guangxi, and showed that the two species had similar locomotor types, but Francois' langurs had more locomotor modes (26) than white-headed langurs (12). Quadrupedal walking and leaping were two major types, and white-headed langurs were more arboreal than Francois' langurs. We suggest that, while keeping their ancestral locomotor types, the two species have evolved new types allowing them to live on limestone cliffs. Compared to white-headed langurs, Francois' langurs have more diverse locomotor modes that probably have allowed them to live in more habitat types. As an evolutionary outcome, Francois' langurs have a larger distribution range .展开更多
Perfluorooctane sulfonate (PFOS) has emerged as one of the most concerning contaminants in recent years. This study aimed to investigate the acute toxicity effect of PFOS on sperm viability, kinematics and fertiliza...Perfluorooctane sulfonate (PFOS) has emerged as one of the most concerning contaminants in recent years. This study aimed to investigate the acute toxicity effect of PFOS on sperm viability, kinematics and fertilization success in zebrafish (Danio rerio). Sperm were activated in aqueous media containing a range of PFOS concentrations (0, 0.09, 0.9 and 9 mg/L). Viabilities and kinematics of the sperm exposed to different PFOS treatments were assessed via computer-assisted sperm analysis (CASA) at 20, 40, 60, and 80 s after activation. PFOS exposure decreased the percentage of motile sperm, the curvilinear velocity (VCL), and the mean angular displacement (MAD) of spermatozoa, but showed no influence on the straight- line velocity (VSL) or the angular path velocity (VAP). Furthermore, a significant decrease in fertilization success was observed in spermatozoa that were exposed to 0.9 mg/L PFOS or more. These findings indicate that PFOS pollution in natural aquatic environment may be a potential threaten to successful reproduction of fish.展开更多
The present study evaluated the ef fects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm mot...The present study evaluated the ef fects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm motility and the activities of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx), glutathione reductase(Gr), and lipid peroxidation(measured via malondialdehyde(MDA) content) were determined after the milt was stored at 4°C for 12 h, cryopreserved without cryoprotectant in 12% propylene glycol(PG), cryopreserved in 12% PG+0.1 mol/L trehalose, or cryopreserved in 12% PG spermatozoa but centrifuged to decant the supernatant prior to cryopreservation(only sperm cells were cryopreserved). After chilled storage or cryopreservation, the SOD, CAT and GPx activities were reduced in sperm cells and increased in seminal plasma in almost all treatments; sperm motility parameters were also decreased. However, the addition of trehalose into the cryoprotectant could signifi cantly improve the postthaw sperm quality as revealed by the sperm average path velocity. This improvement might be attributed to the function of trehalose in scavenging reactive oxygen species. Chilled storage and cryopreservation had signifi cant eff ects on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod.展开更多
According to JJF1059.2-2012 and CNAS-GL06, the measurement uncertainty of four indicators (semen volume, sperm motility and validity, progressive motility, abnormal sperm percentage) of boar fresh semen, as well as ...According to JJF1059.2-2012 and CNAS-GL06, the measurement uncertainty of four indicators (semen volume, sperm motility and validity, progressive motility, abnormal sperm percentage) of boar fresh semen, as well as its influencing factors, was analyzed. The results showed that the measurement uncertainty of semen volume and progressive motility caused by inspectors was low, and their measurement results reliability was high. There were small differences in measurement results of semen volume and progressive motility among inspectors. However, the measurement uncertainty of sperm motility and abnormal sperm percentage was relatively large, and great differences were found among different inspectors. Therefore, the training of inspectors' measuring sperm motility and abnormal sperm percentage should be strengthened, and the comparison of measurement results among inspectors should be performed in the future.展开更多
The coordinates of the marked points of joints and the changes of foot force in the human walk motion are obtained with systems of motion image acquisition and force measuring.The effects of kinematics and dynamics on...The coordinates of the marked points of joints and the changes of foot force in the human walk motion are obtained with systems of motion image acquisition and force measuring.The effects of kinematics and dynamics on the human lower limb in different loads(0,10,20,30 kg) and walking speeds(0.8,1.3,1.7 m/s) have been investigated using the human dynamics model,.The results were as follows.First,with the increasing load,the dorsiflexion angle of ankle joint increased during the procedure of toe-off,the range of motion of the knee joint decreased,the flexion angle of the hip joint increased,and oppositely,the extension angle of the hip joint decreased.Second,with the increasing speed,the range of motion of the ankle joint,the maximum flexion angle of the knee joint,the flexion angle,extension angle of the hip joint and the range of motion of hip joint increased.Finally,the torque and power of the ankle joint,the knee joint and the hip joint increased with the increasing speed and load.The results can provide an important basis for the mechanism design of lower limb power-assisted exoskeleton.展开更多
文摘目的研究常用有机溶剂对正常男性精子体外运动活力的影响。方法取正常男性精子,采用上游优化法处理,制备成精子悬液并分成4组,前3组分别加入二甲亚砜,使二甲亚砜终浓度分别为0.5%,1%和2%,pH值均为7.5,渗透压为290 mosm.(kg.H2O-1),D组加入等量Ham’s F10培养液作对照。分别于孵育15,30,45和60 m in后采用CASA进行分析。参照上述方法,考察有机溶剂甘油、聚乙二醇、吐温80对精子体外运动活力的影响。甘油终浓度分别调至1%,5%和10%,聚乙二醇终浓度分别调至1%,5%和10%,吐温80终浓度分别调至1%,5%和10%。结果2%二甲亚砜作用于精子60 m in能导致精子全部死亡,10%甘油作用60 m in后对精子运动参数抑制率达73.8%,10%聚乙二醇400作用于人精子60 m in可致其全部死亡,10%吐温80作用60 m in后对精子运动参数抑制率达95.5%。加入不同浓度二甲亚砜、甘油、聚乙二醇400和吐温80的各组前向运动精子百分率、曲线运动速度、直线运动速度和平均路径速度均较空白对照组显著降低(均P<0.05)。结论高浓度有机溶剂体外可抑制正常男性精子存活率和运动活力。
文摘We studied the locomotor behaviour of white-headed langurs Trachypithecus leucocephalus and Francois' langurs T.francoisi to test two hypotheses: (1) these monkeys have evolved locomotor ability to support their activities on limestone hills, and (2) Francois' langurs have evolved more diverse locomotor skills than white-headed langurs. Data were collected from 1996 - 1998 and in 2005 in Fusui Nature Reserve, Guangxi, and showed that the two species had similar locomotor types, but Francois' langurs had more locomotor modes (26) than white-headed langurs (12). Quadrupedal walking and leaping were two major types, and white-headed langurs were more arboreal than Francois' langurs. We suggest that, while keeping their ancestral locomotor types, the two species have evolved new types allowing them to live on limestone cliffs. Compared to white-headed langurs, Francois' langurs have more diverse locomotor modes that probably have allowed them to live in more habitat types. As an evolutionary outcome, Francois' langurs have a larger distribution range .
基金Supported by the National Natural Science Foundation of China(Nos.40632009,31300340)the Natural Science Foundation Project of Chongqing(No.cstc2015jcyjA20016)the Youth Top-notch Talent Cultivation Program of Chongqing Normal University(No.14CSBJ08)
文摘Perfluorooctane sulfonate (PFOS) has emerged as one of the most concerning contaminants in recent years. This study aimed to investigate the acute toxicity effect of PFOS on sperm viability, kinematics and fertilization success in zebrafish (Danio rerio). Sperm were activated in aqueous media containing a range of PFOS concentrations (0, 0.09, 0.9 and 9 mg/L). Viabilities and kinematics of the sperm exposed to different PFOS treatments were assessed via computer-assisted sperm analysis (CASA) at 20, 40, 60, and 80 s after activation. PFOS exposure decreased the percentage of motile sperm, the curvilinear velocity (VCL), and the mean angular displacement (MAD) of spermatozoa, but showed no influence on the straight- line velocity (VSL) or the angular path velocity (VAP). Furthermore, a significant decrease in fertilization success was observed in spermatozoa that were exposed to 0.9 mg/L PFOS or more. These findings indicate that PFOS pollution in natural aquatic environment may be a potential threaten to successful reproduction of fish.
基金Supported by the National Natural Science Foundation of China(Nos.31072212,41076100)the CAS Scientific and Technological Innovation Program for Cross and Cooperative Team,Marine Economy Innovative Development Project(No.12PYY001SF08)the National Key Basic Program of Science and Technology Platforms of Aquaculture Stock Resources,Shandong Technology Development Project(No.2013GHY11509)
文摘The present study evaluated the ef fects of chilled storage and cryopreservation on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod Gadus macrocephalus. Sperm motility and the activities of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GPx), glutathione reductase(Gr), and lipid peroxidation(measured via malondialdehyde(MDA) content) were determined after the milt was stored at 4°C for 12 h, cryopreserved without cryoprotectant in 12% propylene glycol(PG), cryopreserved in 12% PG+0.1 mol/L trehalose, or cryopreserved in 12% PG spermatozoa but centrifuged to decant the supernatant prior to cryopreservation(only sperm cells were cryopreserved). After chilled storage or cryopreservation, the SOD, CAT and GPx activities were reduced in sperm cells and increased in seminal plasma in almost all treatments; sperm motility parameters were also decreased. However, the addition of trehalose into the cryoprotectant could signifi cantly improve the postthaw sperm quality as revealed by the sperm average path velocity. This improvement might be attributed to the function of trehalose in scavenging reactive oxygen species. Chilled storage and cryopreservation had signifi cant eff ects on sperm motion characteristics, antioxidant enzyme activities, and lipid peroxidation in the Pacific cod.
文摘According to JJF1059.2-2012 and CNAS-GL06, the measurement uncertainty of four indicators (semen volume, sperm motility and validity, progressive motility, abnormal sperm percentage) of boar fresh semen, as well as its influencing factors, was analyzed. The results showed that the measurement uncertainty of semen volume and progressive motility caused by inspectors was low, and their measurement results reliability was high. There were small differences in measurement results of semen volume and progressive motility among inspectors. However, the measurement uncertainty of sperm motility and abnormal sperm percentage was relatively large, and great differences were found among different inspectors. Therefore, the training of inspectors' measuring sperm motility and abnormal sperm percentage should be strengthened, and the comparison of measurement results among inspectors should be performed in the future.
文摘The coordinates of the marked points of joints and the changes of foot force in the human walk motion are obtained with systems of motion image acquisition and force measuring.The effects of kinematics and dynamics on the human lower limb in different loads(0,10,20,30 kg) and walking speeds(0.8,1.3,1.7 m/s) have been investigated using the human dynamics model,.The results were as follows.First,with the increasing load,the dorsiflexion angle of ankle joint increased during the procedure of toe-off,the range of motion of the knee joint decreased,the flexion angle of the hip joint increased,and oppositely,the extension angle of the hip joint decreased.Second,with the increasing speed,the range of motion of the ankle joint,the maximum flexion angle of the knee joint,the flexion angle,extension angle of the hip joint and the range of motion of hip joint increased.Finally,the torque and power of the ankle joint,the knee joint and the hip joint increased with the increasing speed and load.The results can provide an important basis for the mechanism design of lower limb power-assisted exoskeleton.