目的观察四君子汤含药血清对胃癌MGC803细胞活化T细胞核因子5(Nuclear factor of the activated T cell 5,NFAT5)及凋亡因子表达的影响,进一步探讨四君子汤抗肿瘤的作用机制。方法SD大鼠随机分为4组,依次为空白对照组(生理盐水等体积剂...目的观察四君子汤含药血清对胃癌MGC803细胞活化T细胞核因子5(Nuclear factor of the activated T cell 5,NFAT5)及凋亡因子表达的影响,进一步探讨四君子汤抗肿瘤的作用机制。方法SD大鼠随机分为4组,依次为空白对照组(生理盐水等体积剂量灌胃),四君子汤低剂量组(0.246 g·kg^(-1))、中剂量组(0.492 g·kg^(-1))和高剂量组(0.984 g·kg^(-1)),各组连续灌胃10 d,末次给药1.5 h后,行腹主动脉取血,收集血清,过滤后制备高、中、低剂量四君子汤含药血清。采用细胞计数试剂盒(CCK-8)法检测各组MGC803细胞增殖水平,流式细胞术检测各组MGC803细胞凋亡率,Real-time PCR法检测各组MGC803细胞B淋巴细胞瘤-2(B-cell lymphoma-2,Bcl-2)、半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)及NFAT5 mRNA的表达。结果与空白对照组比较,四君子汤中、高剂量组含药血清在24、48、72 h均能降低细胞增殖能力(P<0.05),且48、72 h抑制效果更为显著。与空白对照组比较,四君子汤中、高剂量组含药血清细胞凋亡率升高(P<0.05)。与空白对照组比较,四君子汤低、中、高剂量组含药血清的Bcl-2 mRNA表达降低,四君子汤低、中、高剂量组含药血清的Caspase-3 mRNA表达升高,四君子汤中、高剂量组含药血清的NFAT5 mRNA表达降低,差异均有统计学意义(P<0.05)。结论中、高剂量四君子汤含药血清能够促进Caspase-3表达,抑制NFAT5 mRNA、Bcl-2 mRNA的表达,抑制胃癌MGC803细胞增殖并促进其凋亡发生,发挥抗肿瘤作用。展开更多
Background: Immunoglobulins have immune-modulating capacities and are used for the treatment of different dermatological diseases. They have also been reported for the treatment of severe atopic dermatitis (AD). Objec...Background: Immunoglobulins have immune-modulating capacities and are used for the treatment of different dermatological diseases. They have also been reported for the treatment of severe atopic dermatitis (AD). Objectives: To determine the effects of immunoglobulins on the phenotype and function of peripheral T and B lymphocytes from patients with AD in comparison with healthy donors (HD) as controls. Methods: We studied lymphocyte activation and T-cell cytokine production from 12 patients with AD and 10 HD by multicolour flow cytometric analysis in the presence of immunoglobulins. Results: Immunoglobulins significantly inhibited T-cell activation (CD69), by 71%(AD) and by 62%(HD). Production of interferon-γand interleukin-4 was also significantly inhibited, by 44%/24%(AD) and 38%/10%(HD), respectively. In addition, CD86 expression on B lymphocytes was downregulated by 30%in AD and by 29%in HD, whereas CD23 expression was decreased without reaching statistical significance. Conclusions: Our data demonstrate that, in vitro, immunoglobulins modulate the activation and cytokine production of peripheral blood lymphocytes from both HD and patients with AD.展开更多
文摘Background: Immunoglobulins have immune-modulating capacities and are used for the treatment of different dermatological diseases. They have also been reported for the treatment of severe atopic dermatitis (AD). Objectives: To determine the effects of immunoglobulins on the phenotype and function of peripheral T and B lymphocytes from patients with AD in comparison with healthy donors (HD) as controls. Methods: We studied lymphocyte activation and T-cell cytokine production from 12 patients with AD and 10 HD by multicolour flow cytometric analysis in the presence of immunoglobulins. Results: Immunoglobulins significantly inhibited T-cell activation (CD69), by 71%(AD) and by 62%(HD). Production of interferon-γand interleukin-4 was also significantly inhibited, by 44%/24%(AD) and 38%/10%(HD), respectively. In addition, CD86 expression on B lymphocytes was downregulated by 30%in AD and by 29%in HD, whereas CD23 expression was decreased without reaching statistical significance. Conclusions: Our data demonstrate that, in vitro, immunoglobulins modulate the activation and cytokine production of peripheral blood lymphocytes from both HD and patients with AD.
